排序方式： 共有11条查询结果，搜索用时 15 毫秒
1.
Jawahar Lal Katara Ram Lakhan Verma Debkanta Nayak Umakanta Ngangkham Soham Ray Hatanath Subudhi Lambodar Behera Sanghamitra Samantaray Ravi Nageswara Rao Onkar Nath Singh Trilochan Mohapatra 《Plant Breeding》2017,136(1):74-82
Identification of new parental lines is crucial for developing ecology‐specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology‐specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3‐10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga‐based CMS lines. All the 80 F1s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding. 相似文献
2.
Nickel (0.13, 0.25, 0.5, 1.0 and 1.5 mg/l) increased the efficiency of somatic embryogenesis in leaf base and mesocotyl derived
calli of Setaria italica. A lower concentration of nickel in the culture media promoted long-term maintenance of embryogenic
calli that regenerated into plantlets. The plants obtained from embryogenic calli grown on Ni-containing medium showed tolerance
to nickel. The growth of embryogenic callus was the maximum at 1.5 mg/l as compared to a lower or a higher concentration of
Ni. Use of nickel may help in the induction of high frequency somatic embryogenesis in Setaria italica.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Summary Theileria infection of Hyalomma ticks collected from three districts of Haryana was assessed in whole salivary glands by the
methyl-green pyronin staining method. Of 1,662 ticks screened, 546 (32·8%) were found Theileria positive. Infection rate in
935 female ticks (36·9%) was more than that of 727 male ticks (27·6%). Density of Theileria infection (number of infected
acini per infected tick) was also higher in female ticks.Theileria infection rate of ticks varied greatly in the three districts viz. only 12·0% in Rohtak, 25·8% in Hisar and 48·3% in Karnal.
Per cent infection rate was high (63·7%) in ticks from indigenous cattle and low (18·6%) in those collected from buffaloes.
However, the intensity of infection in infected ticks from cross-bred cattle was comparatively much higher. Frequency distribution
ofTheileria positive acini in infected ticks revealed a low density of infection per infected tick. This points to the largely stable
endemic situation prevailing in Haryana. Only a single salivary acinus was found infected in 16·6% of the infected ticks,
about 70% had up to 10 infected acini while only about 10% had over 25 infected acini per tick. The masses in acini presumed
to beTheileria were confirmed by demonstrating parasitic masses on staining one of a pair of salivary glands and inoculating the suspension
of the other half of the gland into two cross-bred cow calves which developed clinical signs and lesions typical of theileriosis.
Resumen Se determinó la infectividad theilérica de garraptasHyaloma colectadas en tres distritos de Haryana, examinando las glándulas salivares mediante el método de coloración de la pironina verde-metilo. De 1662 ácaros examinados, 546 (32·8%) se encontraron positivas a theileria. La frecuencia de infección en 935 hembras (36·9%) fue más alta que la encontrada en 727 machos (27·6%). El número de acinos infectados por garrapata infectada fue tambien superior en garrapatas hembras. La fracuencia de la infección theilérica vario en tres distritos. Se encontro el 12·0% en Rohtak, 25·8% en Hisar y 48·3% en Karnal. La frequencia de infección por ciento fue alta (63·7%) en garrapatas de ganado criollo y bajo (18·6%) en aquellas colectadas de búfalos. Sin embargo, la frecuencia de infección en garrapatas de ganado cruzado, fue comparativamente más alta. La frecuencia de distribución de acinos positivos a theileria en garrapatas infectadas, reveló una baja densidad de infección por garrapata infectada. Esto se?ala una situación endémica estable prevalente en Haryana. Solamente un acino salivar se encontro infectado en el 16·6% de gappapatas infectadas, cerca del 70·0% tuvo arriba de 10 acinos infectados, mientras que solamente et 10·0% tuvo sobre los 25 acinos infectados, por garrapata. Las masas de acinos presumiblemente de theileria, se confirmaron mediante la tinción de la glándula salivar e inoculando parte de ella en terneros cruzados, los cuales desarrollaron theileriosis.
Résumé L’infection theilerienne desHyalomma récoltées dans 3 districts de l’Haryana a été examinée sur les glandes salivaires totales par la méthode de coloration au vert de méthyle-pyronine. Sur 1662 tiques, 546 (soit 32,8 p. 100) ont été trouvées positives pour lesTheileria. Le taux d’infection (36,9 p. 100) de 935 tiques femelles est supérieur à celui de 727 males (27,6 p. 100). La densité de l’infection parTheileria (nombre d’acini infectés par tique infectée) est plus grande chez les tiques femelles. Le taux d’infection des tiques varie considérablement selon le district: 12,0 p. 100 dans le Rohtak, 25,8 p. 100 dans l’Hisar et 48,3 p. 100 dans le Karnal. Il est élevé (63,7 p. 100) chez les tiques collectées sur du bétail indigène et bas sur celles prélevées sur des buffles (18,6 p. 100). Cependant, l’intensité de l’infection des tiques prélevées sur du bétail métis est comparativement plus grand. La fréquence de distribution des acini positifs pour lesTheileria chez les tiques infectées révèle une faible intensité d’infection par tique. Ceci montre la situation endémique stable existant dans l’Haryana. On n’a trouvé qu’un seul acinus infecté chez 16,6 p. 100 des tiques infectées; environ 70 p. 100 ont jusqu’à 10 acini infectés tandis que 10 p. 100 ont plus de 25 acini infectés. Les masses présentes dans les acini et présumées être desTheileria ont été confirmées être ces agents par coloration de l’une des paires de glandes salivaires et inoculation de l’autre à deux veaux métis qui ont developpé des signes cliniques et des lésions typiques de theileriose.相似文献
4.
Sanghamitra Samantaray Arunkumar Phurailatpam Ashok Kumar Bishoyi K. A. Geetha Satyabrata Maiti 《Genetic Resources and Crop Evolution》2012,59(5):645-653
The Random Amplified Polymorphic DNA (RAPD) technique was used to amplify DNA segments, with the objective of finding markers linked to sex determination in male and female plants of Piper betle L. Two bulks of DNA were made drawing one each from male and female, by pooling an equal volume of DNA samples from each group of individual contributing to the bulk analysis. Fifty different random decamer primers were screened with the two bulks to identify markers associated with sex expression of which only four primers were found to be associated with sex expression. These four primers were then tested with individual plant DNA samples where sex-associated RAPD markers were identified. A ~1,400 and ~850?bp fragment from the primer OPA04 and OPN 02 respectively was found to be present in all the male individuals and absent in all the female plants. In another primer, a ~980?bp amplification product from the primer OPC 06 was present only in the female individuals. A common primer OPA 08 showed both male and female specific markers of 650 and 1,200?bp respectively. Thus, the three male- specific RAPD markers OPA041400, OPA08650 and OPN02850 and two female-specific markers OPA081200 and OPC06980 can reliably differentiate the male and female plants of P. betle L. Ploidy comparison also showed the differences in male and female plants. 相似文献
5.
Chinmayee Priyadarshini Khuntia Basant Kumar Das Biswa Ranjan Samantaray Surya Kanta Samal & Bibhutendu Kumar Mishra 《Aquaculture Research》2008,39(3):301-310
Vibrio parahaemolyticus, an important pathogen of freshwater prawn, Macrobrachium rosenbergii causes high mortality. Four strains of V. parahaemolyticus were isolated from the gill, haepatopancreas, hemolymph and carapace of diseased prawn. The species were characterized based on biochemical and serological tests. On performing an antibiotic assay, these strains were found to be highly susceptible to cephalaxin, tetracycline and erythomycin. During the in vitro pathogenicity test, all the strains were found to be positive to the Congo red binding assay and to be haemolytic in nature, whereas an in vivo pathogenicity test revealed that 2 × 109 CFUs mL?1of bacteria induced disease symptoms such as black colouration on the carapace, red discolouration of the exoskeleton and loss of appendages within 6 days and 80% mortality. In histopathological studies, a prominent necrosis was seen in gill lamellae, and branchial arches were thickened at places due to hyperplasia and haemocytic infiltration. Hepatopancreatic tissue showed dilation of tubules, vacuolation of hepatocytes and marked necrosis in acinar cells. Development of an immunological technique for the detection and screening of V. parahaemolyticus infection and its treatment is highly important in freshwater prawn aquaculture. 相似文献
6.
7.
Summary Crossbred calves (Bos taurus × Bos indicus) were repeatedly infested with the ixodid tick,Hyalomma anatolicum anatolicum. The parameters of engorgement, pre-oviposition period, oviposition period, engorged weight, egg batch weight, and per cent
hatch of eggs all showed statistically significant differences between first to tenth infestation. Only the time to engorgement
did not change. It is concluded that repeated infestations of this tick stimulate acquired resistance.
Resumen Terrneros cruzadosBos taurus × Bos indicus se infestaron de manera natural. Con la grrapata ixodes,Hyalomma anatolicum anatolicum. Los parámetros medidos de ingurgitación, período de pre-oviposición, período de oviposición, peso de ingurgitación, peso del bache de huevos puestos y el porcentaje de eclosión de los mismos, presentaron diferencias estadísticamente significativas entre la primera y décima infestación. Lo único que no cambió fue el tiempo de ingurgitación. Se sugiere que la infestación repetida de esta garrapata, estimula la resistencia adquirida.
Résumé Des veaux issue de croisementsBos taurus × Bos indicus ont été soumis à une infestation répétée parHyalomma anatolicum anatolicum. Les paramètres suivants: gorgement, périodes de préet d'oviposition, poids en fin de gorgement, poids du lot de ponte et pourcentage d'oeufs éclos, ont tous montré des différences significatives entre la première et la dixième infestation; seule la durée de gorgement n'a pas varié. Les auteurs concluement que les infestations répétées par cette tique stimulent la résistance acquise des veaux.相似文献
8.
Eight mungbean (Vigna radiata L.) cultivars were evaluated for their tolerance to different levels of chromium (Cr+6) (0, 24, 48, 96, 192, and 384 μM) in nutrient solutions at pH 6.8. Seeds were germinated and grown in the presence of chromium under controlled environmental conditions. Standard growth parameters such as root length, shoot length, root/shoot dry matter production, and root/shoot tolerance index were used as indicators of chromium toxicity. Measurements as early as 24 h after the beginning of the treatments did not yield consistent results. However, root measurements at 48, 72, and 96 h after the beginning of treatments yielded significant differences among the cultivars which were similar to field performance observations made for mungbean growing in chromium‐rich soils. The cultivars, TARM‐22 and K‐851 had enhanced root growth while PDM‐54, Sujata, TARM‐21, LGG‐407, and PDM‐116 showed a reverse trend in root growth in the presence of chromium. The root tolerance index (RTI) and the shoot tolerance index (STI) with respect to the cultivars TARM‐22 and K‐851 were high indicating their tolerance to chromium, while the cultivars PDM‐54 and Sujata showed a low RTI and STL Based on the growth parameters of the eight mungbean cultivars, they were ranked with respect of their tolerance to chromium as follows: TARM‐22>K‐851>Dhauli>PDM‐116>LGG‐407>TARM‐21> Sujata>PDM‐54. Therefore, the nutrient culture method was able to quickly screen the mungbean cultivars for their tolerance to chromium. 相似文献
9.
Interacting effects of dietary lipid level and temperature on growth, body composition and fatty acid profile of rohu, Labeo rohita (Hamilton) 总被引：1，自引：0，他引：1
Three isonitrogenous (320 g kg?1 crude protein, casein and gelatine) semi‐purified diets with 80 (L8), 130 (L13) and 180 (L18) g kg?1 lipid (sunflower oil at increasing levels and cod liver oil fixed at 50 g kg?1) at three digestible energy levels (12 096, 13 986 and 15 876 kJ kg?1 dry weight) and were tested, in triplicate, on rohu fingerlings (3.2 ± 0.08 g) at two different temperatures (21 and 32 °C). Fish were fed to apparent satiation, twice daily, at 09.00 and 15.00 h, 7 days a week for 56 days. Maximum growth was obtained at a lipid level of 80 g kg?1 (L8) at 21 °C (439.37%) and 130 g kg?1 (L13) at 32 °C (481.8%). In general growth rate was higher at 32 °C than at 21 °C at all lipid levels. Tissue monounsaturated fatty acid (MUFA) contents decreased with increasing lipid level at 32 °C, but the reverse occurred at 21 °C. At 21 °C, Polyunsaturated fatty acid (PUFA) level increased significantly (P > 0.05) over initial values, but was affected insignificantly by dietary lipid level. At 32 °C, fish fed diet L13 had more n‐3 fatty acid (FA) in liver and muscle than the other two dietary groups while at 21 °C, both liver and muscle FA profiles exhibited significant change (P > 0.05) in n‐3 and n‐6 FA content which corresponded to variation in percent addition of dietary lipid. However, n‐3/n‐6 ratio was higher for fish fed diet L13 at 32 °C and diet L8 at 21 °C and may be correlated with fish growth. 相似文献
10.
The relative role of male and female Hyalomma anatolicum anatolicum ticks in the epidemiology of Theileria transmission was studied by detecting Theileria sporozoites in the dissected salivary glands of 568 ticks by the methyl green pyronin staining method. Detailed frequency distribution of Theileria-positive acini in the salivary glands of the 264 (46.48%) positive ticks from a field collection in Haryana indicated that the number of infected salivary acini per positive tick was greater in females than in males. This suggests that female ticks have a more important role in Theileria transmission than male ticks. This finding assumes greater significance in the light of the observation that the natural male:female ratio is also in favour of female ticks. 相似文献