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Jawahar Lal Katara Ram Lakhan Verma Debkanta Nayak Umakanta Ngangkham Soham Ray Hatanath Subudhi Lambodar Behera Sanghamitra Samantaray Ravi Nageswara Rao Onkar Nath Singh Trilochan Mohapatra 《Plant Breeding》2017,136(1):74-82
Identification of new parental lines is crucial for developing ecology‐specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology‐specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3‐10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga‐based CMS lines. All the 80 F1s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding. 相似文献
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Drought is a major abiotic constraint for rice production worldwide. The quantitative trait loci (QTLs) for drought tolerance traits identified in earlier studies have large confidence intervals due to low density linkage maps. Further, these studies largely focused on the above ground traits. Therefore, this study aims to identify QTLs for root and shoot traits at the vegetative growth stage using a genotyping by sequencing (GBS) based saturated SNP linkage map. A recombinant inbred line (RIL) population from a cross between Cocodrie and N-22 was evaluated for eight morphological traits under drought stress. Drought was imposed to plants grown in 75 cm long plastic pots at the vegetative growth stage. Using a saturated SNP linkage map, 14 additive QTLs were identified for root length, shoot length, fresh root mass, fresh shoot mass, number of tillers, dry root mass, dry shoot mass, and root-shoot ratio. Majority of the drought responsive QTLs were located on chromosome 1. The expression of QTLs varied under stress and irrigated condition. Shoot length QTLs qSL1.38 and qSL1.11 were congruent to dry shoot mass QTL qDSM1.38 and dry root mass QTL qDRM1.11, respectively. Analysis of genes present within QTL confidence intervals revealed many potential candidate genes such as laccase, Calvin cycle protein, serine threonine protein kinase, heat shock protein, and WRKY protein. Another important gene, Brevis radix, present in the root length QTL region, was known to modulate root growth through cell proliferation and elongation. The candidate genes and the QTL information will be helpful for marker-assisted pyramiding to improve drought tolerance in rice. 相似文献
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Dynamics of cytokine gene expression in peripheral blood mononuclear cells of indigenous and exotic breeds of pigs in India 
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Sarah E. Bertrand-Garcia Carrie A. Knott Niranjan Baisakh Prasanta K. Subudhi Stephen A. Harrison Michael D. Materne Herry Utomo 《Euphytica》2012,185(1):103-117
Numerous sea oats (Uniola paniculata L.) plants are transplanted to Northern Gulf of Mexico beaches each year to reduce coastal erosion. To adapt to environmental
changes and effectively reduce coastal erosion, genetically diverse sea oats plants with demonstrated plant performance must
be used. The objectives of this study were to: (i) identify improved sea oats lines; and (ii) determine the genetic diversity
of improved sea oats lines. From 2003 to 2005, 2,000 sea oats lines were evaluated in unreplicated field trials at natural
beach sites. In 2005, 75 sea oats lines were selected based upon phenotypic performance. The 75 selected lines and 3 plants
of ‘Caminada’, the only commercially available sea oats line, were evaluated in replicated field trials in 2008 and 2010.
In 2008, UP01LA-15 K-HB-3092, UP01LA-16S-GP-3138, UP01LA-31-GP-3103, UP01LA-33-GP-1303, and UP01NC-04-HB-3374 had higher (p < 0.05) stem densities than Caminada. In 2010, variation was not detected for any trait measured; lack of significant differences
was most likely due to plant stress caused by storm surge one week after transplant. Amplified Fragment Length Polymorphism
detected 534 loci for the 75 selected lines and 3 Caminada plants. One hundred eighty one loci were polymorphic; the average
polymorphism rate was 34% (range = 25–43%). Polymorphic information content values ranged from 0.15 to 0.41 and averaged 0.29
while Jaccard similarity coefficients ranged from 0.8243 to 0.9794. These findings demonstrate the application of plant breeding
techniques to develop genetically different sea oats lines with improved performance for coastal restoration projects in the
northern Gulf of Mexico. 相似文献
5.
Prasanta K. Subudhi Gerard B. Magpantay Ratna Karan 《Genetic Resources and Crop Evolution》2013,60(4):1263-1273
A genomic DNA fragment (pAA7-2) amplified by a random amplified polymorphic DNA primer from the rice cultivar IR68 was used to assess the genetic variation and genetic relationships among the species of genus Oryza. A Southern hybridization experiment of diverse upland rice accessions using a single restriction enzyme HindIII generated unique DNA fingerprint for each accession. The differential hybridization pattern reflecting the copy number variation of pAA7-2 in a collection of wild species and cultivated species of rice provided insight about the genetic relationships among them. All AA genome species exhibited clear banding pattern suggesting presence of fewer copies of this sequence. Strongest hybridization signal was obtained in species belonging to BB, CC, GG, BBCC, CCDD genomes, whereas weakest hybridization signal was visible in EE, FF, and HHJJ genome species. Oryza brachyantha was the most divergent species. Clear difference in banding pattern was evident between Oryza schlechteri and Oryza coarctata belonging to HHKK genome. Although pAA7-2 had no repetitive sequences often associated with hypervariable loci, homology to a putative unclassified expressed retrotransposon distributed over several rice chromosomes was responsible for the complex banding patterns. There were more sites homologous to pAA7-2 sequence in corn and sorghum genome compared with the rice genome. The study demonstrates the potential of pAA7-2 as a powerful molecular tool for DNA fingerprinting, genetic diversity, phylogenetic, and evolutionary studies in Oryza sativa and its wild relatives and other grasses. 相似文献
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Kylla H. Dutta T. K. Roychoudhury P. Malik Y. S. Mandakini R. Subudhi P. K. 《Tropical animal health and production》2017,49(2):417-422
Tropical Animal Health and Production - Picobirnaviruses (PBVs) have been recognized as one of the important causal viral agents of gastroenteritis in several animal species especially in young... 相似文献
9.
Arpita Moharana Aradhana Das Enketeswara Subudhi Soumendra K. Naik Durga P. Barik 《Journal of Crop Science and Biotechnology》2017,20(5):405-416
An efficient and reproducible protocol for in vitro plant regeneration was developed for Lawsonia inermis L. using cotyledonary node explant derived from axenic seedlings. Highest shoot proliferation frequency (ca 96.6%) was achieved on Murashige and Skoog’s, 1962 (MS) basal medium supplemented with 8.88 μM 6-Benzyladenine (BA) + 2.68 μM Napthalene acetic acid (NAA). Up-scaling of shoots was carried out using in vitro nodes on MS medium supplemented with 4.44 μM BA. So overall, an average of 238 shoots was produced at 75 days. Of the four different forms of cotyledonary node explants evaluated, highest shoot multiplication was observed in cotyledonary node explant with two whole cotyledons. In vitro regenerated shoots were best rooted (ca 34.3 roots / shoot) on ½ MS medium devoid of any growth regulator. The plantlets were successfully acclimated in sand:soil:: 1:1and established in the garden soil. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis revealed a homogeneous amplification profile for all micropropagated plants validating the genetic fidelity of the in vitro-regenerated plants and supporting the regeneration protocol for economic commercial exploitation. 相似文献
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Summary Three interspecific crosses made between Brassica juncea and Brassica napus revealed digenic control with epistatic interaction for white rust resistance trait. The investigation also indicated a close association of parental species type and different grades of leaf waxiness with white rust resistance. It is possible to recover waxy or medium waxy juncea types with white rust resistance, though in low frequency. Treatment of hybrid seeds with EDTA and low doses of gamma-rays seem to have little effect on shuffeling of genomes and genes.Abbreviations WR
White rust
- J
Juncea
- N
Napus
- I
Intermediate
- W
Waxy
- MW
Medium waxy
- G
Glossy 相似文献