Conductive 3D structure nanofibrous scaffolds for spinal cord regeneration     

Fatemeh Zamani  Mohammad Amani-Tehran  Arash Zaminy  Mohammad-Ali Shokrgozar 《Fibers and Polymers》2017,18(10):1874-1881

The complex nature of spinal cord injuries has provided much inspiration for the design of novel biomaterials and scaffolds which are capable of stimulating neural tissue repair strategies. Recently, conductive polymers have gained much attention for improving the nerve regeneration. In our previous study, a three-dimensional (3D) structure with reliable performance was achieved for electrospun scaffolds. The main purpose in the current study is formation of electrical excitable 3D scaffolds by appending polyaniline (PANI) to biocompatible polymers. In this paper, an attempt was made to develop conductive nanofibrous scaffolds, which can simultaneously present both electrical and topographical cues to cells. By using a proper 3D structure, two kinds of conductive scaffolds are compared with a non-conductive scaffold. The 3D nanofibrous core-sheath scaffolds, which are conductive, were prepared with nanorough sheath and aligned core. Two different sheath polymers, including poly(lactic-co-glycolic acid) PLGA and PLGA/PANI, with identical PCL/PANI cores were fabricated. Nanofibers of PCL and PLGA blends with PANI have fiber diameters of 234±60.8 nm and 770±166.6 nm, and conductivity of 3.17×10^-5 S/cm and 4.29×10^-5 S/cm, respectively. The cell proliferation evaluation of nerve cells on these two conductive scaffolds and previous non-conductive scaffolds (PLGA) indicate that the first conductive scaffold (PCL/ PANI-PLGA) could be more effective for nerve tissue regeneration. Locomotor scores of grafted animals by developed scaffolds showed significant performance of non-conductive 3D scaffolds. Moreover, the animal studies indicated the ability of two new types of conductive scaffolds as spinal cord regeneration candidates.  相似文献   

The effects of transportation stress on Japanese quail (Coturnix Coturnix japonica) fed corn‐based diet in comparison with wheat‐based diet supplemented with xylanase and phytase          下载免费PDF全文

M. H. Mehraei Hamzekolaei  A. K. Zamani Moghaddam  S. S. Tohidifar  A. Dehghani Samani  A. Heydari 《Journal of animal physiology and animal nutrition》2016,100(4):618-622

Harvesting, handling and transporting quails to the slaughterhouses, other farms and laboratories might covertly reduce their welfare. The aim of this study was to evaluate the effects of two major sources of energy in poultry nutrition on reducing transportation stress in Japanese quail (Coturnix Coturnix japonica). Male quails (n = 60) were divided into two groups. The first group was fed corn‐based diet, and the second was fed wheat‐based diet supplemented with xylanase and phytase. At the end of the experiment (day 35), quails were subjected to 80 km of transportation. Immediately on arrival and after 24 h, heterophil counts, lymphocyte counts and H:L ratios were measured. On arrival, H counts were lower, L counts were higher, and H:L ratios were lower for corn‐fed group. After 24 h, wheat‐fed group showed lower increment of H counts, greater increment of L counts and also decrement of H:L ratios rather than corn‐fed group which showed increment of H:L ratios. However, these ratios were still lower in corn‐fed group. Results indicate that corn‐based diets can help Japanese quail to better resist transportation stress, although it seems that feeding wheat‐based diets supplemented with xylanase and phytase could have positive effects for coping better with stress after journeys.  相似文献   

Field evaluation of glutathione and glutamic acid as host marking pheromones for control of Tephritid fruit flies in a mango orchard in Kenya     

Xavier Cheseto  Hillary Kirwa  Samira Abuelgasim Mohamed  Sunday Ekesi  John J Beck  Baldwyn Torto 《Pest management science》2023,79(4):1585-1592

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Farmer participation in irrigation management: The case of Doroodzan Dam Irrigation Network, Iran   总被引：1，自引：0，他引：1  

Taher Azizi Khalkheili  Gholam Hosein Zamani 《Agricultural Water Management》2009,96(5):859-865

The importance of farmer participation in system design and management has been emphasized in previous studies. The purpose of this study was to identify the factors affecting farmer participation in irrigation management using survey research. The study was conducted in Doroodzan Dam Irrigation Network in Fars province, Iran. Multistage stratified random sampling was used to collect data from 270 farmers as the research sample. Results reveal that farmers’ attitudes toward participation in irrigation management, attitudes toward personnel of the State Water Authority and the Agricultural Extension Service Centers (AESCs), family size, the problem perception, dependence on the dam for water, and educational background have influenced their participation in irrigation management. By contrast, contact with information sources, animal units, sociability, age and agricultural experience did not affect farmers’ participation. Moreover, based on farmers’ perspectives, unequal water distribution among farms, dissatisfaction with Water Authority operators and high water fees and charges were the main problems and obstacles toward farmer participation in irrigation management.  相似文献   

Dynamics of natural infection by Toxoplasma gondii in goat herds in the semiarid region of northeastern Brazil: transitional antibody observation     

Sousa  Larissa Nascimento  Batista  Samira Pereira  Silva  Samara Santos  da Silva  Rômulo Fylipe  Sarmento  Wlysse Ferreira  Mota  Rinaldo Aparecido  Brasil  Arthur Willian Lima  Amaku  Marcos  Feitosa  Thais Ferreira  Vilela  Vinícius Longo Ribeiro 《Veterinary research communications》2022,46(2):507-515

Veterinary Research Communications - This study aimed to describe the transmission of T. gondii in naturally infected goats in the semiarid region of northeastern Brazil, through evaluating the...  相似文献   

Designing E1 deleted adenoviral vector by homologous recombination   总被引：2，自引：0，他引：2  

Zamani A  Ghannad MS 《Iranian Biomedical Journal》2007,11(3):199-202

BACKGROUND: Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generation of large collection of clones. METHODS: In this project, to delete E1 gene in adenovirus, an adenoviral plasmid containing lateral sites of E1 region of adenovirus was made and recombination in the 293A cells between the homologous region of this linearized plasmid and the adenovirus genome resulted in the formation of the complete adenoviral recombinant. RESULTS: This recombination resulted in loss of E1 region and we constructed a recombinant adenovirus type 5 vector that E1 gene was deleted by homologous recombination. CONCLUSION: Homologous recombination is more easy and fast technique in the production of AdV.  相似文献   

Integrated pest management of Tuta absoluta: practical implementations across different world regions     

Desneux  Nicolas  Han  Peng  Mansour  Ramzi  Arn&#;  Judit  Br&#;vault  Thierry  Campos  Mateus R.  Chailleux  Anais  Guedes  Raul N. C.  Karimi  Javad  Konan  Kouassi Arthur J.  Lavoir  Anne-violette  Luna  Mar&#;a G.  Perez-Hedo  Meritxell  Urbaneja  Alberto  Verheggen  Fran&#;ois J.  Zappal&#;  Lucia  Abbes  Khaled  Ali  Abid  Bayram  Yunus  Cantor  Fernando  Cuthbertson  Andrew G. S.  De Vis  Raf  Erler  Fedai  Firake  Dnyaneshwar M.  Haddi  Khalid  Hajjar  M. Jamal  Ismoilov  Khasan  Jaworski  Coline C.  Kenis  Marc  Liu  Hao-tian  Madadi  Hossein  Martin  Thibaud  Mazih  Ahmed  J. Messelink  Gerben  Mohamed  Samira A.  Nofemela  Robert S.  Oke  Abiola  Ramos  C&#;sar  Ricupero  Michele  Roditakis  Emmanouil  Shashank  Pathour R.  Wan  Fang-Hao  Wang  Ming-hui  Wang  Su  Zhang  Yi-Bo  Biondi  Antonio 《Journal of pest science》2022,95(1):17-39

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato...  相似文献   

The parasitoid Dolichogenidea gelechiidivoris eavesdrops on semiochemicals from its host Tuta absoluta and tomato     

Ayelo  Pascal M.  Mohamed  Samira A.  Chailleux  Ana&#;s  Yusuf  Abdullahi A.  Pirk  Christian W. W.  Deletre  Emilie 《Journal of pest science》2022,95(2):633-652

Journal of Pest Science - Semiochemicals such as herbivore-induced plant volatiles (HIPVs) and host chemicals serve as communication signals for parasitoids searching for oviposition sites. The...  相似文献   

Effects of fishing practice changes on pelagic shark longline captures in the Gulf of Gabes,Tunisia     

Bechir Saidi  Sami Karaa  Samira Enajjar  Mohamed. N. Bradaï 《水产资源保护:海洋与淡水生态系统》2020,30(1):53-67

1. In order to increase their catches, longliners targeting sharks in the Gulf of Gabes, Tunisia, have increased the number of hooks per basket and have included new bait types instead of mackerel (Scomber scombrus). This paper reports the effects of these changes on catch composition, catch rates, size distribution, life‐stage captures, and mortality at haulback.
2. Data from 48 and 96 longline sets, carried out during the shark fishing seasons of 2007 and 2008, with two hooks per basket, and 2016 and 2017, with five hooks per basket, respectively, were examined to assess the effect of gear change. Moreover, the effects of bait types were inspected based on 33 fishing sets using whole mackerel, 19 using salema (Sarpa salpa) halves, and 27 using pieces of stingray (Dasyatis spp.), sampled during 2016 and 2017.
3. The species composition indicated that longliners expand the vertical distribution of their hooks to operate from the surface to the bottom. The catches with both longline designs were dominated by sandbar shark (Carcharhinus plumbeus). Despite gear changes, the catch rate of C. plumbeus decreased by 42.21%, suggesting population decline. The size composition indicated a shift towards larger specimens, mainly adult females, which could jeopardize the shark population. Moreover, the mortality of the main species at haulback seems to be affected by the design of the longline.
4. The bait used, mainly pieces of stingray, significantly increased the catch rate of sandbar shark, which suggests a greater attractiveness of the new bait; however, fish size and mortality rates at haulback were unchanged in response to bait variation.
5. The fishery operates in shark nursery grounds, which exposes these fish (principally C. plumbeus) to considerable exploitation pressure. The new fishing practices intensified the pressure and thus the risk of a rapid depletion of populations. To preserve the shark species in the area, the use of new gear and attractive bait should be banned.

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Are circle hooks effective management measures in the pelagic longline fishery for sharks in the Gulf of Gabès?     

Bechir Saidi  Khaled Echwikhi  Samira Enajjar  Sami Karaa  Imed Jribi  Mohamed N. Bradai 《水产资源保护:海洋与淡水生态系统》2020,30(6):1172-1181

1. This study evaluated the circle hook use as a tool for shark management in the pelagic longline fishery in the Gulf of Gabès.
2. The usual J‐hook No. 2 with 10° offset, which has been traditionally used by the fishery, was compared to the 18/0 non‐offset circle hook in an alternating fashion along the main line. In total, 22 experimental longline sets were deployed through the shark fishing seasons of 2016 and 2017 to examine the effects of hook types on the catch composition, the catch rates, the hooking location, and the status at haulback.
3. The catch composition differed significantly among hook types. Moreover, an overall increase in catch rates for the main species, the sandbar shark Carcharhinus plumbeus, and the shortfin mako shark Isurus oxyrinchus, was observed when using circle hooks.
4. There was no size‐selective effect of circle hooks for the common species. The circle hooks were not effective at reducing at‐haulback mortality of sandbar shark. Conversely, shortfin mako and smooth‐hound shark Mustelus mustelus showed significantly lower relative mortality at haulback with circle hooks than with J‐hooks. Furthermore, circle hooks were more frequently hooked externally than the J‐hooks for the three shark species.
5. Results demonstrated that the use of 18/0 non‐offset circle hooks in the pelagic shark longline fishery can reduce mortality at haulback for some species without any benefit for the dominant species, the sandbar shark.
6. Overall, it is difficult to promote the adoption of the use of circle hooks as a management measure in this specialized fishery. Management measures focusing on fishing effort controls, fishing closures in critical habitats, and size limits could have significant benefits for the conservation of shark species and may help to improve the sustainability of the shark fishery in the Gulf of Gabès.

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