姬松茸与双孢蘑菇不同菌株的RAPD扩增研究   总被引：6，自引：1，他引：6  

李三暑  林新坚  陈惠成  雷锦桂  林勇  江枝和 《食用菌学报》2002,9(3):1-4

以4个姬松茸菌株和2个双孢蘑菇菌株为材料，用20个随机引物对它们进行RAPD扩增，通过对供试菌株遗传相似系数的估算和系统聚类分析，构建姬松茸和双孢蘑菇遗传相关树状聚类图谱，结果表明，进行RAPD扩增的20个随机引物中，有12个能产生三种带型，当遗传相似系数升至0.8713时，这6个菌株被分为3类，第一类为菌株18和26，第二类为菌株13和12，第三类为菌株33和45。  相似文献   

水稻粒宽突变体gw87的基因定位及候选基因分析     

张翔宇  郭佳  王三  陈聪萍  孙昌辉  邓晓建  王平荣 《中国农业科学》2021,54(12):2487-2498

【目的】对水稻粒宽突变体gw87（grain width 87）进行表型鉴定、遗传分析、基因定位及候选基因分析,为探明该基因调控水稻籽粒大小的分子机制及应用潜力奠定基础。【方法】利用甲基磺酸乙酯诱变籼稻恢复系材料676R,获得一份籽粒宽度和千粒重显著增加的突变体gw87。对该突变体进行表型观察、农艺性状调查及外源油菜素内酯（BL）敏感性、叶绿素含量、光合参数测定,了解其表型特征及生理特性。调查gw87与676R杂交F₁的表型和F₂群体的分离情况,分析其遗传行为;选取该群体中的突变植株进行高通量测序,并利用gw87与粳稻品种日本晴杂交的F₂代作为定位群体,通过MutMap分析和分子标记定位,遴选候选基因并进行DNA和cDNA测序验证。利用qRT-PCR分析gw87和676R中BR合成途径基因OsDWARF4、D11和D2的表达差异。【结果】与野生型亲本676R相比,gw87突变体的株高降低,节间缩短,其中,倒一节间长度缩短最大,并呈现出扭曲的形态;叶片长度减少,宽度增加;单株有效穗数、主穗穗长和结实率显著降低,但籽粒宽度和千粒重显著增大。BL敏感性试验显示,gw87突变体幼苗对外源BL的敏感性降低。光合色素和光合参数测定表明,gw87光合色素含量增加,光合速率也有所增加。遗传分析表明gw87的突变性状是由1对隐性核基因控制。MutMap分析显示gw87突变基因位于第5染色体中部,在该染色体区域仅有1个碱基突变引起编码氨基酸变化;分子标记连锁分析表明该突变基因位于InDel标记X2和X3之间约101 kb的染色体区域;综合这两方面分析结果,最后遴选出gw87候选基因是编码一个含有AP2/EREBP DNA绑定结构域的转录因子基因LOC_Os05g32270。对该候选基因进行DNA和cDNA测序验证,发现gw87突变体中该基因DNA的第1 041位的碱基由G突变为A,导致与该位点相邻的76 bp内含子序列被剪接为外显子,引起阅读框移码,蛋白翻译提前终止。qRT-PCR分析显示gw87突变体中BR合成途径基因的表达量显著上调,表明gw87突变体中BR信号减弱。【结论】gw87是smos1、shb、rla1、ngr5的新等位突变体,但与这些突变体表型不同,gw87籽粒的宽度和千粒重显著增加,可能是LOC_Os05g32270的突变位点不同,导致其编码蛋白的功能活性不同所造成。  相似文献   

Comparative pharmacokinetics and pharmacokinetic/pharmacodynamic analysis by nonlinear mixed‐effects modeling of cefquinome in nonpregnant,pregnant, and lactating goats after intravenous and intramuscular administration     

Nicols Javier Litterio  Augusto Matías Lorenzutti  María del Pilar Zarazaga  Martín Alejandro Himelfarb  Manuel Ignacio San Andrs‐Larrea  Juan Manuel Serrano‐Rodríguez 《Journal of veterinary pharmacology and therapeutics》2021,44(1):68-78

Cefquinome is a fourth‐generation cephalosporin that is used empirically in goats. Different physiologic factors like pregnancy or lactation could determine the pharmacokinetic behavior of drugs in the organism. The objectives of this study are to (a) compare the pharmacokinetics of cefquinome after intravenous and intramuscular administration in adult nonpregnant (n = 6), pregnant (n = 6), and lactating goats (n = 6), at a dose of 2 mg/kg, with rich sampling by nonlinear mixed‐effects modeling, (b) conduct a pharmacokinetic/pharmacodynamic analysis to evaluate the efficacy of the recommended posology in goats with different physiological states, and (c) determine the optimal posology that achieve a PTA value ≥ 90%, taking into account a T > MIC ≥ 60% of a MIC value ≤ 0.25 µg/ml, in the different subpopulations of goats for both routes. Gestation significantly increased Ka and V1, while reduced F0, Cl, and Q. On the other hand, lactation significantly increased V1 and reduced Tk0. Cefquinome concentrations achieved in placental cotyledon, amniotic fluid, and fetal serum indicate a minimal penetration across the placental barrier. Moreover, milk penetration of cefquinome was minimal. The total body clearance of cefquinome for goats was 0.29 L kg^?1 hr^?1, that is apparently higher than the reported for cows (0.13 L kg^?1 hr^?1) and pigs (0.16 L kg^?1 hr^?1). So, the optimal dose regimen for cefquinome after intravenous and intramuscular administration required higher dose and frequency of administration compared with recommendations for cows or pigs. Therefore, 2 mg kg^?1 8 hr^?1 and 5 mg kg^?1 12 hr^?1 could be used for IV and IM routes, respectively, for the treatment of respiratory infections caused by P. multocida and M. haemolytica, but only 5 mg kg^?1 12 hr^?1 by both routes should be recommended for Escherichia coli infections.  相似文献   

Response of nutsedge (Cyperus esculentus L. var sativus Boeck.) tuber production to drip irrigation based on volumetric soil water content     

N. Pascual-Seva  A. San Bautista  S. López-Galarza  J. V. Maroto  B. Pascual 《Irrigation Science》2015,33(1):31-42

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Cloning and characterization of chicken growth hormone binding protein (cGHBP)     

Lau JS  Yip CW  Law KM  Leung FC 《Domestic animal endocrinology》2007,33(1):107-121

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用单克隆抗体结合酶联免疫吸附试验检测棉铃虫幼虫体内的核多角体病毒   总被引：1，自引：0，他引：1  

陆自强  胡广淦  陈丽芳  杨峰  杭三保  乐云仙  苏德明 《中国生物防治》1995,(1)

在常规ＥＬＩＳＡ间接法的基础上，应用单克隆抗体检测感染棉铃虫核型多角体病毒的棉铃虫幼虫体内病毒粒子。３龄幼虫饲喂表层涂有ＨａＮＰＶ人工饲料后９小时，即可在幼虫抽提液中检出病毒粒子抗原，而典型病虫显症需５～６天后才出现。因此本法是一种灵敏、快速、特异性强的检测昆虫杆状病毒的方法。利用单克隆抗体结合ＥＬＩＳＡ试验分别检测来自江苏和山东不同地区棉田自然死亡的棉铃虫幼虫，表明在江苏和山东不同棉区均可检测到ＨａＮＰＶ病毒粒子，但地区间棉铃虫核型多角体病毒检出频率有显著差异  相似文献   

作物根际和叶围中产几丁质酶微生物的分布及其抑制真菌作用     

陈三凤  李季伦 《中国生物防治》1994,(2)

从几十种作物的根际和叶围分离获得３５０株微生物，其中细菌和放线菌分别为２００株和１５０株。对所有这些分离物进行了抑制真菌试验和几丁质酶活性测定。在抑菌试验中，以棉花枯萎病菌、棉花黄萎病菌、黄瓜枯萎病菌，及西瓜枯萎病菌作为供试病原菌，有９６个分离物对其中的一种或一种以上的病原菌有抑制作用，其中１８个分离物对这４种病原菌都具有强烈的抑制作用。在几丁质酶活性方面，１０％的细菌和４０％的放线菌都能不同程度地产生几丁质酶。有些菌株几丁质酶活性强，抑菌作用也明显，有些菌株几丁质酶活性弱，抑菌作用弱或无作用，少数菌株几丁质酶活性强，但却无抑菌作用。用几丁质酶液处理病原菌孢子，其萌发率和芽管长度比对照显著降低  相似文献   

Large granular lymphocytic leukemia in a mixed breed dog          下载免费PDF全文

Lau KW  Kruth SA  Thorn CE  Vernau W  Moore P 《The Canadian veterinary journal. La revue veterinaire canadienne》1999,40(10):725-728

A mixed breed dog was diagnosed with large granular lymphocytic leukemia. Immunophenotypic analysis indicated the lymphocytes were CD3+, CD8+ T cells expressing the alpha beta T cell receptor and a leukointegrin, alpha d. Chemotherapy and splenectomy resulted in an initial reduction in the lymphocyte count.  相似文献   

Nutritional evaluation of sunflower seed and products derived from them. Effect of oil extraction     

San Juan LD  Villamide MJ 《British poultry science》2000,41(2):182-192

1. Apparent MEn and oil digestibility of hulled sunflower seed (SFS) and the products derived from the oil extraction process: press extracted SFS (PESFS), sunflower seed meal (SFSM), and press and solvent oils (PO and SO) were determined with 198 cockerels. Recombined products (mix of meal and oil) were also evaluated to study the effect of the 2 consecutive oil extraction steps. Each foodstuff was included in a basal diet, according to the proportions resulting from processing, at 100, 200, and 300 g SFS/kg. 2. Dietary energy value and digestible fat content were linearly related to rate of inclusion of test ingredients. Extrapolation values for AMEn (MJ/kg DM) were: SFS, 16.20; PESFS, 9.46; SFSM, 7.62. A decreasing quadratic trend was also found in the AMEn of PESFS, with interpolation values ranging from 11.77 to 9.33 MJ/kg DM between 70 and 210 g/kg DM of inclusion. No differences were observed between PO and SO. The AMEn of of sunflower oil, calculated from its digestibility, was 33.70 MJ/kg DM. 3. The oil extraction process affected the nutritional value of sunflower products. Recombined materials showed greater values than original foodstuffs: R-SFS, 17.47; R-PESFS, 11.49 MJ/kg DM. The increase in oil digestibility (from 0.814 to 0.862 g/kg DM in SFS; from 0.778 to 0.892 in PESFS) accounted for most of the increase observed in AMEn values. 4. As the form in which oil is incorporated in diets (released or within SFS or PESFS) affects the utilisation of sunflower products, their nutritional value is less than maximal and should not be calculated from their ingredients. Solvent oil seems to be the less available fraction of sunflower oil within SFS as the effect of the 2nd extraction proved to be greater than that of the previous press extraction.  相似文献   

试论邓小平法治观的时代精神     

邓三好 《湖南农机》2007,(5):111-112

邓小平法治观具有鲜明的时代精神和个性特色。即在强调法制中彰显法治的理念;在环境营造中重视人的素质提高;在法治实践中务求实效;在法治思想中透射世界眼光。这四个方面,正是邓小平法治思想的精髓所在。  相似文献