Serum 5'nucleotidase activity in rats: a method for automated analysis and criteria for interpretation     

Carakostas MC  Power RJ  Banerjee AK 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(4):109-113

A manual kit for determining serum 5'nucleotidase (5'NT, EC 3.1.3.5) activity was adapted for use with rat samples on a large discrete clinical chemistry analyzer. The precision of the method was good (within-run C.V. = 2.14%; between-run C.V. = 5.5%). A comparison of the new automated method with a manual and semi-automated method gave regression statistics of y = 1.18X -3.66 (Sy. x = 4.54), and y = 0.733X + 1.97 (Sy. x = 1.69), respectively. Temperature conversion factors provided by the kit manufacturer for human samples were determined to be inaccurate for converting results from rat samples. Analysis of components contributing to normal variation in rat serum 5'NT activity showed age and sex to be major factors. Increased serum 5'NT activity was observed in female rats when compared to male rats beginning at about 5 to 6 weeks of age. An analysis of variance of serum 5'NT, alkaline phosphatase, and GGT activities observed over a 9-week period in normal rats suggests several advantages for 5'NT as a predictor of biliary lesions in rats.  相似文献   

Physiologic races ofFusarium oxysporum f.sp.melonis in the southeastern anatolia region of turkey and varietal reactions to races of the pathogen     

Sener Kurt  B. Baran  N. Sarı  H. Yetisir 《Phytoparasitica》2002,30(4):395-402

Thirty-four isolates ofFusarium oxysporum f.sp.melonis (F.o.m.) obtained from 205 fields in melon-producing areas in the southeastern Anatolia Region of Turkey were identified on the basis of colony morphology and pathogenicity by the root dip method. In this region the mean prevalence of wilt disease was 88.1% and the mean incidence of disease was 47.5%. Physiologic races 0, 1, 2, and 1,2 of the pathogen were determined by their reactions on differential melon cultivars ‘Charentais T,’ ‘Isoblon’, ‘Isovac’ and ‘Margot’ in the greenhouse. Race 1,2, representating 58.8% (20/34) of all isolates, was widely distributed. Of the other pathogenic isolates, eight were identified as race 0, five as race 1, and one as race 2. This is the first report of physiologic races ofF.o.m. in Turkey. Of 44 melon cultivars tested in the greenhouse for resistance toF.o.m. races, 36 were found to be moderately resistant to race 0, 17 were susceptible to race 1,2, 34.1% were highly resistant to race 1, and 52.2% had moderate resistance to race 2. http://www.phytoparasitica.org posting July 16, 2002.  相似文献   

Determination of Energy Use Efficiency of some Apple (<Emphasis Type="Italic">Malus x domestica</Emphasis>) Production in Turkey: a Case Study of Eğirdir Region     

Osman Gokdogan  Mehmet Firat Baran 《Erwerbs-Obstbau》2017,59(1):13-18

This research aims to make an analysis of energy use efficiency of apple (‘Starkrimson Delicious’ and ‘Golden Delicious’) production in E?irdir region of Turkey, during the production season of 2012. Production data this research was collected in 2013. In order to determine the energy use efficiency of apple, various surveys have been conducted in 71 apple farms, selected through Simple Random Sampling method, located in E?irdir region of Turkey. The data have been collected through face to face questionnaires and first hand observations. The energy input and output values in apple production have been calculated as 34,703.63 MJ ha^?1 and 95,034 MJ ha^?1, respectively. Energy inputs consist of diesel fuel energy by 29.04?%, chemical fertilizers energy by 24.28?%, machinery energy by 15.70?%, chemical energy by 9.84?%, human labor energy by 8.54?%, electricity energy by 5.63?%, irrigation energy by 3.97?%, farmyard manure energy by 2.88?% and lime energy by 0.12?%. Energy use efficiency, energy productivity, specific energy and net energy in apple production have been calculated as 2.74, 1.16 kg MJ^?1, 0.86 MJ kg^?1 and 60,330.36 MJ ha^?1, respectively. The total energy input consumed has been classified as direct, by 47.17?%, indirect, by 52.83?%, renewable, by 15.38?% and non-renewable, by 74.62?%.  相似文献   

Determination of Energy Input-Output Analysis in Plum (<Emphasis Type="Italic">Prunus domestica</Emphasis> L.) Production     

Mehmet Firat Baran  Halil Ibrahim Oguz  Osman Gokdogan 《Erwerbs-Obstbau》2017,59(4):331-335

The aim of this research is to compose the energy input-output analysis of plum in Nevsehir province in Turkey. This research was conducted at the plum cultivating facilities during the 2015–2016 production seasons in Nevsehir province of Central Anatolian Region in Turkey. The agricultural input energies and output energies used in plum cultivation were calculated to determine the energy input-output analysis. According to the research findings, the energy inputs in plum cultivation were calculated respectively 3920?MJ ha^?1 (44.99%) chemical fertilizers energy, 1618.91?MJ ha^?1 (18.58%) diesel fuel energy, 1125.85?MJ ha^?1 (12.92%) chemicals energy, 1069.20?MJ ha^?1 (12.27%) machinery energy, 723.24?MJ ha^?1 (8.30%) human labour energy and 255?MJ ha^?1 (2.93%) irrigation water energy. Production output plum yield were calculated as 12,112.50?MJ ha^?1. The energy output/input ratio, specific energy, energy usage efficiency and net energy calculations were calculated respectively as 1.39, 1.37?MJ kg^?1, 0.73?kg MJ^?1 and 3400.30?MJ ha^?1.  相似文献   

Community water management to intensify agricultural productivity in the polders of the coastal zone of Bangladesh     

Yadav  Sudhir  Mondal  Manoranjan K.  Shew  Aaron  Jagadish  S. V. Krishna  Khan  Zahirul Haque  Sutradhar  Asish  Bhandari  Humnath  Humphreys  Elizabeth  Bhattacharya  Jayanta  Parvin  Rokhsana  Rahman  Mahabubur  Chandna  Parvesh 《Paddy and Water Environment》2020,18(2):331-343

Paddy and Water Environment - Most of the lands of the southern coastal zone of Bangladesh are protected from tidal flooding and storm surges by embankments constructed during the 1960s and 1970s,...  相似文献   

Effects of AG1 and AG2 QTLs on Nonstructural Carbohydrate and Seed Management Options for Rice Seedling Growth and Establishment under Flooding Stress     

Mondal Satyen  Jamil Hasan M.  Ahmed Tofayel  Giashuddin Miah M.  C. Sta Cruz Pompe  M. Ismail Abdel 《水稻科学》2020,27(6):515-528

Rice (Oryza sativa) plants acquired excess photosynthates in the form of nonstructural carbohydrates (NSCs) in their stems and grain. Despite keen interest in rice NSC, the dynamics of NSC accumulation, translocation and re-accumulation have not yet been well investigated. AG1 and AG2 QTLs associated with flooding tolerance through catalyzing starch into soluble sugar in germinating seeds. Here we conducted three experiments, greenhouse and field to lay the groundwork for large-scale diversity studies on grain NSC and some agronomic traits under direct-seeded rice (DSR) system, using elite lines incorporating AG1, AG2 and AG1-AG2 QTLs into the popular varieties PSB Rc82 and Ciherang-Sub1 along with the donors Kho Hlan On (AG1) and Ma-Zhan Red (AG2). In germinating seedlings, soluble sugars increased, while starch concentration decreased gradually especially in the tolerant checks and AG1-AG2 introgression lines under flooded soil. Soluble sugar accumulation in stem started to increase from the vegetative stage and peaked at the panicle initiation stage then gradually decreased towards the maturity stage. But Sub1-AG lines had higher sugar and starch concentrations at different growth stages than other genotypes in wet season 2016 and dry season 2017. Plant survival rate was positively correlated with the stem NSC at the early vegetative stage (21 days after sowing), and stem NSC was positively associated with plant height at different growth stages. Among the tested seeding rate, the most suitable seeding rate, 4 g/m² with shallow burial depth (0.5 cm), resulted in better seedling establishment, relatively higher seedling vigor index and higher leaf area index under flooding in DSR system. Introgression of AG1-AG2 QTLs had no any negative impact on nonstructural carbohydrate, germination rate, and growth and biomass production.  相似文献   

Effect of ground water high in Mg on cation exchange of Na-illite in the presence of CaCO₃     

R.C. Mondal 《Geoderma》1973,9(1):35-41

Ground waters with electrical conductivity of 4.9–7.4 mmhos/cm, a pH of 7.7–8.8 and a Mg/Ca ratio of 0.8–41.5 were equilibrated with a Na-illite in the presence and absence of CaCO₃. In the presence of CaCO₃, exchangeable Ca, exchangeable $\text{Ca+Mg}$ and the exchangeable Ca/Mg of the equilibrated clay increased but exchangeable Mg and Na decreased. The pH values of the ground waters were positively correlated with (1) exchangeable Ca, (2) exchangeable $\text{Ca+Mg}$, and (3) exchangeable Ca/Mg ratio, both in the presence and absence of CaCO₃.  相似文献   

The relationship between fermentation and the morphological structure of rumen epithelium in sheep fed pelleted feeds]     

M Baran  J Marcaník  J Kocisová  I Zelenák  M Belák 《Veterinární medicína》1979,24(10):587-595

Wethers were fed complete granular feed rations including 41.81% of grass hay, 25.28% of barley, 15.37% of sawdust, 14.98% of molasses, 1.32% of urea, and 1.24% of mineral supplement in dry matter for 24 weeks. Samples of the dorsal rumen sac of these wethers were subjected to patho-anatomical, histological, histo-chemical, and electron-microscopical examination. Volatile fatty acids were also determined in the rumen fluid of slaughtered animals. The control group was given the same diet in the classical form with long hay. The rumen contents of the slaughtered animals of the experimental group had an increased level of total volatile fatty acids (125.93 mM) and butyric acid (17.8 M%). The acetate:propionate ratio was 3.66. No substantial differences were observed in enzymatic activity. Electronograms recorded an increase in the number of T cells and keratinizing cells -- this suggests an increased intensity of the process of keratinization.  相似文献   

Reduction and stabilization of hip luxation by the transposition of the ligamentum sacrotuberale in dogs: an in vivo study     

Ozaydin I  Kiliç E  Baran V  Demirkan I  Kamiloglu A  Vural S 《Veterinary surgery : VS》2003,32(1):46-51

OBJECTIVES: To evaluate the effectiveness of transposition of the sacrotuberous ligament (LST) for the treatment of coxofemoral luxation in the dog. STUDY DESIGN: In vivo experimental study. SAMPLE POPULATION: Ten mixed-breed dogs (weighing 12 to 26 kg). METHODS: After general anesthesia, the LST was exposed and released from the sacrum with a sacral bone fragment. Coxofemoral luxation was created by capsulotomy and transection of the ligament of the head of the femur. Suture was passed through 2 small holes in the bone fragment to guide transposition of the LST through a tunnel drilled through the acetabulum and femoral head and neck. The suture material was pulled tight and an interference screw was placed into the femoral tunnel to lock the sacral bone fragment and LST securely in the tunnel. Butorphanol was administered for pain relief and dogs were allowed unrestricted activity. Coxofemoral radiographs were obtained at 15-day intervals. Two dogs were euthanatized for macroscopic and histopathologic examinations at 3 months. RESULTS: Release of the sacral bone was moderately difficult. The transposed ligament and bone fragment were of an appropriate length and were easily and securely fixed by a interference screw method. Visible severe lameness, during the first 7 to 10 days improved to mild lameness within 10 to 21 days. Gait was subjectively normal after 5 weeks. No radiographic abnormalities were observed at any time point. Grossly, the LST was intact and apparently viable and functional. On histopathology, the space in the bone tunnel was filled with new tissue and a hyaline-like layer surrounded the LST. The sacral bone fragment attached to the LST was united with the femoral bone. CONCLUSIONS: Transposition of the LST reduced and stabilized experimentally induced coxofemoral luxation in 10 dogs. Survival of the LST up to 3 months and its adaptation to transposition suggest that this surgical technique could be considered as a permanent treatment option for hip luxations. Transposition and fixation of the LST was easy, but releasing the sacral edge of the LST was difficult because of its deep location and division of the origin into 2 branches in some dogs. CLINICAL RELEVANCE: Results of this experimental study suggest that the technique may be satisfactorily used in dogs with coxofemoral luxation.  相似文献   

Genetic and antigenic diversity in avian infectious bronchitis virus isolates of the 1940s     

Jia W  Mondal SP  Naqi SA 《Avian diseases》2002,46(2):437-441

In order to verify a commonly held assumption that only Massachusetts (Mass) serotype of infectious bronchitis virus (IBV) was prevalent in the United States between the 1930s (when IBV was first isolated) and the 1950s (when the use of commercial IBV vaccines began), we examined 40 IBV field isolates from the 1940s. Thirty-eight of those isolates were recognized as Mass serotype viruses based on their reactivity to Mass-specific monoclonal antibody (Mab) and neutralization by Mass-specific chicken serum. The remaining two isolates, N-M24 and N-M39, that did not react with Mass-specific Mab, resisted neutralization by Mass-specific chicken serum, and were neutralized only by homologous chicken antibody were identified as non-Mass IBV. When the first 900 nucleotides (nt) from the 5'-end of the spike (S1) glycoprotein gene and their deduced amino acid (aa) sequences were compared, the two non-Mass isolates differed from each other by 24% and 28%, respectively. In a similar comparison, the non-Mass viruses N-M24 and N-M39 differed from M28, a Mass-type isolate from the 1940s, by 21% and 22% (nt) and 28% and 27% (aa), respectively. These data indicate that antigenic and genetic diversity among IBV isolates existed even in the 1940s. Interestingly, when the N-terminal region of the S1 of M28 was compared to that of M41, a prototype Mass virus that has undergone countless number of in vivo and in vitro host passages, the two viruses differed by only 2% (nt) and 4% (aa). This finding suggests that frequent genetic changes are not inherent in all IBV genomes.  相似文献