Miniaturized methods for testing the toxicity of pesticides to microorganisms     

S. L. COOPER  G. I. WINGFIELD  R. La WLEY  M. P GREAVES 《Weed Research》1978,18(2):105-107

A minialurized method of determining the toxicity of pesticides to large numbers or soil mitroorganisms is described. Bacteria. actinomycetes, yeasts, fungi and algae can be used as test organisms. A modification of the method allows determination of minimum inhibitory concentrations of pesticides to specific microorganisms. Some results obtained with herbicides are presented. Microméthodex pour éprauver la toxicité des pesticides vis-á-vis des micro-organismes Une micromethode pour determiner la toxicité des pesticides vis-á-vis de nombreux micro-organismes du sol est décrite. Des bactéries. des actinomycetes, des levures. des champignons et des algues peuvent ëtre utilisés comme organismes-tesis. Une modifitation de la méthode permel les determinations des concentrations minimales inhibitrices des pesticides vis-á-vis des micro-organismes specifiques. Quelques resultats obtenus avec des herbicides sont présentés. Miniaturisierte Methoden zur Prüf'ung der Toxizitüt von Pflan- zenhehandlumgsmit teln auf Mikraorganismen Es wird einc miniaturisierte Methode zur BestimmungderToxi- zität von Pfianzenbehandlungsmitteln für groβe Zablen von Bodenmikroorganismen beschrieben, Als Testorganismen können Bakterien. Actinomyceten. Hefen, Pilze und Algen benutzi werden. Eine Modifizierung der Methode gestattet die Bestimmung der Minimum-Hemmkonzentration von Pflanzen- behandlungsmitteln auf spezielle Mikroorganismen. Einige Ergebnisse, die mit Herbiziden erarbeitel wurden. sind darge-stellt.  相似文献   

Apoptosis in the Antral Follicles of Swamp Buffalo and Cattle Ovary: TUNEL and Caspase-3 Histochemistry     

JB Feranil  N Isobe  T Nakao 《Reproduction in domestic animals》2005,40(2):111-116

The present study was carried out to investigate the pattern of apoptosis in the healthy antral and atretic follicles of Philippine swamp buffaloes (BU) in comparison with Holstein-Friesian (HF) cows. Paraffin sections of healthy follicles and various stages of atretic follicles were stained using the terminal deoxynucleotidyl transferase (TdT)-mediated biotinylated deoxyuridine triphosphates (dUTP) nick end-labelling (TUNEL) method to detect DNA fragmentation and cleaved caspase-3 antibody to detect cells committed to undergo apoptosis. Five equidistant areas of a follicle were counted for the presence of TUNEL- and caspase-3-positive cells. Healthy follicles of BU and HF contained no TUNEL-positive cells in the granulosa and theca layer but showed some caspase-3 positivity. The granulosa layer of advanced atretic follicles showed a significantly higher frequency of caspase-3 positivity than the healthy and early atretic follicles in both breeds. The frequency of caspase-3-positive cells of BU was significantly higher than HF in the granulosa layer of healthy, early atretic and advanced atretic follicles. In the theca interna layer, BU and HF showed a significantly lower and higher frequency of TUNEL-positive cells in the late atretic follicles compared with advanced atretic follicle, respectively. However, the frequency of caspase-3-positive cells of both BU and HF in the late atretic follicles was significantly higher than the advanced atretic follicles in the theca interna layer. These results indicate that caspase-3 and DNA fragmentation is involved in the buffalo ovarian apoptotic process.  相似文献   

Cell Proliferation in the Atretic Follicles of Buffalo and Cattle Ovary     

JB Feranil  N Isobe  T Nakao 《Reproduction in domestic animals》2004,39(6):405-409

The present study was carried out to describe the proliferative activity of granulosa and theca cells in healthy antral and atretic follicles of Philippine buffaloes (BU) and Holstein-Friesian (HF) cows. Paraffin sections of ovary were immunostained with mouse monoclonal antibody to proliferating cell nuclear antigen (PCNA). Then the follicles were classified into healthy and various stages of atretic follicles. The granulosa layer of healthy follicles had a significantly higher frequency of PCNA-positive cells than the early and advanced atretic follicles in both breeds. In the theca interna, significantly reduced populations of the PCNA-positive cells were found in both breeds as atresia progressed. Moreover, HF had significantly higher PCNA-positive cells in the theca interna of healthy, early atretic and advanced atretic follicles than BU. A reduction of PCNA-positive cells during atresia was also noted in the theca externa in both animals although differences were not significant. The results of the present work suggest that the proliferative activity of granulosa and theca cells decreases in association with follicular atresia in the BU similar to HF. Furthermore, a significantly deficient cell proliferative activity of theca interna was found in BU compared with HF.  相似文献   

Viral diseases of the Ostrich (Struthio camelus var. domesticus)     

Ross Gordon COOPER  Jaroslaw Olav HORBANCZUK  Noboru FUJIHARA 《Animal Science Journal》2004,75(2):89-95

The ostrich is an important animal in many livestock industries. A significant threat to this industry is losses from diseases. Newcastle disease is a notifiable, highly contagious viral infection of ostriches. Avian influenza may be transmitted from waterfowl, shorebirds and gulls to ostriches. Borna disease virus is a viral neurotropic infection spread mainly by rodents and felines. Crimean‐Congo hemorrhagic fever is a viral disease transmitted by Hyalomma ticks to humans. Avipoxvirus afflicts ostrich chicks and is transmitted by mosquitoes or by direct contact with a pox lesion. Maintenance of a healthy and profitable enterprise requires the implementation, with assistance from the local veterinary authority, of comprehensive, practical and effective methods of health management and preventative medicine.  相似文献   

Ergovaline does not alter the severity of ryegrass staggers induced by lolitrem B     

SC Finch  JB Vlaming  BL Sutherland  C van Koten  WJ Mace  LR Fletcher 《New Zealand veterinary journal》2018,66(2):93-97

AIM: To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.

METHODS: Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with either AR98 endophyte (containing lolitrem B), standard endophyte (containing lolitrem B and ergovaline) or no endophyte, for up to 42 days from 2 February 2010. Ten lambs were grazed on three replicate plots per cultivar. Herbage samples were collected for alkaloid analysis and lambs were scored for ryegrass staggers (scores from 0–5) weekly during the study. Any animal which was scored ≥4 was removed from the study.

RESULTS: Concentrations of lolitrem B did not differ between AR98 and standard endophyte-infected pastures during the study period (p=0.26), and ergovaline was present only in standard endophyte pastures. Ryegrass staggers was observed in sheep grazing both the AR98 and standard endophyte plots, with median scores increasing in the third week of the study. Prior to the end of the 42-day grazing period, 22 and 17 animals were removed from the standard endophyte and AR98 plots, respectively, because their staggers scores were ≥4. The cumulative probability of lambs having scores ≥4 did not differ between animals grazing the two pasture types (p=0.41).

CONCLUSIONS AND CLINICAL RELEVANCE: There was no evidence for ergovaline increasing the severity of ryegrass staggers induced by lolitrem B. In situations where the severity of ryegrass staggers appears to be greater than that predicted on the basis of concentrations of lolitrem B, the presence of other tremorgenic alkaloids should be investigated.  相似文献   

A survey of the incidence of the Bromus species as weeds of winter cereals in England, Wales and parts of Scotland     

G. W. CUSSANS  F. B. COOPER  D. H. K. DAVIES†  M. R. THOMAS‡ 《Weed Research》1994,34(5):361-368

In June 1989, a survey was made of 733 fields of winter cereals, 627 in England and Wales and 106 in Fife and the Lothian regions of Scotland. The occurrence of Bromus species was noted in three areas of each field: the uncropped field margin, the cropped headland and the field centre. Three arbitrarily defined infestation levels were recorded: severe, light and intermediate. The most common species recorded was Bromus sterilis L., which accounted for almost 87% of all sightings. Most of the other occurrences were almost equally distributed between B. hordeaceus L., and B. commutatus Schrad. Two other species were found, on one field each: B. diandrus Roth and B. × pseudothominii Hard. Surveyors were briefed to recognize B. secalinus L, but none was reported. The brome grasses were widespread throughout the surveyed regions. They were most common in the Lothians (81 % of surveyed fields) and in the South East (60%) and the South West (52%) of England and least common in the English West Midlands, Fife and Wales (17%, 15% and 4% of surveyed fields). Distribution was biased towards the uncropped margins and field headlands. In England and Wales, there were 146 fields where Bromus species occurred in the field margins only, 179 in margins and headlands only and 63 with infestations in all three areas. In contrast, there were only 26 fields where they were restricted to the cropped areas. A similar pattern occurred in Scotland. The correlations between incidence of the Bromus species and management practices were much weaker than had been anticipated. The standard advice for control is to plough rather than use non-inversion tillage systems, to delay sowing and to avoid long runs of winter cereals. The conclusion of this survey is that, although these practices may be reducing or preventing increase of these weeds, they are not effective at eliminating infestations. Incidence des espèces Bromus comme mauvaises herbes des céréales d'hiver en Angleterre, au Pays de Galles et dans certaines parties de I'Écosse En juin 1989 une enquête a été réalisée dans 733 champs de céréales d'hiver, 627 en Angleterre et au Pays de Galles, et 106 dans les régions d'Écosse Fife et Lothian. La présence d'espèces Bromus a été notée sur trois zones de chaque champ: la bordure non-cultivée ('field margin'), la bordure cultivée ('headland') et le centre du champ. Trois niveaux d'infestation arbitraires ont eAté notés: sévère, léger et moyen. Les espèces les plus communément rencontrées étaient Bromus sterilis L. qui représentait presque 87 % de l'ensemble des présences. Dans la plupart des autres relevés, B. hordeaceus L. et B. commutatus Schrad se distribuaient presque également. Deuxautres espèces ont été trouvées, chacune dans un champ: B. diandrus Roth et B. × pseudothominii Hard. On avait appris aux enquêteurs à reconnaître B. secalinus L. mais cette espèce n'a pas été rapportée. Les bromes se rencontraient sur tout le territoire des régions étudiées. Elles étaient les plus communes dans les Lothians (81 % des champs étudiés) ainsi que dans le Sud Est (60 %) et dans le Sud Ouest (52 %) de l'Angleterre; les moins communes dans les West Midlands, en Fife et au Pays de Galles (17, 15 et 4 % des champs examinés). Leur distribution était biaisée en faveur des bordures non-cultivées et des bordures cultivées. En Angleterre et au Pays de Galles, dans 146 champs les bromes ne se rencontraient que dans les bordures non-cultivées, dans 179 ils ne se rencontraient que dans les bordures cultivées et non-cultivées, et dans 63 ils se rencontraient dans les trois secteurs du champ. Inversement, il n'y avait que 26 champs oü ils ne se trouvaient que dans la partie cultivée. Une répartition semblable s'observait en Écosse. Les corrélations entre la présence de bromes et la conduite des cultures étaient plus faibles qu'anticipé. Le conseil habituel est de labourer plutôt que de pratiquer des façons culturales sans renversement de la terre, de retarder le semis et d'éviter les longues successions de céréales d'hiver. La conclusion de cette étude est que, bien que ces pratiques peuvent réduire ou prévenir la prolifération de ces mauvaises herbes, elles ne sont pas efficaces pour les éliminer en cas d'infestations. Verbreitung von Bromus spp. in Wintergetreide in England, Wales und Teilen Schottlands Im Juni 1989 wurde auf 733 Wintergetreidefeldern, 627 in England und Wales und 106 in Fife und in den Lothian-Gebieten in Schottland, das Auftreten von Bromus spp. aufgenommen, getrennt nach dem nichtkultivierten Feldrand, dem Vorgewende und dem Feldinnern. Die Befallsstärke wurde nach schwer, mittel und leicht unterschieden. Die häufigste Trespen-Art war Bromus sterilis L. mit fast 87 % aller Beobachtungen. Die übrigen Vorkommen waren zu fast gleichen Teilen Bromus hordeaceus L. und Bromus commutatus Schrad. Aufje l Feld wurde Bromus diandrus Roth und Bromus × pseudothominii Hard, gefunden. Trotz intensiver Suche wurde kein Bromus secalinus L. festgestellt. Die Trespen traten im Beobachtungsgebiet verb-reitet auf, am häufigsten in den Lothians (81 % der untersuchten Felder) und im Südosten (60 %) und Südwesten (52 %) Englands, am wenigsten in dem englischen West Midland, in Fife und Wales (17, 15 bzw. 4 %). Die Verteilung konzentrierte sich in England und Wales auf 146 Feldern auf die kulturfreien Feldränder, in 179 Fällen auf die Feldränder sowie die Vorgewende, und auf 63 Feldern waren alle 3 Bereiche mit Trespen verunkrautet; nur auf 26 Feldern waren die Arten auf das Feldinnere beschränkt. In Schottland wurde eine ähnliche Verteilung beobachtet. Zwischen der Bewirtschaftung und dem Auftreten der Bromus spp. bestand eine weit schwächere Korrelation als erwartet. Die generelle Bekämpfungsempfehlung ist Pflügen statt nichtwendender Bodenbearbeitung, spätes Säen und Vermeidung von Fruchtfolgen mit hohem Wintergetreideanteil. Aus den Untersuchungsergebnissen folgt, daß diese Bekämp-fungsmaßnahmen Trespen wohl zurückdrängen oder ihre Zunahme verhindern, aber nicht eliminieren können.  相似文献   

Comparison of virulence of isolates of Fusarium oxysporum f.sp. elaeidis from Africa and South America     

J. FLOOD  R. MEPSTED  A. VELEZ  T. PAUL  R. M. COOPER 《Plant pathology》1993,42(2):168-171

The virulences of three isolates of Fusarium oxysporum f.sp. elaeidis from West Africa were compared with that of a Brazilian isolate. The Brazilian strain was more virulent and caused disease in all oil-palm lines tested, even those which had been selected for resistance to, and were generally unaffected by, African strains. Differential interactions between hosts and isolates of the pathogen could have serious implications for selection of breeding material and for the extension of oil-palm cultivation in South America.  相似文献   

Studies on blackleg bacterins. I. Immunity tests in laboratory animals     

PERSONEUS GR  COOPER MS  MARTINI FV 《The Cornell veterinarian》1957,47(3):361-371

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Comparative immunology     

Edwin L. COOPER 《Integrative zoology》2006,1(1):32-43

As a discipline, comparative immunology enhances zoology and has gained wide acceptance in the biological sciences. It is an offshoot of the parent field, immunology, and is an amalgam of immunology and zoology. All animals from protozoans to humans have solved the threat of extinction by having evolved an immune‐defense strategy that ensures the capacity to react against foreign, non‐self microorganisms and cancers that disturb the homeostatic self. Invertebrate‐type innate immune responses evolved first and they characterize the metazoans. These rapid natural responses act immediately and are often essential for the occurrence of slower, more specific, adaptive vertebrate‐type immune responses. As components of the innate immune system, there is an emphasis on several major steps in the evolutionary process: (i) recognition; (ii) the phagocytic cell; and (iii) the natural killer cell. When vertebrates evolved, beginning with fish, thymus‐controlled T cells first appeared, as did bone marrow‐derived B cells (first found in amphibians with long bones). These were the precursors of the plasma cells that synthesize and secrete antibodies. Confirming the concept of self/non‐self, invertebrates possess natural, non‐adaptive, innate, non‐clonal, non‐anticipatory immune responses, whereas vertebrates possess adaptive, acquired, clonal, and anticipatory responses. This symposium concerns: (i) aspects of the immune spectrum in representative groups; (ii) specific findings (in particular models; e.g. earthworms); (iii) clues as to the possible biomedical application of relevant molecules derived from animals, notably invertebrates; and (iv) some views on the more practical applications of understanding immune systems of invertebrates and ectotherms, and their possible role in survival.  相似文献   

Bovine mastitis   总被引：1，自引：0，他引：1  

McEWEN AD  COOPER MB 《The Veterinary record》1947,59(48):655-664

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