First Established Pregnancies in Mediterranean Italian Buffaloes (Bubalus bubalis) Following Deposition of Sexed Spermatozoa near the Utero Tubal Junction     

GA Presicce  S Verberckmoes  EM Senatore  P Klinc  D Rath 《Reproduction in domestic animals》2005,40(1):73-75

At the time of AI following Ovsynch protocol, a total of 51 buffaloes were randomly divided in a first group (n = 30) subjected to conventional AI into the uterine body with 20 million non-sex sorted frozen-thawed spermatozoa, while a second group (n = 21) was inseminated near the utero-tubal junction (UTJ) ipsilateral to the ovary carrying the preovulatory follicle with 2.5 million live (4 million total) sex-sorted frozen-thawed spermatozoa. The semen used for flowcytometric sorting was collected and processed on a farm in Italy, and then shipped to a laboratory in Germany. Eleven buffaloes were inseminated with X-chromosome bearing spermatozoa and 10 with Y-chromosome bearing spermatozoa. Conception rates after conventional and UTJ inseminations were 43.3% (n = 13) and 42.8% (n = 9) respectively (p = 0.97). Eight of the nine foetuses obtained after insemination with sexed spermatozoa corresponded to the sex as predicted by the cell sorting procedure (five male and four female foetuses by ultrasound vs six male and three female foetuses by cell sorting). In conclusion, for the first time buffalo semen has been successfully subjected to procedures for flowcytometric sperm sorting and freezing. Low doses of sexed spermatozoa have been deposited near the UTJ giving conception rates similar to those of conventional AI with full dose.  相似文献   

Retrospective evaluation of the use of small-bore wire-guided catheters for the management of peritoneal effusion in cats and dogs     

Jilli Crosby BVetMed  DVM  Karen Humm MA  VetMB  MSc  DACVECC  DECVECC  Simon D. Cook BSc  BVSc  MVetMed  DACVECC  DECVECC 《Journal of Veterinary Emergency and Critical Care》2023,33(1):91-97

Objective

To describe the use of small-bore wire-guided catheters in the management of peritoneal effusion in cats and dogs and to detail any associated adverse events.

Design

Retrospective study.

Setting

University teaching hospital

Animals

Forty-five client-owned animals that had peritoneal catheters placed for management of peritoneal effusion between July 2010 and June 2021.

Interventions

None.

Measurements and Main Results

Forty-five cases were included (25 dogs and 20 cats). Twenty-eight animals had the catheter placed to aid management of a uroabdomen, 8 of which recovered without surgical management, 11 had the catheter placed to allow autotransfusion of hemoabdomen, 3 had peritonitis, and 3 had ascites secondary to cardiac disease. Twenty-seven cases (15 dogs and 12 cats) received sedation (n = 24) or local anesthesia alone (n = 3) to facilitate catheter placement, and 6 cases had the catheter placed while under general anesthesia. Median length of catheter persistence was 24 hours (range: 2–144 h). The most common adverse events reported were impaired drainage (n = 7) and leakage at the insertion site (n = 4).

Conclusions

Peritoneal catheters can be inserted percutaneously for management of peritoneal effusion. Indications include stabilization and conservative management of uroabdomen, and autotransfusion. They can often be placed with minimal or no sedation and adverse events appear infrequent in occurrence.  相似文献   

Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs     

Robson RV Alves  Tatiana Soares  Elinaldo FL Bento  Ricardo S Roldan‐Filho  Brbara SS Souza  Marcele KN Lima  Jssica S Nascimento  Luana CBB Coelho  Roberto A S  Thmarah A Lima  Gabriel GA Gonalves  Fbio A Brayner  Luiz C Alves  Daniela MAF Navarro  Thiago H Napoleo  Patrícia MG Paiva 《Pest management science》2020,76(2):730-736

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关于开发建设项目水土保持监督管理体系的思考与建议     

高远  任明 《中国水土保持》2007,(3):31-32

把依法行政分解为法律基础和行政体系2个方面,分析了开发建设项目水土保持监督管理体系在法律基础和行政体系2个方面存在的问题。法律基础方面存在的问题:一是相关法律中提到开发建设项目水土保持监督管理的内容较少,各项职责落实不到位;二是法律中没有明确流域机构的水土保持职能。行政体系方面存在的问题有:规范化建设还不够完善,个别地方水保部门对监督管理工作内容不了解,权大于法的情况时有出现。提出了完善开发建设项目水土保持监督管理体系的建议。  相似文献   

防病促生枯萎病拮抗菌“98-Ⅰ”对4种枯萎病防治效果研究   总被引：3，自引：0，他引：3  

马利平  乔雄梧  高芬  郝变青 《中国生态农业学报》2005,(1)

经 8年系统试验研究从 330个细菌菌株中筛选出 1株防病促生枯萎病拮抗菌“98 Ⅰ” ,经鉴定属蜡质芽孢杆菌。该菌对黄瓜枯萎病、西瓜枯萎病、青椒枯萎病和番茄枯萎病 4种土传病害均有显著防治效果 ,其平皿孢子萌发抑制率分别为 79 2 %、75 1%、72 3%和 95 7% ,且该菌对多种蔬菜有促生和促进种子发芽功效。  相似文献   

大豆水溶性多糖的黏度性质及其应用研究     

梁建忠顾苒  高红亮孙敏 常忠义 《华北农学报》2007,22(B10):143-145

研究了不同条件下大豆水溶性多糖溶液的黏度变化情况，同时和其他常用的稳定剂如果胶等也做了比较。试验结果表明。本实验室生产的大豆水溶性多糖的水溶液的黏度随着温度的升高、浓度的降低、外加盐量的增加而降低；随着外加蔗糖量、pH值的增加而增加；相比较于其他稳定剂大豆水溶性多糖的黏度很低，使酸乳饮料具有清爽的口味。  相似文献   

Photolysis of chlorophenylacetic acids     

Crosby DG  Leitis E 《Journal of agricultural and food chemistry》1969,17(5):1036-1040

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Congenital arthrogryposis: an inherited limb deformity in pedigree Suffolk lambs     

Doherty ML  Kelley EP  Healy AM  Callanan JJ  Crosby TF  Skelly C  Boland MP 《The Veterinary record》2000,146(26):748-753

Thirty of 52 pedigree Suffolk lambs (58 per cent) were born with arthrogryposis characterised by bilateral flexion rigidity of the metacarpophalangeal and carpal joints. The recent introduction of a breeding ram was identified as the only significant risk factor in the flock, and embryo transfer was used to test the hypothesis that the arthrogryposis was an inherited disorder associated with the introduction of this ram. Two adult ewes that had previously produced lambs with arthrogryposis by the ram and four of its affected daughters were available as donors, and 20 crossbred ewes were used as recipients. Ten Suffolk-crossed ewes that had no known familial relationship with the ram were also mated by the ram as controls and they produced 10 normal lambs. Following embryo transfer, 19 lambs were born, of which seven were stillborn; arthrogryposis was evident in 10 of the 12 live lambs. Analysis of the data suggested that in the population under study, arthrogryposis was inherited as an autosomal recessive condition.  相似文献   

Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats     

HH Ortega  NR Salvetti  C Baravalle  JA Lorente  GA Mira 《Reproduction in domestic animals》2006,41(3):204-209

The objectives of this work were to determine the changes in the expression of neuroendocrine markers in Leydig cell by oestradiol treatment, and to determine whether testosterone is able to recover partially the effects of hormonal suppression induced by oestradiol. Adult male rats were injected daily with either 50 microg of oestradiol or oestradiol plus testosterone propionate (25 mg every 3 days) for 15 days. The animals were sacrificed and testicles were dissected and processed by routine histological protocols. FSH and LH serum levels were determined by radioimmunoassay. The visualization of antigens was achieved by the streptavidin-peroxidase immunohistochemical method. Antibodies against chromogranin A (CrA), S-100 protein (S-100), P substance (PS), synaptofisin (SYN), neurofilament protein (NF), gliofibrillary acidic protein (GFAP) and neuron specific enolase (NSE) were used. The mean LH and FSH serum concentrations were consistently suppressed with hormonal treatments. Intermediate filaments (NF and GFAP) showed no difference in their expression. The expression of S-100, NSE and SYN was significantly lower in both hormone-treated groups. In oestradiol-treated rats, the immunoreactivity of CrA and SP decreased significantly but was restored after testosterone supplementation. Although the nature and functions of many of these substances in Leydig cells remain unknown, these results are consistent with the hypothesis that the expression of some neuroendocrine markers is hormonally controlled.  相似文献   

The effects of plastic slatted floor or straw bedding on performance, liver weight and liver copper concentrations in intensively reared lambs     

J.P. Day  T.M. Boland  T.F. Crosby   《Livestock Science》2006,100(2-3):270-275

One hundred and eight Texel sired lambs were weaned at 37 days old and allocated three days later to one of two treatments, using two pens per treatment, in order to evaluate lamb hepatic copper (Cu) values. In Treatment 1 (T1) the lambs were confined to pens having recycled plastic slatted flooring, while in Treatment 2 (T2) the lambs were confined to pens having straw bedded floors, with fresh straw added on alternate days. The lambs had ad libitum access to a coarse lamb concentrate. The crude protein (g/kg DM), crude fibre (g/kg DM), total zinc (mg/kg) and background levels of copper (Cu) (mg/kg) and molybdenum (Mo) (mg/kg) of the diet were 17.2, 10.9, 152, 9.7 and 0.92, respectively. The lambs were slaughtered when they reached about 39 kg live weight. Following slaughter, the liver was removed and weighed and the caudal lobe frozen for subsequent Cu analysis. The livers of lambs on the plastic floor treatment had higher liver weights (843 vs. 804 g, S.E.M. 11.0; P < 0.05) and higher hepatic copper (396 vs. 315 mg/kg DM, S.E.M. 18.2; P < 0.05) than those from lambs that were bedded with straw. The Cu accumulation per kg of liver DM per week was estimated to be 8.4 and 13.7 mg for lambs on the straw bedding and plastic slated treatments, respectively. It is concluded that when lambs are housed on plastic slatted flooring, relative to straw bedding, there is a higher accumulation of hepatic copper levels likely to result in a greater risk of Cu toxicity when lambs are reared on all concentrate diets.  相似文献