Wild dogs in the serengeti     

East ML  Hofer H 《Science (New York, N.Y.)》1996,271(5247):275b-276b

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First Established Pregnancies in Mediterranean Italian Buffaloes (Bubalus bubalis) Following Deposition of Sexed Spermatozoa near the Utero Tubal Junction     

GA Presicce  S Verberckmoes  EM Senatore  P Klinc  D Rath 《Reproduction in domestic animals》2005,40(1):73-75

At the time of AI following Ovsynch protocol, a total of 51 buffaloes were randomly divided in a first group (n = 30) subjected to conventional AI into the uterine body with 20 million non-sex sorted frozen-thawed spermatozoa, while a second group (n = 21) was inseminated near the utero-tubal junction (UTJ) ipsilateral to the ovary carrying the preovulatory follicle with 2.5 million live (4 million total) sex-sorted frozen-thawed spermatozoa. The semen used for flowcytometric sorting was collected and processed on a farm in Italy, and then shipped to a laboratory in Germany. Eleven buffaloes were inseminated with X-chromosome bearing spermatozoa and 10 with Y-chromosome bearing spermatozoa. Conception rates after conventional and UTJ inseminations were 43.3% (n = 13) and 42.8% (n = 9) respectively (p = 0.97). Eight of the nine foetuses obtained after insemination with sexed spermatozoa corresponded to the sex as predicted by the cell sorting procedure (five male and four female foetuses by ultrasound vs six male and three female foetuses by cell sorting). In conclusion, for the first time buffalo semen has been successfully subjected to procedures for flowcytometric sperm sorting and freezing. Low doses of sexed spermatozoa have been deposited near the UTJ giving conception rates similar to those of conventional AI with full dose.  相似文献   

Interactions of insecticides with biological membranes     

Anthony G. Lee  J. Malcolm East  Paul Balgavy 《Pest management science》1991,32(3):317-327

Many insecticides (e.g. DDT, lindane, pyrethroids) are hydrophobic molecules which bind extensively to biological membranes. Binding of insecticides to phospholipid bilayers frequently shows saturation, with binding increasing with increasing insecticide concentration up to some limit, beyond which no further insecticide is incorporated into the bilayer. For lindane, this limit has been shown to correspond to the concentration at which the aqueous phase becomes saturated. Simple alkanes show similar saturation phenomena, and binding of halogenated alkanes can be followed by fluorescence quenching methods. It is shown that effects of hydrophobic molecules on bilayer fluidity are small. Effects of alkanes and insecticides on the activity of the (Ca²⁺-Mg²⁺)-ATPase purified from sarcoplasmic reticulum follow from direct binding to the ATPase, at sites which are not at the lipid-protein interface of the ATPase.  相似文献   

Corrigendum to "A DNA vaccine coding for glycoprotein B of pseudorabies virus induces cell-mediated immunity in pigs and reduces virus excretion early after infection"     

van Rooij EM  Haagmans BL  Glansbeek HL  de Visser YE  de Bruin MG  Boersma W  Bianchi AT 《Veterinary immunology and immunopathology》2000,75(1-2):161-162

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THE HARVARD BOTANICAL GARDEN IN CUBA     

East EM 《Science (New York, N.Y.)》1924,59(1533):433-434

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Cattle movement patterns in Australia: an analysis of the NLIS database 2008–2012          下载免费PDF全文

RM Iglesias  IJ East 《Australian veterinary journal》2015,93(11):394-403

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Spatial and temporal evaluation of veterinarians and veterinary employers relative to human and domesticated animal populations in Australia 2002–2012          下载免费PDF全文

GB Smyth  IJ East  RM Wicks 《Australian veterinary journal》2015,93(5):137-144

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Impact of foot-and-mouth disease on mastitis and culling on a large-scale dairy farm in Kenya     

Nicholas A Lyons  Neal Alexander  Katharina DC St?rk  Thomas D Dulu  Jonathan Rushton  Paul EM Fine 《Veterinary research》2015,46(1)

Foot and mouth disease (FMD) is a highly transmissible viral infection of cloven hooved animals associated with severe economic losses when introduced into FMD-free countries. Information on the impact of the disease in FMDV-endemic countries is poorly characterised yet essential for the prioritisation of scarce resources for disease control programmes. A FMD (virus serotype SAT2) outbreak on a large-scale dairy farm in Nakuru County, Kenya provided an opportunity to evaluate the impact of FMD on clinical mastitis and culling rate. A cohort approach followed animals over a 12-month period after the commencement of the outbreak. For culling, all animals were included; for mastitis, those over 18 months of age. FMD was recorded in 400/644 cattle over a 29-day period. During the follow-up period 76 animals were culled or died whilst in the over 18 month old cohort 63 developed clinical mastitis. Hazard ratios (HR) were generated using Cox regression accounting for non-proportional hazards by inclusion of time-varying effects. Univariable analysis showed FMD cases were culled sooner but there was no effect on clinical mastitis. After adjusting for possible confounders and inclusion of time-varying effects there was weak evidence to support an effect of FMD on culling (HR = 1.7, 95% confidence intervals [CI] 0.88-3.1, P = 0.12). For mastitis, there was stronger evidence of an increased rate in the first month after the onset of the outbreak (HR = 2.9, 95%CI 0.97-8.9, P = 0.057).

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0173-4) contains supplementary material, which is available to authorized users.  相似文献   

Emergency disease planning and preparedness     

Iain East 《Australian veterinary journal》2014,92(3):50-50

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长江口潮滩植物根际沉积物磷的累积及其生物有效性   总被引：3，自引：0，他引：3  

欧冬妮  刘敏  侯立军  刘巧梅  黄玥 《土壤通报》2004,35(3):290-294

通过对长江口潮滩植物生物量及根际沉积物中磷的赋存形态和含量的研究,结果表明各个采样点的海三棱草从春季开始生长并一直延续到初夏,到7月其生物量基本上都达到一个峰值。海三棱草根际沉积物中磷的赋存形态包括弱吸附态磷(Ad-P)、铁结合态磷(Fe-P)、自生磷灰石及钙结合态磷[(Ap+Ca)-P]、碎屑态磷(De-P)和有机磷(OP),它们都具有较好的季节性变化,在一定程度上都受到氧化还原状态的控制。文中还研究了根际沉积物孔隙水中的磷酸盐含量,发现其有效性受到了氧化还原状态及沉积物中铁的影响。  相似文献