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排序方式: 共有102条查询结果,搜索用时 36 毫秒
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Antoine LF Gady Freddy WK Hermans Marion HBJ Van de Wal Eibertus N van Loo Richard GF Visser Christian WB Bachem 《Plant methods》2009,5(1):13-14
Background
The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as a model for berry-fruit plants. With the progress of the tomato sequencing project, reverse genetics becomes an obvious and achievable goal. 相似文献3.
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AM Batista WA Gomes CCD Carvalho PLJ Monteiro Jr FLM Silva FC Almeida PC Soares GF Carneiro MMP Guerra 《Reproduction in domestic animals》2014,49(3):476-480
The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle‐stimulating hormone (FSH) i.m. in six descending doses at 12‐h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate‐buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick‐end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E2) and progesterone (P4) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p < 0.005), fewer embryos classified as degenerated (p < 0.001) and fewer TUNEL‐positive cells/blastocyst (p < 0.001). The number of transferable embryos was positively correlated with E2 concentrations on day of oestrus (r = 0.562; p < 0.01) and P4 concentrations on the day of embryo collection (r = 0.912; p < 0.001). We concluded that in vivo leptin administration during FSH treatment improved embryo quality and affected ovarian steroidogenesis in superovulated goats. 相似文献
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Orth CJ Gilmore JS Knight JD Pillmore CL Tschudy RH Fassett JE 《Science (New York, N.Y.)》1981,214(4527):1341-1343
An iridium abundance anomaly, with concentrations up to 5000 parts per trillion over a background level of 4 to 20 parts per trillion, has been located in sedimentary rocks laid down under freshwater swamp conditions in the Raton Basin of northeastern New Mexico. The anomaly occurs at the base of a coal bed, at the same stratigraphic position at which several well-known species of Cretaceous-age pollen became extinct. 相似文献
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Smartt SJ Maund JR Hendry MA Tout CA Gilmore GF Mattila S Benn CR 《Science (New York, N.Y.)》2004,303(5657):499-503
We present the discovery of a red supergiant star that exploded as supernova 2003gd in the nearby spiral galaxy M74. The Hubble Space Telescope (HST) and the Gemini Telescope imaged this galaxy 6 to 9 months before the supernova explosion, and subsequent HST images confirm the positional coincidence of the supernova with a single resolved star that is a red supergiant of 8(+4)(-2) solar masses. This confirms both stellar evolution models and supernova theories predicting that cool red supergiants are the immediate progenitor stars of type II-plateau supernovae. 相似文献
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Pillmore CL Tschudy RH Orth CJ Gilmore JS Knight JD 《Science (New York, N.Y.)》1984,223(4641):1180-1183
Iridium concentrations are anomalously high at the palynological Cretaceous-Tertiary boundary in fluvial sedimentary rocks of the lower part of the Raton Formation at several localities in the Raton Basin of New Mexico and Colorado. The iridium anomaly is associated with a thin bed of kaolinitic claystone in a discontinuous carbonaceous shale and coal sequence. 相似文献