Requirement for MHC class II positive accessory cells in an antigen specific bovine T cell response   总被引：1，自引：0，他引：1  

E J Glass  R L Spooner 《Research in veterinary science》1989,46(2):196-201

Purified populations of bovine antigen presenting cells (APCs) and T cells have been isolated from peripheral blood and characterised using various monoclonal antibodies (mAbs) for cell surface markers. Bovine APCs were found in an adherent cell fraction and were non-specific esterase positive, phagocytic and expressed bovine major histocompatibility complex (MHC) class II determinants, all of which are typical macrophage characteristics. T cells were rigorously depleted of accessory cell function before being used in an antigen presenting cell assay. The generation of T helper cells in response to the soluble antigen, ovalbumin, was entirely dependent upon a critical number of APCs. Further the proliferative response was inhibited by several mAbs to bovine MHC class II molecules. Thus the interaction between bovine APCs and helper/inducer T lymphocytes (TH/I) appears to be similar to that in other species.  相似文献   

Bronchoalveolar lavage in horses: effect of exercise and repeated sampling on cytology     

CK CLARK  GD LESTER  T. VETRO  B. RICE 《Australian veterinary journal》1995,72(7):249-252

SUMMARY Bronchoalveolar lavage (BAL) was performed at weekly intervals in 10 Thoroughbred horses in race training (group 1) and in 4 rested horses (group 2) for 10 weeks. Lavages were continued on a weekly basis in 4 group 1 horses for an additional 9 weeks (group 3). Cytological analysis of samples included leukocyte counts, erythrocyte counts, differential leukocyte counts, and haemosiderophage score. The mean leukocyte concentration was significantly lower in group 1(92.1 ± 4.6 cells/μL) when compared with group 2 (133.5 ± 8.2 cells/μL), P = 0.037. The differential leukocyte data were not significantly different between groups. There was a large amount of variability in the percent-age of macrophages and lymphocytes in the differential counts over time with no obvious trends. The proportion of neutrophils demonstrated a tendency to decrease over time for both groups 1 and 2. Erythrocyte counts and haemosiderin scores were significantly higher in the exercising group than the rested horses. Neither exercise nor the technique itself evoked an inflammatory response in the BAL fluid.  相似文献   

Lunar sample 14425: corrected analysis     

Glass BP  O'keefe JA 《Science (New York, N.Y.)》1985,229(4720):1410

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Atmospheric deposition     

Glass NR 《Science (New York, N.Y.)》1980,209(4454):384-385

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Influence of caudal epidural analgesia on cortisol concentrations and pain‐related behavioral responses in mares during and after ovariectomy via colpotomy          下载免费PDF全文

Aileen L. Rowland DVM  Kati G. Glass DVM  DACVS  Sicilia T. Grady DVM  PhD  Kevin J. Cummings DVM  PhD  Katrin Hinrichs DVM  PhD  DACT  Ashlee E. Watts DVM  PhD  DACVS 《Veterinary surgery : VS》2018,47(5):715-721

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Percutaneous absorption of chemicals: developing an understanding for the treatment of disease in frogs          下载免费PDF全文

V. K. Llewelyn  L. Berger  B. D. Glass 《Journal of veterinary pharmacology and therapeutics》2016,39(2):109-121

The permeable nature of frog skin presents an alternative route for the delivery of therapeutic chemicals to treat disease in frogs. However, although therapeutic chemicals are often topically applied to the skin of frogs, their pharmacokinetics have rarely been reported. To provide evidence to guide both candidate drug and formulation selection, we highlight factors expected to influence percutaneous absorption through frog skin, including the anatomy and physiology of the skin and the physicochemical properties of applied therapeutic chemicals. Importantly, we also highlight the effects of the formulation on percutaneous absorption, especially the inclusion of potential penetration enhancers as excipients. Finally, we collate empirical data on the topical application of various therapeutic chemicals in postmetamorphic frogs and show that, in contrast to mammalian species, even large chemicals (i.e. >500 Da) and those with a wide range of log P values (?4 through +6) are likely to be absorbed percutaneously. Topical application in frogs thus promises a convenient and effective method for delivering systemic treatments of a diverse range of chemicals; however, further experimental quantification is required to ensure optimal outcomes.  相似文献   

Characterization of house dust mite and scabies mite allergens by use of canine serum antibodies     

Schumann RJ  Morgan MS  Glass R  Arlian LG 《American journal of veterinary research》2001,62(9):1344-1348

OBJECTIVE: To identify the major allergenic proteins from the 3 main species of dust mites to which dogs react (Dermatophagoides farinae, D. pteronyssinus, and Euroglyphus maynei) and evaluate the potential cross-reactivity of dust mite allergens with antigens from the ectoparasitic mite Sarcoptes scabiei var canis. SAMPLE POPULATION: Sera from 83 dogs with atopic dermatitis. PROCEDURE: Sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting using serum from atopic dogs was used to identify IgE-binding proteins in extracts of the 4 mite species. RESULTS: Sera of atopic dogs contained IgE against 23, 17, 25, and 17 allergens from D. farinae, D. pteronyssinus, E. maynei, and S. scabiei, respectively. Unlike the situation for humans, the major allergens for dogs are mostly proteins that are larger than 90 kd molecular weight. Dermatophagoides farinae and E. maynei appear to be more allergenic for dogs than is D. pteronyssinus. Some dogs with serum IgE against dust mites also had IgE against antigens of S. scabiei var canis. CONCLUSIONS AND CLINICAL RELEVANCE: Multiple dust mite allergens induce an IgE response in dogs. These allergens are mostly greater than 90 kd molecular weight.  相似文献   

A modified bilateral transfrontal sinus approach to the canine frontal lobe and olfactory bulb: surgical technique and five cases     

Glass EN  Kapatkin A  Vite C  Steinberg SA 《Journal of the American Animal Hospital Association》2000,36(1):43-50

Five adult dogs presented for an acute onset of seizure activity. Magnetic resonance imaging revealed lesions in the olfactory bulbs, frontal lobes of the cerebrum, or both. A modified bilateral transfrontal sinus craniotomy was performed on each patient. The goal of removing the lesion was to relieve clinical signs and to provide tissue for histopathological diagnosis. In each instance, excision of the lesion was possible using this approach. No postoperative complications were observed. The modified bilateral transfrontal sinus craniotomy provides excellent access to the canine olfactory bulbs and frontal lobes.  相似文献   

评估调质时间和温度对猪颗粒饲料淀粉糊化度和维生素沉积的影响     

Lewis LL  Stark CR  Fahrenholz AC  Bergstrom JR  Jones CK 《广东饲料》2015,24(5)

本论文研究了饲料加工的两个关键参数(调质温度和时间)对育肥猪颗粒饲料淀粉糊化度和维生素沉积的影响。日粮配方为含30%干酒糟及其可溶物的玉米-豆粕型基础日粮。整个试验中配方保持不变。本试验采用2×3双因子设计,调质温度分别为77℃和88℃,调质时间分别15秒、30秒和60秒。此外,本试验还设置一个对照组,对照组饲料不采用调质制粒工艺,而是采用粉料饲喂。因此,本试验共有7个处理组。采集调质后制粒前(热干粉)、制粒后冷却前(热制粒)、以及制粒冷却后(冷制粒)的样品,并分析这三种样品的总淀粉率、淀粉糊化  相似文献   

Changes in the fish species composition of all Austrian lakes >50 ha during the last 150 years     

D. ZICK  & H. GASSNER  P. FILZMOSER  J. WANZENBÖCK  B. PAMMINGER-LAHNSTEINER  & G. TISCHLER 《Fisheries Management and Ecology》2006,13(2):103-111

Abstract  The fish communities of all Austrian natural lakes ( n  = 43) larger than 50 ha in surface area were assessed and the historical fish communities in c . 1850 were reconstructed. During the last 150 years, the fish communities of Austrian lakes have altered: in 49% of lakes at least one indigenous fish species, usually sensitive or small-bodied taxa, is now missing. Conversely, in all but one of the 43 lakes the number of fish species has increased. In particular, certain fish species of interest to angling and commercial fisheries now occur in more lakes. Generalised linear models were used to identify variables that would explain the loss of fish species using categories of lake use (ranking score). The category human population density around the lakes seemed to be one of the main causes for the loss of fish species.  相似文献