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排序方式: 共有182条查询结果,搜索用时 503 毫秒
1.
Requirement for MHC class II positive accessory cells in an antigen specific bovine T cell response 总被引:1,自引:0,他引:1
Purified populations of bovine antigen presenting cells (APCs) and T cells have been isolated from peripheral blood and characterised using various monoclonal antibodies (mAbs) for cell surface markers. Bovine APCs were found in an adherent cell fraction and were non-specific esterase positive, phagocytic and expressed bovine major histocompatibility complex (MHC) class II determinants, all of which are typical macrophage characteristics. T cells were rigorously depleted of accessory cell function before being used in an antigen presenting cell assay. The generation of T helper cells in response to the soluble antigen, ovalbumin, was entirely dependent upon a critical number of APCs. Further the proliferative response was inhibited by several mAbs to bovine MHC class II molecules. Thus the interaction between bovine APCs and helper/inducer T lymphocytes (TH/I) appears to be similar to that in other species. 相似文献
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Influence of caudal epidural analgesia on cortisol concentrations and pain‐related behavioral responses in mares during and after ovariectomy via colpotomy 下载免费PDF全文
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Percutaneous absorption of chemicals: developing an understanding for the treatment of disease in frogs 下载免费PDF全文
V. K. Llewelyn L. Berger B. D. Glass 《Journal of veterinary pharmacology and therapeutics》2016,39(2):109-121
The permeable nature of frog skin presents an alternative route for the delivery of therapeutic chemicals to treat disease in frogs. However, although therapeutic chemicals are often topically applied to the skin of frogs, their pharmacokinetics have rarely been reported. To provide evidence to guide both candidate drug and formulation selection, we highlight factors expected to influence percutaneous absorption through frog skin, including the anatomy and physiology of the skin and the physicochemical properties of applied therapeutic chemicals. Importantly, we also highlight the effects of the formulation on percutaneous absorption, especially the inclusion of potential penetration enhancers as excipients. Finally, we collate empirical data on the topical application of various therapeutic chemicals in postmetamorphic frogs and show that, in contrast to mammalian species, even large chemicals (i.e. >500 Da) and those with a wide range of log P values (?4 through +6) are likely to be absorbed percutaneously. Topical application in frogs thus promises a convenient and effective method for delivering systemic treatments of a diverse range of chemicals; however, further experimental quantification is required to ensure optimal outcomes. 相似文献
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Schumann RJ Morgan MS Glass R Arlian LG 《American journal of veterinary research》2001,62(9):1344-1348
OBJECTIVE: To identify the major allergenic proteins from the 3 main species of dust mites to which dogs react (Dermatophagoides farinae, D. pteronyssinus, and Euroglyphus maynei) and evaluate the potential cross-reactivity of dust mite allergens with antigens from the ectoparasitic mite Sarcoptes scabiei var canis. SAMPLE POPULATION: Sera from 83 dogs with atopic dermatitis. PROCEDURE: Sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting using serum from atopic dogs was used to identify IgE-binding proteins in extracts of the 4 mite species. RESULTS: Sera of atopic dogs contained IgE against 23, 17, 25, and 17 allergens from D. farinae, D. pteronyssinus, E. maynei, and S. scabiei, respectively. Unlike the situation for humans, the major allergens for dogs are mostly proteins that are larger than 90 kd molecular weight. Dermatophagoides farinae and E. maynei appear to be more allergenic for dogs than is D. pteronyssinus. Some dogs with serum IgE against dust mites also had IgE against antigens of S. scabiei var canis. CONCLUSIONS AND CLINICAL RELEVANCE: Multiple dust mite allergens induce an IgE response in dogs. These allergens are mostly greater than 90 kd molecular weight. 相似文献
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Glass EN Kapatkin A Vite C Steinberg SA 《Journal of the American Animal Hospital Association》2000,36(1):43-50
Five adult dogs presented for an acute onset of seizure activity. Magnetic resonance imaging revealed lesions in the olfactory bulbs, frontal lobes of the cerebrum, or both. A modified bilateral transfrontal sinus craniotomy was performed on each patient. The goal of removing the lesion was to relieve clinical signs and to provide tissue for histopathological diagnosis. In each instance, excision of the lesion was possible using this approach. No postoperative complications were observed. The modified bilateral transfrontal sinus craniotomy provides excellent access to the canine olfactory bulbs and frontal lobes. 相似文献
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Abstract. The feasibility of including individual records on correlated traits in a family selection programme which aims to increase resistance to furunculosis in Atlantic salmon was studied; markers were selected because of their potential role in the pathogenesis of the disease. Fibrinogen and α2 -antiplasmin both show genetic variation; both are correlated with survival after challenge with Aeromonas salmonicida , the correlation being 0·44 and 0·37 ( P < 0·05), respectively, and it is possible to measure both on a large scale at low costs. Contrary to α2 -antiplasmin, fibrinogen was negatively correlated with survival due to furunculosis within the 10 most resistant families and within the 10 most susceptible families in contrast to an overall positive correlation. This inconsistency could be attributable to the presence of different allelic phases in different families, and of major linked loci influencing survival and fibrinogen levels. Thus, only α2 -antiplasmin fulfils the requirements for a marker trait for resistance to the disease suitable for individual selection at the population level, whereas the use of fibrinogen would be restricted to within family selection. The full statistical model explained 51% of the variation in resistance to furunculosis, and α2 -antiplasmin contributed 15% to this variation when considered as a separate entity. Thus, the additional gain from including individual records on α2 -antiplasmin in a family selection programme could be significant. 相似文献
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G. Cui C. S. Wardle C. W. Glass A. D. F. Johnstone W. R. Mojsiewicz 《Fisheries Research》1991,10(3-4):255-263
A continuously swimming school of 12 dark-adapted mackerel was challenged to show visual reaction to barriers made from horizontal parallel lines (5 cm apart) of either monofilament nylon (0.40 mm diameter) gillnet material or, for comparison, the more visible twisted multifilament nylon (1.73 mm diameter). Six colours of each material were tested. The barriers were placed across the path of the approaching mackerel school while their behaviour was observed at low intensities of green light arranged to mimic undersea conditions. At very low light levels, the mackerel swam without hesitation through the barrier, whereas at higher light levels they turned or paused, indicating that they visually aware of the presence of the lines. The light level thresholds for visual reaction for the six colours of monofilament nylon were between −0.06 and −1.0 log lx, and for the same colours of twisted multifilament nylon were between −1.8 and −3.6 log lx. These findings are discussed in relation to the natural intensities of light in the sea, and the prediction of the visibility of drift and gillnet materials. 相似文献
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