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Complement fixation and ELISA tests were carried out on 8772 bovine sera. Results showed that ELISA titres were, on average, approximately sixteen times higher than the corresponding C.F. titres. The specificity of ELISA appeared comparable to that of the C.F.T. There was no evidence to show that the ELISA could detect infection earlier than the C.F.T.  相似文献   
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Wu  Jianguo  Hobbs  Richard 《Landscape Ecology》2002,17(4):355-365
Landscape ecology has made tremendous progress in recent decades, but as a rapidly developing discipline it is faced with new problems and challenges. To identify the key issues and research priorities in landscape ecology, a special session entitled “Top 10 List for Landscape Ecology in the 21st Century” was organized at the 16th Annual Symposium of the US Regional Association of International Association of Landscape Ecology, held at Arizona State University (Tempe, Arizona, USA) during April 25–29, 2001. A group of leading landscape ecologists were invited to present their views. This paper is intended to be a synthesis, but not necessarily a consensus, of the special session. We have organized the diverse and wide-ranging perspectives into six general key issues and 10 priority research topics. The key issues are: (1) interdisciplinarity or transdisciplinarity, (2) integration between basic research and applications,(3) Conceptual and theoretical development, (4) education and training, (5)international scholarly communication and collaborations, and (6) outreach and communication with the public and decision makers. The top 10 research topics are: (1) ecological flows in landscape mosaics, (2) causes, processes, and consequences of land use and land cover change, (3) nonlinear dynamics and landscape complexity, (4) scaling, (5) methodological development, (6) relating landscape metrics to ecological processes, (7) integrating humans and their activities into landscape ecology, (8) optimization of landscape pattern, (9)landscape sustainability, and (10) data acquisition and accuracy assessment. We emphasize that, although this synthesis was based on the presentations at the“Top 10 List” session, it is not a document that has been agreed upon by each and every participant. Rather, we believe that it is reflective of the broad-scale vision of the collective as to where landscape ecology is now and where it may be going in future. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
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Objective To measure the prevalence of megabacteria in budgerigar-breeding colonies and to evaluate possible methods to reduce the prevalence.
Design A monitoring study over several years.
Sample population Two budgerigar ( Melopsittacus undulatus ) colonies with over 300 birds each.
Procedure The prevalence of megabacteria in the faeces in two budgerigar breeding colonies, colony 1 and 2, was determined by faecal examination of each bird. Following an initial survey (1990), most of the birds that were scored 2+ or more were culled and a management practice was implemented to discriminate against positive birds. Consecutive yearly surveys (1991, 1992) were conducted on the young birds bred in these colonies. The prevalence of megabacteria in colony 2 was also evaluated in 1994 and 1996 after all the birds were treated with amphotericin B administered in drinking water.
Results The prevalence of megabacteria in the two colonies was significantly (P < 0.001) different. Overall the prevalence of megabacteria adjusted for colony differences was significantly higher (P < 0.025) in males compared to females. Age was not an influencing factor. After the initial survey, the prevalence in the offspring did not significantly (P > 0.05) decrease in the following two annual breeding seasons but by inference it did significantly decrease after amphotericin B treatment.
Conclusion The practice of culling most birds with more megabacteria in faeces and discriminating against positive birds when selecting birds for breeding or culling birds on show quality does not decrease megabacteria prevalence in the offspring. However, a reduction in prevalence does occur with administration of amphotericin B. Birds may have amphotericin B-resistant organisms and these birds need to be identified and culled.  相似文献   
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Objective: To (1) identify prognostic indicators for stability after stabilization of sacroiliac luxation with screws inserted in lag fashion and (2) report dorsoventral dimensions of the sacrum in cats. Study Design: Multicenter retrospective study. Sample Population: Cats (n=40) with sacroiliac luxation. Methods: Case records and radiographs of cats presented at the Queen's Veterinary School Hospital Cambridge and the Royal Veterinary College Hatfield for screw fixation of sacroiliac luxation were reviewed. Dorsoventral dimensions of 15 feline cadaveric sacral bodies were measured to identify the appropriate implant size for use in fixation with screws inserted in lag fashion. Results: Of 40 cats, 13 had left, 14 right, and 13 bilateral sacroiliac luxations. Of 48 screws analyzed, 42 (87.5%) were placed within the sacral body or exited ventrally and 6 (12.5%) were considered malpositioned. Screw purchase within the sacrum was statistically different between unstable and stable repairs (P=.001). Using confidence intervals for screw length within the sacrum and effect on stability, the lowest screw depth that contained 95% of the screws that did not loosen was ∼60% of the sacral width. Mean dorsoventral sacral dimension at its narrowest point was 5.9±1.14 mm. There was no significant difference in the incidence of implant loosening between those luxations that were 100% reduced and those that were <100% reduced (P=.7837). Conclusions: Screw purchase within the feline sacrum of at least 60% of the sacral width significantly reduces the risk of loosening. Clinical Relevance: Screw placement to a depth of 60% of the width of the feline sacrum is recommended.  相似文献   
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