全文获取类型
收费全文 | 397篇 |
免费 | 37篇 |
国内免费 | 2篇 |
专业分类
林业 | 16篇 |
农学 | 9篇 |
37篇 | |
综合类 | 27篇 |
农作物 | 12篇 |
水产渔业 | 27篇 |
畜牧兽医 | 274篇 |
园艺 | 9篇 |
植物保护 | 25篇 |
出版年
2023年 | 6篇 |
2022年 | 6篇 |
2021年 | 16篇 |
2020年 | 24篇 |
2019年 | 13篇 |
2018年 | 24篇 |
2017年 | 19篇 |
2016年 | 20篇 |
2015年 | 10篇 |
2014年 | 25篇 |
2013年 | 34篇 |
2012年 | 18篇 |
2011年 | 22篇 |
2010年 | 19篇 |
2009年 | 19篇 |
2008年 | 10篇 |
2007年 | 14篇 |
2006年 | 10篇 |
2005年 | 8篇 |
2004年 | 16篇 |
2003年 | 13篇 |
2002年 | 7篇 |
2001年 | 4篇 |
2000年 | 9篇 |
1999年 | 2篇 |
1998年 | 15篇 |
1997年 | 10篇 |
1996年 | 11篇 |
1995年 | 10篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1977年 | 1篇 |
1948年 | 1篇 |
排序方式: 共有436条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
Flow cytometric sorting technology was used to measure the difference in DNA content between X- and Y-chromosome-bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X- and Y-chromosome-bearing sperm populations and to quantify the DNA difference between them. Two symmetrical, overlapping and clearly separated peaks, corresponding to X- and Y-bearing spermatozoa, were detected. The difference in fluorescence intensity between the peaks was 4.4 +/- 0.03% without any significant inter- or intra-animal variations. Therefore, the identification and selection of high-purity samples of sperm populations for sex sorting is easier in bucks compared with other domestic species. 相似文献
5.
DW Bartlett JM Clough JR Godwin AA Hall M Hamer B Parr‐Dobrzanski 《Pest management science》2004,60(3):309-309
The original article to which this Correction refers was published in Pest Management Science 58 (7): 649–662 (2002).Copyright © 2004 Society of Chemical Industry 相似文献
6.
Equine pituitary pars intermedia dysfunction: current understanding and recommendations from the Australian and New Zealand Equine Endocrine Group
下载免费PDF全文
![点击此处可从《Australian veterinary journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
CJ Secombe SR Bailey MA de Laat KJ Hughes AJ Stewart JM Sonis RHH Tan 《Australian veterinary journal》2018,96(7):233-242
The purpose of this article is to provide a review of the current knowledge and opinions about the epidemiology, clinical findings (including sequelae), diagnosis, treatment and monitoring of equine pituitary pars intermedia dysfunction, particularly in the Australian context. This information and the recommendations provided will assist practitioners in making informed decisions regarding the diagnosis and management of this disorder. 相似文献
7.
The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0–13 × 105 cells per flask, were challenged with SP from bulls of high or low fertility (n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr (n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high‐ or low‐fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high‐ or low‐fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose‐dependent and fertility‐dependent. 相似文献
8.
RN Zadoks E Scholz SM Rowe JM Norris HB Pooley J House 《Australian veterinary journal》2023,101(4):142-152
Numerous culture-based diagnostics are available on the Australian and international markets for on-farm detection of bacterial pathogens in milk. Use of such diagnostics may provide an opportunity to improve the prudent use of antimicrobials in udder health management. Farms are low-resource settings in terms of diagnostic microbiology capacity. The World Health Organisation has identified criteria for the evaluation of diagnostic tests in low resource settings based on Accuracy, Sensitivity, Specificity, User-friendliness, being Rapid or Robust, Equipment-free and being Deliverable (ASSURED). Here, we review how those criteria can be interpreted in the context of microbiological diagnosis of mastitis pathogens, and how on-farm diagnostics that are currently available in Australia perform relative to ASSURED criteria. This evaluation identifies multiple trade-offs, both with regard to scientific criteria and with regards to convenience criteria. More importantly, the purpose of testing may differ between farms, and test performance should be evaluated relative to its intended use. The ability of on-farm mastitis diagnostics to inform mastitis treatment decision-making in a timely and cost-effective manner depends not just on test characteristics but also on farm-specific pathogen prevalence, and on the farm enterprise's priorities and the farm manager's potential courses of action. With most assay evaluations to date conducted in professional laboratories, there is a surprising dearth of information on how well any of the diagnostic tests perform on-farm and, indeed, of the on-farm decision-making processes that they aim to inform. 相似文献
9.
Goyette Jean-Olivier Cimon-Morin Jérôme Mendes Poliana Thériault Maxime Pellerin Stéphanie Poulin Monique 《Landscape Ecology》2021,36(9):2691-2706
Landscape Ecology - Human activities have led to the degradation of wetlands, impinging on their capacity to deliver essential ecosystem services to society. Wetland restoration now appears an... 相似文献
10.
Chemical restraint of peccaries with tiletamine/zolazepam and xylazine or tiletamine/zolazepam and butorphanol 总被引:3,自引:0,他引:3
Selmi AL Mendes GM Figueiredo JP Guimarães FB Selmi GR Bernal FE McMannus C Paludo GR 《Veterinary anaesthesia and analgesia》2003,30(1):24-29
Objective To evaluate the use of a combination of tiletamine/zolazepam and xylazine (TZX) in collared and white‐lipped peccaries and to compare its efficacy as an anesthetic technique with that of tiletamine/zolazepam and butorphanol (TZB). Study design Prospective experimental trial. Animals Seven white‐lipped peccaries (Tayassu pecari) (four females and three males) and four collared peccaries (Tayasu tajacu) (two males and two females). Methods Animal immobilization was attempted with TZX and TZB (IM) on two different occasions. Heart and respiratory rates (HR, RR), rectal temperature (RT), sedation, muscle relaxation, posture, auditory response and analgesia were evaluated every 15 minutes during immobilization. Induction, anesthesia, standing and walking time were determined after drug administration. Results Doses for white‐lipped peccaries were 1.23 ± 0.26 mg kg?1 (mean ± SD) of TZ and 1.23 ± 0.26 mg kg?1 of X, and 1.46 ± 0.09 mg kg?1 of TZ and 0.14 ± 0.008 mg kg?1 of B; doses for collared peccaries were 1.51 ± 0.29 mg kg?1 of TZ and 1.51 ± 0.29 mg kg?1 of X and 1.68 ± 0.02 mg kg?1 of TZ and 0.17 ± 0.002 mg kg?1 of B. In white‐lipped peccaries, both drug combinations provided a smooth induction and good immobilization for more than an hour. Anesthesia and standing times were significantly longer in animals given TZB, whereas walking time was significantly longer in animals given TZX. A significant decrease in HR was observed with both treatments. Respiratory rate decreased significantly with TZX, but the rate remained higher than with TZB. Induction and recovery quality in white‐lipped peccaries was better with TZB than with TZX. Neither protocol provided adequate immobilization in collared peccaries. Conclusion and clinical relevance At the doses described, TZB is effective in providing a long period of immobilization, whereas TZX is adequate for short to medium immobilization in white‐lipped peccaries. Neither drug combination was effective in collared peccaries at the doses given. 相似文献