Soluble matrix of hen's eggshell extracts changes in vitro the rate of calcium carbonate precipitation and crystal morphology     

J. Gautron  M. Bain  S. Solomon  Y. Nys 《British poultry science》1996,37(4):853-866

1. Extra and intramineral eggshell matrix proteins were solubilised before and after demineralisation by sequential extractions using guanidine hydrochloride and EDTA.

2. The intramineral electrophoretic profile of SDS‐PAGE showed the presence of 80, 66, 43, 36 and 15 kDa bands with a predominance of a 17 kDa band. In the extramineral part, the major protein was the 15 kDa band.

3. The introduction of intramineral extract to a metastable solution of calcium carbonate delayed the rate of crystal growth. The delay in the rate of precipitation was elicited by a single fraction (MW 50–80 kDa), isolated by gel filtration chromatography, of eggshell extracts. Extramineral extracts had no effect.

4. Addition in vitro of intramineral eggshell extracts modified the morphology of calcite; the crystals aggregated and showed irregular surfaces.

5. These observations suggest that constituents of the eggshell matrix are involved in the control of calcite growth and crytallographic structure of the hen's eggshell.  相似文献   

Ergovaline does not alter the severity of ryegrass staggers induced by lolitrem B     

SC Finch  JB Vlaming  BL Sutherland  C van Koten  WJ Mace  LR Fletcher 《New Zealand veterinary journal》2018,66(2):93-97

AIM: To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.

METHODS: Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with either AR98 endophyte (containing lolitrem B), standard endophyte (containing lolitrem B and ergovaline) or no endophyte, for up to 42 days from 2 February 2010. Ten lambs were grazed on three replicate plots per cultivar. Herbage samples were collected for alkaloid analysis and lambs were scored for ryegrass staggers (scores from 0–5) weekly during the study. Any animal which was scored ≥4 was removed from the study.

RESULTS: Concentrations of lolitrem B did not differ between AR98 and standard endophyte-infected pastures during the study period (p=0.26), and ergovaline was present only in standard endophyte pastures. Ryegrass staggers was observed in sheep grazing both the AR98 and standard endophyte plots, with median scores increasing in the third week of the study. Prior to the end of the 42-day grazing period, 22 and 17 animals were removed from the standard endophyte and AR98 plots, respectively, because their staggers scores were ≥4. The cumulative probability of lambs having scores ≥4 did not differ between animals grazing the two pasture types (p=0.41).

CONCLUSIONS AND CLINICAL RELEVANCE: There was no evidence for ergovaline increasing the severity of ryegrass staggers induced by lolitrem B. In situations where the severity of ryegrass staggers appears to be greater than that predicted on the basis of concentrations of lolitrem B, the presence of other tremorgenic alkaloids should be investigated.  相似文献   

Myocarditis caused by naturally acquired canine distemper virus infection in 4 dogs     

Dae Young Kim  Michael M. Zinn  Solomon O. Odemuyiwa  William J. Mitchell  Jr.  Gayle C. Johnson 《Journal of veterinary diagnostic investigation》2021,33(1):167

Canine distemper virus (CDV) has long been recognized as a cause of myocarditis; however, cases of myocarditis caused by naturally acquired CDV infection have been reported only rarely in dogs. We describe here our retrospective study of naturally acquired systemic CDV infection in 4 dogs, 4–7 wk old, that had myocarditis, with myocardial necrosis and fibrosis. One of the 4 dogs had intracytoplasmic eosinophilic inclusion bodies in cardiomyocytes. Other lesions included bronchointerstitial pneumonia (4 of 4), necrotizing hepatitis (2 of 4), splenic lymphoid necrosis (2 of 4), encephalitis (1 of 3; brain was not submitted in 1 case), and necrotizing gastroenteritis (1 of 4). The presence of CDV in the heart was confirmed by immunohistochemistry in all 4 dogs.  相似文献   

Exploitation of predators in UK fruit and hop culture     

M. G. Solomon 《Phytoparasitica》1992,20(1):S51-S56

A wide range of species of predators has been exploited, or has the potential to be exploited, as biological control agents against pests of fruit and hop. They include members of the families Miridae, Anthocoridae and Chrysopidae, and of the mite family Phytoseiidae. Some species can colonise fruit plantations naturally, whereas others are mass-cultured and introduced artificially. The characteristics of the various predator species are considered in relation to the requirements for biological control agents in specific crop situations.  相似文献   

Transmission electron microscopy of the vertical crystal layer and cuticle of the eggshell of the domestic fowl     

Fraser AC  Bain MM  Solomon SE 《British poultry science》1999,40(5):626-631

1. Eggshell pieces (1 cm2) were decalcified using a solution of EDTA (200 g/litre, pH 6.9 to 7.0) in paraformaldehyde (2 g/litre) and glutaraldehyde (0.5 g/litre) in phosphate buffer. 2. They were prepared for transmission electron microscopy (TEM). 3. TEM identified a vertically aligned matrix associated with the vertical crystal layer (VCL). It is hypothesised that the vertical orientation of calcite crystals in the VCL is closely linked to this vertical matrix. 4. TEM also revealed the presence of a 2-layered cuticle, the inner layer containing vesicles which were absent in the outer. 5. Cuticular vesicles contain hydroxyapatite and are thought to play a role in the termination of shell formation. The current paper presents data relating to microbial apatitic systems that strengthen this hypothesis.  相似文献   

POST-PARTURIENT HAEMOGLOBINURIA OF DAIRY COWS     

B. Parkinson  B.V.Sc. A. K. Sutherland  B.V.SC  M.S. 《Australian veterinary journal》1954,30(8):232-236

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SEROLOGICAL DIAGNOSIS OF LARVAL CESTODES IN SHEEP     

Sally K.  Blundell-Hasell M.SC. 《Australian veterinary journal》1969,45(7):334-336

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THE BOVINE MUCOSAL DISEASE-SWINE FEVER VIRUS COMPLEX IN PIGS   总被引：2，自引：0，他引：2  

W. A. Snowdon  B.V.SC. E. L. French  M.SC  Ph.D. 《Australian veterinary journal》1968,44(4):179-184

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STUDIES ON THE TRANSMISSIBILITY OF A VIRAL MENINGO-ENCEPHALITIS OF CALVES     

L. A. Y. Johnston  B.V.SC.  G. C. Simmons  B.SC. M. D. McGavin  M.V.SC. 《Australian veterinary journal》1964,40(5):189-194

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HYDATID DISEASE IN AUSTRALIA     

Michael A.  Gemmell B.V.SC. P. Brydon  B.V.SC. 《Australian veterinary journal》1960,36(3):73-78

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