Bovine Leukocyte Adhesion Deficiency in Danish Holstein-Friesian Cattle: I. PCR Screening and Allele Frequency Estimation     

C.B. Jrgensen  J.S. Agerholm  J. Pedersen  P.D. Thomsen 《Acta veterinaria Scandinavica》1993,34(3):231

Jergensen, C. B., J. S. Agerholm, J. Pedersen and P. D. Thomsen: Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian Cattle I. PCR screening and allele frequency estimation. Acta vet. Scand. 1993,34,231-236.–A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder.  相似文献   

A survey of northern Victorian dairy farmers to investigate dairy calf management: colostrum feeding and management          下载免费PDF全文

AJ Phipps  DS Beggs  AJ Murray  PD Mansell  MF Pyman 《Australian veterinary journal》2018,96(4):101-106

Objectives

To describe colostrum management practices carried out in northern Victorian dairy herds and to identify weaknesses in these areas that may affect calf health and welfare by comparing the results with the current industry recommendations

Methods

A questionnaire to obtain information about colostrum management and calf‐rearing practices was sent to commercial dairy farming clients of Rochester Veterinary Practice between June and September 2013. The questionnaire consisted of a general herd overview and colostrum harvesting practices.

Results

The response rate was 39% (58/150). Many dairy producers were not meeting the current industry recommendations in the following areas: (1) time of removal calf from the dam, (2) relying on calf suckling colostrum from the dam to achieve adequate passive transfer, (3) failing to supplement calves with colostrum, (4) feeding inadequate volumes of colostrum, (5) delayed colostrum harvesting, (6) pooling of colostrum, (7) failing to objectively assess colostrum quality or relying on visual assessment and (8) storing colostrum for a prolonged periods of time at ambient temperatures.

Conclusion

The results from this survey highlight the need for greater awareness of industry standards for colostrum management and feeding hygiene.

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Loser cows in Danish dairy herds: definition, prevalence and consequences     

Thomsen PT  Østergaard S  Sørensen JT  Houe H 《Preventive veterinary medicine》2007,79(2-4):116-135

During the last few years, many Danish dairy farmers have expressed increasing concerns regarding a group of cows, which we have chosen to term ‘loser cows’. Until now, a loser cow has not been described scientifically. We defined a loser cow on the basis of a clinical examination of the cow. A total of 15,151 clinical examinations were made on 6,451 individual cows from 39 randomly selected, large Danish dairy herds with loose-housing systems using a clinical protocol. Scores for the clinical signs lameness, body condition, hock lesions, other cutaneous lesions, vaginal discharge, condition of hair coat and general condition were converted into a loser cow score. Cows with a loser cow score of 8 or more were classified as loser cows. The overall prevalence of loser cows was 2.15%, 4.50% and 2.98% during the first, second and third round of herd visits, respectively.

The associations between the loser cow state and milk production, mortality, morbidity, culling and workload for the farmer were evaluated using data from herd visits and from the Danish Cattle Database and a number of different statistical techniques. It was concluded that the loser cow state has significant negative consequences for both the farmer and the cow. On average, loser cows yielded 0.61 to 2.24 kg energy corrected milk less per day than non-loser cows depending on parity. Hazard ratio for death or euthanasia was 5.69 for loser cows compared to non-loser cows. Incidence rate ratio for disease treatments was 0.69 for non-loser cows compared to loser cows. Loser cows were often culled in an ‘unfavourable’ way and generally caused extra workload for the farmer.

A simplified version of the loser cow score was evaluated and is recommended for future research and use in practice.  相似文献   

Sex Determination of Bovine Embryo Biopsies Using in situ Hybridization*     

P.D. Thomsen  M. Schwerin  P.H. Poulsen  B. Avery 《Reproduction in domestic animals》1991,26(2):66-69

A male specific bovine DNA fragment was used as probe to determine the sex of bovine interphase cells by in situ hybridization. This method also proved useful for determining the sex of bovine embryos.  相似文献   

Mapping of quantitative trait loci for lactation persistency traits in German Holstein dairy cattle     

B. Harder  J. Bennewitz  N. Reinsch  G. Thaller  H. Thomsen  C. Kühn  M. Schwerin  G. Erhardt  M. Förster  F. Reinhardt  & E. Kalm 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(2):89-96

A whole genome scan to map quantitative trait loci (QTL) for persistency of milk yield (PMY), persistency of fat yield (PFY), persistency of protein yield (PPY) and persistency of milk energy yield (PEY) was performed in a granddaughter design in the German Holstein dairy cattle population. The analysis included 16 paternal half‐sib families with a total of 872 bulls. The analysis was carried out for the first lactation and for the first three lactations combined using univariate weighted multimarker regression. Controlling the false discovery rate across traits and data sets at a level of 0.15 and treating the four persistency traits as different traits revealed 27 significant QTL. A total of 12 chromosomes showed significant QTL effects on a chromosomewise basis. The DGAT1 effect was highly significant for PPY and protein yield. A haplotype analysis using results of previous studies of the same design revealed a co‐segregation of various persistency QTL and QTL affecting health traits like dystocia and stillbirth and functional traits like non‐return rate 90 and somatic cell score.  相似文献   

Effects of proinflammatory mediators on canine neutrophil chemotaxis and aggregation     

H Str?m  M K Thomsen 《Veterinary immunology and immunopathology》1990,25(3):209-217

The present study investigated in vitro the qualitative and quantitative neutrophil function-activating properties of the most important non-cytokine participants in the acute inflammatory cell response. Apart from the results obtained with leukotriene (LT) D4, similar qualitative relationships were found for the several mediators tested regarding effects on neutrophil migration and aggregation. Thus, LTB4, PAF-acether and zymosan-activated serum all activated both functions, while f-Met-Leu-Phe had no effect. In all cases, the half-maximal eliciting concentration (EC50) for induction of chemotaxis was much lower than for aggregation, indicating that high and low receptor affinity responses were being studied, respectively. LTD4 induced modest aggregation, but was virtually without effect on migration. Using PAF-acether as stimulus, the mechanism of aggregation was studied in more detail. The results indicate that LTB4, PAF-acether, the complement-split products C5a/C5a desArg, and perhaps LTD4, may play a role as stimulants of neutrophil functions in inflammatory processes in vivo.  相似文献   

Morphometry,Morphology and Ultrastructure of Ring‐tailed Coati Sperm (Nasua nasua Linnaeus, 1766)          下载免费PDF全文

HVR Silva  FF Magalhães  LR Ribeiro  ALP Souza  CIA Freitas  MF de Oliveira  AR Silva  LDM Silva 《Reproduction in domestic animals》2015,50(6):945-951

The ring‐tailed coati (Nasua nasua) is a procyonid whose population is in sharp decline. Therefore, studies are needed to better understand the reproduction of this animal. For this reason, this study aimed to evaluate the morphology, morphometry and sperm ultrastructure of ring‐tailed coati sperm. Four captive adult males were used for this study. Slides stained with Bengal Rose were used for the morphometric and morphologic analyses. The length and width of the head were measured, as well as the length of the midpiece and tail and the total length of the sperm. Scanning electron microscopy and transmission electron microscopy were used for the ultrastructural analyses. The most obvious morphological abnormalities observed were coiled tails (6.1 ± 8.7%) and the lack of acrosomes (5.4 ± 4.4%). Regarding the morphometry, the measurements of the head (length × width), midpiece (length) and tail (length) were (mean ± SD) 6.2 ± 0.4 × 8.1 ± 0.6 μm, 14.1 ± 0.5 and 63.9 ± 4.1 μm, respectively, and the total length of the sperm was 86.1 ± 4.3 μm. Through electron microscopy, the presence of electron‐lucent points in the nucleus and the presence of approximately 55 mitochondrial spirals in the midpiece were identified. The data obtained in this study provide detailed information on the sperm characteristics of coatis and may inform future research on germplasm conservation, both for this species and other threatened procyonids.  相似文献   

The effect of footrot on body weight and wool growth of sheep   总被引：1，自引：0，他引：1  

DJ MARSHALL  RI WALKER  BR CULLIS  MF LUFF 《Australian veterinary journal》1991,68(2):45-49

Body weight and traits associated with production of wool were measured over a 2-year period between 1985 and 1987 in south-western New South Wales in a flock of Merino wethers experimentally infected with footrot. The disease was allowed to spread freely amongst 150 of the flock but kept at very low prevalence in the remaining 50 by preventive footbathing during transmission periods. Severe, underrunning footrot had a significant adverse effect on body weight, for each year of the trial. Body weight was most severely reduced at times of the year when footrot was spreading among animals and lesions were severe. The mean body weight of the infected group at the end of the 2 years of observation was 7.3 kg (11.6%) below that of the control group. Footrot also depressed wool growth, with the mean clean fleece weight of the infected group being 0.4 kg (8%) lighter than that of the controls at each of the 2 annual shearings. There were no consistent differences between the groups for the other wool characteristics measured.  相似文献   

Probing the Local Effects of Magnetic Impurities on Superconductivity     

A Yazdani  BA Jones  CP Lutz  MF Crommie  DM Eigler 《Science (New York, N.Y.)》1997,275(5307):1767-1770

The local effects of isolated magnetic adatoms on the electronic properties of the surface of a superconductor were studied with a low-temperature scanning tunneling microscope. Tunneling spectra obtained near magnetic adsorbates reveal the presence of excitations within the superconductor's energy gap that can be detected over a few atomic diameters around the impurity at the surface. These excitations are locally asymmetric with respect to tunneling of electrons and holes. A model calculation based on the Bogoliubov-de Gennes equations can be used to understand the details of the local tunneling spectra.  相似文献   

Placers of cosmic dust in the blue ice lakes of greenland     

Maurette M  Hammer C  Reeh N  Brownlee DE  Thomsen HH 《Science (New York, N.Y.)》1986,233(4766):869-872

A concentration process occurring in the melt zone of the Greenland ice cap has produced the richest known deposit of cosmic dust on the surface of the earth. Extraterrestrial particles collected from this region are well preserved and are collectable in large quantities. The collected particles are generally identical to cosmic spheres found on the ocean floor, but a pure glass type was discovered that has not been seen in deep-sea samples. Iron-rich spheres are conspicuously rare in the collected material.  相似文献