排序方式： 共有17条查询结果，搜索用时 203 毫秒
1.
Ravindra DONDE Jitendra KUMAR Gayatri GOUDA Manoj Kumar GUPTA Mitadru MUKHERJEE Sk Yasin BAKSH Pradosh MAHADANI Khirod Kumar SAHOO Lambodar BEHERA Sushanta Kumar DASH 《水稻科学》2019,26(4):239-247
The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines(CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472,RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi,RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content(PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought. 相似文献
2.
Michael Klotsman Gayatri Sathyan Wayne H. Anderson Oliver A. Garden Padmaja Shivanand 《Journal of veterinary pharmacology and therapeutics》2019,42(2):127-138
Mycophenolic acid (MPA), a noncompetitive, selective and reversible inhibitor of inosine 5′‐monophosphate dehydrogenase (IMPDH), is an immunosuppressive agent that has a long history in medicine. Mechanistically, the inhibition of IMPDH leads to the selective and eventual arrest of T‐ and B‐lymphocyte proliferation. Mycophenolate mofetil (MMF), the first MPA‐based product to receive marketing approval over two decades ago, was originally indicated for the prophylaxis of organ rejection in human transplant patients. Given its broad immunosuppressive properties and ability to selectively inhibit lymphocyte division and effector functions, the clinical utility of MPA was subsequently explored in a host of autoimmune diseases. Human clinical studies have shown MPA to be safe and effective and support its off‐label administration for immune‐mediated diseases such as lupus, myasthenia gravis and atopic dermatitis. MMF became generically available in the United States in 2008, and its clinical utility is increasingly being explored as a treatment option for dogs with immune‐mediated diseases. This review summarizes the available literature for MPA pharmacokinetics and pharmacodynamics, and the current status of MPA as a treatment for client‐owned dogs diagnosed with immune‐mediated diseases. 相似文献
3.
Megha Kadam Bedekar Praveena Soman Sajal Kole Deepika Anand Gayatri Tripathi M. Makesh K. V. Rajendran 《Aquaculture International》2018,26(6):1401-1413
Interferon gamma (IFN-γ) or type II interferon is a cytokine that is critical for innate and adaptive immunity against viral and some bacterial and protozoal infections. The importance of IFN-γ in the immune system lies in its ability to inhibit viral replication directly and most importantly from its immunomodulatory effects. Previously, we successfully co-administered IFN-γ along with GAPDH gene of Edwardsiella tarda as bicistronic DNA vaccine in Labeo rohita. In order to ascertain the individual role of IFN-γ, the present study involves cloning and expression of 552-bp IFN-γ open-reading frame (ORF) of L. rohita in striped snakehead (SSN-1) cell line using eukaryotic expression vector system (pQE-TriSystem) followed by transfection in peripheral blood lymphocytes (PBMCs) to evaluate its immunomodulatory ability in comparison to polyinosinic-polycytidylic acid (Poly I:C)-treated PBMCs. The 18.7-kDa protein, expressed in the pQE-IFNγ-transfected SSN-1 cells, reacted with anti-His antibody in Western blot confirming it to be recombinant IFN-γ, whereas the relative expression of IFN-γ, iNOS, Mx, and IL-1β genes in PBMCs was quantified at 24 h and 48 h post treatment by qPCR. The comparative kinetics of all four genes showed significantly (p?<?0.05) high upregulation pattern in both pQE-IFNγ-transfected cell group and Poly I:C-treated cell group demonstrating recombinant IFN-γ as an equally efficient inducer like Poly I:C. Thus, our in vitro experiment results highlight the immunomodulatory potential of recombinant IFN-γ as an analogue to synthetic Poly I:C which warranted future studies to further explore the potential of recombinant IFN-γ as an effective vaccine adjuvant against different microbial invasion. 相似文献
4.
Dinesh Kumar Benbi Amardeep Singh Toor Gayatri Verma 《Communications in Soil Science and Plant Analysis》2016,47(15):1828-1844
The study was conducted to investigate the aggregate stability and distribution of organic carbon (C) in different-sized aggregates and mineral fractions in a loamy soil under rice-wheat system with continuous application of fertilizer nitrogen, phosphorus and potassium (NPK), farmyard manure (FYM), N+FYM and NPK+FYM, compared with unfertilized control. Macro-aggregates comprised 37.1–49.3% of the total water-stable aggregates (WSAs), compared with 23.3–30.3% as micro-aggregates. Application of inorganic fertilizers and FYM significantly increased the proportion of macro-aggregates, which were linearly related to total organic C (TOC). Organic C concentration in coarse macro-aggregates (CMacA) was higher than the micro-aggregates (CMicA). Application of FYM significantly increased the concentration of organic C in different-sized aggregates and mineral fraction, compared with the unfertilized control. Averaged across treatments, mineral-associated C comprised 26% of TOC. Macro-aggregates, on an average, constituted 66–68% of C preservation capacity of WSA. The amount of TOC sequestered was higher when NPK+FYM was applied together. 相似文献
5.
Sushila Ngairangbam Das Basanta Kumar Prasad K. Pani Tripathi Gayatri 《Aquaculture International》2021,29(6):2387-2402
Aquaculture International - Quantitative real-time RT-PCR (qRT-PCR) is the most sensitive and advanced method to quantify the expression of target genes of animals including fish. However, the... 相似文献
6.
Rathinam R. Bharathi Iburahim S. Abuthagir Ramanan S. Suresh Tripathi Gayatri 《Aquaculture International》2022,30(1):341-363
Aeromonas spp are the most common devastating fish bacterial pathogens associated with a wide range of cultured freshwater fish resulting in mass mortality. The present study plots the Aeromonas research output in fish during 1998–2020 by means of the Web of Science (WoS) database. A total of 1944 publications were used for this investigation to evaluate the scientific productivity in this selected subject area. Global publication share, rank, and its development in terms of research and bibliometric indices such as overall citations, h-index, and average citation per paper were also analysed. China ranked first in terms of total publications among the most productive countries and accounted for 26.18% of the total publications. The network visualisation map revealed that the lead collaborating country was China; however, the collaboration between countries was found to be very less. Lokta’s law was noticed not to be valid for Aeromonas research. The study showed that the funding granted to the research investigations and their research productivity in terms of total publications were correlated positively. The results of the current study displayed the trend of “Aeromonas infection in fish” related research investigations published during 1998–2020. This study also highlighted the focused research areas on this topic on which the future areas of research can be determined by fellow researchers in this specific field. This study also suggests enhancing the research efforts on this bacterial pathogen which has the potential to implicate huge economic loss to the fish farming population in the world.
相似文献7.
基于JKR粘结模型的蚯蚓粪基质离散元法参数标定 总被引：8，自引：0，他引：8
为确定不同含水率下蚯蚓粪基质的多种参数,提出了通过测定基质含水率,预测休止角,通过休止角合理推测其他参数的思路,并提出了一种散体休止角测定方法。以休止角作为参照,基于JKR粘结模型,使用离散元参数标定的方法,从与蚯蚓粪基质颗粒有关的10个参数中,筛选出颗粒间静摩擦因数、颗粒间滚动摩擦因数和JKR表面能3个对休止角影响显著的参数,建立了休止角与这3个显著参数之间的二次多项式回归模型。试验结果表明,该模型可以根据休止角预测蚯蚓粪基质参数,根据预测得到的参数建立离散元模型,休止角仿真结果与实际试验结果较为接近,差异分别为1.53%和0.22%。同时测定了不同含水率下蚯蚓粪基质的休止角,建立了休止角与含水率之间的关系模型。研究结果可为其他类似散体物料休止角的测定提供参考,并提供了一种通过测定易于测定的参数(如含水率)来推导其他难测参数的思路。 相似文献
8.
In vitro storage of fish oocytes: effect of storage temperature,media conditions and storage duration on fertilization and larval hatchability of Indian major carp,rohu (Labeo rohita) 
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下载免费PDF全文 Gayatri Mishra Sunita Patra Santosh Kumar Dash Dhananjay Kumar Verma Padmanav Routray 《Aquaculture Research》2017,48(5):2486-2494
The viability of matured oocytes stored in vitro were assessed using carp ovarian fluid (OF) and artificial carp ovarian fluid (ACOF) under different temperature regimes (4, 24, 26, 28 and 30°C) for different storage durations (0, 60, 120, 150 and 180 min). Significantly higher fertilization (74%) was achieved when the oocytes were stored using ACOF and 65% in OF after 180 min at 28°C. Similarly the hatching rates of larvae were significantly higher in the ACOF and OF, that is, 64% and 47%, respectively, after 180 min of storage. The oocytes kept in the storage containers with ACOF having 65% moisture level showed a significantly higher fertilization rate than the 59% moisture level. This study demonstrated that unfertilized matured oocytes (eggs) of rohu can be stored in vitro for 180 min without compromising the viability (fertilization and hatching) to a great extent in OF and ACOF. 相似文献
9.
Tianxiao Chen Sergey Shabala Yanan Niu Zhong-Hua Chen Lana Shabala Holger Meinke Gayatri Venkataraman Ashwani Pareek Jianlong Xu Meixue Zhou 《作物学报(英文版)》2021,(3):506-520
Salinity is one of the major abiotic stresses which impose constraints to plant growth and production. Rice (Oryza sativa L.) is one of the most important stapl... 相似文献
10.
Shalini Pulipati Suji Somasundaram Nitika Rana Kavitha Kumaresan Mohamed Shafi Peter Civ &#x Gothandapani Sellamuthu Deepa Jaganathan Prasanna Venkatesan Ramaravi S. Punitha Kalaimani Raju Shrikant S. Mantri R. Sowdhamini Ajay Parida Gayatri Venkataraman 《水稻科学》2022,29(1):31-46
Asian cultivated rice shows allelic variation in sodium transporter, OsHKT1;5, correlating with shoot sodium exclusion (salinity tolerance). These changes map to intra/extracellularly-oriented loops that occur between four transmembrane-P loop-transmembrane (MPM) motifs in OsHKT1;5. HKT1;5 sequences from more recently evolved Oryza species (O. sativa/O. officinalis complex species) contain two expansions that involve two intracellularly oriented loops/helical regions between MPM domains, potentially governing transport characteristics, while more ancestral HKT1;5 sequences have shorter intracellular loops. We compared homology models for homoeologous OcHKT1;5-K and OcHKT1;5-L from halophytic O. coarctata to identify complementary amino acid residues in OcHKT1;5-L that potentially enhance affinity for Na+. Using haplotyping, we showed that Asian cultivated rice accessions only have a fraction of HKT1;5 diversity available in progenitor wild rice species (O. nivara and O. rufipogon). Progenitor HKT1;5 haplotypes can thus be used as novel potential donors for enhancing cultivated rice salinity tolerance. Within Asian rice accessions, 10 non-synonymous HKT1;5 haplotypic groups occur. More HKT1;5 haplotypic diversities occur in cultivated indica gene pool compared to japonica. Predominant Haplotypes 2 and 10 occur in mutually exclusive japonica and indica groups, corresponding to haplotypes in O. sativa salt-sensitive and salt-tolerant landraces, respectively. This distinct haplotype partitioning may have originated in separate ancestral gene pools of indica and japonica, or from different haplotypes selected during domestication. Predominance of specific HKT1;5 haplotypes within the 3 000 rice dataset may relate to eco-physiological fitness in specific geo-climatic and/or edaphic contexts. 相似文献