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1.
Identification of new parental lines is crucial for developing ecology‐specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology‐specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3‐10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga‐based CMS lines. All the 80 F1s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding.  相似文献   
2.
Eremostachys superba Royle ex Benth. (Lamiaceae) has undergone a severe decline in population size since its discovery in the North-western Himalayas in late 19th century. One hundred and seventy-two plants from six populations in the Indian states of Uttar Pradesh and Jammu & Kashmir, located between 0.45 km and 455.72 km apart from each other were evaluated for RAPD polymorphism. Sixteen random primers generated 92 bands overall, 77 of which were polymorphic. Shannon’s index of genetic diversity within populations (H o) ranged between 0.305 and 0.421; the average within-population diversity (H pop) was 0.389; and the total species diversity (H sp) was 0.478. The population from Mohand (representing the type locality) had the fewest plants, at 18, and was genetically the most depauperate. Among the other populations, ranging in size between 52 and 1,022 individuals, no relation between population size and genetic diversity was evident. It is suggested that these six populations represent relics of a larger, extended population, in which the presence of perennating rootstocks has helped preserve historic patterns of genetic diversity. AMOVA revealed that 83.01% of the variation exists within populations, which was consistent with earlier studies on the reproductive biology of E. superba, which indicated this species is predominantly allogamous. FST distances between all populations were significant, indicating geographic differentiation despite some of them being closely separated. Habitat restoration and protection from indiscriminate harvesting are proposed as primary strategies for conserving E. superba. Rejuvenation of the Mohand population through intrapopulation crossing between plants bearing diverse molecular phenotypes is also suggested.  相似文献   
3.
In the Indo-Gangetic plain (India), a sequence of soils, developed under different climatic conditions has been investigated micromorphologically to study the evolution of the pedogenic calcite formations. The climate of the plain grades from arid and hot to semi-arid and hot to less hot, and the soils, respectively, from Camborthids, through Calciorthids and Ustochrepts, to Haplustalfs.  相似文献   
4.
Energy flow in biological structures often requires submillisecond charge transport over long molecular distances. Kinetics modeling suggests that charge-transfer rates can be greatly enhanced by multistep electron tunneling in which redox-active amino acid side chains act as intermediate donors or acceptors. We report transient optical and infrared spectroscopic experiments that quantify the extent to which an intervening tryptophan residue can facilitate electron transfer between distant metal redox centers in a mutant Pseudomonas aeruginosa azurin. Cu(I) oxidation by a photoexcited Re(I)-diimine at position 124 on a histidine(124)-glycine(123)-tryptophan(122)-methionine(121) beta strand occurs in a few nanoseconds, fully two orders of magnitude faster than documented for single-step electron tunneling at a 19 angstrom donor-acceptor distance.  相似文献   
5.
6.
Aquaculture International - The current study evaluated the biosafety of oxytetracycline (OTC) exposure for 30 days in monosex Oreochromis niloticus fries. The fries were exposed to OTC...  相似文献   
7.
Gonad abnormalities can restrict or completely block reproductive capability of individuals and in some case that of their populations. Here, we describe a novel testicular degenerative condition of non-germ cell origin with a high prevalence (up to 22.1% of the population) in a wild population of carp. Based on gross morphology, and microscopic and cellular examinations, the condition shows progressive severity which could be categorized into low, mild, severe and complete. In early stages of the condition, an abnormally increased proliferation (11-fold) of the Sertoli cell occurred, followed by degenerative cell death of all testicular cells, resulting in fluid-filled vesicles in the later stages. This initial uncontrolled proliferation of Sertoli cells suggests that the condition could be triggered by malignant pathways; however, the observed subsequent apoptosis of all testicular cells en masse, rendering the animals “sterile,” appears unique. Observations, to date, indicate that this condition is specific to male carp and not present in other species of fish sharing the habitat. High prevalence of the condition allowed comparative evaluation between affected individuals, an aspect likely to facilitate future studies, including elucidation of the cause, robust testing of therapies and practical applications such as management of feral carp populations.  相似文献   
8.
With an overarching objective of improving the hatchery production of Atlantic salmon (Salmo salar) all‐female progeny, this study comparatively evaluated the reproductive parameters between normal (genotype XY) and neo‐males (genotype XX). Four normal (XY) and seven neo‐ (XX) males, from the same brood stock, distinguished by their ability to or lack of expressing semen, respectively, were comparatively evaluated. The left testicular lobe was used for histomorphometric analyses, while the right for semen collection and sperm quality analyses. Histomorphometric observations revealed that neo‐male testes are irregularly shaped, and have poorly formed seminiferous ducts, higher proportions of interstitial tissue and lower gonadosomatic index (p < .05). In addition, hypertrophied and cyst forming Sertoli cells were found in these individuals which collectively appear to form a physical barrier, precluding the semen collection by standard stripping techniques and reducing sperm quality. Particularly, semen motility (80.69 ± 2.4% and 57.2 ± 36.5% for XY and XX respectively) and duration of motility (99.31 ± 28.03 s and 66.84 ± 23.83 s for XY and XX respectively) of neo‐males were most compromised (p < .05). Interestingly, the TUNEL assay indicated no signs of apoptotic tissue suggesting that the histological differences may relate to delayed physiological/sexual maturity of neo‐males.  相似文献   
9.
Most of the salt-affected soils of the Indo-Gangetic plain in NW India do not find suitable places in the 1970 U.S. system of soil classification. According to the current class definitions, soils with high salts, ESP, pH, chromas and yellower hues key out as Typic or Aquic Calciorthids, Camborthids and Haplustalfs which does not spell out their saline-sodic nature. For land-use recommendations, the authors believe it will be useful to set these soils apart at some high categoric level in the system.It is proposed that the structural requirements for the natric horizon be modified to include horizons with high ESP (? 40) but having simple blocky structure with or without tongues of eluvial material. New subgroups, viz. Natric, within the orders of Inceptisols, Alfisols and Aridisols is suggested for the high sodium-saturated soils lacking natric horizons. For similar practical considerations, the high concentrations of salts in soils when associated with high ESP pose problems in leaching and consequently new subgroups, viz. Salic and Salic Natric, within the orders of Aridisols and Alfisols are suggested.The modifications proposed not only place more emphasis on the saline-sodic nature of these soils but also result in better groupings for practical land use.  相似文献   
10.
A reconnaissance soil survey of the NW Himalayan mountains and highlands in the Lahul valley (Himachal Pradesh) was undertaken and five soil profiles from different regions and supporting different vegetation, representing both virgin and cultivated lands, were studied, described and classified in two major soil series, viz. Krozing and Shainsha. The clay fraction of these soils consists of illite and chlorite with minor amounts of kaolinite and traces of swelling chlorite and/or montmorillonite. The removal of amorphous material by a dithionite citrate buffer has been found to be imperative.According to the current definitions, these frigid like high mountain soils having an epipedon on limit with mollic qualify for Dystric Eutrochrepts, which does not depict the reality. Therefore, they have been logically classified, by defining new subgroups of Cryic, Mollic and Cryollic within Eutrochrepts, as Dystric Cryic Eutrochrepts (Shainsha series) and Dystric Cryollic Eutrochrepts (Krozing series). The proposed classification is supported by their geographic situation between Eutrochrepts and/or Hapludolls (in lower valleys) and Cryochrepts and/or Haploborolls (on higher hills).  相似文献   
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