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1.
Seyedeh Masoumeh Fotoukkiaii Zoë Tan Wenxin Xue Nicky Wybouw Thomas Van Leeuwen 《Pest management science》2020,76(3):1154-1163
2.
Arya Vazirzadeh Abdolmajid Hajimoradloo Hamid Reza Esmaeili Mostafa Akhlaghi 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):267-269
The effects of saline-dissolved or Freund's incomplete adjuvant (FIA)-emulsified GnRHa treatment on the induction of ovulation in rainbow trout, Oncorhynchus mykiss, were examined. The FIA-emulsified GnRHa was first diluted in 0.25 ml physiological saline and then mixed with an equal volume of FIA. Fish were selected in the beginning of the spawning season and were allocated into four groups and were treated intraperitoneally with (a) 0.5 ml of emulsified GnRHa (GnRHa–FIA), (b) 0.5 ml saline-dissolved GnRHa in a single injection (GnRHa-1), (c) 0.5 ml saline-dissolved GnRHa in two injections spaced 1 d apart (GnRHa-2) and (d) 0.5 ml of saline (Control). The GnRHa dose in all hormone treatments was 25 µg kg− 1. All fish in the FIA–GnRHa and GnRHa-2 groups ovulated within 10 and 11 d after treatment, respectively. In contrast, only 75% in the Control fish and 60% of the fish in the GnRHa-1 group ovulated within 36 d after treatment. None of the treatments caused any pre- or post-spawning mortality in the broodstock. Fertilization, eyeing and hatching percentages of the produced progeny were normal in all the treatment groups and did not differ significantly among them. In conclusion, FIA-emulsified GnRHa can be effective in advancing the onset of and synchronizing the ovulation of rainbow trout within a two-week period, thus shortening the egg collection period, without affecting broodstock survival and egg quality. 相似文献
3.
Passive immunisation of fish against vibriosis, comparison of intraperitoneal, oral and immersion routes 总被引：2，自引：0，他引：2
M. Akhlaghi 《Aquaculture (Amsterdam, Netherlands)》1999,180(3-4):191-205
Passive immunisation of fish was conducted to determine whether anti-Vibrio anguillarum whole sera (AVA) and affinity-purified AVA raised in sheep, rabbits and rainbow trout (Oncorhynchus mykiss) were persistent when injected and orally administered into rainbow trout. These responses were compared with active immunisation by immersion in, and intraperitoneal (i.p.) injection with, formalin-killed V. anguillarum cells. Sheep and rabbit AVA were detected in rainbow trout sera for up to 70 days (half-life 21 days) after i.p. injection as determined by an enzyme-linked immunosorbent assay (ELISA). The relative percentage survival (RPS) of passively immunised rainbow trout challenged with virulent V. anguillarum after an injection was comparable to that of active immunisation by immersion after 1 month post-immunisation (p.i). Affinity-purified sheep and rabbit AVA exhibited the same protective potential as whole serum in rainbow trout. Rabbit and sheep immune sera diluted 1:8 and 1:50, respectively, provided equivalent protection as undiluted fish immune serum. An active immune response against passively acquired heterologous immunoglobulins was demonstrated by ELISA, with responses against sheep AVA being less than those against rabbit AVA. Rainbow trout given purified sheep AVA conjugated to LTB (the GM-1-binding subunit of Escherichia coli heat-labile toxin) and administered orally had an RPS of 37.5% at 15 days and 27% at 1 month p.i. In contrast, fish given sheep AVA conjugated to TraT (an internal membrane of E. coli) or in micellar form with Quil-A had RPSs of only 18.7 and 6.2%, respectively, after 15 days, and 13.3 and 0% after 1 month, respectively. The protection conferred by immune sera was shown to be due to the immunoglobulin component alone. Heat inactivation of the complement in sera had no effect on the potency of immune sera. 相似文献
4.
Masoumeh Afzali Alireza Nakhaee Seyed Payman Tabatabaei Kourosh Tirgar-Fakheri Mohammad Hashemi 《Iranian Biomedical Journal》2013,17(2):77-83
Background: The protein of Niemann-pick type C1 (NPC1) gene promotes the egress of cholesterol from late endosomes and lysosomes to other cellular compartments and contributes to a process known as reverse cholesterol transport. This study aimed to examine whether promoter methylation of NPC1 is associated with risk of cardiovascular disease (CVD). Methods: Fifty CVD patients and 50 healthy subjects as the control group were recruited in this study. Promoter methylation of NPC1 gene was defined using a nested-methylation specific polymerase chain reaction method. Statistical analyses were done using the chi-square, t-test or ANOVA tests. Results: Our study showed that the frequency of semi-methylated promoter (methylated/unmethylated status) was significantly higher in CVD patients than that in controls (OR = 6.521, 95% CI = 2.211-19.215, P = 0.008). However, a completely methylated promoter (methylated/methylated status) was not detected in any subjects in either of the two groups tested. Additionally, the analysis of clinical data according to the methylation status of NPC1 gene demonstrated that serum levels of total cholesterol, total triglycerides, high low-density lipoprotein cholesterol (LDL-C) and low high-density lipoprotein cholesterol (HDL-C) are influenced by NPC1 methylation, so that subjects with a completely unmethylated promoter (Unmethylated/unmethylated status) held lower levels of total triglycerides, total cholesterol, LDL-C and higher levels of HDL-C. Conclusion: Our findings propose that the NPC1 promoter methylation is a probable mechanism that can result in reduced/impaired NPC1 expression/activity and may thus contribute to progression of CVD. Key Words: Niemann-pick type C1 (NPC1), Promoter methylation, cardiovascular disease 相似文献
5.
Hadi Atashi Jamshid Izadifard Mohammad Javad Zamiri Amir Akhlaghi 《Tropical animal health and production》2013,45(4):1051-1054
In the present study, 1,635 lambing records of Ghezel (n?=?766) and Mehraban (n?=?869) breeds were used to evaluate the early growth traits, litter size, and lamb survival in sheep reared in Fars Province, southern Iran, during a 5-year-long period. The least squares means (± SE) of lamb birth weight for Ghezel were 5.27 (±0.22), 5.02 (±0.22), and 3.98 (±0.23) kg for single males, single females, and twin lambs, respectively; whereas, the corresponding values for Mehraban were 4.39 (±0.18), 4.18 (±0.18), and 3.50 (±0.19) kg. The least squares means of pre-weaning lamb growth (gram per day) for Ghezel were 239 (±15), 218 (±15), and 181 (±16) for single males, single females, and twin lambs, respectively, and the corresponding values for Mehraban were 204 (±12), 187 (±12), and 156 (±13). Lambs from 2 year old ewes or younger were on average lighter at birth and at weaning and had a lower average daily gain than those from older ewes (P?<?0.05). The percentage of twin births increased from 1.1 and 1.3 % for ewes aged ≤ 2 years to 19 and 12 % for those aged ≥ 6 years old in Ghezel and Mehraban, respectively (P?<?0.05). The mean pre-weaning lamb mortality was 7.1 and 4.1 % for Ghezel and Mehraban, respectively. 相似文献
6.
7.
Hossein Ali Ghaffaripour Mehdi Jalali Mohammad Reza Nikravesh Masoumeh Seghatoleslam Javad Sanchooli 《Iranian Biomedical Journal》2015,19(1):29-34
Background: Brain hypoxia-ischemia is a human neonatal injury that is considered a candidate for stem cell therapy. Methods: The possible therapeutic potential of human umbilical cord blood (HUCB) stem cells was evaluated in 14-day-old rats subjected to the right common carotid occlusion, a model of neonatal brain hypoxia-ischemia. Seven days after hypoxia-ischemia, rats received either saline solution or 4 × 105 HUCB cells i.v. Rats in control group did not receive any injection. After two weeks, rats were assessed using two motor tests. Subsequently, rats were scarified for histological and immunohistochemical analyses. Results: Our immunohistochemical findings demonstrated selective migration of the injected HUCB cells to the ischemic area as well as reduction in infarct volume. Seven days after surgery, we found significant recovery in the behavioral performance in the test group (12.7 +/- 0.3) compared to the sham group (10.0 +/-0.05), a trend which continued to day 14 (15.3 ± 0.3 vs. 11.9 ± 0.5, P<0.05). Postural and motor asymmetries at days 7 and 14 in the test group showed a significant decrease in the percentage of right turns in comparison to the sham group (75% and 59% vs. 97% and 96%, P<0.05). Conclusion: The results show the potential of HUCB stem cells in reduction of neurologic deficits associated with neonatal hypoxia-ischemia. Key Words: Hypoxia-Ischemia, Nerve cell, Umbilical Cord Blood 相似文献
8.
Farahnaz-Sadat Shayganmehr Masoud Alebouyeh Masoumeh Azimirad Mohammad Mehdi Aslani Mohammad Reza Zali 《Iranian Biomedical Journal》2015,19(3):143-148
Background:
Reduced susceptibility of Clostridium difficile to antibiotics is problematic in clinical settings. There is new evidence indicating the cotransfer of toxin-encoding genes and conjugative transposons encoding resistance to antibiotics among different C. difficile strains. To analyze this association, in the current study, we evaluated the frequency of toxigenic C. difficile among the strains with different multidrug-resistant (MDR) profiles in Iran.Methods:
Antimicrobial susceptibility patterns and minimal inhibitory concentrations (MIC) of the isolates were determined against metronidazole, imipenem, ceftazidime, amikacin, and ciprofloxacin by agar dilution method. The association of the resistance profiles and toxigenicity of the strains were studied by PCR targeting tcdA and tcdB genes.Results:
Among 86 characterized strains, the highest and lowest resistance rates were related to ciprofloxacin (97%) and metronidazole (5%), respectively. The frequency of resistance to other antibiotics was as follow: imipenem (48%), ceftazidime (76%), and amikacin (76.5%). Among the resistant strains, double drug resistance and MDR phenotypes were detected in the frequencies of 10.4% and 66.2%, respectively. All of the metronidazole-resistant strains belonged to tcdA +/tcdB + genotype with triple or quintuple drug resistance phenotypes. MIC50 and MIC90 for this antibiotic was equally ≤ 8 μg/ml.Conclusion:
These results proposed the association of tcdA +/tcdB + genotype of C. difficile and the emergence of resistance strains to broad-spectrum antibiotics and metronidazole. Key Words: Multidrug resistance, Clostridium difficile, Metronidazole 相似文献9.
Mohammadzadeh Sedigheh Milla Sylvain Ahmadifar Ehsan Karimi Masoumeh Dawood Mahmoud A. O. 《Fish physiology and biochemistry》2021,47(4):951-960
The use of recombinant gonadotropin-releasing hormone (rGnRH) has very rarely been tested in fish to promote spawning. This study evaluated the impact of recombinant chicken gonadotropin-releasing hormone (rcGnRH) with metoclopramide on the release of sex steroids and final maturation induction in goldfish (Carassius auratus) broodstock. For this purpose, goldfish broodstock was divided into four groups and treated with 0.9% NaCl with 20 mg/kg metoclopramide (Met) (C); 10 μg/kg body weight (BW) rcGnRH with 20 mg/kg metoclopramide (rcGn10); 15 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn15); and 20 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn20). The capability of the rcGnRH for eliciting biological response was tested in vivo by evaluating the changes of 17β estradiol (E2), testosterone (T), and 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) and the induced spawning. Blood samples were obtained at 0 h, 12 h, and 24 h after injection. The rcGn10, rcGn15, and rcGn20 treatments induced lower E2 concentration, especially 24 h post-injection. T levels were significantly higher in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection than at 0 h and then decreased at 24 h post-injection. Furthermore, the rcGnRH tested significantly enhanced DHP secretion in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection before a decline at 24 h post-injection. No significant difference between the sampling times was found in the C treatment for the 3 sex steroids tested. The results also displayed that rcGnRH at 10–20 µg/kg of body weight can trigger spawning with the highest speed and efficiency of spawning at 20 µg/kg. The obtained results represent a possible strategy for enhancing the artificial reproduction and ovulation of broodstock fish by rGnRH and further support the use of recombinant hormones to promote reproduction in aquaculture.
相似文献10.
Effect of Bacillus subtilis on Aeromonas hydrophila infection resistance in juvenile freshwater prawn, Macrobrachium rosenbergii (de Man) 总被引：1，自引：1，他引：0
This experiment was carried out to investigate the potential probiotics properties of Bacillus subtilis in protecting of juvenile freshwater prawn, Macrobrachium rosenbergii against Aeromonas hydrophila infection. A B. subtilis bacterium isolated from gut of juvenile prawns was added to the prawns feed at 108 cells g?1 feed. There were significant differences (P < 0.05) between B. subtilis-treated and control groups in growth and survival enhancement of juvenile prawns after 60 days of feeding trial. Sixty days after B. subtilis feeding trial, the prawns were challenged by bath exposure to A. hydrophila (107 cells mL?1) for 28 days. Four weeks after challenge, there was significant difference in the survival of prawns between B. subtilis-treated groups (88.33 %) and control groups (20.81 %; P < 0.05). In addition, the control groups had an unhealthy external appearance, while the treated groups, appeared healthy and normal. From this, it was concluded that the selected B. subtilis may be a promising probiotics for protection of prawns from A. hydrophila infection. 相似文献