Serologic and fecal markers to predict response to induction therapy in dogs with idiopathic inflammatory bowel disease          下载免费PDF全文

Cristiane C. Otoni  Romy M. Heilmann  Mercedes García‐Sancho  Angel Sainz  Mark R. Ackermann  Jan S. Suchodolski  Jörg M. Steiner  Albert E. Jergens 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):999-1008

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Genetic analysis of Aequipecten opercularis and Mimachlamys varia (Bivalvia: Pectinidae) in several Atlantic and Mediterranean localities, revealed by mitochondrial PCR-RFLPs: a preliminary study     

Mercedes Fernandez-Moreno  Alberto Arias-Perez  Ruth Freire  & Josefina Méndez 《Aquaculture Research》2008,39(5):474-481

Aequipecten opercularis (Queen scallop) and Mimachlamys (Chlamys) varia (Black scallop) are important natural resources occurring in Atlantic and Mediterranean coasts. To develop an optimal sustainable exploitation plan, it is important to study the genetic structure of the different populations. In this study, we used polymerase chain reaction‐restriction fragment length polymorphisms for the determination of the genetic variation and population structure of these two species in different localities. Ten composite haplotypes were generated for A. opercularis and 15 haplotypes for M. varia. Of these, six and four were unique respectively. The analysis of the distribution of the different haplotypes between the localities showed no clear evidence of subdivision in A. opercularis, while in M. varia the results indicated that the two localities analysed should be managed as separate stocks.  相似文献   

Effects of formalin on total haemocytes count and histopathological changes in the shrimp Litopenaeus schmitti (Pérez‐Farfante & Kensley 1997)     

Raico E Laria Lamela  Yanis Cruz Quintana  Raquel Silveira Coffigny  Mercedes Martínez  Norma González Herrate 《Aquaculture Research》2008,39(12):1316-1321

Formalin is used in shrimp industry to eliminate ectoparasites and as a disinfectant. However, the effect of formalin on shrimp's defense mechanism is unknown. Several biomarkers were used to assess the immune response of juveniles of the shrimp Litopenaeus schmitti exposed to different concentrations of formalin (10, 25 and 50 mg L^?1) during 24 and 48 h. Formalin concentrations of 25 and 50 mg L^?1 produced a significant reduction in the total haemocyte count at both 24 and 48 h. Peroxidase and phenoloxidase activity did not show variations. The total protein concentration in haemolymph of shrimps was not significantly different between experimental groups. Histological analysis showed hyperplasia in the gills of animals exposed at 10 mg L^?1 for 24 h; however, the exposure at 25 and 50 mg L^?1 caused necrosis in gills, hepathopancreas and the antennal gland.  相似文献   

Characterization of selected Spanish table wine samples according to their biogenic amine content from liquid chromatographic determination     

Romero R  Sánchez-Viñas M  Gázquez D  Bagur MG 《Journal of agricultural and food chemistry》2002,50(16):4713-4717

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Marine Anticancer Agents: An Overview with a Particular Focus on Their Chemical Classes     

Marilia Barreca  Virginia Span  Alessandra Montalbano  Mercedes Cueto  Ana R. Díaz Marrero  Irem Deniz  Ayegül Erdoan  Lada Luki&#x; Bilela  Corentin Moulin  Elisabeth Taffin-de-Givenchy  Filippo Spriano  Giuseppe Perale  Mohamed Mehiri  Ana Rotter  Olivier P. Thomas  Paola Barraja  Susana P. Gaudêncio  Francesco Bertoni 《Marine drugs》2020,18(12)

The marine environment is a rich source of biologically active molecules for the treatment of human diseases, especially cancer. The adaptation to unique environmental conditions led marine organisms to evolve different pathways than their terrestrial counterparts, thus producing unique chemicals with a broad diversity and complexity. So far, more than 36,000 compounds have been isolated from marine micro- and macro-organisms including but not limited to fungi, bacteria, microalgae, macroalgae, sponges, corals, mollusks and tunicates, with hundreds of new marine natural products (MNPs) being discovered every year. Marine-based pharmaceuticals have started to impact modern pharmacology and different anti-cancer drugs derived from marine compounds have been approved for clinical use, such as: cytarabine, vidarabine, nelarabine (prodrug of ara-G), fludarabine phosphate (pro-drug of ara-A), trabectedin, eribulin mesylate, brentuximab vedotin, polatuzumab vedotin, enfortumab vedotin, belantamab mafodotin, plitidepsin, and lurbinectedin. This review focuses on the bioactive molecules derived from the marine environment with anticancer activity, discussing their families, origin, structural features and therapeutic use.  相似文献   

Molecular characterization of microalgae used in aquaculture with biotechnology potential     

Mercedes Alonso  Fátima C. Lago  Juan M. Vieites  Montserrat Espi?eira 《Aquaculture International》2012,20(5):847-857

Microalgae are the main component of first tropic level in aquatic food chain; it is for this reason that they are used as food in aquaculture. Also due to its biotechnological potential properties, they are used in the production of diverse components, dyes, antioxidants, enzymes, emulsifiers, etc. The extended ways of microalgae applications require physiologically and genetically stable cultures as well as correctly identified organisms to guarantee reproducibility and reliability. But the variety of species and the morphological similarity between some of them make difficult the identification of some microalgae. The use of molecular markers has supplied a very useful tool for identification of microalgae in fast mode, such as in classification. The present study has worked on the molecular characterization of main species of microalgae used in aquaculture in base of the molecular markers 18S rRNA and 16S rRNA. Microalgae DNA has been amplified and sequenced, and the resultant sequences were analyzed and reflected in phylogenetic trees. The phylogenetic analyses obtained reflect as both molecular markers allow to differentiate the main genus used in aquaculture.  相似文献   

Effect of Fucoidan on the Mitochondrial Membrane Potential (ΔΨm) of Leukocytes from Patients with Active COVID-19 and Subjects That Recovered from SARS-CoV-2 Infection     

Karina Janice Guadalupe Díaz-Resendiz  Carlos Eduardo Covantes-Rosales  Alma Betsaida Benítez-Trinidad  Migdalia Sarahy Navidad-Murrieta  Francisco Fabian Razura-Carmona  Christian Daniel Carrillo-Cruz  Edwin Jaime Frias-Delgadillo  Daniela Alejandra Prez-Díaz  Matxil Violeta Díaz-Benavides  Mercedes Zambrano-Soria  Guadalupe Herminia Ventura-Ramn  Aurelio Romero-Castro  David Alam-Escamilla  Manuel Ivn Girn-Prez 《Marine drugs》2022,20(2)

Fucoidan is a polysaccharide obtained from marine brown algae, with anti-inflammatory, anti-viral, and immune-enhancing properties, thus, fucoidan may be used as an alternative treatment (complementary to prescribed medical therapy) for COVID-19 recovery. This work aimed to determine the ex-vivo effects of treatment with fucoidan (20 µg/mL) on mitochondrial membrane potential (ΔΨm, using a cationic cyanine dye, 3,3′-dihexyloxacarbocyanine iodide (DiOC₆(3)) on human peripheral blood mononuclear cells (HPBMC) isolated from healthy control (HC) subjects, COVID-19 patients (C-19), and subjects that recently recovered from COVID-19 (R1, 40 ± 13 days after infection). In addition, ex-vivo treatment with fucoidan (20 and 50 µg/mL) was evaluated on ΔΨm loss induced by carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 150 µM) in HPBMC isolated from healthy subjects (H) and recovered subjects at 11 months post-COVID-19 (R2, 335 ± 20 days after infection). Data indicate that SARS-CoV-2 infection induces HPBMC loss of ΔΨm, even 11 months after infection, however, fucoidan promotes recovery of ΔΨm in PBMCs from COVID-19 recovered subjects. Therefore, fucoidan may be a potential treatment to diminish long-term sequelae from COVID-19, using mitochondria as a therapeutic target for the recovery of cellular homeostasis.  相似文献   

Pseudoallencotyla gen. n., a new genus for Allencotyla pricei (Kritsky,Noble et Moser, 1978) (Monogenea: Heteraxinidae), with a key to genera of Heteraxininae     

Montero FE  Aznar FJ  Fernández M  Raga JA 《Folia parasitologica》2003,50(1):43-47

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Detection of anti-Leishmania infantum antibodies in sylvatic lagomorphs from an epidemic area of Madrid using the indirect immunofluorescence antibody test     

Inmaculada Moreno  Julio Álvarez  Nerea García  Santiago de la Fuente  Irene Martínez  Eloy Marino  Alfredo Toraño  Joaquin Goyache  Felipe Vilas  Lucas Domínguez  Mercedes Domínguez 《Veterinary parasitology》2014,199(3-4):264-267

An outbreak of human leishmaniosis was confirmed in the southwest of the province of Madrid, Spain, between July 2009 and December 2012. Incidence of Leishmania infection in dogs was unchanged in this period, prompting a search for alternative sylvatic infection reservoirs. We evaluated exposure to Leishmania in serum samples from animals in the area with an indirect immunofluorescence test (IFAT). Using promastigotes from six culture passages and a 1/25 threshold titer, we found anti-Leishmania infantum seroreactivity in 9.3% of cats (4 of 43), 45.7% of rabbits (16/35) and 74.1% of hares (63/85). Use of promastigotes from >10 in vitro passages resulted in a notably IFAT lower titer, suggesting antigenic changes during extended culture. Postmortem inspection of seropositive animals showed no clinical signs of infection. The results clearly suggest that asymptomatic hares were the main reservoir in the outbreak, and corroborate IFAT as a sensitive serological surveillance method to detect such cryptic Leishmania infections.  相似文献   

Neospora caninum infection as a cause of reproductive failure in a sheep flock     

Marta González-Warleta  José Antonio Castro-Hermida  Javier Regidor-Cerrillo  Julio Benavides  Gema álvarez-García  Miguel Fuertes  Luis Miguel Ortega-Mora  Mercedes Mezo 《Veterinary research》2014,45(1)

Neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. This study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a commercial sheep farm. Sera from all rams and ewes tested negative for antibodies against Border disease virus, Schmallenberg virus and Coxiella burnetii, and infections by these agents were therefore ruled out. Nevertheless, seropositivity to N. caninum and/or Toxoplasma gondii was detected, although the seroprevalence was higher in the case of N. caninum. The percentage of lambings and the number of lambs per dam were significantly lower in ewes that were seropositive to N. caninum while no effect on these parameters was detected in ewes that were seropositive to T. gondii. There was also no evidence of infection by T. gondii in the foetal/lamb tissues analyzed by PCR and/or immunohistopathological techniques. On the contrary, the DNA of N. caninum was detected in 13 out of 14 foetuses/lambs descendant from dams seropositive to this parasite. Characteristic lesions caused by N. caninum and/or its antigen were also detected. Genotyping of the N. caninum DNA revealed only two closely related microsatellite multilocus genotypes. The results clearly demonstrate that infection by N. caninum was the cause of the low reproductive performance of this sheep flock.  相似文献