首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   135篇
  免费   7篇
林业   8篇
农学   17篇
基础科学   3篇
  29篇
综合类   13篇
农作物   4篇
水产渔业   16篇
畜牧兽医   39篇
园艺   3篇
植物保护   10篇
  2023年   1篇
  2022年   1篇
  2021年   4篇
  2020年   3篇
  2019年   5篇
  2018年   3篇
  2017年   4篇
  2016年   4篇
  2015年   10篇
  2014年   2篇
  2013年   15篇
  2012年   6篇
  2011年   4篇
  2010年   3篇
  2009年   2篇
  2008年   7篇
  2007年   3篇
  2006年   4篇
  2005年   1篇
  2003年   7篇
  2002年   8篇
  2001年   2篇
  2000年   6篇
  1999年   3篇
  1998年   2篇
  1997年   2篇
  1996年   2篇
  1995年   4篇
  1994年   1篇
  1993年   1篇
  1992年   1篇
  1991年   1篇
  1989年   2篇
  1988年   1篇
  1986年   1篇
  1984年   2篇
  1983年   1篇
  1982年   1篇
  1981年   1篇
  1976年   2篇
  1975年   1篇
  1974年   1篇
  1972年   1篇
  1971年   1篇
  1968年   2篇
  1967年   1篇
  1962年   1篇
  1958年   1篇
排序方式: 共有142条查询结果,搜索用时 31 毫秒
1.
We evaluated the effects of the addition of two types of spent mushroom substrate (SMS), SMS from an Agaricus bisporus crop (SMS1) and a mixture of SMSs from an A. bisporus crop and a Pleurotus crop (50% v/v each) (SMS2), on the characteristics of a calcareous clayey‐loam soil and the yield and nutritional status of lettuce (Lactuca sativa L.), relative to crops receiving mineral fertilizer (M) and a control (C) without amendment. The application of SMS, especially SMS1, improved soil fertility compared with C and M soils. Moreover, the use of these organic substrates as soil amendments did not harm the plants and gave yields similar to that obtained with mineral fertilizer. The nutritional contents of the lettuce plants were greater than or similar to those of the plants from treatment C or M, except for the plant tissue concentrations of K, Fe and Zn, which were significantly reduced by SMS application. However, this latter fact did not reduce the lettuce yield in the amended soils. Therefore, the use of SMSs as organic amendments contributes to residue utilization, in an environmentally friendly way, and to improved soil fertility and crop yield.  相似文献   
2.
3.
Nixtamalization is an ancient process developed by the Mesoamerican cultures. Initially, volcanic ashes were used and then calcium hydroxide in commercial production, and more recently nixtamalization with calcium salts (NCS) has been proposed. The aim of this study was to evaluate the effect of NCS on carbohydrate digestibility and antioxidant capacity in the elaboration of blue maize tortillas. NCS in blue tortillas showed a high amount of total dietary fiber (14.27 g/100 g), the main fraction being insoluble dietary fiber. The contents of resistant starch and slowly digestible starch did not change with the nixtamalization process. The predicted glycemic index value was lower in blue tortillas with the NCS process (58) than with the traditional nixtamalization process (71). In general, NCS in blue tortillas presented a higher antioxidant capacity than traditional tortillas (ferric reducing antioxidant power method), indicating that phenolics present in blue maize maintain their activity after cooking. It can be concluded that the nutraceutical features (high dietary fiber content and antioxidant capacity) of blue maize tortillas are enhanced when they are elaborated with the NCS process.  相似文献   
4.
Transplantation of ovarian tissue has high potential for female gamete conservation. However, optimal timing of oocyte recovery for in vitro maturation and fertilization is still critical. Therefore the aim of the present study was to use high-resolution transcutaneous ultrasonography to monitor follicular development within xenografted ovarian tissue. Ovarian cortex fragments (n=44) from domestic cats were transplanted into athymic nude rats (n=12). Graft development in the animals was assessed weekly by high frequency ultrasound (10-22 MHz) under two different FSH regimes. Blood collection for serum estradiol determination and vaginal smears were performed simultaneously. The xenografts were removed at different time points according to the ultrasound findings. The survival rate of the transplants 4 weeks after surgery was 54.5% and antral follicular growth was observed within 10 grafts from 5 different hosts (8.6 +/- 6.43 follicles per graft). Early follicle antrums could be detected from 0.4 mm onwards. The growth rate of the antral cavity was calculated from weekly measurements (0.56 +/- 0.44 mm per week). Although vaginal cells and estradiol levels followed a cyclic pattern, no correlation was found between follicular diameter, estradiol and keratinized vaginal cells. We recovered 5, 1 and 4 cumulus oocyte complexes from three different individuals during weeks 19, 21, and 23 respectively. Extrusion of a polar body (1 oocyte) and germinal vesicle break down (7 oocytes) indicated progression of maturation after in vitro culture. We conclude that ultrasonography und provided a reliable method to examine xenograft survival and follicular development within the grafts. Furthermore, this technique is suitable for assessment of the efficiency of hormonal treatment and narrowing of the optimal time frame for oocyte retrieval. To our knowledge, this is the first report of the in vivo development of early antral follicles in mammalian species.  相似文献   
5.
Paris  A.  Bonardi  S.  Bacci  C.  Boni  E.  Salmi  F.  Bassi  L.  Brindani  F. 《Veterinary research communications》2010,34(1):145-148

The aim of the study was to evaluate the sensitivity of two m-PCR methods for the quantitative determination of E. coli O157:H7 in foodstuffs. Genomic serotyping was carried out on bacterial cultures, and the necessary time was optimized to increase the resolution of the method. Subsequently, artificial contamination trials using meat were conducted to assess method accuracy in foodstuffs and pursue the genetic typing of pathogens. Measurement thresholds were shown to range between 105 and 106 CFU/mL, but were reduced by four logarithmic cycles in 80% of samples. Relative to the meat contamination trials, serotypes were identified after 24 hours, corresponding to 10 CFU/mL inoculum, with higher rates seen when m-TSB was used for enrichment. Inoculated samples were found to contain three virulence factors (hlyA, eaeA, and stx1).

  相似文献   
6.
In October 2004, 41 goats > 2 years old from a Saanen dairy goat herd located in Purranque County, 10th Region, Chile, were sampled and tested for paratuberculosis. While collecting samples it was observed that several goats were thin and emaciated. One goat was sufficiently debilitated to warrant humane euthanasia. This animal was brought to the Veterinary School at the Universidad Austral de Chile for necropsy. The goat selected for necropsy was a 12-year-old doe. The animal showed classical clinical signs of caprine paratuberculosis: emaciation despite willingness to eat, dry and rough hair coat, and no evidence of diarrhea. Gross pathology and histopathology of the necropsied goat were consistent with paucibacillary paratuberculosis. Bacteriology, serology, and PCR confirmed the diagnosis. This is the first published report of goat paratuberculosis in Chile confirming a case of caprine paucibacillary paratuberculosis.  相似文献   
7.
In Italy, new agroforestry cultural models could play an important role in the diffusion of plantation forestry timber species. We studied the stem growth rates and leaf water potentials () of common walnut (Juglans regia L.), and gravimetric soil moisture (DW) depletion during the third and fourth growing seasons in an agroforestry trial in central Italy. Since the establishment of experimental plots in 1992, walnut was intercropped with alfalfa (Medicago sativa L.), with or without polyethylene (PE) mulching along tree rows. By the end of the study period, the unmulched-intercropped walnut was almost 68% smaller in stem diameter and height than the sole-unmulched control, demonstrating walnuts sensitivity to alfalfa competition. The competitive effect of alfalfa on walnut stem growth was effectively controlled/reduced by PE mulching. Stem growth rates of mulched-intercropped walnut were always lower than the control, but much higher (ca. 235% in stem diameter and height) than those of unmulched-intercropped walnut. The higher field performance of mulched-intercropped walnut compared to the un- mulched-intercropped treatment was associated with higher soil moisture and predawn and midday values during the summers driest periods. The presence of PE mulching was also associated with lower levels of soil moisture during the early and late growing season, but this did not negatively affect walnut and growth rates. These results suggest that in the climatic conditions of central Italy, competition for soil moisture between young common walnut trees and alfalfa can be substantially lowered, but not completely eliminated, by PE mulching.This revised version was published online in November 2005 with corrections to the Cover Date.  相似文献   
8.
Fady  B.  Ducci  F.  Aleta  N.  Becquey  J.  Diaz Vazquez  R.  Fernandez Lopez  F.  Jay-Allemand  C.  Lefèvre  F.  Ninot  A.  Panetsos  K.  Paris  P.  Pisanelli  A.  Rumpf  H. 《New Forests》2003,25(3):211-225
Adaptive and wood quality trait data were collected and analyzed on commercially available Juglans regia and J. regia×J. nigra provenances and progenies planted across Europe in a multi-site network. A total of 19 seed sources, replicated 35 times per site, were planted at 13 sites from 5 European countries, encompassing the potential distribution area of timber production plantation sites. The following traits were evaluated: survival, height, diameter at breast height, stem form, apical dominance, vegetative budbreak, along with biotic and abiotic damage. Mean values were significantly different both among provenances/progenies and sites. Most common damage was late spring and early autumn frost. Bud break ranking was significantly correlated with provenance and progeny origin. Although J. regia is fast growing, southern European early budbreak plant material should not be planted under most middle European conditions where late spring frost can be expected, as it has a significant negative impact on architectural (and thus wood quality) traits. Hybrid J. regia×J. nigra progeny performed better than J. regia provenances/progenies for most traits measured. Differences were significantly in favor of hybrids at sites with medium to low fertility, although some locally selected seed sources tended to perform as well as hybrids on high fertility sites.  相似文献   
9.
Combined production of timber and forage species is considered to be a desirable land-use system to offset some of the current land management problems of Italy. This paper reports the early results of a study on intercropping young plants of walnut with alfalfa (Medicago sativa L.), with or without polyethylene mulching, in an experimental plantation in central Italy. Two genotypes of walnut were used:Juglans regia L. and the French hybrid NG23×RA. The seasonal changes of absolute and relative growth rates of walnut stem diameter and apical shoot length were investigated.During both growing seasons, alfalfa intercropping strongly decreased walnut growth, whereas polyethylene mulching promoted stem height and diameter growth. Stem growth of intercropped-and-mulched walnut was the same as that of sole walnut under clean cultivation management. The use of polyethylene mulching reduced or avoided the competitive effect of associated alfalfa on walnut growth. During both growing seasons, the intercropped-and-mulched walnut had higher relative growth rates than intercropped-unmulched walnut. The results show the effectiveness of polyethylene mulching in promoting early growth of walnut, even when intercropped with alfalfa during early stages of plantation establishment.  相似文献   
10.
The effect of GH treatment on hepatic cytochrome P450 content, aryl hydrocarbon hydroxylase (AHH), aminopyrine-N-demethylase (AND), testosterone hydroxylase, testosterone 5- and 5-reductase, UDP-glucuronyl transferase (UDPGT) and glutathione S-transferase (GST) activities in immature rainbow trout were investigated. Hepatic cytochrome P450 content, AHH and GST activities were measured in both GH implanted and GH injected animals whereas other activities were assayed in GH implanted trout only.GH implants significantly decreased cytochrome P450 content at 15 days compared to the control but no significant effect was observed at 15 or 30 d when GH was injected biweekly. In both cases, AHH activity was significantly decreased by GH treatment compared to the control whereas GST remained unchanged. Compared to the control, GH implanted fish exhibited a pronounced inhibition of AND, a decreased 6 and 16-testosterone hydroxylation, an inhibition of UDPGT with testosterone as substrate and an enhanced 17-testosterone oxidation.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号