Stimulation of ROS-scavenging systems in squash (Cucurbita pepo L.) plants by compost supplementation under normal and low temperature conditions     

Kamel A.H. Tartoura  Sahar A. Youssef 《Scientia Horticulturae》2011

The beneficial effect of compost, the final product of aerobic biodegradation of organic matter, on growth, lipid peroxidation [as malondialdehyde (MDA], hydrogen peroxide (H₂O₂) and superoxide anion (O₂^•−), activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR), as well as reduced ascorbate (ASC) and glutathione (GSH) and their oxidized forms was investigated in squash (Cucurbita pepo L. cv. Eskandarany) plants exposed to normal and low temperature (LT) conditions. LT stress of 8 °C significantly reduced the plant growth of untreated plants, but compost alleviated the adverse effect of stress and significantly increased the fresh and dry weights under normal and stress conditions. LT also induced accumulation of H₂O₂ and O₂^•− and resulted in increased lipid peroxidation, pointing out to cellular oxidative stress. Under compost application, such reactive oxygen species (ROS) and peroxidized lipids were markedly reduced, but SOD, CAT, APX and GR activities, key enzymes of ROS-scavenging systems, were significantly increased. Data also indicated that there were general reductions in total ascorbate and glutathione pool in LT control plants, but compost-treated ones considerably have maintained higher levels of such redox metabolites. Significantly higher ratios of ASC/DHA (dehydroascorbate) and GSH/GSSG (glutathione disulfide) were generally found in compost-treated plants than in untreated-ones. It is evident that compost induced enhancement of LT tolerance was related to up-regulation of enzymatic and non-enzymatic antioxidant systems. Such enhancement would eventually protect plant cells from LT-induced oxidative stress reactions via scavenging ROS.  相似文献   

Serological diagnosis of bluetongue by blocking or competitive ELISA by four laboratories.     

A Afshar  J Anderson  B T Eaton  G A Gustafson 《Journal of veterinary diagnostic investigation》1991,3(3):255-257

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Experimental Infection of Goats with Parainfluenza Virus Type 3     

A. Afshar  S. Terlecki 《Zoonoses and public health》1979,26(8):641-651

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Immunoperoxidase plaque staining for the detection of pseudorabies virus.   总被引：1，自引：0，他引：1       下载免费PDF全文

A Afshar  G C Dulac 《Canadian journal of veterinary research》1986,50(1):118-119

A quantitative indirect immunoperoxidase plaque staining method was developed for the detection of pseudorabies virus infection in a pig kidney cell line (PK-15). The method is rapid and specific and foci of infection, represented by stained plaques, are easily counted by the unaided eye. Possible modification of this technique in a plaque reduction assay for the detection of antipseudorabies virus antibody is also discussed.  相似文献   

Development and evaluation of an indirect enzyme immunoassay for detection of porcine antibodies to pseudorabies virus.   总被引：3，自引：1，他引：2       下载免费PDF全文

A Afshar  P F Wright    G C Dulac 《Canadian journal of veterinary research》1986,50(3):422-426

An indirect enzyme immunoassay is described for detection of porcine serum antibody to pseudorabies virus. The analytical sensitivity of the enzyme immunoassay was found to be approximately 4.5 log 4 X 10 (5120 times) greater than the serum neutralization test, based on parallel end point titrations. The diagnostic sensitivity of the enzyme immunoassay was comparable or superior to that of the serum neutralization, based on the earliest detectable antibody after infection of swine with pseudorabies virus by intranasal or intrauterine routes or by contact with infected pigs. The enzyme immunoassay, at a screening dilution of 1:20, gave 100% agreement with ELISA results provided with a U.S. Department of Agriculture-Animal and Plant Health Inspection Service proficiency panel of 40 sera. One serum having demonstrable antibody by the enzyme immunoassay was seronegative by the serum neutralization test.  相似文献   

The occurrence of precipitating antibodies to bovine adenovirus in sera of farm animals and man in Iran   总被引：1，自引：0，他引：1  

A Afshar 《The Veterinary record》1969,84(23):571-572

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Occurrence of precipitating antibodies to bluetongue virus in sera of farm animals in Iran     

A Afshar  H Kayvanfar 《The Veterinary record》1974,94(11):233-235

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Combining ability and gene action for maturity and agronomic traits in different heterotic groups of maize inbred lines and their diallel crosses   总被引：1，自引：0，他引：1  

Afshar. Estakhr  Bahram. Heidari 《Journal of Crop Science and Biotechnology》2012,15(3):219-229

Combining ability information is necessary for selection of suitable advanced lines for hybridization and identification of promising hybrids for development of improved varieties. A number of 14 maize (Zea mays L.) inbred lines and 91 related crosses were evaluated over two years, 2008 and 2009, in a temperate-zone of Iran. The objectives of the study were to identify the best general and specific combiners, heterosis and type of gene actions responsible for agronomic traits. Except for grain yield and growing degree day to milky, significant general (GCA) and specific (SCA) combining ability were observed for all traits. The Baker ratio for plant height (0.15), ear height (0.26), growing degree day to milky stage (0.04), and grain yield (0.002) showed the predominance of non-additive gene effects in the expression of these traits. The heterosis observed for grain yield, grain number, pollination period, ear and plant height was considerably higher than that observed for other traits. The correlations (r) of F₁ means and SCA effects were positive and significantly higher than that of r (F₁, mid-parents) and r (F₁, heterosis) for all the traits except cob percent, growing degree days to silking, and physiological maturity. MO17, K3547/5, and K3615/2 had negative GCA effects for growing degree day to milky stage and maturity. Among hybrids, MO17 × K3653/2, B73 × K3651/2, and K3545/6 × K3493/1 with positive SCAs for pollination period and grain yield had also negative SCA effects for degree day to silking and milky stages. Therefore, the use of these inbred lines and hybrids increases the response to selection for increasing grain yield and early maturity in maize.  相似文献   

A novel attempt at artificial spawning of captive bonefish (Albula spp.)     

Sahar Mejri  William R. Halstead  Cameron A. Luck  Christopher Robinson  Travis E. Van Leeuwen  Aaron J. Adams  Jon Shenker  Matthew J. Ajemian  Paul S. Wills 《Aquaculture Research》2019,50(9):2718-2723

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Production of an Antibody Fragment (scFv) Targeting PcrV Protein of Pseudomonas aeruginosa in Fed-Batch Cultivation Mode     

Saba Karam  Mozhgan Raigani  Sahar Hassani Afshar  Yeganeh Talebkhan  Elham Bayat  Samira Komijani  Leila Nematollahi  Farzaneh Barkhordari  Mehdi Shafiee Ardestani  Fatemeh Davami 《Iranian Biomedical Journal》2021,25(6):390

Background: Pseudomonas aeruginosa is one of the opportunistic pathogens causing frequent hospital-acquired life-threatening infections in mechanically ventilated patients. The most significant virulence factor of P. aeruginosa is T3SS. PcrV is an important structural protein of the T3SS. Methods:In the current investigation, a recombinant scFv mAb against the PcrV protein was expressed in EnBase® (fed-batch) cultivation mode. The pETiteTM N-His SUMO Kan vector, including anti-PcrV scFv gene, was transformed into Escherichia coli (BL21) cells. The expression and solubility of anti-PcrV scFv protein were investigated at two different temperatures (25 °C and 30 °C) and at different induction times (4, 6, 8, 12, and 24 hours). Results:Increased efficiency was achieved by EnBase® compared to LB broth; owing to the slow release of glucose, the maximum level of solubility and total protein expression was observed in EnBase® cultivation system at 30 °C and 24 h post induction. Furthermore, IC₅₀ for anti-PcrV scFv protein was determined to be approximately 7 μg/mL. Conclusion:Anti-PcrV scFv produced in this study showed promising in vitro results, protecting RBC from lysis by P. aeruginosa (exoU+). Key Words: Fed Batch, recombinant protein, Pseudomonas aeruginosa, scFv  相似文献