Indole-3-acetic acid biosynthesis in <Emphasis Type="Italic">Aciculosporium take</Emphasis>, a causal agent of witches' broom of bamboo     

Eiji Tanaka  Chihiro Tanaka  Atsushi Ishihara  Yasumasa Kuwahara  Mitsuya Tsuda 《Journal of General Plant Pathology》2003,69(1):1-6

 Aciculosporium take (Ascomycota; Clavicipitaceae) is a causal agent of witches' broom of bamboo plants. The symptoms of this disease are believed to be induced by plant hormones, particularly auxins. Indole-3-acetic acid (IAA) was identified in cultures of this fungus in an l-tryptophan-supplemented liquid medium. IAA production was confirmed on 30 isolates of A. take from various hosts and locations at levels up to 1 mg/l. The biosynthetic pathway of IAA in A. take culture was examined by analyzing intermediate products and by feeding experiments. The results showed that the indole-3-pyruvic acid pathway (l-tryptophan → indole-3-pyruvic acid → indole acetaldehyde → IAA) was the dominant pathway in A. take. Received: June 3, 2002 / Accepted: July 25, 2002  相似文献   

Tear ferning test in horses and its correlation with ocular surface evaluation          下载免费PDF全文

Laura R. Silva  Anderson F. Gouveia  Cleyber J. T. de Fátima  Letícia B. Oliveira  Janildo L. Reis Jr.  Renato F. Ferreira  Concepta M. Pimentel  Paula D. Galera 《Veterinary ophthalmology》2016,19(2):117-123

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Spindle formation and microtubule organization during first division in reconstructed rat embryos produced by somatic cell nuclear transfer     

Tomioka I  Mizutani E  Yoshida T  Sugawara A  Inai K  Sasada H  Sato E 《The Journal of reproduction and development》2007,53(4):835-842

The present study was conducted to demonstrate the spindle formation and behavior of chromosomes and microtubules during first division in reconstructed rat embryos produced by somatic cell nuclear transfer (SCNT) with cumulus cell nuclei. To demonstrate the effect of oocyte aging after ovulation on the cleavage of SCNT embryos, micromanipulation was carried out 11, 15 and 18 h after injection of hCG. SCNT oocytes were activated by incubation in culture medium supplemented with 5 microM ionomycin for 5 min followed by treatment with 2 mM 6-dimethylaminopurine (6-DMAP) in mR1ECM for 2-3 h. For immunocytochemical observation, the SCNT embryos were incubated with monoclonal anti-alpha-tubulin antibody and then fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG. Cleavage rates were significantly higher for oocytes collected after 15 and 18 h rather than for those collected 11 h after injection of hCG (56 and 53%, respectively vs. 28%; P<0.05). Premature chromosome condensation occurred before activation of the SCNT oocytes, but adequate spindle formation was only rarely observed. The distribution of microtubules in SCNT embryos after activation was different from those of fertilized and parthenogenic oocytes, i.e., a dense microtubule organization shaped like a ring was observed. Eighteen to 20 h post-activation, most SCNT embryos were in the 2-cell stage, but no nucleoli were clearly visible, which was quite different from the fertilized oocytes. In addition, first division with and without small cellular bodies containing DNA was observed in the rat SCNT embryos in some cases. The present study suggests that reorganization of transferred nuclei in rat SCNT embryos may be inadequate in terms of formation of the mitotic assembly and nucleolar reorganization.  相似文献   

Ineffectiveness of applying additional insecticide sprays at the border of the citrus block for the control of Huanglongbing     

Isabela Vescove Primiano  Fabiano Parra Asato  Renato Beozzo Bassanezi 《Pest management science》2023,79(3):1113-1122

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Canine squamous cell carcinoma: Electrochemotherapy association with surgery and correlation with overall survival     

Rúbia Monteiro de Castro Cunha  Gleidice Eunice Lavalle  Fernanda Camargo Nunes  Ayisa Rodrigues de Oliveira  Renato de Lima Santos  Roberto Baracat de Araújo 《Veterinary and comparative oncology》2023,21(2):240-254

Squamous cell carcinoma (SCC) is an important malignancy in dogs, due to its incidence and clinical presentation, which can be of locally aggressive single or multiple lesions with a metastatic potential. The objective of this investigation was to evaluate SCC response to treatment, anatomopathological and immunohistochemical characteristics, disease-free interval and overall survival time. 54 dogs with histopathologically diagnosed SCC were included in this study. Their mean age was 9.16 years with a range of 1–14 years. Of the 54 animals in the study, 34 (65.4%) had white skin and white fur coats. There was a significant correlation between fur coat colour and the development of tumours in areas of sun exposure (p = .001). Animals with tumours in areas of the body exposed to the sun had longer overall survival time than animals with tumours in areas not associated with sun exposure (p = .001). Surgery combined with electrochemotherapy (ECT) yielded a survival rate 32% higher than using a surgical approach alone (HR = 0.32, p = .038, IC = 0.11–0.94). ECT, with or without surgery, had an objective response rate of 90.9%. Local lymph node and/or distant site metastasis at diagnosis, or at some point during follow-up, occurred in 34.6% (18/52) of animals. Animals with tumours in sun exposed locations had more aggressive histopathological characteristics but had longer overall survival time. This is probably due to individualised therapeutic treatment with both surgery and ECT.  相似文献   

Stock assessment of Japanese eels using Japanese abundance indices     

Eiji Tanaka 《Fisheries Science》2014,80(6):1129-1144

The paper compiles a catch history of Japanese eels Anguilla japonica in East Asia and some Japanese relative abundance series. Maximum likelihood estimates of stock abundance of eels have been obtained using the abundance series and various biological parameters, such as growth, maturity and natural mortality. Age- and sex-structured models have been used to express the dynamics of stock abundance, and the Beverton and Holt model has been used to express the relationship between stock and recruitment. Data for estimations are standardized catch per unit effort of commercial fishery for exploitable stock (1954–2006 and 1968–2008) and for glass eel (1954–2010, 1972–2004, and 1973–1997). From the results of the base case scenario of estimations, the estimated stock size of individuals aged ≥1 year was 18.7 thousand tons in 2010, which was 24 % of the carrying capacity. The estimated stock size has recovered since 1990. Maximum sustainable yield was 4,180 tons if only the exploitable stock were utilized, and 266 tons if only the glass eel were utilized. These results and issues relating to estimation and management for reducing the fishery impact on stock are discussed.  相似文献   

日本冲绳岛琉球松林台风干扰后的群落特点   总被引：3，自引：0，他引：3  

王勤徐小牛  平田永二 《安徽农业大学学报》2003,30(4):400-406

为阐述亚热带地区琉球松林受到干扰后的演替规律,就日本冲绳地区琉球松人工林在强台风干扰后,林分结构和树种组成特点进行了调查分析。结果表明,琉球松林经台风干扰后演替形成两种群落类型,即琉球松-峨嵋木荷群落和琉球松-峨嵋木荷-蕨类群落;两者在林分结构和树种组成特点方面差异明显。琉球松-峨嵋木荷群落中,出现乔灌树种26种(DBH≥3．0cm),树种多样性指数为2．55,林下植被特别是草本层盖度低,没有蕨类植物分布。而琉球松-峨嵋木荷-蕨类群落中,出现乔灌树种33种,树种多样性指数为3．51,林下植被特别是草本层发育较好,并有大量蕨类植物分布,其盖度达55％。此外,经受干扰的琉球松林出现了大量的较耐荫的常绿阔叶树种,尤其是山茶科和樟科的树种,而没有出现本地天然常绿阔叶林的优势树种壳斗科的栲类(Castanopsis spp.)。充分说明强台风干扰促进了琉球松林向常绿阔叶林演替。  相似文献   

Radiation-Induced Breeding in Camelina Sativa     

Chung  Yong Suk  Silva  Renato Rodrigues  Park  Minwoo  Lee  Sanghyeob 《Journal of Crop Science and Biotechnology》2019,22(1):17-20

Journal of Crop Science and Biotechnology - The ionizing radiation methods, including X-rays, gamma rays, and neutrons to create variation is well established and used in major crops. Many...  相似文献   

Plant species identification using fecal DNAs from red-eared slider and Reeves’ pond turtle in agricultural canals for rural ecosystem conservation     

Noriyuki Koizumi  Atsushi Mori  Takuya Mineta  Eiji Sawada  Keiji Watabe  Takeshi Takemura 《Paddy and Water Environment》2017,15(4):723-730

Fecal DNA samples from the red-eared slider and Reeves’ pond turtle, suspected pests of lotus root paddies, were used to identify the plant species eaten by these turtles in order to develop a strategy for rural ecosystem conservation. The fecal samples were obtained from young and adult individuals (mostly female) of both species living in agricultural canals surrounding lotus root paddies in Tokushima Prefecture, Japan. The samples were screened for the presence or absence of DNA from nine plant species using PCR and plant species-specific primers for the rbcL gene of chloroplast DNA. In the red-eared slider, our analysis identified seven plant species in the fecal DNA samples of adults and three plant species in those of young individuals. In Reeves’ pond turtle, our analysis identified two plant species from adult fecal samples and one species from those of young individuals. Thus, adult red-eared sliders consume a greater range of plants than young red-eared sliders or Reeves’ pond turtles. Both turtle species, independently of age, consumed lotus plants and were likely to cause feeding damage to lotus roots. Considering the plant species detected in adult red-eared sliders and these plant habitats, we suggest that this adult turtle is likely to travel between the agricultural canals and the lotus root paddies. These findings will help the development of strategies for preventing damage to lotus roots by these turtles; furthermore, they indicate that fecal DNA analysis will be applicable to investigation of the feeding habits of other animal species.  相似文献   

Infection of a chimeric simian and human immunodeficiency virus with CCR5-specific HIV-1 envelope to Rhesus macaques     

Enose Y  Miyake A  Ido E  Hayami M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):283-286

Human immunodeficiency virus (HIV) infects lymphocytes and macrophages via CD4 and chemokine receptors. In this study, the infectivity of a chimeric simian and human immunodeficiency virus (SHIV) having a CCR5-specific HIV-1 envelope gene was examined. A SHIV strain termed SHIV-JRFL could enter cells via CD4 with a chemokine receptor CCR5, not CXCR4, and the viral replication was suppressed by recombinant human RANTES, one of beta-chemokines. The intravenous inoculation of SHIV-JRFL into two rhesus macaques resulted in a systemic infection, though it was rather weak. During the early infection, the production of RANTES from Con A-stimulated PBMCs of the infected monkeys increased. These results suggested that beta-chemokine has the potential to limit the infectivity of an R5-type SHIV.  相似文献