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ABSTRACT: Changes in the freshness indices and extractive components in the foot muscle of live small abalone Haliotis diversicolor during storage at 5°C, 15°C, and 25°C were investigated. The pH values declined with storage time. Volatile basic nitrogen and the K -value increased gradually with storage time at 15°C and 25°C, but changes were small at 5°C. The onset of initial decomposition of samples was observed after 3.5 days at 5°C, after 2.5 days at 15°C, and after one day at 25°C. Adenosine triphosphate and adenosine diphosphate degraded rapidly within the early days of storage. In contrast, levels of adenosine monophosphate increased and exhibited prolonged accumulation throughout the storage period. The total amount of free amino acids increased markedly during storage. The dominant free amino acids, such as taurine, glutamic acid, glycine, alanine, and arginine, also increased after storage. 相似文献
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A feeding experiment was conducted to determine the dietary calcium (Ca) requirement for juvenile hybrid tilapia, Oreochromis niloticus × O. aureus reared in nature water. Purified diet supplemented with 0, 1, 2, 3, 4, 5, 7 and 10 g Ca kg−1 diet providing of 0.6, 1.6, 2.6, 3.7, 4.7, 5.5, 7.5 and 10.7 g Ca kg−1 diet, respectively, were fed to tilapia (mean initial weight: 0.52 ± 0.01 g, n = 3) for 8 weeks. Each diet was fed to three replicate groups of fish in a closed, recirculating fresh water rearing system. The rearing water contained 27.1–33.3 mg L−1 Ca. The tilapia fed the diets supplemented with ≥3.7 g Ca kg−1 had significantly (P < 0.05) higher weight gain, when compared with fish fed the diet with ≤1.6 g Ca kg−1. Fish fed the unsupplemented control showed significantly lower weight gain when compared with the other groups (P < 0.05). Bone Ca concentration was highest in fish fed the diets with ≥4.7 g Ca kg−1, intermediate in fish fed the diet with 2.6 g Ca kg−1 and lowest in fish fed the control diet. Scale Ca concentration was higher in fish fed the diets with ≥3.7 g Ca kg−1 than in fish fed the diets with ≤2.6 g Ca kg−1. Serum alkaline phosphatase activity was 36% increased in fish fed the diets with ≥2.6 g Ca kg−1 than fish fed the diets with <1.6 g Ca kg−1. Analysis by broken‐line regression of weight gain, bone and scale Ca concentrations indicated that the adequate dietary Ca concentration for tilapia in water containing 27.1–33.3 mg Ca L−1 was 3.5, 4.3 and 4.2 g Ca kg−1 diet, respectively, supplied as Ca‐lactate. 相似文献
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An 8‐week feeding trial was conducted to determine two vitamin C derivatives, l ‐ascorbyl‐2‐sulphate (C2S) and l ‐ascorbyl‐2‐polyphosphate (C2PP), to satisfy vitamin C requirement and to test their effects on the non‐specific humoral immune responses of juvenile grouper, Epinephelus malabaricus. C2S and C2PP were each supplemented at 20, 50, 80, 150, 250 and 400 mg kg?1 diet in the semi‐purified basal diet providing of 7, 16, 28, 55, 86, 142 mg ascorbic acid (AA) equivalent of C2S kg?1 diet and 4, 9, 15, 31, 49, 75 mg AA equivalent of C2PP kg?1 diet, respectively. Basal diet without AA supplemented was included as a control. Each diet was fed to triplicate groups of grouper (mean initial weight: 6.69 ± 0.07 g). Fish fed diets with ≥28 mg AA equivalent of C2S or ≥4 mg AA equivalent of C2PP kg?1 had significantly (P < 0.05) greater weight gain (WG) than fish fed the unsupplemented control diet. Liver AA concentrations were higher in fish fed diets with ≥16 mg AA equivalent of C2S or ≥9 mg AA equivalent of C2PP kg?1 than fish fed the control diet. Alternative pathway of complement activation (ACP) was higher in fish fed diets with ≥55 mg AA equivalent of C2S or ≥15 mg AA equivalent of C2PP kg?1 than fish fed the control diet. Lysozyme activity was higher in fish fed ≥86 mg AA equivalent of C2S or ≥15 mg AA equivalent of C2PP kg?1 than fish fed the control diet. Analysis by broken‐line regression of WG indicated that the adequate dietary vitamin C concentration from each vitamin C derivative in growing grouper is 46.2 mg AA equivalent of C2S kg?1 diet and 17.8 mg AA equivalent of C2PP kg?1 diet, and it also indicated that C2S is approximately 39% as effective as C2PP in meeting the vitamin C requirement for grouper. The data suggest that both C2S and C2PP supplementation support non‐specific immune responses of grouper. 相似文献
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Basal diet containing 0.5, 1.0, 1.5 and 2.0 g kg?1 mixture of inosine monophosphate (IMP), adenosine monophosphate (AMP), guanosine monophosphate (GMP), uridine monophosphate (UMP) and cytidine monophosphate (CMP) (1 : 1 : 1 : 1 : 1) (mixed‐NT; Experiment 1) and 1.5 g kg?1 from each nucleotides and mixed‐nucleotides (NT; Experiment 2) were fed to triplicate groups of grouper for 8 weeks. Basal diet without NT was used as control in both Experiments. In Experiment 1, fish fed the diet with 1.5 g mixed‐NT kg?1 had higher (P < 0.05) weight gain (WG) than the control group. The superoxide anion (O2?) production ratio was higher in fish fed diets with 1.0–1.5 g mixed‐NT kg?1 than the fish fed diets with ≤0.5 g mixed‐NT kg?1. In Experiment 2, fish fed diets with nucleotides had higher WG than the control group. The O2? production ratio was higher in fish fed the diet with 1.5 g AMP kg?1, followed by fish fed diets with 1.5 g UMP and mixed‐NT kg?1, and lowest in the control group. These results suggest that growth and immune responses were enhanced in grouper fed diet with 1.5 g mixed‐NT kg?1 diet. Diet with 1.5 g kg?1 of AMP seems to be more beneficial on the immune responses in fish than other nucleotides. 相似文献
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ABSTRACT : The aim of the present study was to investigate carbohydrate utilization by the grouper Epinephelus malabaricus reared at 23°C. Two isoenergetic semipurified diets were prepared with two carbohydrate sources (glucose and starch). Each diet was fed to triplicate groups of grouper in a recirculating rearing system for 8 weeks. Water temperature was held constant by a thermal controller at 23 ± 1°C. Weight gain, feed efficiency (FE) and protein efficiency ratio (PER) of fish fed the starch diet were significantly higher ( P < 0.05) than those of fish fed the glucose diet. Body lipid content of the starch-fed group of fish was higher than that of glucose-fed group of fish. Hepatic hexokinase and glucose-6-phosphate dehydrogenase activities were higher in fish fed the starch diet than fish fed the glucose diet. Fish fed the glucose diet had higher hepatic glucose-6-phosphatase activity than fish fed the starch diet. These results suggest that starch is better utilized by grouper than glucose when the water temperature is 23°C. 相似文献
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A growth trial was conducted to investigate the effects of fish oil and corn oil on the growth and non‐specific immune responses of juvenile grouper, Epinephelus malabaricus. Five semi‐purified diets were supplemented with 40 g kg−1 of either fish oil (F4), corn oil (C4) or blend of fish oil with corn oil at ratio of 3 : 1 (F3C1); 1 : 1 (F2C2) and 1 : 3 (F1C3). Each diet was fed to triplicate groups of grouper (mean initial weight: 10.26 ± 0.14 g) in a recirculating rearing system for 8 weeks. Weight gain and feed efficiency of fish fed the F4, F3C1 and F2C2 diets were the highest (P < 0.05), followed by fish fed the F1C3 diet, and the lowest in fish fed the C4 diet. Fish fed the C4 diet had a lower survival rate than fish on other dietary treatments. Fatty acid composition of liver and muscle in fish generally reflected the composition of the diet. Leukocyte superoxide anion (O−2) production ratio was the highest in fish fed the F3C1 and F2C2 diets, followed by fish fed the F4 and F1C3 diets, and the lowest in fish fed the C4 diet. Fish fed the F3C1 and F2C2 diets had higher plasma lysozyme activities than fish fed the F4 and C4 diets. Plasma alternative complement activity was higher in fish fed the F3C1, F2C2 and F1C3 diets than fish fed the F4 and C4 diets. These results suggest that grouper fed diets with 3 : 1 or 1 : 1 of fish oil to corn oil ratio had similar growth to the fish fed diet with fish oil. Blend of fish oil with corn oil in diet significantly enhanced non‐specific immune responses of grouper when the fed diet contained fish oil as the only lipid source. 相似文献
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