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为了创立在用户庭院就能应用的玻璃化保存性别控制胚胎的冷冻剂稀释处理方法，采用细管内3种不同液体层构成（A、B、C）法，对新鲜分割胚一步法稀释后的存活能力进行了研究；其次用特定细管最小容量冷却法与上述3种不同液体层构成中效果较好的B法对玻璃化冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚进行一步法稀释后的存活能力予以对比。结果，3种不同液体层构成间胚胎的存活能力无显著差异，B法较高；应用特定细管最小容量冷却法在冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚一步法稀释后的存活能力均显著高于B法。结果表明，特定细管最小容量冷却法是更有效的保存方法。 相似文献
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In the coastal zone of the Sea of Okhotsk off Hokkaido, Japan, the giant ezo scallop ( Patinopecten yessoensis ) is widely cultivated on the sea bed, making its fishery a major industry. However, the area experiences intense cyclones, including typhoons, about once a decade and the scallop resources appear to have received severe damage from the storms. Such an event occurred on 29 October 1988. The storm caused 12-m-high wind waves, which appeared to be fully developed sea waves. The water above the sea bed repeatedly moved back and forth at speeds of 1∼2 m s-1 causing damage to the scallops due to collisions and rubbing with gravel and other scallops. From a comparison of wind data with the annual catch of scallops, intense cyclones seem to play an important role on the year-to-year fluctuations in the scallop fishery. 相似文献
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Tomoaki ONODA Ryuta YAMAMOTO Kyohei SAWAMURA Harutaka MURASE Yasuo NAMBO Yoshinobu INOUE Akira MATSUI Takeshi MIYAKE Nobuhiro HIRAI 《Journal of Equine Science》2013,24(4):63-69
Percentile growth curves are often used as a clinical indicator to evaluate variations of
children’s growth status. In this study, we propose empirical percentile growth curves
using Z-scores adapted for Japanese Thoroughbred horses, with considerations of the
seasonal compensatory growth that is a typical characteristic of seasonal breeding
animals. We previously developed new growth curve equations for Japanese Thoroughbreds
adjusting for compensatory growth. Individual horses and residual effects were included as
random effects in the growth curve equation model and their variance components were
estimated. Based on the Z-scores of the estimated variance components, empirical
percentile growth curves were constructed. A total of 5,594 and 5,680 body weight and age
measurements of male and female Thoroughbreds, respectively, and 3,770 withers height and
age measurements were used in the analyses. The developed empirical percentile growth
curves using Z-scores are computationally feasible and useful for monitoring individual
growth parameters of body weight and withers height of young Thoroughbred horses,
especially during compensatory growth periods. 相似文献
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Takahisa YAMADA Seiki SASAKI Shin SUKEGAWA Takeshi MIYAKE Tatsuo FUJITA Hiroyuki KOSE Mitsuo MORITA Youichi TAKAHAGI Hiroshi MURAKAMI Fumiki MORIMATSU Yoshiyuki SASAKI 《Animal Science Journal》2009,80(4):486-489
Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation , sphingolipid G-protein-coupled receptor , 1 ( EDG1 ) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A > G in the 5' untranslated region (UTR) and c.*446G > A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G > A and g.1471620G > T , in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G > A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G > T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G > T SNP might have an impact on EDG1 expression and also marbling. 相似文献
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Masato MIYAKE Shinichiro HAYASHI Yoshikazu TAKETA Shunsuke IWASAKI Kouichi WATANABE Shyuich OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2010,81(2):223-229
Myostatin (MSTN) is a negative regulator during muscle differentiation, whereas insulin‐like growth factors (IGFs) are essential for muscle development. MSTN and IGFs act oppositely during myogenesis, but there is little information on the mutual relationship of MSTN and IGFs. The present study was conducted to examine whether MSTN affects IGF expression during early myogenesis in cattle. IGF‐1 mRNA was similarly expressed in M. longissimus thoracis of double‐muscled (DM) and normal (NM) Japanese shorthorn cattle. IGF‐2 mRNA expression was consistently higher in the normal and regenerating muscle of DM cattle than those of NM cattle. When myoblasts were isolated from regenerating M. longissimus thoracis, IGF‐2 mRNA expression showed a significant increase in differentiating DM derived myoblasts (DM‐myoblasts) as compared with differentiating NM derived myoblasts (NM‐myoblasts). An addition of recombinant mouse myostatin (rMSTN) to myoblast cultures attenuated IGF‐2 mRNA expression and decreased myotube formation, but did not effect IGF‐1 mRNA expression. An activin‐like kinase (ALK) inhibitor, SB431542, mediates MSTN action, suppressed the translocation of Smad2/3 into the nucleus in DM‐myoblasts, and restored the attenuated IGF‐2 mRNA expression and the decreased myotube formation induced by rMSTN in myoblast cultures. The findings indicate that MSTN may negatively regulate myoblast differentiation by suppressing IGF‐2 expression via ALK‐Smad signaling. 相似文献
7.
Yoshikazu TAKETA Yasuhiro NAGAI Hideki OGASAWARA Shinichiro HAYASHI Masato MIYAKE Sachi TANAKA Kouichi WATANABE Shyuichi OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2008,79(3):382-390
Myostatin (MSTN), known as growth and differentiation factor 8 (GDF-8), is a member of the transforming growth factor β (TGF-β) superfamily that negatively regulates skeletal muscle mass. Myostatin binds with high affinity to the receptor serine threonine kinase activin receptor type IIB (ActRIIB). Activins that also belong to the TGF-β superfamily, stimulate follicle-stimulating hormone production in gonadotrophs and suppress growth hormone and adrenocorticotropic hormone production in somatotrophs and corticotrophs, respectively. The aim of the present paper was therefore to clarify the endocrine action of MSTN in adenohypophysis. The present study details the expression and cellular localization of MSTN and ActRIIB in porcine anterior pituitary gland. The mRNA of MSTN and ActRIIB was consistently expressed in RT-PCR. Immunohistochemistry of MSTN and specific hormones showed that MSTN localized in thyrotrophs and gonadotrophs, in which most of the MSTN immunoreactive cells were identified as thyrotrophs. The immunostaining of ActRIIB was restricted to corticotrophs. These results indicate that MSTN was mainly produced in thyrotrophs and its receptor, ActRIIB, was restrictively contained in corticotrophs. Interestingly, thyrotrophs immunoreactive for MSTN were frequently close to corticotrophs immunoreactive for ActRIIB. The present study suggests that MSTN from thyrotrophs may regulate corticotroph function as a paracrine mediator among the porcine anterior pituitary cells. 相似文献
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YASUYUKI TSUKAMASA YASUYOSHI MIYAKE MASASHI ANDO YASUO MAKINODAN 《Fisheries Science》2002,68(4):929-933
ABSTRACT: Transglutaminase seems to be related to the setting phenomenon of fish meat paste that occurs at temperatures below 40°C. In many reports on the relationship between transglutaminase and setting phenomenon, the enzyme activity has been measured at 25°C. However, it is known that the setting phenomenon is complicated and the effect of calcium and the 3-D structure of myofibrils, which are sensitive to temperature, play important roles in the reaction. In the present study, total activities of transglutaminase of threadfin bream, white croaker, red sea bream and carp meats were measured at various temperatures. Total transglutaminase activities at 25°C of tested fish meats are arranged in order as follows; white croaker, red sea bream, carp, threadfin bream. In contrast, optimal temperature of carp meat is 30°C, red sea bream 40°C, threadfin bream 50°C and white croaker 50–55°C. In carp meat, the enzyme activity at optimal temperature (30°C) became 8.5-fold higher than at 25°C. The data of the total activity at various temperatures are useful in order to comprehend the details of the reaction. 相似文献
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Ryoka MIYAKE Takakaki ITO Hiroshi KAMATA Rui KANO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1090
We isolated ascomycetous yeasts including Candida species, that originally belonged to the genus Candida, from bulk milk in the Aichi area of Japan, and determined the minimum inhibitory concentrations (MICs) of antifungal drugs on these isolates by conducting E-tests. We isolated 7 human pathogenic species (14 isolates) from 14 bulk milk samples: 5 Candida species of yeasts, and 2 Candida-related species. Two isolates of C. albicans and C. inconspicua were resistant to fluconazole (MIC >32 mg/l). One isolate of C. krusei was resistant to both azoles (fluconazole: >256 mg/ml and itraconazole: 4 mg/l). One isolate of C. catenulata might be resistant to amphotericin B (>32 mg/l). 相似文献