Kalmias and rhododendrons     

Abbott CC 《Science (New York, N.Y.)》1883,2(28):201-202

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Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)          下载免费PDF全文

DSR Angrimani  KK Nagai  BR Rui  LC Bicudo  JDA Losano  MM Brito  MCP Francischini  M Nichi 《Reproduction in domestic animals》2018,53(1):163-170

Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer‐assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3′3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.  相似文献   

A fast,low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species          下载免费PDF全文

BR Rui  DSR Angrimani  LC Bicudo  JDA Losano  M Nichi  RJG Pereira 《Reproduction in domestic animals》2018,53(1):171-175

Sperm DNA fragmentation is a condition that interferes directly in the reproductive efficiency. Currently, there are several methods for assessing the sperm DNA integrity, such as Alkaline Comet, TUNEL and Sperm Chromatin Structure Assay. However, many of these techniques are laborious and require high‐precision equipment. Thus, the development of new techniques can optimize the evaluation of sperm DNA damage. Therefore, the aim of this study was to standardize the toluidine blue (TB) stain technique for the analysis of DNA fragmentation of dog, cat, bull, stallion and ram spermatozoa. For this purpose, we used six animals of each specie (n = 30), in reproductive age. Sperm was collected by different methods according to the particularities of each species, and such samples were divided into two aliquots: a sperm sample was kept at 5°C (considered as intact sperm DNA), and the remaining samples were submitted to the induction of DNA fragmentation by exposure to ultraviolet light for 4 hr. Samples were then mixed with the intact sample to obtain known and progressive proportions of sperm with fragmented DNA (0%, 25%, 50%, 75% and 100%). Semen smears were performed and subjected to staining with TB. Blue‐stained spermatozoa were considered to have DNA fragmentation. We observed high linear regression coefficients between the expected proportion of damaged DNA and the results of TB for dog, cat, ram, bull and stallion samples. In conclusion, TB stain was considered a fast and effective technique for the study of spermatozoa DNA in several species.  相似文献   

Effect of Pimobendan in Dogs with Preclinical Myxomatous Mitral Valve Disease and Cardiomegaly: The EPIC Study—A Randomized Clinical Trial          下载免费PDF全文

A. Boswood  J. Häggström  S.G. Gordon  G. Wess  R.L. Stepien  M.A. Oyama  B.W. Keene  J. Bonagura  K.A. MacDonald  M. Patteson  S. Smith  P.R. Fox  K. Sanderson  R. Woolley  V. Szatmári  P. Menaut  W.M. Church  M. L. O'Sullivan  J.‐P. Jaudon  J.‐G. Kresken  J. Rush  K.A. Barrett  S.L. Rosenthal  A.B. Saunders  I. Ljungvall  M. Deinert  E. Bomassi  A.H. Estrada  M.J. Fernandez Del Palacio  N.S. Moise  J.A. Abbott  Y. Fujii  A. Spier  M.W. Luethy  R.A. Santilli  M. Uechi  A. Tidholm  P. Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(6):1765-1779

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Characterization of multidrug resistant Salmonella recovered from diseased animals     

Zhao S  McDermott PF  White DG  Qaiyumi S  Friedman SL  Abbott JW  Glenn A  Ayers SL  Post KW  Fales WH  Wilson RB  Reggiardo C  Walker RD 《Veterinary microbiology》2007,123(1-3):122-132

Three hundred and eighty Salmonella isolates recovered from animal diagnostic samples obtained from four state veterinary diagnostic laboratories (AZ, NC, MO, and TN) between 2002 and 2003 were tested for antimicrobial susceptibilities and further characterized for bla(CMY) beta-lactamase genes, class 1 integrons and genetic relatedness using PFGE. Forty-seven serovars were identified, the most common being S. Typhimurium (26%), S. Heidelberg (9%), S, Dublin (8%), S. Newport (8%), S. Derby (7%), and S. Choleraesuis (7%). Three hundred and thirteen (82%) isolates were resistant to at least one antimicrobial, and 265 (70%) to three or more antimicrobials. Resistance was most often observed to tetracycline (78%), followed by streptomycin (73%), sulfamethoxazole (68%), and ampicillin (54%), and to a lesser extent chloramphenicol (37%), kanamycin (37%), amoxicillin-clavulanic acid (20%), and ceftiofur (17%). With regards to animal of origin, swine Salmonella isolates displayed the highest rate of resistance, being resistant to at least one antimicrobial (92%), followed by those recovered from turkey (91%), cattle (77%), chicken (68%), and equine (20%). Serovars commonly showing multidrug resistance (MDR) to > or =9 antimicrobials were S. Uganda (100%), S. Agona (79%), and S. Newport (62%), compared to S. Heidelberg (11%) and S. Typhimurium (7%). Class-1 integrons were detected in 43% of all isolates, and were found to contain aadA, aadB, dhfr, cmlA and sat1 gene cassettes alone or in various combinations. All ceftiofur resistant isolates (n=66) carried the bla(CMY) beta-lactamase gene. A total of 230 PFGE patterns were generated among the 380 isolates tested using XbaI, indicating extensive genetic diversity across recovered Salmonella serovars, however, several MDR clones were repeatedly recovered from different diseased animals.  相似文献   

Retrospective analysis of immunosuppressive and anti-thrombotic protocols in nonassociative immune mediated hemolytic anemia in dogs     

Jennifer Weng  Nyssa A. Levy  Haley Y. Abbott  Jose A. Mix  Robert W. Wills  Andrew J. Mackin  John M. Thomason  Harry Cridge 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):528-536

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Determination of oil,emulsifier and polymer or salt content of spray adjuvants     

Henry A. Abbott  Pamela L. Bronner 《Pest management science》1986,17(2):103-108

A method for the separation of the oil, emulsifier and polymer or salt fractions of adjuvants is described. The adjuvant is dissolved in 90% aqueous ethanol and filtered to remove polymers or salt. Light petroleum (distillation range 30–40°C) is added to the solution in a separating funnel. The light petroleum fraction is subsequently extracted with successive aliquots of 80, 70, 60 and 50% aqueous ethanol. The whole of the ethanol and light petroleum fractions are evaporated to dryness to yield the emulsifier and oil fractions respectively. Emulsifier, oil and polymer or salt are determined gravimetrically. The method successfully detected the presence of polymer or salt in adjuvants, but could not distinguish between them when both were present in the same product. The adjuvants could be classified into four broad classes; those containing > 70% emulsifier (wetter-spreaders), those containing > 75% oil (anti-evaporants), those containing a high proportion of polymer (film-forming substances), and those containing comparable amounts of all three components (multi-purpose adjuvants).  相似文献   

Some aspects of crop variation in ‘Golden Delicious’ apple trees     

J.M. Silva  M.E. Holgate  D.L. Abbott 《Scientia Horticulturae》1980,13(1):27-32

Fruit-set of ‘Golden Delicious’ apple trees was found to be largely independent of seasonal differences and much more closely related to the initial number of blossom clusters, when compared with many other apple cultivars under English conditions. In one young orchard, yield became increasingly a function of trunk girth during the first 3 cropping-years, but the trees then abruptly developed a biennial rhythm. Thereafter, blossom production was negatively correlated with the number of clusters in the previous year rather than with the number of fruits.Applications of daminozide or GA₃ appeared to increase or decrease, respectively, the amount of return bloom without altering the negative correlation with the number of clusters in the previous year. Chemical fruit-thinning with a mixture of carbaryl and NAA also appeared to be effective in increasing return bloom.  相似文献   

The effect of dietary protein on the pathogenesis of acute ovine haemonchosis     

E. M. Abbott  J. J. Parkins  P. H. Holmes 《Veterinary parasitology》1986,20(4):275-289

Two experiments were conducted to examine the ifluence of dietary proteins on parasite establishment and pathogenesis in Finn Dorset/Dorset Horn lambs infected with Haemonchus contortus. The lambs were introduced to high (169 g) or low (88 g crude protein (CP) kg⁻¹ dry matter (DM)) protein diets at 3 months of age and infected 1 month later with 350 larvae kg⁻¹ body weight (BW). Blood and faecal smaples were collected for analysis and body weights recorded weekly. In the first experiments some of the infected lambs were killed 6 weeks after infection and the remainder 5 weeks later. In the second experimental all the infected lambs were killed 4 weeks after infection.

The results showed that lambs on a low protein diet were less able to withstand the pathogenic effects of infection with 350 H. contortus larvae kg⁻¹ BW than lambs given the higher protein diet. Thus mortality was greater in the low protein group and adverse clinical signs, such as inappetance, weight loss and oedema were osbserved more frequently. This group also had a more severe anaemia, hypoproteinaemia and hypoalbuminnaemia than the high protein group. In contrast, faecal egg counts, total daily faecal egg output and worm burdens were similar in all groups of infected lambs, indicating that the diets did not influence parasite establishment.  相似文献   

Incidentally detected heart murmurs in dogs and cats: executive summary 2015          下载免费PDF全文

E. Côté  N. J. Edwards  S. J. Ettinger  V. L. Fuentes  K. A. MacDonald  B. A. Scansen  D. D. Sisson  J. A. Abbott 《The Journal of small animal practice》2015,56(10):593-594

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