The disposition of carbenicillin in blood,other body fluids and tissues of buffalo calves (Bubalus bubalis)     

K. S. Brar  M. S. Bal 《Veterinary research communications》1993,17(2):119-122

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Maternal immunity to infectious bovine rhinotracheitis and bovine viral diarrhea viruses: duration and effect on vaccination in young calves.     

J S Brar  D W Johnson  C C Muscoplat  R E Shope  J C Meiske 《American journal of veterinary research》1978,39(2):241-244

The immune response to modified live-virus bovine viral diarrhea (BVD) vaccine and infectious bovine rhinotracheitis (IBR) vaccine was examined in calves that had received passive maternal antibodies to these viruses. Blood serum samples from vaccinated and control (nonvaccinated) calves were examined for more than 1 year to determine the rate of decline of passive anti-BVD and anti-IBR antibodies and the effect that vaccination had on these antibody titers. The control calves lost their antibodies to BVD and IBR viruses at the rate of one half their remaining antibody titer every 21 days. Calves serologically responded to BVD vaccine at a time when maternal antibody titers remained between 1:96 and 1:20. However, animals did not seroconvert to the IBR vaccine until maternal antibodies had decreased and become undetectable. Evidence is presented to show that although passive immunity will inhibit IBR vaccination, priming for a secondary response will occur so that on subsequent vaccination, at a time when maternal antibodies have disappeared, the animals will respond anamnestically to IBR vaccination.  相似文献   

Outbreaks of proliferative haemorrhagic enteropathy on two pig farms   总被引：2，自引：0，他引：2  

PK HOLYOAKE  RS CUTLER 《Australian veterinary journal》1995,72(7):253-256

SUMMARY Clinical signs of proliferative haemorrhagic enteropathy (PHE) including anaemia, dysentery and sudden death were observed in finisher pigs and young breeding stock on 2 farms. On farm A, PHE occurred 12 months after repopulation of the farm. Other outbreaks of PHE occurred after the withdrawal of therapeutic concentrations of in-feed antibacterial agents (farm A), or after monensin sodium (100 g/t) replaced olaquindox (100 g/t) in feed (farm B). The outbreaks, the possible sources of contamination and the role of antibacterial feed additives in the treatment and control of PHE are described.  相似文献   

Comparative Expression Analysis of Gametogenesis‐Associated Genes in Foetal and Adult Bubaline (Bubalus bubalis) Ovaries and Testes          下载免费PDF全文

SM Shah  N Saini  S Ashraf  M Zandi  MK Singh  RS Manik  SK Singla  P Palta  MS Chauhan 《Reproduction in domestic animals》2015,50(3):365-377

This study was conducted to identify and analyse the expression of gametogenesis‐associated genes and proteins in foetal and adult buffalo gonads of both the sexes. Relative quantification of the genes was determined by qPCR and Western blotting. Immunohistochemistry was also performed for various gametogenesis‐associated proteins in foetal and adult gonads of both the sexes. We observed significantly (p < 0.05) increased expression of primordial germ cell‐specific, meiotic as well as genes associated with oocyte maturation and development in foetal ovaries as compared to the adult ones. However, significantly (p < 0.05) increased expression of proteins associated with oocyte maturation like GDF9 and ZP4 was found in adult ovaries, indicating temporal regulation of mRNA translation during oogenesis. Meiotic genes showed significantly (p < 0.05) increased expression in adult testes as compared to foetal testes and ovaries, indicating onset of meiosis at a later stage in spermatogenesis. In general, the expression of primordial germ cell‐associated as well as meiotic genes was higher in adult testes, indicating the increased biological activity in the organ. Immunohistochemistry revealed localized expression of gametogenesis‐associated proteins in ovarian follicles and seminiferous tubules of testes, while the surrounding somatic tissues were devoid of these proteins. The study gives an understanding of the sequential and temporal events of gene expression as well as mRNA translation during male and female gametogenesis. It could also be concluded that follicles and seminiferous tubules are the functional units of the female and male gonads, respectively, and their function could be enhanced by appropriate chemical and genetic intervention of the somatic tissue immediately surrounding them. This assumes importance in the context that buffalo attains sexual maturity at an older age of 2–3 years and have smaller ovaries with lesser number of primordial follicles in comparison with cattle, which is suggested to be the main reason of their poor breeding performance.  相似文献   

Production of Wild Buffalo (Bubalus arnee) Embryos by Interspecies Somatic Cell Nuclear Transfer Using Domestic Buffalo (Bubalus bubalis) Oocytes     

D Priya  NL Selokar  AK Raja  M Saini  AA Sahare  N Nala  P Palta  MS Chauhan  RS Manik  SK Singla 《Reproduction in domestic animals》2014,49(2):343-351

The objective of this study was to explore the possibility of producing wild buffalo embryos by interspecies somatic cell nuclear transfer (iSCNT) through handmade cloning using wild buffalo somatic cells and domestic buffalo (Bubalus bubalis) oocytes. Somatic cells derived from the ear skin of wild buffalo were found to express vimentin but not keratin and cytokeratin‐18, indicating that they were of fibroblast origin. The population doubling time of skin fibroblasts from wild buffalo was significantly (p < 0.05) higher, and the cell proliferation rate was significantly (p < 0.05) lower compared with that of skin fibroblasts from domestic buffalo. Neither the cleavage (92.6 ± 2.0% vs 92.8 ± 2.0%) nor the blastocyst rate (42.4 ± 2.4% vs 38.7 ± 2.8%) was significantly different between the intraspecies cloned embryos produced using skin fibroblasts from domestic buffalo and interspecies cloned embryos produced using skin fibroblasts from wild buffalo. However, the total cell number (TCN) was significantly (p < 0.05) lower (192.0 ± 25.6 vs 345.7 ± 42.2), and the apoptotic index was significantly (p < 0.05) higher (15.1 ± 3.1 vs 8.0 ± 1.4) for interspecies than that for intraspecies cloned embryos. Following vitrification in open‐pulled straws (OPS) and warming, although the cryosurvival rate of both types of cloned embryos, as indicated by their re‐expansion rate, was not significantly different (34.8 ± 1.5% vs 47.8 ± 7.8), the apoptotic index was significantly (p < 0.05) higher for vitrified–warmed interspecies than that for corresponding intraspecies cloned embryos (48.9 ± 7.2 vs 23.9 ± 2.8). The global level of H3K18ac was significantly (p < 0.05) lower in interspecies cloned embryos than that in intraspecies cloned embryos. The expression level of HDAC1, DNMT3a and CASPASE3 was significantly (p < 0.05) higher, that of P53 was significantly (p < 0.05) lower in interspecies than in intraspecies embryos, whereas that of DNMT1 was similar between the two types of embryos. In conclusion, these results demonstrate that wild buffalo embryos can be produced by iSCNT.  相似文献   

Energy input–output modeling and sensitivity analysis for on-farm mechanically paddy straw bales formation     

Sharma  Ankit  Brar  Amandeep Singh 《Paddy and Water Environment》2022,20(2):255-264

Paddy and Water Environment - The paddy straw was collected in the form of bales with three mechanical process CI (stubble shaver + rectangular baler), CII (stubble...  相似文献   

The efficacy of insecticides against the screw-worm fly (Chrysomya bezziana)     

JP SPRADBERY  RS TOZER†  AA POUND 《Australian veterinary journal》1991,68(10):338-342

A number of insecticides used for ectoparasite control in the livestock industry were screened for their efficacy against larvae of the screw-worm fly, Chrysomya bezziana, using in vivo and laboratory tests. Proprietary screw-worm fly treatments (after exposure to outdoor conditions for up to 10 days) were also tested against eggs and adults of C bezziana. Three of these were also evaluated on naturally acquired screw-worm infestations. Residual protection was generally of short duration. Among the organophosphorus compounds, the most effective formulations contained relatively high concentrations (3 to 4% al) of coumaphos, 2.5% fenchlorphos or low concentrations (0.05 to 0.5% al) of diazinon, chlorfenvinphos and fenthion methyl. Two chlorinated hydrocarbon insecticides containing 3% lindane and 5% dieldrin were very effective but are now prohibited for use in Australia. Preparations had serious deficiencies when used under field conditions, especially for treating large, deepseated myiases for which systemic insecticides are recommended. A comparison of methods demonstrated that a laboratory test could supersede live animal experimentation, at least for the initial screening of potential insecticides.  相似文献   

Inheritance of a plant type mutant and its utilization in chickpea     

J. S. Sandhu  H. S. Brar  M. M. Verma 《Euphytica》1990,48(2):111-112

Summary A macro-mutant, E 100Y(M) in chickpea (Cicer arietinum L.) was found to affect several plant and seed characters. For plant type monogenic inheritance was observed. A single pair of recessive genes pt/pt was ascribed to this mutant. The mutant locus seemed to be exerting pleiotropic action. The utilization of this mutant for chickpea improvement has been discussed.  相似文献   

Drip irrigation and nitrogen fertilization alter phenological development and yield of spring maize (Zea mays L.) under semi-arid conditions     

Harjeet Singh Brar  Krishan Kumar Vashist 《Journal of plant nutrition》2020,43(12):1757-1767

Abstract

Maize response to deficit water and nitrogen for assessing phenological development and yield was studied under semi-arid conditions. Experiment consisting three drip irrigation levels, replenish 60 (DI₆₀), 80 (DI₈₀) and 100 percent (DI₁₀₀) of cumulative pan evaporation, and four nitrogen doses 50 (RN₅₀), 75 (RN₇₅), 100 (RN₁₀₀) and 125 (RN₁₂₅) per cent of recommended nitrogen. A Furrow irrigated treatment was kept as control/check. Significant earliness in visibility of collar of 8^th leaf, tasseling, silking and significant delay in dough stage and physiological maturity was recorded under well water treatment DI₁₀₀ as compared to DI₆₀. Days to collar of 8^th leaf, tasseling and silking had significant negative correlation, and duration of yield formation phase and days to physiological maturity had significant positive correlation with grain yield. Higher DM production, longer yield formation phase and late physiological maturity led to significantly higher grain yield under DI₁₀₀. In case of nitrogen levels, phonological characteristics like collar of 8^th leaf, tasseling and silking were significantly delayed, and dough stage and physiological maturity were advanced under nitrogen deficit treatment RN₅₀ as compared to RN₁₀₀ and RN₁₂₅. Significantly higher dry matter production and longer yield formation phase observed led to significant higher SCY under RN₁₀₀ and RN₁₂₅ as compared to RN₅₀. Yield formation phase was significantly longer under drip irrigated crop as compared to control during 2^nd year of study. Crop phenological development significantly affected by drip irrigation regimes and nitrogen levels, and there was significant correlation between phenological stages development and grain yield.  相似文献   

Genetic Diversity Based on Allozyme Alleles of Chinese Cultivated Rice     

TANG Sheng-xiang  WEI Xing-hua  JIANG Yun-zhu  D S Brar  G S Khush 《中国农业科学(英文版)》2007,6(6)

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