全文获取类型
收费全文 | 470篇 |
免费 | 9篇 |
专业分类
林业 | 51篇 |
农学 | 22篇 |
基础科学 | 6篇 |
126篇 | |
综合类 | 46篇 |
农作物 | 12篇 |
水产渔业 | 31篇 |
畜牧兽医 | 145篇 |
园艺 | 12篇 |
植物保护 | 28篇 |
出版年
2023年 | 3篇 |
2022年 | 1篇 |
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 12篇 |
2017年 | 4篇 |
2016年 | 8篇 |
2015年 | 5篇 |
2014年 | 16篇 |
2013年 | 20篇 |
2012年 | 38篇 |
2011年 | 41篇 |
2010年 | 20篇 |
2009年 | 21篇 |
2008年 | 46篇 |
2007年 | 37篇 |
2006年 | 32篇 |
2005年 | 25篇 |
2004年 | 19篇 |
2003年 | 21篇 |
2002年 | 28篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1992年 | 4篇 |
1990年 | 2篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1967年 | 4篇 |
1956年 | 1篇 |
1940年 | 2篇 |
1936年 | 2篇 |
1934年 | 3篇 |
1933年 | 2篇 |
排序方式: 共有479条查询结果,搜索用时 15 毫秒
1.
Ohne Zusammenfassung 相似文献
2.
3.
Pille F Martens A Schouls LM Dewulf J Decostere A Vogelaers D Gasthuys F 《Veterinary journal (London, England : 1997)》2007,173(1):73-78
The objectives of the present study were to evaluate the accuracy of broad range 16S rRNA gene PCR compared to bacterial culture for the detection of synovial infection in horses. The study included 57 synovial fluid samples from horses with presumed synovial infection and a control group consisting of 31 synovial fluid samples originating from clinically normal horses and horses with aseptic synovial inflammation. All samples were analysed by 16S PCR with reverse line blot (RLB) hybridisation. Synovial fluid samples were cultured using conventional agar plate methods (APM) and/or blood culture medium (BCM). The results of the study showed a superior detection rate (89.5%) for 16S PCR with RLB. Bacterial culture had lower sensitivity, but highly acceptable detection rates (77.6%) were observed using BCM. APM had very low sensitivity (37.8%) and infection was never detected by plate isolation without positive incubation in BCM. The highest sensitivity (91.8%) for the detection of synovial infection was achieved when the results of incubation in BCM and 16S PCR were combined. For all the tests, the specificity was higher than 90%. 相似文献
4.
Journal of Plant Diseases and Protection - The host response of 17 cover crop cultivars to infection by single-species populations of Pratylenchus hippeastri, Pratylenchus vulnus and Pratylenchus... 相似文献
5.
Karel Lavrijsen Jos Van Houdt Dirk Van Dyck Willem Meuldermans Jos Heykants 《Pest management science》1990,29(1):47-56
Bobwhite quails were treated with imazalil for 8 weeks. The fungicide was given admixed in the food at 0, 100, 300, 500 and 1000 mg kg?1. Even at the highest dose tested, imazalil did not affect the liver weight or the hepatic microsomal protein content. In treated quails, no significant induction of cytochrome P-450 or NADPH-cytochrome c-reductase activity was observed. Furthermore, imazalil did not induce or inhibit 7-ethoxyresorufin or 7-ethoxycoumarin O-deethylase in quail microsomes. Only a slight but significant increase by 35% and 49% in aniline hydroxylase activity was measured for the 500- and 1000-mg kg?1 dose levels, respectively. After a drug-free period of one week, aniline hydroxylase activity returned to control values, indicating that the effect was fully reversible. It is concluded that imazalil does not induce or inhibit drug-metabolizing enzymes in the quail, even at doses which exceed by far the maximum levels currently used to dress seed under field conditions (100 mg kg?1). 相似文献
6.
Leontine T. Colon Dirk J. Budding L. C. Paul Keizer Martin M. J. Pieters 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(4):441-456
Four components of partial resistance toPhytophthora infestans were measured after inoculation in the greenhouse and in the field ofSolanum arnezii x hondelmannii, S. berthaultii, S. circaeifolium, S. leptophyes, S. microdontum, S. sparsipilum, S. sucrense andS. vernei, and four hybrid progenies ofS. microdontum withS. tuberosum. The four components were infection efficiency, lesion growth rate, generation time and sporulation capacity. The results were compared with resistance ratings derived from field experiments, and with observations made on the potato cultivars Bintje, Bildstar, Libertas and Pimpernel. Genetic variation for all components was found, while the relative importance of the components of partial resistance appeared to vary between the species. InS. microdontum, generation time, infection efficiency and lesion growth rate, and inS. tuberosum infection efficiency, lesion growth rate and sporulation capacity appeared positively associated, but in other species no such association was found. A strong hypersensitive reaction, the expression of which appeared to depend on environmental conditions, was found inS. microdontum. ForS. berthaultii, infection efficiency appeared to be the main resistance component.Abbreviations ADPC
area under the disease progress curve
- IE
infection efficiency
- LGR
lesion growth rate
- GT
generation time
- SC
sporulation capacity 相似文献
7.
Classification of Dutch and German avian reoviruses by sequencing the sigma C protein 总被引:1,自引:0,他引:1
Kant A Balk F Born L van Roozelaar D Heijmans J Gielkens A ter Huurne A 《Veterinary research》2003,34(2):203-212
We have amplified, cloned and sequenced (part of) the open reading frame of the S1 segment encoding the sigma C protein of avian reoviruses isolated from chickens with different disease conditions in Germany and The Netherlands during 1980 up to 2000. These avian reoviruses were analysed phylogenetically and compared with sequences of avian reoviruses in the Genbank database. The avian reoviruses could be grouped in 5 different genotyping clusters and this classification was identical when the sequences were compared of the 5' end, the 3' end or the whole open reading frame of the sigma C protein. Therefore sequencing of either part of the gene encoding the sigma C protein seems to be reliable for classification. We were unable to identify a correlation between sigma C sequences of the avian reoviruses and the disease condition they were isolated from. The sequences found in The Netherlands and in Germany are, like those in Taiwan, more dispersed than the known avian reovirus sigma C sequences in the USA and Australia. We did not establish temporal or geographic differences in the avian reoviruses studied. 相似文献
8.
Factors affecting the efficacy of an attracticide strategy for the control of the codling moth Cydia pomonella L (Lepidoptera: Tortricidae) were investigated using laboratory and field experiments. The sex-pheromone-based insect-control strategy utilises 100-microliters droplets of a sticky, paste-like formulation containing 1 mg g-1 (E,E)-8,10-dodecadien-1-ol (codlemone) as an attractant for male moths and 40 mg g-1 cyfluthrin, a contact insecticide, applied to branches in the upper parts of the tree crown. The longevity of the treatment under field conditions was assessed in the laboratory by biological testing of variously aged samples of the attracticide formulation which had been applied to the bark of apple trees growing in commercial orchards. Electroantennogram responses of male moth antennae were used to compare codlemone release from the attracticide after different lengths of environmental exposure. Changes in insecticidal efficacy of the same samples were assessed with reference to the speed of knockdown (KT50) and the mortalities after 48 h among populations of male moths confined in cages containing samples of fresh and field-aged formulations. Gradual declines in both the amount of pheromone released and insecticidal activity were observed over the 10-week period of the experiments. Various factors associated with the behaviour of codling moths in the field which might influence the attracticide strategy were also investigated. Although the vertical position of attracticide sources within apple trees had a strong influence on their attractiveness, their horizontal position had none. Results of field trials showed that efficacy of the attracticide depends on the population density of the pest. Under the conditions of the current study a density of three or more sources per tree (= 4500 sources per ha) was required to attain satisfactory levels of codling moth control. 相似文献
9.
Sue C. Tongue Dirk U. Pfeiffer Lindsay Heasman Hugh Simmons Stephen J. Ryder 《Livestock Science》2006,105(1-3):120-128
The National Scrapie Plan (NSP) for Great Britain includes selective breeding programmes. These aim to reduce the frequencies of the ovine prion protein (PrP) genotypes at high risk of developing clinical disease (principally those containing the VRQ allele) within the national flock, and select for the low-risk ARR-containing genotypes. The aim of this work was to use available data from embryo transfer programmes in guaranteed scrapie-free environments to investigate whether there was a difference in lamb birth weight associated with the lamb's PrP genotype, which might affect survival. The first analysis utilised data from 355 Cheviot lambs born between 2001 and 2004. The analysis was then repeated using a larger data set of 737 Cheviot, Dorset and Suffolk lambs born between 1999 and 2003. The most important determinants of mean lamb birth weight were litter size, sex and recipient ewe breed, plus lamb breed in the three breed analysis. In both analyses the effects of all these variables are consistent and of the same order of magnitude. In the first analysis there was evidence for an apparent increase in mean lamb birth weight for the ARR-containing genotypes of 0.3 kg (95% C.I. 0.1–0.5 kg). Whereas, in the second data set there was evidence for a decrease in mean lamb birth weight for the VRQ-containing genotypes of − 0.6 kg (95% C.I. − 0.8 to − 0.4 kg). This had been masked in the first analysis by the categorisation of these genotypes with the ARQ/ARQ genotype. Within these flocks, in a scrapie-free environment, neither finding provides support for concerns that selection for low risk ARR-containing genotypes would be detrimental to lamb birth weight, which is considered to be the most important determinant of lamb survival. Extension of this work into field flocks, where scrapie-status cannot be easily assured, will require the collection of sufficient data to adjust for known direct effects as well as any additional potential confounding effects. 相似文献
10.
Eduardo Segundo Dietrich E. Lesemann Germán Martín María P. Carmona Leticia Ruiz Isabel M. Cuadrado Leonardo Velasco Dirk Janssen 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):81-87
Amaranthus leaf mottle virus (AmLMV) was classified as a member of the genus Potyvirus on the basis of its particle morphology, serology, and biological properties (Casetta et al., 1986). Based on these properties, an Amaranthus viridis-infecting virus isolated in Spain, causing mottle and leaf blistering as well as reduced growth has been identified as AmLMV.
The 3′ terminal genomic region of this and a reference isolate from Italy has been sequenced and reveals a 95% nucleotide
identity between the two isolates. The sequenced part comprises the coat protein with 281 amino acids and 315 nucleotides
of the 3′ untranslated region (UTR) preceding a polyadenylated tail. Pairwise comparisons and phylogenetic analysis of the
nucleotide and deduced amino acid sequences of the CP and 3′ UTR of the cloned cDNAs with those of other potyviruses shows
that AmLMV is a distinct potyvirus closely related to Potato virus Y. 相似文献