Sensitivity,specificity, and predictive values of reagent test strip estimations of blood urea nitrogen     

Hill D  Correa MT  Stevens JB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1994,23(3):73-75

Azostix-reagent-tests(R) strips (Ames, Miles, Inc., Diagnostic Division, Elkhart, IN) were used to measure blood urea nitrogen values in blood samples from 125 dogs and cats at the North Carolina State University, College of Veterinary Medicine. Results of the tests were compared with standard serum urea nitrogen results. Sensitivity, specificity, and negative predictive values were high (86.4, 90.3, and 96.5%, respectively). Positive predictive value was low, 65.5% of the dogs and cats with elevated blood urea nitrogen values were correctly classified as abnormal The test performs well when the prevalence of abnormal values is near 50%.  相似文献   

Evaluation of a radioimmunoassay for type III procollagen peptide in the dog     

Dimski DS  Brooks CL  Johnson SE 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(2):40-44

Type III procollagen peptide (P-3-P) is a serum marker for hepatic fibrosis in humans. The utility of a commercially available radioimmunoassay for P-3-P was evaluated in the dog. The specificity of the assay was assessed by polyacrylamide gel electrophoresis (PAGE) of canine serum and purified bovine P-3-P, followed by Western immunoblotting with rabbit aniti-P-3-P serum. The sensitivity was assessed by performing the radioimmunoassay on dilutions of sera from 22 dogs. Polyacrylamide gel electrophoresis of purified bovine P-3-P and sera from two dogs suspected of having elevated P-3-P concentrations revealed no homologous bands of staining. Western immunoblotting showed marked cross-reactivity of the high antisera concentrations with several components of the serum proteins, but none corresponding to the purified P-3-P. All tested sera from dogs had minimal competitive binding with radiolabeled P-3-P in the radioimmunoassay. Dilution curves of dog sera did not parallel either the standard curve or the dilution curve of a known test human serum. There were no statistically different P-3-P concentrations in any of the groups of dogs studied. It was concluded that currently available radioimmunoassay kits for the measurement of P-3-P in the human are not applicable in the dog. Seemingly, the structure or metabolism of canine P-3-P may vary significantly from that of the bovine or human, limiting the sensitivity and specificity of this assay in the dog.  相似文献   

苹果果实日灼人工诱导技术及阈值温度研究   总被引：10，自引：3，他引：10  

张建光  刘玉芳  孙建设  施瑞德 《园艺学报》2003,30(4):446-448

 使用自制的日灼诱导设备，在无风或微风的晴天对活体果实进行田间日灼诱导，通过改变诱导室内的气温来控制和调节果面温度，从而确定了不同苹果品种果实发生日灼的阈值温度。试验证明： 自制的诱导设备运行平稳，诱导期间可保持果温基本稳定，大多数情况下，处理期间果面平均温度与实际设定温度的差异小于0．12℃ ，温度变化幅度一般小于±0．5℃ 。本试验所测定的9个苹果品种日灼阈值温度为(45．9±0．5)℃ (48．5±0．5)℃。光照对日灼发生有重要影响，即使在同样的阈值温度下，有光的处理果实发生日灼，而无光的处理则不发生日灼。  相似文献   

Expert opinion based modelling of the risk of human infection with H5N1 through the consumption of poultry meat in Germany     

Greiner M  Müller-Graf C  Hiller P  Schrader C  Gervelmeyer A  Ellerbroek L  Appel B 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(3-4):98-107

According to current scientific opinion the risk of human infection with H5N1 via preparation and consumption of poultry meat is negligible.This opinion has not yet been challenged by a formal risk assessment, due to the lack of empirical data. We have developed a scenario pathway model as a conceptual framework for a formal assessment of the H5N1 risk to humans through consumption of poultry meat and parameterise the model using information derived from expert opinions. The aim of this study was to investigate whether the notion of an overall negligible risk via the oral infection route is consistent with ad hoc data and expert opinions on the relevant parameters of the model.The model is mainly based on expert opinion. A stochastic Monte-Carlo simulation was conducted which took into consideration (amongst others) the exposure and infection of chicken (broiler and layer), turkeys, ducks and geese, the probabilities of detection prior to slaughter, virus survival and contamination during slaughter, as well as during the cutting and preparation of meat in commercial plants and in private households, respectively. The empirical consumption pattern for poultry meat in Germany was taken into account in the simulation.The results show that the risk for the individual consumer is practically zero whereas up to 23 cases per year in Germany might occur if the upper (more pessimistic) ranges of the expert opinions apply.The finding of a low but non-negligible risk to the population is discussed in relation to the epidemiological information available from recent outbreaks in South East Asia.  相似文献   

Suberitamides A–C,Aryl Alkaloids from a Pseudosuberites sp. Marine Sponge that Inhibit Cbl-b Ubiquitin Ligase Activity     

Chang-Kwon Kim  Dongdong Wang  Brice A. P. Wilson  Josep Saurí  Donna Voeller  Stanley Lipkowitz  Barry R. O’Keefe  Kirk R. Gustafson 《Marine drugs》2020,18(11)

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Recent human‐induced salinity changes in Ramsar‐listed Orielton Lagoon,south‐east Tasmania,Australia: a new approach for coastal lagoon conservation and management     

Krystyna M. Saunders  Andrew Mcminn  Donna Roberts  Dominic A. Hodgson  Henk Heijnis 《水产资源保护:海洋与淡水生态系统》2007,17(1):51-70

• 1. Many coastal lagoons in eastern Australia have changed as a result of recent human activities. However, it is often difficult to determine the extent of change and the ecological implications. A palaeoecological approach allows a quantitative assessment of how an aquatic ecosystem changes and responds to human impacts beyond what is possible with historical data or monitoring programmes.
• 2. Orielton Lagoon (south‐east Tasmania, Australia) is a Ramsar‐listed coastal wetland of international importance for conservation. This study was undertaken at the site to determine whether recent anthropogenic hydrological modifications to the lagoon had influenced its ecology, particularly salinity, and compromised its Ramsar status.
• 3. A diatom–salinity transfer function was constructed from a training data set of 96 diatom taxa from 34 sampling sites in 19 lagoons along the east coast of Tasmania.
• 4. The salinity of Orielton Lagoon has changed in response to a causeway constructed across its mouth, which has transformed the lagoon from an open marine environment to an enclosed, virtually stagnant, brackish water body.
• 5. These changes have compromised the protected coastal wetland status of Orielton Lagoon. Environmental remediation attempts have since partially restored the natural hydrology of the lagoon and it is now returning towards the state it was in prior to causeway construction.
• 6. A palaeoecological approach using diatoms was found to be successful in reconstructing recent salinity changes and investigating human impacts on Orielton Lagoon over the last 50–55 years.

Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

Student calls for education on fiscal responsibility     

Troyer D 《Journal of the American Veterinary Medical Association》2003,222(8):1073

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Suitability of SSCP-PCR analysis for molecular detection of quinolone resistance in Campylobacter jejuni     

Beckmann L  Müller M  Luber P  Schrader C  Bartelt E  Klein G 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(11-12):487-490

Foodborne infections with Campylobacter spp. are increasing, especially antibiotic resistant strains are emerging. Quinolone resistant isolates can cause failure of therapy in severe clinical infections. Molecular characterisation is needed for the detection of resistant variants of C. jejuni. Therefore 23 isolates from poultry and human medicine as well as three control strains were tested for their minimal inhibitory concentration, their Single-Strand-Conformation-Polymorphism (SSCP)-PCR pattern (a method for the detection of resistance determining point mutations), and their sequence of the quinolone resistance determining region (QRDR). Six different SSCP types could be identified: two types for quinolone resistant isolates and other types containing so called silent mutations without influence on the resistance. A genotypic monitoring of the quinolone resistance in C. jejuni can be useful for the early detection of new resistance variants. As a screening method for detection of point mutations in the QRDR the SSCP-PCR can be applied. Compared to other genotypic methods the SSCP-PCR is less time and cost consuming and needs only standard technical equipment.  相似文献   

Antigenic relationship of turkey coronavirus isolates from different geographic locations in the United States     

Lin TL  Loa CC  Wu CC  Bryan T  Hooper T  Schrader D 《Avian diseases》2002,46(2):466-472

The purpose of the present study was to examine the antigenicity of turkey coronavirus (TCV) isolates from various geographic areas with antibodies to different viruses. Seventeen isolates of TCV were recovered from intestinal samples submitted to Animal Disease Diagnostic Laboratory, Purdue University, from turkey farms located in different geographic areas. The prototype TCV Minnesota isolate (TCV-ATCC) was obtained from the American Type Culture Collection. Intestinal sections were prepared from turkey embryos infected with different TCV isolates and reacted with polyclonal or monoclonal antibodies to TCV, infectious bronchitis virus (IBV), bovine coronavirus (BCV), transmissible gastroenteritis virus (TGEV), reovirus, rotavirus, adenovirus, or enterovirus in immunofluorescent antibody staining. All 18 TCV isolates have the same antigenic reactivity pattern with the same panel of antibodies. Positive reactivity was seen with polyclonal antibodies to the TCV Indiana isolate, the TCV Virginia isolate, TCV-ATCC, and the IBV Massachusetts strain as well as monoclonal antibodies to the TCV North Carolina isolate or the membrane protein of IBV. Antibodies to BCV or TGEV were not reactive with any of the TCV isolates. Reactivity of antibodies to unrelated virus, rotavirus, reovirus, adenovirus, or enterovirus with different TCV isolates was all negative, except positive response was seen between enterovirus antibody and a TCV western North Carolina isolate, suggesting coinfection of turkeys with TCV and enterovirus in that particular case. The results indicated that the TCV isolates from these geographic locations in the U.S. shared close antigenicity and were antigenically related to IBV.  相似文献   

The effect of immunosuppression on protective immunity of turkey poults against infection with turkey coronavirus   总被引：2，自引：0，他引：2  

Loa CC  Lin TL  Wu CC  Bryan T  Hooper T  Schrader D 《Comparative immunology, microbiology and infectious diseases》2002,25(2):127-138

The objective of the present study was to evaluate the protective effect of humoral and cellular immunities on turkeys infected with turkey coronavirus (TCV). Two trials were conducted with two separate hatches of turkey poults. Turkey's were experimentally immunosuppressed with cyclosporin A (CsA) or cyclophosphamide (CY) and infected with TCV. Prior to infection, treatment with CsA selectively suppressed T cell activity as revealed by 2-3 fold decreased (p < 0.1) lymphocyte proliferation responses to a T cell mitogen, concanavalin A (Con A). Treatment with CY mainly induced B cell deficiency as indicated by significant reductions (p < 0.05) in antibody responses to sheep erythrocytes 7 days after injection. Body weight gain of turkeys treated with CY was significantly lower (p < 0.05) than that of untreated turkeys at 9 days post-infection (PI). Turkeys treated with CY had 1-2 fold higher immunofluorescent antibody assay (IFA) scores for TCV antigens (p < 0.05) in the intestine than untreated turkeys at 9 or 14 days PI. These results suggested that humoral immunity against TCV infection may be important in turkeys.  相似文献