First Established Pregnancies in Mediterranean Italian Buffaloes (Bubalus bubalis) Following Deposition of Sexed Spermatozoa near the Utero Tubal Junction     

GA Presicce  S Verberckmoes  EM Senatore  P Klinc  D Rath 《Reproduction in domestic animals》2005,40(1):73-75

At the time of AI following Ovsynch protocol, a total of 51 buffaloes were randomly divided in a first group (n = 30) subjected to conventional AI into the uterine body with 20 million non-sex sorted frozen-thawed spermatozoa, while a second group (n = 21) was inseminated near the utero-tubal junction (UTJ) ipsilateral to the ovary carrying the preovulatory follicle with 2.5 million live (4 million total) sex-sorted frozen-thawed spermatozoa. The semen used for flowcytometric sorting was collected and processed on a farm in Italy, and then shipped to a laboratory in Germany. Eleven buffaloes were inseminated with X-chromosome bearing spermatozoa and 10 with Y-chromosome bearing spermatozoa. Conception rates after conventional and UTJ inseminations were 43.3% (n = 13) and 42.8% (n = 9) respectively (p = 0.97). Eight of the nine foetuses obtained after insemination with sexed spermatozoa corresponded to the sex as predicted by the cell sorting procedure (five male and four female foetuses by ultrasound vs six male and three female foetuses by cell sorting). In conclusion, for the first time buffalo semen has been successfully subjected to procedures for flowcytometric sperm sorting and freezing. Low doses of sexed spermatozoa have been deposited near the UTJ giving conception rates similar to those of conventional AI with full dose.  相似文献   

大豆水溶性多糖的黏度性质及其应用研究     

梁建忠顾苒  高红亮孙敏 常忠义 《华北农学报》2007,22(B10):143-145

研究了不同条件下大豆水溶性多糖溶液的黏度变化情况，同时和其他常用的稳定剂如果胶等也做了比较。试验结果表明。本实验室生产的大豆水溶性多糖的水溶液的黏度随着温度的升高、浓度的降低、外加盐量的增加而降低；随着外加蔗糖量、pH值的增加而增加；相比较于其他稳定剂大豆水溶性多糖的黏度很低，使酸乳饮料具有清爽的口味。  相似文献   

防病促生枯萎病拮抗菌“98-Ⅰ”对4种枯萎病防治效果研究   总被引：3，自引：0，他引：3  

马利平  乔雄梧  高芬  郝变青 《中国生态农业学报》2005,(1)

经 8年系统试验研究从 330个细菌菌株中筛选出 1株防病促生枯萎病拮抗菌“98 Ⅰ” ,经鉴定属蜡质芽孢杆菌。该菌对黄瓜枯萎病、西瓜枯萎病、青椒枯萎病和番茄枯萎病 4种土传病害均有显著防治效果 ,其平皿孢子萌发抑制率分别为 79 2 %、75 1%、72 3%和 95 7% ,且该菌对多种蔬菜有促生和促进种子发芽功效。  相似文献   

关于开发建设项目水土保持监督管理体系的思考与建议     

高远  任明 《中国水土保持》2007,(3):31-32

把依法行政分解为法律基础和行政体系2个方面,分析了开发建设项目水土保持监督管理体系在法律基础和行政体系2个方面存在的问题。法律基础方面存在的问题:一是相关法律中提到开发建设项目水土保持监督管理的内容较少,各项职责落实不到位;二是法律中没有明确流域机构的水土保持职能。行政体系方面存在的问题有:规范化建设还不够完善,个别地方水保部门对监督管理工作内容不了解,权大于法的情况时有出现。提出了完善开发建设项目水土保持监督管理体系的建议。  相似文献   

Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs     

Robson RV Alves  Tatiana Soares  Elinaldo FL Bento  Ricardo S Roldan‐Filho  Brbara SS Souza  Marcele KN Lima  Jssica S Nascimento  Luana CBB Coelho  Roberto A S  Thmarah A Lima  Gabriel GA Gonalves  Fbio A Brayner  Luiz C Alves  Daniela MAF Navarro  Thiago H Napoleo  Patrícia MG Paiva 《Pest management science》2020,76(2):730-736

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Effects of air temperature,soil temperature and soil moisture on growth and development of tomato itself and grafted on its own and egg-plant rootstock     

A.T. Abdelhafeez  H. Harssema  K. Verkerk 《Scientia Horticulturae》1975,3(1):65-73

The problem was studied whether tomatoes, grown in a hot and arid climate, benefit from grafting on egg-plant, which is highly efficient in water uptake. Growth and development of tomato (T), tomato grafted on its own rootstock ($\text{T}\text{T}$) and tomato grafted on egg-plant rootstock ($\text{T}\text{E}$) were compared at air temperatures of 28°C during the day and 18°C during the night ($\text{28}\text{18}$) and at 28°C constantly ($\text{28}\text{28}$), at soil temperatures of 14, 21 and 28°C with the following soil moisture regimes: wet (W₁), medium (W₂) and dry (W₃).At $\text{28}\text{18}$ and $\text{28}\text{28}$ water consumption was about equal, but the transpiration ratio at $\text{28}\text{28}$ was twice as high as that at $\text{28}\text{18}$. The latter conditions gave a much stronger plant with more fruits. At a soil temperature of 14°C water use was strongly reduced. The transpiration ratio increased with the soil temperature. Differences in plant type were small. At the highest soil temperature of 28°C fruit growth was strongly reduced. At lower soil moisture levels less water was used and the transpiration was lower. Plant type was correlated herewith.Vegetative growth of $\text{T}\text{T}$ was weaker than of T, but generative growth was stimulated. The strong E rootstock stimulated vegetative growth at high air and soil temperature, but fruit growth was very poor under these conditions; at a low soil temperature of 14°C vegetative growth was also reduced.The hope that the E rootstock would be beneficial for fruit growth at high temperatures was not fulfilled.An additional experiment in a growth-room at 23°C showed that under conditions of moisture stress there was no difference in water potential between leaves of $\text{T}\text{T}$ and $\text{T}\text{E}$.  相似文献   

Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats     

HH Ortega  NR Salvetti  C Baravalle  JA Lorente  GA Mira 《Reproduction in domestic animals》2006,41(3):204-209

The objectives of this work were to determine the changes in the expression of neuroendocrine markers in Leydig cell by oestradiol treatment, and to determine whether testosterone is able to recover partially the effects of hormonal suppression induced by oestradiol. Adult male rats were injected daily with either 50 microg of oestradiol or oestradiol plus testosterone propionate (25 mg every 3 days) for 15 days. The animals were sacrificed and testicles were dissected and processed by routine histological protocols. FSH and LH serum levels were determined by radioimmunoassay. The visualization of antigens was achieved by the streptavidin-peroxidase immunohistochemical method. Antibodies against chromogranin A (CrA), S-100 protein (S-100), P substance (PS), synaptofisin (SYN), neurofilament protein (NF), gliofibrillary acidic protein (GFAP) and neuron specific enolase (NSE) were used. The mean LH and FSH serum concentrations were consistently suppressed with hormonal treatments. Intermediate filaments (NF and GFAP) showed no difference in their expression. The expression of S-100, NSE and SYN was significantly lower in both hormone-treated groups. In oestradiol-treated rats, the immunoreactivity of CrA and SP decreased significantly but was restored after testosterone supplementation. Although the nature and functions of many of these substances in Leydig cells remain unknown, these results are consistent with the hypothesis that the expression of some neuroendocrine markers is hormonally controlled.  相似文献   

Effect of Relaxin on Fertility Parameters of Frozen–Thawed Buffalo (Bubalus bubalis) Sperm          下载免费PDF全文

AR Elkhawagah  V Longobardi  G Neglia  A Salzano  G Zullo  GA Sosa  G Campanile  B Gasparrini 《Reproduction in domestic animals》2015,50(5):756-762

The aim of this work was to evaluate the effect of relaxin on fertility parameters of buffalo frozen/thawed sperm. Sperm were incubated in the absence of capacitating agents (negative control), with a known capacitating agent such as heparin (positive control) and with 50 and 100 ng/ml relaxin for 2 and 4 h. Sperm viability, motility, capacitation and the effect of relaxin on the fertilizing ability after heterologous IVF were evaluated. Although viability was not affected, relaxin increased (p < 0.05) sperm motility compared to the negative and positive controls both after 2 h (60.0 ± 2.0, 60.0 ± 3.1, 68.3 ± 1.7 and 69.4 ± 2.7, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) and 4 h (55.0 ± 2.5, 53.3 ± 3.0, 62.2 ± 3.0 and 65.0 ± 3.2, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) incubation. When sperm were incubated with both 100 ng/ml relaxin and heparin, a decrease (p < 0.01) of pattern A, that is low capacitation level, was observed compared to the negative control both after 2 h (54.4, 34.3 and 36.4%, respectively, in negative control, positive control and 100 ng/ml relaxin) and 4 h (51.9, 35.0 and 34.3%, respectively, in negative control, positive control and 100 ng/ml relaxin). Moreover, an increase (p < 0.01) of pattern EA, that is high capacitation level, was recorded with 100 ng/ml relaxin and heparin compared to the negative control both after 2 h (44.1, 59.3 and 57.7%, respectively, in negative control, positive control and 100 ng/ml relaxin) and after 4 h (43.0, 54.4 and 56.0%, respectively, in negative control, positive control and 100 ng/ml relaxin). Finally, relaxin increased (p < 0.01) cleavage rate compared to the negative control (57.1 ± 4.4, 72.5 ± 6.0, 71.4 ± 5.5 and 73.6 ± 2.9, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin). In conclusion, relaxin has a beneficial effect on motility, capacitation and fertilizing ability of frozen–thawed buffalo sperm.  相似文献   

Monensin controlled-release intraruminal capsule for control of bloat in pastured dairy cows   总被引：1，自引：0，他引：1  

LB LOWE  GJ BALL  VR CARRUTHERS†  RC DOBOS‡  GA LYNCH  PJ MOATE  PR POOLE†  SC VALENTINE 《Australian veterinary journal》1991,68(1):17-20

Monensin, a polyether ionophore antibiotic, is potentially an important agent for bloat relief in dairy cows grazing temperate legume-based pasture. A series of studies was undertaken to determine the effect of monensin, when delivered continuously in the rumen of lactating dairy cows by means of controlled-release capsules (monensin CRC). Such devices release approximately 300 mg/head/day for 100 d. A short-term pilot study made at Ruakura, New Zealand, tested monensin CRC in cows selected for high susceptibility to bloat and grazing lucerne (Medicago sativa) or red clover (Trifolium pratense). Treatment significantly reduced the incidence of bloat, while milk yield and protein yield were increased. There was no effect on fat yield. Following the pilot study, 6 large-scale field experiments involving a total of 368 lactating dairy cows, were made in Australia and New Zealand to confirm the effectiveness of monensin CRC for bloat control and to measure the effect of such treatment on milk production and composition. A severe bloat problem occurred in 2 experiments, mild bloat occurred in 2 others, while no visual signs of bloat were observed in the remaining 2 experiments. Bloat was significantly (P less than 0.05) reduced by monensin CRC treatment when data was pooled over the 4 experiments in which bloat occurred. Daily milk yield was increased in all experiments from a mean of 17.7 in untreated groups to 18.8 kg/head/day (P less than 0.05) in monensin CRC-treated cows. Protein percentage was not affected by treatment, while there was a decrease from 4.29 to 4.10% fat, although total fat yield was not affected.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

柴达木盆地弃耕地盐渍状况的主分量分析   总被引：30，自引：5，他引：25  

牛东玲  彭宏春  王启基  尕切江  李晓明  扬慧青  仓生海  祁迎林 《草业学报》2001,10(2):39-46

采用主分量分析（PCA）方法对柴达木盆地弃耕地盐渍化状况进行了研究，结果表明，该地属于中重度氯化物盐渍地、耕作层含盐量在0．5％－4．0％之间，盐分表聚强烈，基本无自然淋溶过程。耕作层第一、二主分量显著，累计贡献率高达95．0％左右，反映了该区土壤含盐量及盐离子分布规律，认为全盐量、Cl^-,Mg^2＋是该弃耕地盐渍环境的特征因子。  相似文献