The management of epilepsy in dogs     

Evans JM  Croft P 《New Zealand veterinary journal》1986,34(1-2):16

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Attitudes and perceptions of veterinary paraprofessionals in New Zealand to postoperative pain in dogs and cats     

K Kongara  HE Squance  IA Topham  JP Bridges 《New Zealand veterinary journal》2016,64(2):112-116

AIM: To survey the attitudes and perceptions of veterinary paraprofessionals in New Zealand to postoperative pain in dogs and cats.

METHODS: In December 2011, veterinary paraprofessionals (VP) from throughout New Zealand were invited to participate in an online survey. Eleven questions, which were divided into five sections, were used to determine demographic information, the respondents’ assessment of pain after commonly performed surgeries in dogs and cats, their opinions on provision of analgesia, who had responsibility for pain monitoring and the use of any formal pain scoring system in the practice.

RESULTS: Data from 165 respondents were able to be used, and 162 (98%) respondents to the survey were female. According to the respondents’ estimates, fracture repair in dogs and repair of diaphragmatic hernias in cats had the highest pain score following surgery. Neutering procedures involving dogs were scored higher than for cats (p<0.01). All respondents agreed that animals benefit from perioperative analgesia. The veterinary nurse was reported to be predominantly responsible for monitoring pain in animals postoperatively by 116/165 (70.3%) respondents. Of 165 respondents, 154 (93%) considered that their knowledge of pain and assessment of pain could be enhanced.

CONCLUSIONS: This survey reflects the attitudes and perceptions of a sample of VP in New Zealand to postoperative pain in dogs and cats. The results indicate that all respondents believe that surgery results in sufficient pain to warrant analgesic therapy. Routine neutering surgeries were considered to be more painful in dogs than in cats. The current survey also provides information to educators on potential areas of focus, given that 93% of respondents felt that their knowledge of pain and assessment of pain could be enhanced.  相似文献   

Pronuclear Embryo Yield in Canindé and Saanen Goats for DNA Microinjection     

RR Moura  ES Lopes‐Junior  DIA Teixeira  IA Serova  LE Andreeva  LM Melo  VJF Freitas 《Reproduction in domestic animals》2010,45(5):e101-e106

The objective of this study was to examine the effect of donor breed on pronuclear‐stage embryo yield to be used for DNA microinjection in a transgenesis goat program. Twelve Canindé and twelve Saanen goats were heat synchronized using a progestagen‐cloprostenol treatment. Forty‐eight hours before the sponge removal, superovulation was induced with a total administration of 4.4 mg/kg bodyweight NIH‐FSH‐P1, given twice daily in decreasing doses over 3 days. In addition, goats received 100 μg of GnRH and they were hand‐mated at 36 and 48 h after progestagen removal. Embryo recovery was performed by oviduct flushing at 72 h after sponge removal. Embryos were microinjected with a DNA construct and noticeable swelling of the nuclei was the criterion for successful microinjection. The total diameter, cytoplasm diameter, zona pellucida thickness and pronuclei diameter were measured for each microinjected embryo. A higher (p < 0.05) percentage of fertilized ova was observed in Canindé (89.9%) than Saanen (36.2%) goats. In addition, Canindé donors produced a higher percentage of pronuclear embryos when compared with Saanen: 72.5% vs 20.6% (p < 0.05), respectively. Successful microinjection was verified in 96.7% and 73.3% of times in Canindé and Saanen embryos, respectively (p < 0.05). Significant differences were observed for all morphometric parameters except for cytoplasm diameter. In conclusion, under our study experimental conditions, Canindé were more efficient than Saanen goats concerning the pronuclear embryo yield and manipulation. The use of Canindé goats in transgenesis programs could be increase the interest in their breeding and could be contribute to saving them from extinction.  相似文献   

Weed populations and pathogens   总被引：2，自引：0，他引：2  

Cousens  & Croft 《Weed Research》2000,40(1):63-82

Plant:pathogen systems are complex, highly spatial and fluctuate considerably. The dynamics of their individual populations depends on factors such as their patchiness, shapes and scales of dispersal distributions, genetic polymorphism and their responses to their biotic and abiotic environments. We review knowledge of these population properties in the context of weed management and biological control and discuss their likely influence on the population dynamics of pathogens.  相似文献   

Electroencephalography and space-occupying lesions in small animals     

Phyllis G. Croft 《The Journal of small animal practice》1972,13(4):175-184

EEGs have been recorded from 207 dogs and cats with presumed intra-cranial space-occupying lesions; twenty-seven cases were confirmed postmortem. The clinical presenting signs of the series are considered, and the abnormal characters of the EEGs are analysed and discussed. The place of the EEG in differential diagnosis and its possible future value in association with brain surgery are discussed.
Résumé. On a enregistré des électroencéphalogrammes de 207 chiens et chats suspects de lésions intracraniennes expansives; ce diagnostic a pu être confirmé par l'examen nécropsique. On décrit les manifestations cliniques majeures dans cette série et on considère les anomalies de l'E.E.G., dont on analyse les particularités et dont on envisage l'importance diagnostique sous Tangle de la chirurgie du cerveau.
Zusammenfassung. Es wurden EEGs von 207 Katzen und Hunden mit vermuteten raumbean-spruchenden Läsionen im Schädelraum aufgenommen; 27 Fälle da von wurden durch Autopsie bestätigt. Die klinischen Erscheinungsbilder der Serie werden betrachtet und der anomale Gharakter der EEGs wird analysiert und besprochen. Der Platz des EEG in der Differentialdioagnose und sein möglicher künftiger Wert in Zusammenhang mit der Gehirnchirurgie wird besprochen.  相似文献   

鸡β-防御素在大肠杆菌中的高效表达及复性   总被引：2，自引：0，他引：2  

邢海云  高英杰  宁官保  赵建增  乔彦良  李朝阳 《中国兽医学报》2007,27(2):184-187

鸡β-防御素(gallinacin-3,Gal3)是鸡体内重要的防御因子,本试验研究了鸡Gal3融合蛋白在大肠杆菌中高效表达的规律,并探索了重组蛋白质的复性工艺.结果表明,加入诱导剂后1h,开始有可检出量的重组蛋白质表达,2h的表达水平已接近最大量;化学诱导剂IPTG 0.2～1.2mol/L对重组蛋白的产量没有明显影响.经Lablmage软件分析,融合蛋白的产量可达菌体总蛋白的35%以上,且主要以包涵体形式存在.将所获包涵体用8mol/L尿素溶解后,分步稀释于含有氧化型谷胱甘肽和还原型谷胱甘肽的复性系统中,重组蛋白得到完全复性.12mg/L复性的重组鸡Gal3能抑制肠炎沙门氏菌的生长,24mg/L能抑制肠致病型大肠杆菌的生长.  相似文献   

Sensitive and specific detection of Clavibacter xyli subsp. xyli, causal agent of ratoon stunting disease of sugarcane, with a polymerase chain reaction-based assay   总被引：2，自引：0，他引：2  

M. Fegan  B. J. Croft  D. S. Teakle  A. C. Hayward  & G. R. Smith 《Plant pathology》1998,47(4):495-504

A sensitive, specific polymerase chain reaction-based assay was developed for the detection of the causal agent of ratoon stunting disease of sugarcane, Clavibacter xyli subsp. xyli . This assay uses oligonucleotide primers derived from the internal transcribed spacer region between the 16S and 23S rRNA genes of the bacterial rRNA operon. The assay is specific for C. xyli subsp. xyli and does not produce an amplification product from the template of the closely related bacterium C. xyli subsp. cynodontis , nor from other bacterial species. The assay was successfully applied to the detection of C. xyli subsp. xyli in fibrovascular fluid extracted from sugarcane and was sensitive to approximately 22 cells per PCR assay. A multiplex PCR test was also developed which identified and differentiated C. xyli subsp. xyli and C. xyli subsp. cynodontis in a single PCR assay.  相似文献   

Morphological changes of Trypanosoma vivax in mice     

M F Dirie  S L Croft  D H Molyneux 《Veterinary parasitology》1986,19(1-2):23-27

Morphological changes were observed in the blood forms of Trypanosoma vivax strain Y486 in mice on Days 12-13 of infection, following the peak parasitaemia. During this period elongate trypomastigotes, 25-40 micron long, were observed, most showing an anterior movement of the kinetoplast towards the nucleus and some having a blunt posterior end. In a few parasites a complete transformation to the epimastigote form, 40-42 micron long, was observed. Small sphaeromastigotes were also present, especially in the fine capillaries of various organs. Smears and electron microscopy suggested the presence of extra-vascular forms in the spleen.  相似文献   

Health and production of feedlot cattle following supplementation with urea–molasses in starter pens     

IA Croft  EH Clayton  PMV Cusack 《Australian veterinary journal》2014,92(5):166-170

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When fish shoals meet: outcomes for evolution and fisheries     

Darren P Croft  Jens Krause  Iain D Couzin  & Tony J Pitcher 《Fish and Fisheries》2003,4(2):138-146

The mechanisms underlying the social structure of free‐ranging fish shoals have received little attention in comparison to functional studies on shoaling. Recently, however, a number of investigations, both in the marine and in the freshwater environment, have begun to address the underlying mechanisms by concentrating on interactions between free‐ranging shoals. The rates of shoal encounters can influence the opportunities for individual assortment by phenotype and selection of shoal size; act as a constraint on the observed patterns of shoal structure by restricting individual choice behaviour and were found to be high in several small freshwater species (where intershoal distances were small), but lower in marine species where shoals were more dispersed. The duration of encounters may play a role in that it affects both the time available for assessment (of the encountered shoal) as well as that for exchange of individuals. Scarce published information on the outcome of shoal encounters suggests that the outcome of shoal encounters is influenced by shoal composition but not by shoal size. Individual behaviour may have evolved to maintain shoal size when shoals encounter, but when fish populations are depleted by fishing, this trait can exacerbate range and stock collapse. Furthermore, an understanding of the dynamics of shoal encounters has important consequences for the evolution of reciprocal altruism and the transmission of information through social learning within populations. Finally, information on encounter rates between shoals and the number of individuals that are exchanged on such occasions could be important for making predictions about the spread of disease through fish populations.  相似文献