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Flow cytometric sorting technology was used to measure the difference in DNA content between X- and Y-chromosome-bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X- and Y-chromosome-bearing sperm populations and to quantify the DNA difference between them. Two symmetrical, overlapping and clearly separated peaks, corresponding to X- and Y-bearing spermatozoa, were detected. The difference in fluorescence intensity between the peaks was 4.4 +/- 0.03% without any significant inter- or intra-animal variations. Therefore, the identification and selection of high-purity samples of sperm populations for sex sorting is easier in bucks compared with other domestic species. 相似文献
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DW Bartlett JM Clough JR Godwin AA Hall M Hamer B Parr‐Dobrzanski 《Pest management science》2004,60(3):309-309
The original article to which this Correction refers was published in Pest Management Science 58 (7): 649–662 (2002).Copyright © 2004 Society of Chemical Industry 相似文献
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Equine pituitary pars intermedia dysfunction: current understanding and recommendations from the Australian and New Zealand Equine Endocrine Group 下载免费PDF全文
CJ Secombe SR Bailey MA de Laat KJ Hughes AJ Stewart JM Sonis RHH Tan 《Australian veterinary journal》2018,96(7):233-242
The purpose of this article is to provide a review of the current knowledge and opinions about the epidemiology, clinical findings (including sequelae), diagnosis, treatment and monitoring of equine pituitary pars intermedia dysfunction, particularly in the Australian context. This information and the recommendations provided will assist practitioners in making informed decisions regarding the diagnosis and management of this disorder. 相似文献
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The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0–13 × 105 cells per flask, were challenged with SP from bulls of high or low fertility (n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr (n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high‐ or low‐fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high‐ or low‐fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose‐dependent and fertility‐dependent. 相似文献
7.
RN Zadoks E Scholz SM Rowe JM Norris HB Pooley J House 《Australian veterinary journal》2023,101(4):142-152
Numerous culture-based diagnostics are available on the Australian and international markets for on-farm detection of bacterial pathogens in milk. Use of such diagnostics may provide an opportunity to improve the prudent use of antimicrobials in udder health management. Farms are low-resource settings in terms of diagnostic microbiology capacity. The World Health Organisation has identified criteria for the evaluation of diagnostic tests in low resource settings based on Accuracy, Sensitivity, Specificity, User-friendliness, being Rapid or Robust, Equipment-free and being Deliverable (ASSURED). Here, we review how those criteria can be interpreted in the context of microbiological diagnosis of mastitis pathogens, and how on-farm diagnostics that are currently available in Australia perform relative to ASSURED criteria. This evaluation identifies multiple trade-offs, both with regard to scientific criteria and with regards to convenience criteria. More importantly, the purpose of testing may differ between farms, and test performance should be evaluated relative to its intended use. The ability of on-farm mastitis diagnostics to inform mastitis treatment decision-making in a timely and cost-effective manner depends not just on test characteristics but also on farm-specific pathogen prevalence, and on the farm enterprise's priorities and the farm manager's potential courses of action. With most assay evaluations to date conducted in professional laboratories, there is a surprising dearth of information on how well any of the diagnostic tests perform on-farm and, indeed, of the on-farm decision-making processes that they aim to inform. 相似文献
8.
In Ireland, about 90 000 ha of midland bog are being subjected to peat harvesting, principally by surface milling operations. Immediately after milling, the bog surface is free of plants and seeds. The invasion by plants of such surfaces, which have been left idle for periods of 1–12 years and which are adjacent to a peatland experimental farm, is described. The significance of such colonization for weed infestation of any subsequent crops grown on the area is inferred from data on weed proliferation on the experimental farm which has produced principally horticultural crops. Sixty-seven species of flowering plants, of which 39 are potential weeds, were recorded on the milled-over areas. This is in contrast with only 12 weed species on the experimental farm after 10 years of cropping although that number has grown to 52 species after 25 years. The farm functions as a seed source for the surrounding milled-over bog, but it is not the sole source of supply, as potential weeds (Sinapis arvensis, Veronica hederifolia) not found on the farm have been found on the bog. Wind appears to be the principal transport agency for invading species. Polygonum lapathifolium, which is dependent on flood-waters or on man for transport, is a major weed on the farm, but was not found on the milled-over bog. Tussilago farfara is much more evident on the milled areas examined than it was on the site of the farm before crop production began and than it is on the farm in 1984. Invasion de marais tourbeux par les mauvaises herbes après broyage En Irelande, environ 90 000 ha de marais au centre du pays subissent des opérations de tour-bage, surtout par broyage de la surface. Tout de suite après le broyage, la surface du marais se trouve indemne de plantes et de graines. Les auteurs décrivent l'invasion par des plantes de certaines surfaces contigues à une ferme tourbière expérimentale, après une période sans utilisation allant d'une à 12 années. A partir de données sur la prolifération des mauvaises herbes dans la ferme expérimentale, consacrée surtout à des cultures maraîcheres, ils considèrent si une telle colonisation est significative en ce qui concerne l'enherbement de cultures implantées ultérieurement sur ces surfaces. Soixante-sept espèces de plantes à fleurs, dont 39 éventuellement des adventices, ont été signalées sur les surfaces broyées. Par contre, la ferme expérimentale n'a produit que douze espèces adventices au bout de 10 ans de cultures; cependant au bout de 25 ans on a signalé 52 espèces de mauvaises herbes. La ferme fournit des graines aux marais broyés environnants mais elle n'en constitue pas la source unique, puisque certaines espèces éventuellement adventices (Sinapis arvensis, Veronica hederifolia) qu'on ne trouve pas à la ferme ont paru sur le marais. C'est surtout le vent, parait-il, qui véhicule les espèces envahissantes. Polygonum lapathifolium, espèce qui dépend d'inondations ou de l'homme pour véhiculer ses graines, est une mauvaise herbe importante à la ferme, mais n'a pas été signalé sur le marais broyé. Tussilago farfara se trouve sur les surfaces broyées en plus grand nombre que sur l'emplacement de la ferme avant l'introduction des cultures; en 1984 il est toujours moins abondant sur la ferme. Unkrautbesiedelung von oberflächlich gefrästen Torfflächen In Irland werden gegenwärtig ca. 90 000 ha Sumpfböden der mittleren Landesteile durch oberflächliche Bearbeitung mit Bodenfräsen zur Torfgewinnung genutzt. Unmittelbar nach dem Fräsen ist die Bodenoberfläche frei von Pflanzen und Samen. Die pflanzliche Besiedelung solcher, während einem bis zu zwölf Jahren sich selbst überlassenen und an eine Versuchsfarm angrenzenden Flächen ist Gegenstand vorliegender Untersuchung. Die Entwicklung der Unkrautpopulation in einer später darauf angebauten Kultur wird von der Unkrautflora der benachbarten, hauptsächlich Gartenbau betreibenden Versuchsfarm beeinflusst. 67 Blütenpflanzen, wovon 39 potentielle Unkräuter, wurden in den früher gefrästen Arealen festgestellt. Diese Beobachtung steht in Kontrast zu nur 12 Unkrautarten auf der Versuchsfarm nach zehn Jahren gärtnerischer Nutzung; allerdings stieg die Artenzahl auf 52 nach fünfundzwanzig Jahren. Die Farm wirkt als Unkrautquelle für die sie umgebenden Torfflächen; sie stellt aber nicht die einzige Quelle dar, da potentielle Unkräuter (Sinapis arvensis, Veronica hederifolia) vorgefunden wurden, die auf der Versuchsfarm fehlten. Es seheint, dass der Wind das wichtigste Transportmittel für einwandernde Arten darstellt. Polygonum lapathifolium, zur Verbreitung auf den Menschen oder fliessendes Wasser angewiesen, ist auf der Farm ein wichtiges Unkraut, wurde jedoch in der Umgebung nicht festgestellt. Tussilago farfara tritt auf den gefrästen Torfflächen viel stärker auf, als dies vor Aufnahme der gärtnerischen Produktion auf der Farm, aber auch 1984 der Fall war. 相似文献
9.
FJ Pena JA Gines J Duque V Vieitez R Martinez –Pérez L Madejón I Nuñez Martinez JM Moran S Fernández-García 《Reproduction in domestic animals》2006,41(2):189-190
A 6-year-old female Alaska Malamute dog was presented for evaluation of abdominal enlargement referred by a local veterinarian. On the history, the owner complained of chronic abdominal enlargement initiated more than 4 months ago, reduced appetite, occasional vomiting and general dullness. He also complained of greenish mucous intermittent vaginal discharge starting 10 days ago. The bitch was chronically treated with medroxiprogesterone acetate. A laparatomy was performed and fluid in the abdomen was found and aspirated during the surgery. Also a very fluid-filled distended uterus and a mass in the distal part of the left uterine horn were found. The mass was encapsulated by the omentum, but areas of necrosis and calcification were identified. Histopathological diagnosis was endometrial adenocarcinoma. 相似文献
10.
S Ikeda JM Prendes C Alonso-Montes A Rodríguez C Díez M Kitagawa H Imai E Gómez 《Reproduction in domestic animals》2006,41(5):383-385
In multiple ovulation and embryo transfer (MOET) programmes in cattle, a considerable number of morphologically poor-quality embryos continue to be produced; this is one of the limiting factors of the technique. Apoptosis has often been implicated in developmental arrest and fragmentation; these are regarded as poor traits of embryonic quality in mammalian pre-implantation embryos. In the present study, apoptosis was assessed in morphologically poor-quality embryos in comparison with good-quality embryos that were recovered from a MOET programme. Retarded embryos (two to 16 cell stage), morulae with severe fragmentation and morphologically good-quality morulae recovered from superstimulated cows at day 7 post-insemination were subjected to TdT-mediated dUTP nick-end labelling (TUNEL) and Hoechst staining. Cell nuclei that showed both TUNEL staining and apoptotic morphology were considered to be apoptotic. Apoptotic index (AI) was calculated as the percentage of apoptotic cells per embryo. Fifteen of 17 retarded embryos and 10 of 15 morphologically poor-quality morulae did not show signs of apoptosis. The mean AIs in the morphologically poor-quality embryos (two to 16 cell stage, 2.2%; poor morulae, 1.3%) were as low as that in the good-quality embryos (2.9%). These results suggest that another mode of developmental arrest and/or fragmentation that is independent of apoptosis occurs in morphologically poor-quality embryos recovered from MOET programmes. 相似文献