Pestivirus infection in a llama (Llama glama)     

Motha MX  Tham KM 《New Zealand veterinary journal》1992,40(3):126

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Production of Lambs After the Transfer of Fresh and Cryopreserved in vitro Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex-sorted Frozen-thawed Spermatozoa     

KM Morton  SL Catt  FK Hollinshead  WMC Maxwell  G Evans 《Reproduction in domestic animals》2004,39(6):454-461

Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm.  相似文献   

Isolation and identification of canine adenovirus type-2 from the upper respiratory tract of a dog     

Tham KM  Horner GW  Hunter R 《New Zealand veterinary journal》1998,46(3):102-105

AIM: To report on the isolation and identification of canine adenovirus type-2 (CAV-2) from a greyhound dog with tracheitis/tonsillitis. METHODS: Virus isolation was performed with Madin and Darby canine kidney (MDCK) cell monolayers using standard virological techniques. The isolated virus was identified by haemagglutination inhibition and serum neutralisation tests. Viral DNA was extracted from infected MDCK cells and subjected to restriction endonuclease analysis using the endonuclease enzymes Bam HI, Bgl II, Eco RI and Hind III. RESULTS: A virus, designated 5 113-87, was isolated in MDCK cells yielding typical cytopathic effect. The virus could be neutralised with a CAV-2 specific reference antiserum and also showed some cross neutralisation with CAV-1 specific reference antiserum. The virus 5 113-87 had a high haemagglutination inhibition titre with CAV-2 antiserum using human group 0 red blood-cells and CAV-1 and CAV-2 reference antisera. This virus also had DNA restriction profiles identical to those of the reference CAV-2 (Toronto A26/61), whereas previously isolated strains of adenovirus from dogs in New Zealand had DNA restriction patterns identical to the prototype CAV-1 strain (Utrecht). CONCLUSION: The findings show that the virus 5 113-87 isolated from the upper respiratory tract of a dog in New Zealand is CAV-2.  相似文献   

Device Applications of Side-Chain Ferroelectric Liquid Crystalline Polymer Films     

KM Blackwood 《Science (New York, N.Y.)》1996,273(5277):909-912

Side-chain ferroelectric liquid crystalline polymers are currently used in a number of applications, including displays and electrical sensors. Comparisons with existing technologies and materials indicate that relative to ceramics, such polymers have lower figures of merit but offer greater durability in sensor applications.  相似文献   

Resonating valence-bond ground state in a phenalenyl-based neutral radical conductor     

Pal SK  Itkis ME  Tham FS  Reed RW  Oakley RT  Haddon RC 《Science (New York, N.Y.)》2005,309(5732):281-284

An organic material composed of neutral free radicals based on the spirobiphenalenyl system exhibits a room temperature conductivity of 0.3 siemens per centimeter and a high-symmetry crystal structure. It displays the temperature-independent Pauli paramagnetism characteristic of a metal with a magnetic susceptibility that implies a density of states at the Fermi level of 15.5 states per electron volt per mole. Extended Hückel calculations indicate that the solid is a three-dimensional organic metal with a band width of approximately 0.5 electron volts. However, the compound shows activated conductivity (activation energy, 0.054 electron volts) and an optical energy gap of 0.34 electron volts. We argue that these apparently contradictory properties are best resolved in terms of the resonating valence-bond ground state originally suggested by Pauling, but with the modifications introduced by Anderson.  相似文献   

Antibody and cell-mediated immune responses to feline calicivirus following inactivated vaccine and challenge     

K M Tham  M J Studdert 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1987,34(9):640-654

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Protozoal nodular dermatitis and panniculitis in a Rottweiler puppy caused by Caryospora bigenetica          下载免费PDF全文

Heng L. Tham  Keith E. Linder  Alison Tucker  Ricardo Maggi  Petra Bizikova 《Veterinary dermatology》2016,27(1):44-e12

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Impact of Distillery Effluent on Soil Carbon and Nitrogen Dynamics in a Vertisol of Central India     

AK Biswas  Pankaj Sharma  KM Hati  S Ramana  AB Singh 《Communications in Soil Science and Plant Analysis》2018,49(17):2189-2196

Distilleries produce a huge quantity of effluents, popularly known as spent wash (SW), which when bio-methanated produce post-methanation effluents (PME). A field experiment on soybean–wheat system was conducted for five consecutive years in a Vertisol of central India to evaluate the effect of distillery effluent (DE) on soil carbon and nitrogen dynamics. Ten treatment combinations consisting of control, 100% NPK + Farmyard Manure (FYM), and graded level of SW and PME were applied. Total carbon content of soil increased significantly with applications of FYM and DE. SW was found superior in enhancing carbon content of soil in comparison to PME. Farmyard Manure contributed more carbon toward the recalcitrant pool, whereas DE contributed more carbon toward the active and slow pool. Nitrogen (N) availability was significantly improved with the application of DE. Balanced application of DE may act as amendment for increasing C and N stocks in Vertisol.  相似文献   

An outbreak of haemolytic anaemia associated with infection of Theileria orientalis in naive cattle     

McFadden AM  Rawdon TG  Meyer J  Makin J  Morley CM  Clough RR  Tham K  Mullner P  Geysen D 《New Zealand veterinary journal》2011,59(2):79-85

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Classical swine fever in Victorian domestic pigs: evidence of disease freedom     

JC Hunnam  KM Moore  P Daniel  MA Stevenson  SE Salmon 《Australian veterinary journal》2019,97(11):447-451

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