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1.
KM Morton SL Catt FK Hollinshead WMC Maxwell G Evans 《Reproduction in domestic animals》2004,39(6):454-461
Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm. 相似文献
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Kato A Higuchi Y Goto H Kizu H Okamoto T Asano N Hollinshead J Nash RJ Adachi I 《Journal of agricultural and food chemistry》2006,54(18):6640-6644
Ginger (Zingiber officinale Roscoe) continues to be used as an important cooking spice and herbal medicine around the world. Scientific research has gradually verified the antidiabetic effects of ginger. Especially gingerols, which are the major components of ginger, are known to improve diabetes including the effect of enhancement against insulin-sensitivity. Aldose reductase inhibitors have considerable potential for the treatment of diabetes, without increased risk of hypoglycemia. The assay for aldose reductase inhibitors in ginger led to the isolation of five active compounds including 2-(4-hydroxy-3-methoxyphenyl)ethanol (2) and 2-(4-hydroxy-3-methoxyphenyl)ethanoic acid (3). Compounds 2 and 3 were good inhibitors of recombinant human aldose reductase, with IC50 values of 19.2 +/- 1.9 and 18.5 +/- 1.1 microM, respectively. Furthermore, these compounds significantly suppressed not only sorbitol accumulation in human erythrocytes but also lens galactitol accumulation in 30% of galactose-fed cataract rat model. A structure-activity relationship study revealed that the applicable side alkyl chain length and the presence of a C3 OCH3 group in the aromatic ring are essential features for enzyme recognition and binding. These results suggested that it would contribute to the protection against or improvement of diabetic complications for a dietary supplement of ginger or its extract containing aldose reductase inhibitors. 相似文献
3.
试验设喷施百菌清推荐剂量(2 400g/hm2,T1)、加倍剂量(4 800g/hm2,T2)、喷清水(对照)3个处理。探讨蔬菜上百菌清残留量的安全性,开展番茄中百菌清残留的消解规律试验。结果表明,百菌清在番茄上的自然消解速度随施药剂量的增大而减慢。喷药后24h内消解最快,T1处理和T2处理施药后1d的消解率分别为60.22%和27.58%,7d的消解率达91.72%和46.67%,其半衰期分别为4.3d和18.0d。按推荐剂量施药1d后采摘,番茄中百菌清残留量为0.370mg/kg,符合国家相关标准的规定;从人体健康风险角度分析,如果按规范施药,百菌清在番茄上施用安全,产品食用无健康风险。 相似文献
4.
Separation of skin reactive intestinal cancer antigen from the carcinoembryonic antigen of Gold 总被引：1，自引：0，他引：1
A C Hollinshead C G McWright D H Alford TC GLEW P Gold R B Herbeman 《Science (New York, N.Y.)》1972,177(52):887-889
Soluble fractions of human intestinal cancer and fetal intestinal cell membranes produced delayed hypersensitivity reactions in patients with intestinal cancer. These soluble fractions and perchloric acid extracts of intestinal cancer cells were fractionated by polacrylamide-gel electrophoresis. The Gold carcinoembryonic antigen was found in a region of the gels different from that of the skin reactive antigen. 相似文献
5.
A C Hollinshead O Lee P B Chretien J L Tarpley W E Rawls E Adam 《Science (New York, N.Y.)》1973,182(113):713-715
Serums from tumor-bearing patients, cured patients, and normal subjects were examined for antibodies to the separated complement-fixing reactive components of nonvirion antigens of herpesvirus type 1 and type 2. The occurrence of antibodies to the antigens was similar in serums from tumor-bearing patients and cured patients. Antibodies to the antigens were observed among 21 of 24 (87 percent) cervical cancer cases, 44 of 49 (90 percent) laryngeal cancer cases, 15 of 24 (62 percent) cases of squamous cell carcinomas of the head and neck excluding the larynx, 2 of 24 (8 percent) nonsquamous cell cancer cases, and 3 of 51 (6 percent) normal subjects. By contrast, no differences were found in the titers of neutralizing antibodies to the virus in serums from laryngeal cancer patients and controls. The observations support an etiologic role of herpesviruses in cervical cancer and in laryngeal cancer, and possibly other squamous cell cancers of the head and neck. 相似文献
6.
Peters SO Gunn HH Imumorin IG Agaviezor BO Ikeobi CO 《Tropical animal health and production》2011,43(3):631-638
Variation in haematological parameters of Nigerian native chickens was studied using 60 clinically normal frizzle-feathered,
naked-neck, and normal-feathered native chickens. These included red blood cell count, haemoglobin, packed cell volume, white
blood cell count, mean corpuscular volume, mean corpuscular haemoglobin concentration, serum glucose, urea, cholesterol, albumin,
globulin and creatinine. Normal-feathered birds had higher (p < 0.05) mean values compared to frizzled and native neck genotypes except for albumin, red blood and white blood cells, and
mean cell haemoglobin concentration. Males generally had higher mean values than their female counterparts across all genotypes.
Correlation coefficients among the parameters were significant (p < 0.001) with r values ranging from 0.26 between red blood cell and mean corpuscular haemoglobin to 0.92 between red blood cell and cholesterol.
Sufficient genetic variation therefore exists for haematological parameters among Nigerian native chickens that may represent
indicator traits for further study. However, the application of molecular tools will provide better understanding and application
of these differences. 相似文献
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Soluble membrane antigens of lip and cervical carcinomas: reactivity with antibody for herpesvirus nonvirion antigens 总被引：6，自引：0，他引：6
With the use of antibody for herpesvirus nonvirion antigens (not structural components of the virus) complement fixing reactivity has been shown for soluble membrane antigens separated from lip and cervical carcinomas but not for similar extracts from normal vaginal tissue or intestinal carcinoma. Neither the serum obtained from the guinea pig before hyperimmunization with the herpesvirus nonvirion antigen nor the antiserum of guinea pigs immunized with comparable uninfected cell extracts reacted with these tumor soluble membrane antigens. Since the above soluble membrane antigens could be specific markers for the presence of virus genome within the tumor cells, the findings could support an etiological role of herpesvirus in selected human malignancies. 相似文献
10.
为明确小麦航天诱变后代条锈病抗性的变异和选择效果,在甘肃陇南用系谱法对8个小麦品种的诱变后代进行了研究。结果表明,兰天10号、兰天14号、兰天17号在SP1(诱变一代)和SP2(诱变二代),PASCAL和85-173-4在SP2的群体病谱均较原品种对照发生了不显著的变异,但它们在SP1和SP2都没有出现显著的条锈病抗性突变体;郑麦9023、陇原031和DORICO在SP2均出现了显著的条锈病抗性突变系且有分离现象。8个品种的SP2和SP3(诱变三代)共获得9个显著的条锈病抗性突变系和12个显著的非条锈病抗性突变系,其中15个突变系的后代都出现了条锈病抗性的分离和变异。经过4代的选择,证明感病品种在SP2出现的抗性不显著提高株系具有一定的选择可靠性,从郑麦9023和陇原031中分别获得了抗条锈、大粒和兼抗条锈、白粉病的优良突变系。这些结果表明,航天诱变小麦种子能够引起后代条锈病抗性的丰富变异;变异可能出现在SP1、SP2或以后世代;对于感病品种在SP2出现的抗性不显著提高株系可以进行选择;不论哪一方面性状的显著突变系,一般会随世代进展出现条锈病抗性的分离和变异,都应作为抗性选择的目标对象而注意保留。 相似文献