Preliminary study of plasma vascular endothelial growth factor (VEGF) during low- and high-dose radiation therapy of dogs with spontaneous tumors     

Melanie C. Wergin  Med. Vet.    Kurt Ballmer-Hofer  PhD    Malgorzata Roos  PhD    Roger E. Achermann  Med. Vet.    Natalie Inteeworn  Med. Vet.    Margarete K. Akens  Med. Vet.    Hans Blattmann  PhD    Barbara Kaser-Hotz  Dr. Med. Vet. 《Veterinary radiology & ultrasound》2004,45(3):247-254

High plasma vascular endothelial growth factor (VEGF) concentrations are associated with radiation resistance and poor prognosis. After an exposure to ionizing radiation in cell culture an early phase and a late phase of increased VEGF have been documented. The activation was dependent on the radiation dose. Therefore, the purpose of this study was to measure baseline plasma VEGF and changes in VEGF over the course of fractionated radiation therapy in dogs with spontaneous tumors. Dogs with tumors had a significantly higher pretreatment plasma VEGF than did dogs without tumors. Immediately after irradiation no increased plasma VEGF was observed. Over the course of radiation therapy there was an increased plasma VEGF in dogs treated with low doses per fraction/high total dose, whereas plasma VEGF remained stable in dogs irradiated with high doses per fraction/low total dose. The regulatory mechanisms are very complex, and therefore the value of plasma VEGF measurements as an indirect marker of angiogenesis induced by radiotherapy is limited.  相似文献   

Altered vitamin D metabolic system in follicular cysts of sows     

Malgorzata Grzesiak  Magdalena Socha  Anna Hrabia 《Reproduction in domestic animals》2021,56(1):193-196

This study aimed to examine 25OHD₃ concentration in the fluid of follicular and follicular lutein cysts of sows in comparison with preovulatory follicles as well as immunolocalize vitamin D metabolic enzymes (CYP27B1 and CYP24A1) and determine their protein abundances in the cyst wall. We have shown for the first time that 25OHD₃ level in the fluid of both cyst types was significantly lower than in preovulatory follicles. Furthermore, we have demonstrated CYP27B1 and CYP24A1 protein immunolocalization and abundance in follicular and follicular lutein cysts. The abundance of protein for both metabolic enzymes was decreased in ovarian cysts when compared to preovulatory follicles. We propose that altered VD metabolism in ovarian cyst might associate with their formation in sows.  相似文献   

Zwei Parasitenarten(Hym., Chalc., Eupelmidae undPteromalidae von Megastigmus pictus (Först.)(Hym., Chalc., Torymidae) in Lärchensamen     

Malgorzata Skrzypczynska 《Journal of pest science》1973,46(4):60-61

Zusammenfassung Auf Grund von 1968–1970 in Polen durchgeführten Untersuchungen wurden als bisher nicht bekannte Parasiten der in Lärchensamen lebendenMegastigmus pictus (Förster) (=M. seitneri Hoffm.) (Hymen., Torymidae) die ChalcidoidenEupelmus urozonus Dalm. (Hymen., Eupelmidae) undMesopolobus zetterstedtii (Dalla Torre) (Hymen., Pteromalidae) nachgewiesen.Die Zuchten aus Samen und Zapfen von 69 polnischen Oberförstereien (104 Standorte) zeigten, daß die beiden Parasiten eine beträchtliche Rolle bei der Einschränkung der Population vomMegastigmus pictus spielen können. Die Untersuchungen bilden die Grundlage für einen eventuellen Einsatz beider Parasitenarten zur biologischen Bekämpfung des Samenschädlings.

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Summary The autor demonstrates, as a result of investigation carried out in the years 1968–1970, thatEupelmus urozonus Dalm. (Hymen., Eupelmidae) andMesopolobus zetterstedtii (Dalla Torre) (Hymen., Pteromalidae) being parasites ofMegastigmus pictus (Förster) (=M. seitneri Hoffm.) (Hymen., Torymidae), an insect damaging the seeds of the European and Polish larch.It was stated from 69 forest districts (104 localities) in the area of Poland, that these parasites are able to play a considerable role in the limitation ofMegastigmus pictus populations. An indication of the se parasites may presage the possibility of a biological restriction of damages caused by this harmful insect.

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The Effect of Consumption of Selenium Enriched Rye/Wheat Sourdough Bread on the Body’s Selenium Status     

Bryszewska MA  Ambroziak W  Langford NJ  Baxter MJ  Colyer A  Lewis DJ 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(3):121-126

The potential of selenium-enriched rye/wheat sourdough bread as a route for supplementing dietary selenium intakes is reported. In addition to their normal diets, 24 female volunteers (24 to 25 years old) were fed either selenium-enriched bread or non-enriched bread each day (68.02 and 0.84 μg selenium day⁻¹ respectively) for 4 weeks. The chemical form of the selenium in the bread had been characterised using HPLC-ICP-MS, which showed that 42% of the extractable selenium was present as selenomethionine. Plasma selenium levels and plasma platelet glutathione peroxidase (GPx1) activity were measured in the volunteers’ blood over a 6-week period. A statistically significant difference (p = 0.001) was observed in the mean percentage change data, calculated from the plasma selenium level measurements for the enriched and control group, over the duration of the study. A comparable difference was not observed for the platelet GPx1 activity (p = 0.756), over the same period. Two weeks after cessation of the feeding stage, i.e., at t = 6 weeks, the mean percentage change value for the selenium plasma levels in the enriched group was still significantly elevated, suggesting that the absorbed selenium had been incorporated into the body’s selenium reserves, and was then being slowly released back into the volunteers’ blood.  相似文献   

Reaction of Oilseed Rape Cultivars to Sclerotinia Stem Rot in Field Environments     

SUN Jun-Ming  HAN Fen-Xia  Malgorzata Jedryczka 《中国农业科学(英文版)》2005,4(4)

Sclerotinia sclerotiorum (Lib.) de Bary, as an ubiquitous phytopathogenic Ascomycete fungus capable of infecting a wide range of plants, has increased in importance for oilseed rape production worldwide in recent years. The objective of this research was to determine the effectiveness of two measurements of disease rating in predicting the field reaction of cultivars to sclerotinia stem rot. One hundred and ten cultivars or lines of oilseed rape were repeatedly evaluated the disease severity index (DSI) and lesion length for their resistance to nine isolates of S. sclerotiorum in two field stations.There were greatly significant differences between two stations, among isolates, among population of cultivars for the disease severity index and lesion length, and also significant station×isolate interaction for lesion length and population×isolate interactions for DSI and lesion length, but interactions of station×isolate for DSI were not significant.Cultivars such as Pomorzanin and Lisek consistently had the lowest disease severity ratings in the field tests. However,most of cultivars were susceptible to sclerotinia stem rot in the field test. A positive correlation between the mean DSI and lesion length was found for both isolates (Sc23 and B1). The correlations ranged from 0.23 to 0.83 at various plots, with nine of the twelve correlations significant. The correlations for disease caused by different fungal isolates were statistically significant difference. These correlations ranged from -0.44 to 0.90, with nine of the thirty-two correlations significant. It indicated that resistance to some isolates was significantly correlated and selection of some varieties for resistance to one isolate appeared to improve resistance to other isolates.  相似文献   

Human chromosome 7: DNA sequence and biology     

Scherer SW  Cheung J  MacDonald JR  Osborne LR  Nakabayashi K  Herbrick JA  Carson AR  Parker-Katiraee L  Skaug J  Khaja R  Zhang J  Hudek AK  Li M  Haddad M  Duggan GE  Fernandez BA  Kanematsu E  Gentles S  Christopoulos CC  Choufani S  Kwasnicka D  Zheng XH  Lai Z  Nusskern D  Zhang Q  Gu Z  Lu F  Zeesman S  Nowaczyk MJ  Teshima I  Chitayat D  Shuman C  Weksberg R  Zackai EH  Grebe TA  Cox SR  Kirkpatrick SJ  Rahman N  Friedman JM  Heng HH  Pelicci PG  Lo-Coco F  Belloni E  Shaffer LG  Pober B  Morton CC  Gusella JF  Bruns GA  Korf BR 《Science (New York, N.Y.)》2003,300(5620):767-772

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Species-specific sensitivity of earthworm coelomocytes to dermal metal (Cd,Cu, Ni,Pb, Zn) exposures: Methodological approach     

Barbara Plytycz  Malgorzata Klimek  Joanna Homa  Agnieszka Irena Mazur  Jerzy Kruk  A. John Morgan 《Pedobiologia》2011

Earthworm immune-competent cells (coelomocytes) can be adversely affected by soil metal contamination. The aim of the present paper was to perform comparative studies on the coelomocytes of four lumbricid species dermally exposed for 2 days to filter paper soaked with Ni, Cu, Zn, Cd, or Pb chlorides (1 mM). Coelomocytes were expelled by electro-stimulation, counted, and assayed ex vivo by flow cytometry and spectrofluorometry. The coelomocytes of Allolobophora chlorotica and Dendrobaena veneta contain both amoebocytes and riboflavin-storing eleocytes; consequently, they were subjected to assays based on flow cytometric quantification of autofluorescent eleocytes and spectrofluorometric measurement of riboflavin content in coelomocyte lysates. In Al. chlorotica, the number of coelomocytes, the percentage of eleocytes, and the amount of riboflavin were significantly lower in Cu-exposed worms although these cytometric parameters were less affected by Ni, Zn, Cd exposure, and entirely unaffected by Pb exposure. In D. veneta, such cellular effects were also only observed in Cu-exposed worms. The coelomic fluids of Aporrectodea caliginosa and Lumbricus rubellus are constitutionally impoverished of eleocytes; therefore, these species could not be subjected to assays measuring the riboflavin content of these granular cells. Rather, the amoebocytes of Ap. caliginosa and L. rubellus were subjected to flow cytometric measurements of in vitro neutral red uptake (NR). However, the NR uptake assay was technically demanding, requiring a strictly normalized incubation period over all samples to yield useful comparative data. In contrast, the riboflavin content in the coelomocyte lysates of eleocyte-rich species appears to be a robust and convenient immune-function biomarker of environmental stress.  相似文献   

Riboflavin storage in earthworm chloragocytes and chloragocyte-derived eleocytes and its putative role as chemoattractant for immunocompetent cells     

Agnieszka Irena Mazur  Malgorzata Klimek  A. John Morgan  Barbara Plytycz 《Pedobiologia》2011

We have shown previously that riboflavin is stored in chloragosomes of chloragocyte-derived earthworm eleocytes what may have an adaptive value for worms vulnerable to soil-derived pathogen invasion. Thus, it is important to determine whether riboflavin stores are also present in earthworm species with low eleocyte counts, especially in the chloragocytes of their chloragogen tissue that surrounds the coelomic site of the intestine, and find out its putative roles in earthworm immunity. Experiments were performed on adult worms from 6 species. Freely floating coelomocytes (consisting of amoebocytes plus species-specific numbers of eleocytes) were extruded by mild electric shock and then chloragocytes from the same anaesthetized worm were mechanically detached into buffer. Both cell suspensions were analyzed by spectrofluorometry for riboflavin content. It turned out that riboflavin is stored in species-specific quantities in all investigated species. Riboflavin storage predominates in free coelomocytes of eleocyte-rich species (Eisenia andrei, Dendrobaena veneta, Allolobophora chlorotica) while in chloragocytes of species with few eleocytes (Lumbricus terrestris, L. rubellus, and Aporrectodea caliginosa). Upon a massive microbial impact, the coelomocytes (both amoebocytes and eleocytes) are involved in the formation of multicellular bodies encapsulating soil-derived pathogens, what is connected with enhanced coelomocyte mobility. Thus the second aim of the present investigation was to check if riboflavin can participate in coelomocyte accumulations. Tests performed in a 48-well chemotaxis chamber revealed that riboflavin behave as chemoattractant for coelomocytes of all investigated earthworm species, which may be one of mechanisms underpinning its immunomodulatory functions.  相似文献   

Influence of Soil Drought on Plant Growth, Assimilation and Dissimilation of CO₂ and Accumulation of Photosynthates in Field Bean (Vicia faba L. minor) During Pod Formation and Rapid Pod Growth     

J. Kocielniak  W. Filek  S. Grzesiak 《Journal of Agronomy and Crop Science》1989,163(5):330-337

Field bean planes cultivar Nadwiślański were submitted to soil drought (30 % of field soil water capacity) for 5 days at the stage of pod formation (A) and of rapid pod growth (B) and then exposed for 20 minutes to ¹⁴CO₂. Radioactivity of leaves, stems, roots, and pods or pod shells and seeds was measured 1, 5, 24 and 48 hours after exposition.
In both stages soil drought reduced by about five times total CO₂ assimilation, mainly owing to lower activity of the photosynthetic apparatus and also, though less so, to reduced leaf growth. Photosynthetic activity referred to the dry weight of the leaves dropped to 22-35% of controls. Accumulation of photosynthetates in generative organs was much less depressed than ¹⁴CO₂ assimilation. 48 hours after exposition to ¹⁴CO₂ of drought treated plants, the contents of ¹⁴C of pods in phase A, and seeds in phase B, amounted to respectively 24% and 36% of assimilated ¹⁴C and equalled 91.5% and 74% of the corresponding values for controls.
The progressive decline of radioactivity in leaves and stems after ¹⁴CO₂ exposition was distinctly correlated to the rise of radioactivity of generative organs both in soil drought treated plants and in controls. Slightly lower values of correlation coefficients in drought treated plants may indicate impairment under drought conditions of synchronization in processes of unloading and accumulation of assimilates.
In plants drought treated in phase A the ability to dissimilate ¹⁴C was reduced to about 59% of that in controls, but when drought was applied in phase B, dissimilation rate was about three times as high.  相似文献   

Effects of Soil Drought in the Generative Phase of Development of Field Bean (Vicia faba L. var. minor) on Leaf Water Status, Photosynthesis Rate and Biomass Growth     

S. Grzesiak    J. Koscielniak    W. Filek  G. Augustyniak 《Journal of Agronomy and Crop Science》1989,162(4):241-247

The effects of 10-day periods of soil drought during flowering (DI) or pod growth (DII) and during both these phases (DI + DII) on leaf water status, photosynthesis rate, plant growth and production of biomass was studied in pot experiments with field bean. Soil water content during drought spells were kept at 30% of field water capacity. Soil water deficiency DI and DII depressed the leaf water potential relatively to control by about 100 % and 140 % respectively, increased the water deficit by 100 % and 120 % and reduced photosynthesis rate by 80 % and 90 %. Rehydration of tissues completely abolished any decline of photosynthesis rate caused by drought DI, but after drought DII photosynthesis rate remained depressed by about 30 % even after rehydration. Drought periods DI and DII caused more rapid withering of leaves, a decline in the total leaf area and a temporary increase of root weight.
Drought occuring at flowering (DI) also activated the mechanism of plant adaptation to water deficiency during the pod formation phase (DII). Single or double periods of drought had not any significant influence on the number of pods and seeds, but decreased their weight.  相似文献