Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts          下载免费PDF全文

TC Marques  EC da Silva Santos  TO Diesel  LO Leme  CF Martins  MAN Dode  BG Alves  FPH Costa  EB de Oliveira  ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236

Effects of adding different concentrations of melatonin (10^?7, 10^?9 and 10^?11 M) to maturation (Experiment 1; Control, IVM  + 10^?7, IVM  + 10^?9, IVM  + 10^?11) and culture media (Experiment 2; Control, IVC  + 10^?7, IVC  + 10^?9, IVC  + 10^?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10^?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM  + 10^?9, IVC  + 10^?9, IVM /IVC  + 10^?9). In Experiment 1, maturated oocytes from Control and IVM  + 10^?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC  + 10^?7 (43.5%; 56.7%) and IVC  + 10^?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC  + 10^?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC  + 10^?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC  + 10^?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC  + 10^?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM  + 10^?9. Reactive oxygen species production was greater in the IVM /IVC  + 10^?9 treatment group. In conclusion, the 10^?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.  相似文献   

The Pinewoods and human use, 1600-1900     

Smout  TC 《Forestry》2006,79(3):341-349

Human use of the pinewoods in 1600–1900 is briefly reviewed,and related to evidence for differing woodland structure inthese centuries. A certain number of pinewoods were lost between1600 and 1800 and it is suggested that this may be related toclimate change.  相似文献   

Cross-protective immunity and the serological classification system for Bacteroides nodosus     

DJ STEWART  JA VAUGHAN  TC ELLEMAN†  PA HOYNE†  KJ BURNS  JH DUFTY 《Australian veterinary journal》1991,68(2):50-53

The relationship between the serological classification system for serogroup B and for serogroup H of Bacteroides nodosus and cross-protection between subgroups within these serogroups was examined. Protection against ovine footrot following vaccination was achieved against other subgroup strains provided sufficient cross-reactive antibody was induced by shared pilus antigens. Within serogroup B, better cross-protection against one subgroup was obtained with a pili vaccine than a whole cell vaccine which correlated with higher pilus antibody titres induced by the former. For serogroup H, a lack of cross-protection and serological reactivity between subgroups was demonstrated, which indicates that the prototype strain of subgroup H2 should be designated a new serogroup.  相似文献   

The effect of an extended artificial photoperiod and gonadotrophin-releasing hormone infusions in inducing fertile oestrus in anoestrous mares     

TC LOWIS  JH HYLAND†† 《Australian veterinary journal》1991,68(12):400-402

The occurrence of fertile oestrus early in the breeding season is of paramount importance to the Thoroughbred industry to facilitate early conception. This paper compares 2 techniques for inducing fertile oestrus in anoestrous mares using either an extended photoperiod alone or together with gonadotrophin-releasing hormone (GnRH) infusions. Eleven mares were placed under conditions of 16 h light and 8 h darkness and 5 of these were implanted with osmotic minipumps delivering approximately 100 ng GnRH/kg/h for 28 days (treated mares). The treated mares ovulated 27.7 days earlier than and conceived 32 days earlier than the 6 mares not given GnRH. GnRH-induced ovulations were followed by a competent luteal phase. The combination of GnRH pumps implanted 2 weeks before commencement of service together with extended photoperiod from July 1 has promise in assisting the stud breeder to improve reproductive efficiency on commercial stud farms.  相似文献   

A Chemoautotrophically Based Cave Ecosystem   总被引：1，自引：0，他引：1  

SM Sarbu  TC Kane  BK Kinkle 《Science (New York, N.Y.)》1996,272(5270):1953-1955

Microbial mats discovered in a ground-water ecosystem in southern Romania contain chemoautotrophic bacteria that fix inorganic carbon, using hydrogen sulfide as an energy source. Analysis of stable carbon and nitrogen isotopes showed that this chemoautotrophic production is the food base for 48 species of cave-adapted terrestrial and aquatic invertebrates, 33 of which are endemic to this ecosystem. This is the only cave ecosystem known to be supported by in situ autotrophic production, and it contains the only terrestrial community known to be chemoautotrophically based.  相似文献   

Compressibility of M3C60 Fullerene Superconductors: Relation Between Tc and Lattice Parameter     

Zhou O  Zhu Q  Fischer JE  Coustel N  Vaughan GB  Heiney PA  McCauley JP  Smith AB 《Science (New York, N.Y.)》1992,255(5046):833-835

X-ray diffraction and diamond anvil techniques were used to measure the isothermal compressibility of K(3)C(60) and Rb(3)C(60), the superconducting, binary alkali-metal intercalation compounds of solid buckminsterfullerene. These results, combined with the pressure dependence of the superconducting onset temperature T(c) measured by other groups, establish a universal first-order relation between T(c) and the lattice parameter a over a broad range, between 13.9 and 14.5 angstroms. A small secondorder intercalate-specific effect was observed that appears to rule out the participation of intercalate-fullerene optic modes in the pairing interaction.  相似文献   

Shocked quartz at the triassic-jurassic boundary in Italy     

Bice DM  Newton CR  McCauley S  Reiners PW  McRoberts CA 《Science (New York, N.Y.)》1992,255(5043):443-446

Quartz grains that appear to have been shock-metamorphosed occur within three closely spaced shale beds from the uppermost Triassic ("Rhaetian") Calcare a Rhaetavicula in the Northern Apennines of Italy. The upper shale coincides with the abrupt termination of the distinctive, uppermost Triassic Rhaetavicula fauna and is overlain by the Hettangian (Lower Jurassic) Calcare Massiccio; no extinctions appear to be associated with the two lower layers, which occur 1.2 and 2.4 meters below the boundary shale. Approximately 5 to 10% of the quartz grains within these layers exhibit one or more sets of planar deformational features whose orientations cluster around the rational crystallographic planes (basal, omega, and pi) most commonly observed in shocked quartz. Textural and stratigraphic observations support an interpretation of at least three closely spaced impacts at the end of the Triassic.  相似文献   

X-ray diffaction evidence for nonstoichiometric rubidium-c60 intercalation compounds     

Zhu Q  Zhou O  Coustel N  Vaughan GB  McCauley JP  Romanow WJ  Fischer JE  Smith AB 《Science (New York, N.Y.)》1991,254(5031):545-548

Powder x-ray diffraction at 300 K on equilibrated samples of several nominal compositions chi in Rb(chi)C(60) is reported. In addition to the face-centered cubic (fcc) (chi = 3, superconducting), body-centered tetragonal (chi = 4), and body-centered cubic (bcc) (chi = 6) stoichiometric phases, direct evidence for a dilute fcc doped phase, 0 x c 相似文献   

Fishing for good news     

McCauley DJ 《Science (New York, N.Y.)》2007,316(5822):200-1; author reply 200-1

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Shock-induced localized amorphization in boron carbide     

Chen M  McCauley JW  Hemker KJ 《Science (New York, N.Y.)》2003,299(5612):1563-1566

High-resolution electron microscope observations of shock-loaded boron carbide have revealed the formation of nanoscale intragranular amorphous bands that occur parallel to specific crystallographic planes and contiguously with apparent cleaved fracture surfaces. This damage mechanism explains the measured, but not previously understood, decrease in the ballistic performance of boron carbide at high impact rates and pressures. The formation of these amorphous bands is also an example of how shock loading can result in the synthesis of novel structures and materials with substantially altered properties.  相似文献