COLLAGENASE CHEMONUCLEOLYSIS—A LONG TERM RADIOGRAPHIC STUDY IN NORMAL DOGS     

Matthew A.O.  Atilola  DVM  PhD  Joanne R.  Cockshutt  DVM  MS  Ron  Mclaughlin  DVM  DVSc  Susan M.  Cochrane  DVM  DVSc  Paul W.  Pennock  DVM  MS 《Veterinary radiology & ultrasound》1993,34(5):321-324

Five clinically normal five year old dogs were used in this study. From a randomised table inter ver tebral discs were each injected with either collagenase or calcium chloride diluent. The surgically exposed cervical discs were injected with 50 units whereas thoracic and lumbar discs were injected under fluoroscopic guidance with 100 units of the enzyme. Postinjection radiographs revealed significant (p ≤ .05) disc space narrowing in enzyme injected discs. The cervical discs had the highest frequency of radiographic narrowing (87%) followed by the thoracic (70%) and lumbar (53%) discs. Spondylosis deformans developed at the sites of cervical enzyme injections. None of the dogs had neurologic abnormalities one year postinjection.  相似文献   

A Prospective Study Of Intravenous Catheter Contamination     

Karol A. Mathews DVM  DVSc  Melanie J. Brooks RVT  Anne E. Valliant BSC 《Journal of Veterinary Emergency and Critical Care》1996,6(1):33-43

A one year prospective study was conducted to determine the association between intravenous catheter contamination and increased dwell time, and to identify any related risk factors. Intravenous catheters obtained from 23 cats and 98 dogs in the Intensive Care Unit at the Ontario Veterinary College with dwell times > 72 hours for the test group (n=58) and < 72 hours for a corresponding control group (n=63) were cultured between April 1991 and March 1992. One hundred and twenty one catheters were cultured, 16 jugular, 99 cephalic, and 6 saphenous. The overall contamination rate was 13 out of 121 catheters cultured (10.7%); 9/63 (14.3%) control and 4/58 (6.9%) test catheters. The bacteria isolated were E.aerogenes, S.aureus (3), P.aeruginosa, P.multocida, and Bacillus sp (7). The Bacillus sp positive catheters (5 control and 2 test) were placed during a five day period, and contaminated gauze squares were identified as the source of infection in these catheters. After these were removed from the study, the group infection rate was 6.9% control and 3.6% test. There was no significant difference between groups and no associated risk factors were identified. We conclude that intravenous dwell time need not be restricted to <72 hours.  相似文献   

ULTRASONOGRAPHIC FEATURES OF DIFFUSE HEPATOCELLULAR DISORDERS IN DAIRY CATTLE     

Jezie A.  Acorda  DVM  Magr  Haruo  Yamada  DVM  DVSc  Seyed  Mehdi Ghamsari  DVM 《Veterinary radiology & ultrasound》1994,35(3):196-200

Ultrasonography of the liver was performed in 200 Holstein-Friesian dairy cattle using a 3.5 MHz transducer with a linear array electronic scanner. Liver samples were taken, processed and examined microscopically and the fat occupying rate was calculated. The hepatic ultrasonograms were evaluated according to the presence of bright pattern, dark pattern, deep attenuation, vascular blurring and blurring of edges. Of the 200 animals, 96 had a normal liver, 63 had hydropic degeneration of the liver, 37 had fatty infiltration of the liver, 3 had liver dystrophy and I had hepatic amyloidosis, diagnosed through histopathological examination. Amyloidosis was characterized by bright pattern and blurring of edges. Liver dystrophy had higher percentages of bright pattern and blurring of edges than normal liver. Hydropic degeneration had higher percentages of dark pattern and blurring of edges than normal liver. Fatty infiltration had higher percentages of bright pattern, deep attenuation, vascular blurring and blurring of edges than normal liver. The present results suggest that different ultrasonographic patterns can be observed in various diffuse hepatocellular disorders in dairy cattle  相似文献   

Avian care and husbandry.     

Michelle S Schulte  Agnes E Rupley 《Veterinary Clinics of North America: Exotic Animal Practice》2004,7(2):315-50, vi-vii

Birds are becoming more popular as pets, and the need for veterinary professionals to learn more about these pets is crucial. This article introduces the veterinary staff to basic information required to provide high-quality care to avian patients. High-quality nutrition for a variety of species and ages is included in this article. The importance of proper husbandry and intellectual stimulation with toys is also discussed. Special emphasis is placed on instructions for clients when making appointments, as well as recognizing signs of illness for both clients and veterinary staff. Capture, restraint, and sample collection are an important part of this article, as well as medication administration, anesthesia, supportive care, preventive care and grooming. A variety of aspects concerning the care of the avian patient are covered for the benefit of the client and veterinary technician.  相似文献   

Catheter‐associated venous air embolism in hospitalized horses: 32 cases          下载免费PDF全文

Nicholas J. Parkinson  Harold C. McKenzie  Michelle H. Barton  Jennifer L. Davis  Bettina Dunkel  Amy L. Johnson  Elizabeth S. MacDonald 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(2):805-814

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In vitro evaluation of a simulated pneumoperitoneum environment using carbon dioxide on canine transitional cell carcinoma          下载免费PDF全文

Rashi Asthana MSc  Michelle L. Oblak DVM  DVSc  Diplomate ACVS  ACVS Fellow of Surgical Oncology  Ameet Singh DVM  DVSc  Diplomate ACVS 《Veterinary surgery : VS》2018,47(3):412-420

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Brewery residues as a source of organic carbon in Litopenaeus schmitti white shrimp farms with BFT systems     

Michelle Midori Sena Fugimura  Helaine dos Reis Flor  Emanuela Paula de Melo  Tiago Viana da Costa  Wilson Wasielesky Jr.  Lidia Miyako Yoshii Oshiro 《Aquaculture International》2015,23(2):509-522

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The effects of flumazenil on ventilatory and recovery characteristics in horses following midazolam-ketamine induction and isoflurane anaesthesia     

Hope Douglas  Klaus Hopster  Michelle Cerullo  Charlotte Hopster-Iversen  Darko Stefanovski  Bernd Driessen 《Equine veterinary journal》2021,53(6):1257-1267

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Randomly primed,strand-switching,MinION-based sequencing for the detection and characterization of cultured RNA viruses     

Kelsey T. Young  Kevin K. Lahmers  Holly S. Sellers  David E. Stallknecht  Rebecca L. Poulson  Jerry T. Saliki  Stephen Mark Tompkins  Ian Padykula  Chris Siepker  Elizabeth W. Howerth  Michelle Todd  James B. Stanton 《Journal of veterinary diagnostic investigation》2021,33(2):202

RNA viruses rapidly mutate, which can result in increased virulence, increased escape from vaccine protection, and false-negative detection results. Targeted detection methods have a limited ability to detect unknown viruses and often provide insufficient data to detect coinfections or identify antigenic variants. Random, deep sequencing is a method that can more fully detect and characterize RNA viruses and is often coupled with molecular techniques or culture methods for viral enrichment. We tested viral culture coupled with third-generation sequencing for the ability to detect and characterize RNA viruses. Cultures of bovine viral diarrhea virus, canine distemper virus (CDV), epizootic hemorrhagic disease virus, infectious bronchitis virus, 2 influenza A viruses, and porcine respiratory and reproductive syndrome virus were sequenced on the MinION platform using a random, reverse primer in a strand-switching reaction, coupled with PCR-based barcoding. Reads were taxonomically classified and used for reference-based sequence building using a stock personal computer. This method accurately detected and identified complete coding sequence genomes with a minimum of 20× coverage depth for all 7 viruses, including a sample containing 2 viruses. Each lineage-typing region had at least 26× coverage depth for all viruses. Furthermore, analyzing the CDV sample through a pipeline devoid of CDV reference sequences modeled the ability of this protocol to detect unknown viruses. Our results show the ability of this technique to detect and characterize dsRNA, negative- and positive-sense ssRNA, and nonsegmented and segmented RNA viruses.  相似文献   

Stress steroid levels and the short-term impact of routine dehorning in female southern white rhinoceroses (Ceratotherium simum simum)     

Marcha Badenhorst  Michelle Otto  Annemieke C van der Goot 《African Zoology》2016,51(4):211-215

Rhinoceros populations in Africa are under severe threat as a result of surging poaching rates and risk-mitigation strategies are continuously adapted in an attempt to ensure the survival of the species. This study compared faecal glucocorticoid metabolite (fGCM) levels of two age classes of limited free-ranging female white rhinos with fGCM levels of adult free-ranging female white rhinos. Subsequently, fGCM alterations in the limited free-ranging animals were monitored following routine dehorning as a measure of the animals’ short-term physiological stress response. Baseline fGCM levels differed significantly between tested groups, with both free-ranging and limited free-ranging adult animals showing significantly higher fGCM levels compared with limited free-ranging juvenile females. In contrast, baseline fGCM levels did not differ significantly between limited free-ranging and free-ranging adult individuals. Routine dehorning procedures resulted in a short-term stress response expressed by a significant increase in fGCM levels 48 h post-dehorning, with stress steroid levels returning to pre-dehorning concentrations 72 h after the procedure.  相似文献