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1.
Hiroshi UENO Osamu YAMATO Takeshi SUGIURA Moeko KOHYAMA Akira YABUKI Kenjiro MIYOSHI Kazuya MATSUDA Tsuyoshi UCHIDE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):91-95
A male Japanese domestic cat with retarded growth in Hokkaido, Japan, showed
progressive motor dysfunction, such as ataxia starting at 3 months of age and tremors,
visual disorder and seizure after 4 months of age. Finally, the cat died of neurological
deterioration at 9 months of age. Approximately half of the peripheral blood lymphocytes
had multiple abnormal vacuoles. Magnetic resonance imaging showed bisymmetrical
hyperintensity in the white matter of the parietal and occipital lobes in the forebrain on
T2-weighted and fluid-attenuated inversion recovery images, and mild encephalatrophy of
the olfactory bulbs and temporal lobes. The activity of lysosomal acid β-galactosidase in
leukocytes was negligible, resulting in the biochemical diagnosis of GM1 gangliosidosis.
Histologically, swollen neurons characterized by accumulation of pale, slightly granular
cytoplasmic materials were observed throughout the central nervous system. Dysmyelination
or demyelination and gemistocytic astrocytosis were observed in the white matter.
Ultrastructually, membranous cytoplasmic bodies were detected in the lysosomes of neurons.
However, genetic analysis did not identify the c.1448G>C mutation, which is the single
known mutation of feline GM1 gangliosidosis, suggesting that the cat was affected with a
new variant of the feline disease. 相似文献
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Kazuya KUSHIDA Urs GIGER Toshihiko TSUTSUI Megumi INABA Yoshio KONNO Kureha HAYASHI Kana NOGUCHI Akira YABUKI Keijiro MIZUKAMI Moeko KOHYAMA Yasuyuki ENDO Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):743-746
Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy
caused by mutations of the PKLR gene. A causative mutation of the feline
PKLR gene was originally identified in Abyssinian and Somali cats in
the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was
developed and evaluated for rapid genotyping and large-scale screening for this mutation.
Furthermore, a genotyping survey was carried out in a population of four popular purebred
cats in Japan to determine the current mutant allele frequency. The assay clearly
displayed all genotypes of feline PK deficiency, indicating its suitability for
large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele
frequency in Abyssinian and Somali cats was high enough to warrant measures to control and
prevent the disease. The mutant allele frequency was relatively low in Bengal and American
Shorthair cats; however, the testing should still be carried out to prevent the spread of
the disease. In addition, PK deficiency should always be considered in the differential
diagnosis of anemia in purebred cats in Japan as well as worldwide. 相似文献
4.
The effects of blending rice flour with wheat flour on bread texture and staling were investigated with three rice varieties with different amylose contents. For the texture analysis of bread crumb, the compression test, puncture test, and tensile test were performed. A flour blend containing rice flour suppressed the recovery of the crumb after compression. For the puncture test, blends with rice flour increased the distance to penetrate the crumb. The rupture strain measured with the puncture test decreased with staling of the bread crumb for all samples tested. The fresh bread crumb sample containing waxy rice flour had much greater extensibility in the tensile test than the other samples tested, but it was dramatically decreased after one day of storage. Endothermic enthalpies corresponding to retrograded amylopectin, which is part of the staling process, were also measured. The enthalpy of bread crumb from the blended flour was lower than that of wheat flour bread up to three days but was higher on day 4. A blend of rice flour thus reduced amylopectin retrogradation during early storage, but it was accelerated later. Bread blended with waxy rice flour showed the lowest enthalpy during storage. 相似文献
5.
The rheological properties of cooked white salted noodles made from eight wheat cultivars with varied amylose content were analyzed at small and large deformation. Their dynamic shear viscoelasticity was measured using a rheometer with parallel plate geometry. Compressive force and creep‐recovery curves were measured using various probes and sample shapes. Noodles with lower amylose content showed a lower storage shear modulus (G′) and a higher frequency dependence of G′. The G′ values of noodles were highly correlated with amylose content in wheat flour and with G′ values of 30 and 40% starch gels. Remarkable differences in the characteristics of creep‐recovery curves were observed between cultivars. The difference in amylose content in wheat flour reflected the creep‐recovery properties of noodles. A negative correlation was demonstrated between amylose content and both maximum creep and recovery compliance. The compressive force required for 20, 50, 80, and 95% strains was compared. At 20 and 50% strain, noodles made from lower amylose wheat flour showed lower compressive force. Noodles of waxy wheat had a higher compressive force than nonwaxy noodles when the strain was >80%, indicating the waxy wheat noodles are soft but difficult to completely cut through. 相似文献
6.
Distribution and stem growth patterns of mangrove species along the Nakara River in Iriomote Island, Southwestern Japan 总被引：1，自引：0，他引：1
Tsutomu Enoki Moeko Ueda Daisuke Nanki Rempei Suwa Akio Hagihara 《Journal of Forest Research》2009,14(1):51-54
We examined variations in stand structure and tree growth of a mangrove forest along two gradients: from the river mouth to
upper stream and from the riverside to inland, along the Nakara River, Iriomote Island, Japan. Bruguiera gymnorrhiza (L.) Lamk. occurred throughout the intertidal area, though Rhizophora stylosa Griff. and Kandelia candel (L) Druce did not occur upstream. Basal area and maximum tree height of B. gymnorrhiza decreased downstream. The growth rate in stem diameter of B. gymnorrhiza decreased downstream in relation with the soil salinity. The basal area and the maximum tree height of B. gymnorrhiza increased with the distance from the riverside. R. stylosa increased in stem diameter and growth rate toward the riverside. 相似文献
7.
Yusuke Takata Tsuneo Kuwagata Kazunori Kohyama Hiroshi Obara 《Soil Science and Plant Nutrition》2013,59(2):294-302
The soil temperature regime map provides for utilitarian classification that can be superimposed on soil classification to permit more precise interpretations and assessments of land use. The objects of this study are (1) to clarify the relationship between soil temperature and meteo-geographical factors, and then (2) to delineate detailed soil temperature regime map (1?km grid) as Japanese land resources inventory. There was a parallel relationship between mean annual soil temperature (MAST) and mean annual air temperature (MAAT), but this relationship was affected to some extent by the mean annual wind speed and mean annual global irradiation in this study. Furthermore, the difference between MAST and MAAT [Diff(MAST–MAAT)] showed the highest correlation with elevation. The map of RK_Diff(MAST–MAAT) was computed using this meteo-geographical relationship with the regression-kriging approach, and then the map of MAST and the soil temperature regime map were delineated using the map of MAAT and the RK_Diff(MAST–MAAT). The root mean square error of this delineation procedure was 0.47°C. It was clear that the majority of the Japanese soils had “mesic” soil temperature regime, and Japanese agricultural land was mainly distributed at “mesic” area and followed by “thermic”, “frigid”, and “hyperthermic” area. For promoting this land resource inventory, the soil temperature regime map will be uploaded on “Soil Information Web Viewer (http://agrimesh.dc.affrc.go.jp/soil_db/)”, which is provided by the National Institute for Agro-Environmental Sciences. 相似文献
8.
Inhalt: Ganzpräparate von Samenkanälchen aus Hodenbiopsien, in toto mit Hämatoxylin gefärbt und montiert, wurden mit den üblichen Ganzpräparaten isolierter Kanälchen aus exstirpierten Hoden am Modell des Ziegenbocks verglichen, um die klinische Anwendung dieser Präparationstechnik zu überprüfen. Weiterhin wurden in die Untersuchung Halbpräparate einbezogen, bei denen die isolierten Kanälchen longitudinal entzweigeschnitten und mit PAS-Hämatoxylin gefärbt wurden. In den Präparaten wurden die Anzahl der B-Spermatogonien und dey primären Spermatocyten pro Flächeneinheit der Kanälchenwand ermittelt. Statistisch wurde festgestellt, daβ es signifikante Unterschiede in der Zellzahl durch die Präparation gibt. Im Verhältnis zwischen den obengenannten Zellen war bei den verschiedenen Präparationen kein signifikanter Unterschied nachweisbar. Dies weist darauf hin, daβ die Kombination der Hodenbiopsie mit der Ganzpräparationstechnik auch für klinische Untersuchungen eingesetzt werden kann. Das Halbpräparat ist wegen der mühevollen Technik in erster Linie für wissenschaftliche Fragestellungen zu empfehlen. Contents: Usefulness of whole and half mount preparations of seminiferous tubules isolated from testicular biopsy specimens in male goats With the intention of examining clinical usefulness of whole mounts of seminiferous tubules, a comparison was made between the seminiferous tubules isolated from testicular biopsy materials, stained and mounted in toto, and the tubules prepared routinely from removed testes. Another experiment was carried out to prepare half mounts of tubules, in which isolated tubules were longitudinally bisected and stained with PAS-hematoxylin technique. The numbers of type B spermatogonia and primary spermatocytes per unit surface of the tubule were counted using these various tyres of preparations. It was statistically proved that there are significant differences in the cell numbers among the preparations. As for the ratio between the numbers of the cells mentioned above, however, there are no significant differences among the preparations. Therefore, the whole amount preparation combined with testicular biopsy will have a clinical use. But the half tubular mounts will be of little clinical use because of its laborious techniques. 相似文献
9.
Rapid multiple immunofluorescent staining for the simultaneous detection of cytokeratin and vimentin in the cytology of canine tumors 
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The firmness of whole beans and of separated embryos and testas from 20 soybean cultivars grown in the same field conditions was estimated after 40 min cooking with AACC International Approved Method 56‐36.01. There were considerable variations in the firmness of whole beans, embryos, and testas among cultivars. Testas were much firmer than embryos because of their leathery texture and resistance at slots in the shear cell during compression. For soft cultivars, the firmness after 10 min cooking was also measured, and an apparent first‐order reaction kinetics model was applied to estimate the initial firmness at 0 min cooking and the softening rate constant from firmness data after 10 and 40 min cooking. The estimated initial firmness at 0 min cooking and softening rate constant of embryos and testas varied widely among seven cultivars, and these two parameters were considered to be controlled by different genetic mechanisms, because no correlation was found between the parameters. The softening rate constants of testas were much smaller than those of embryos, and the relative firmness of testas to embryos increased during cooking. The firmness of whole beans and embryos after 10 min cooking would be a useful characteristic for approximating their firmness after 40 min cooking. 相似文献