Experimental trials on the use of radioimmunoassay for the detection of leptospiral antigens in urine   总被引：1，自引：0，他引：1  

A R Bahaman  R B Marshall  K M Moriarty 《Veterinary microbiology》1986,12(2):161-167

Trials were conducted on the use of the solid phase radioimmunoassay (RIA) to detect leptospires or their antigens in simulated urine samples. The procedure was relatively simple to perform and appeared to be specific in detecting certain numbers of leptospiral organisms or their antigens in experimentally prepared samples. With this technique, it was possible to examine individual or pooled urine samples for the presence of leptospires within half a day. This technique may be of value for the detection of leptospiruric animals if the sensitivity of the technique could be further increased. Suggestions for the improvement of the procedure are discussed.  相似文献   

Continuing education in immunology. I: the structure and function of immunoglobulins     

Moriarty KM 《New Zealand veterinary journal》1984,32(5):61-64

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Epidermal Langerhans cells in the opossum, Trichosurus vulpecula     

Moriarty KM  Thomas MJ 《New Zealand veterinary journal》1986,34(1-2):13-14

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Production of Lambs After the Transfer of Fresh and Cryopreserved in vitro Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex-sorted Frozen-thawed Spermatozoa     

KM Morton  SL Catt  FK Hollinshead  WMC Maxwell  G Evans 《Reproduction in domestic animals》2004,39(6):454-461

Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm.  相似文献   

Two-dimensional nanosheets produced by liquid exfoliation of layered materials   总被引：1，自引：0，他引：1  

Coleman JN  Lotya M  O'Neill A  Bergin SD  King PJ  Khan U  Young K  Gaucher A  De S  Smith RJ  Shvets IV  Arora SK  Stanton G  Kim HY  Lee K  Kim GT  Duesberg GS  Hallam T  Boland JJ  Wang JJ  Donegan JF  Grunlan JC  Moriarty G  Shmeliov A  Nicholls RJ  Perkins JM  Grieveson EM  Theuwissen K  McComb DW  Nellist PD  Nicolosi V 《Science (New York, N.Y.)》2011,331(6017):568-571

If they could be easily exfoliated, layered materials would become a diverse source of two-dimensional crystals whose properties would be useful in applications ranging from electronics to energy storage. We show that layered compounds such as MoS(2), WS(2), MoSe(2), MoTe(2), TaSe(2), NbSe(2), NiTe(2), BN, and Bi(2)Te(3) can be efficiently dispersed in common solvents and can be deposited as individual flakes or formed into films. Electron microscopy strongly suggests that the material is exfoliated into individual layers. By blending this material with suspensions of other nanomaterials or polymer solutions, we can prepare hybrid dispersions or composites, which can be cast into films. We show that WS(2) and MoS(2) effectively reinforce polymers, whereas WS(2)/carbon nanotube hybrid films have high conductivity, leading to promising thermoelectric properties.  相似文献   

Device Applications of Side-Chain Ferroelectric Liquid Crystalline Polymer Films     

KM Blackwood 《Science (New York, N.Y.)》1996,273(5277):909-912

Side-chain ferroelectric liquid crystalline polymers are currently used in a number of applications, including displays and electrical sensors. Comparisons with existing technologies and materials indicate that relative to ceramics, such polymers have lower figures of merit but offer greater durability in sensor applications.  相似文献   

Antibodies to peptides detect new hepatitis B antigen: serological correlation with hepatocellular carcinoma   总被引：26，自引：0，他引：26  

A M Moriarty  H Alexander  R A Lerner  G B Thornton 《Science (New York, N.Y.)》1985,227(4685):429-433

The expression of a previously unidentified gene product, encoded by the hepatitis B virus (HBV) genome, has been achieved with a recombinant SV40 expression vector. Antibodies against synthetic peptides representing defined regions of this protein were used to screen cells infected with recombinant virus as well as tissues naturally infected with HBV. A 24,000-dalton protein (p24) was detected in cells infected with recombinant virus and a 28,000-dalton protein (p28) was detected in tissues infected with HBV. The peptides or recombinant-derived protein were used as antigens to screen sera from individuals infected with HBV. Specific antibodies were detected predominantly in sera from patients with hepatocellular carcinoma. The presence of p28 in tissues infected with HBV and the appearance of specific antibodies in infectious sera establish the existence of an additional marker for HBV infection.  相似文献   

Possible increase in catch of Atlantic elver Anguilla anguilla in 1993 and 1994     

C. Moriarty  F.-W. Tesch 《Ecology of Freshwater Fish》1996,5(4):213-215

Data on catches of O+ elvers from nine countries in Europe and of juvenile eels ascending the St. Lawrence River in Canada are presented and compared with previous records. Consideration of a parallel decline in recruitment in Canada supports the theory that oceanic conditions are responsible for the observed fall in catches. A substantial increase in the Loire, to 95 tons in 1994, may indicate an overall improvement in stocks.  相似文献   

Impact of Distillery Effluent on Soil Carbon and Nitrogen Dynamics in a Vertisol of Central India     

AK Biswas  Pankaj Sharma  KM Hati  S Ramana  AB Singh 《Communications in Soil Science and Plant Analysis》2018,49(17):2189-2196

Distilleries produce a huge quantity of effluents, popularly known as spent wash (SW), which when bio-methanated produce post-methanation effluents (PME). A field experiment on soybean–wheat system was conducted for five consecutive years in a Vertisol of central India to evaluate the effect of distillery effluent (DE) on soil carbon and nitrogen dynamics. Ten treatment combinations consisting of control, 100% NPK + Farmyard Manure (FYM), and graded level of SW and PME were applied. Total carbon content of soil increased significantly with applications of FYM and DE. SW was found superior in enhancing carbon content of soil in comparison to PME. Farmyard Manure contributed more carbon toward the recalcitrant pool, whereas DE contributed more carbon toward the active and slow pool. Nitrogen (N) availability was significantly improved with the application of DE. Balanced application of DE may act as amendment for increasing C and N stocks in Vertisol.  相似文献   

Classical swine fever in Victorian domestic pigs: evidence of disease freedom     

JC Hunnam  KM Moore  P Daniel  MA Stevenson  SE Salmon 《Australian veterinary journal》2019,97(11):447-451

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