Guidelines for euthanasia of domestic animals by firearms   总被引：2，自引：2，他引：0       下载免费PDF全文

Longair JA  Finley GG  Laniel MA  Mackay C  Mould K  Olfert ED  Rowsell H  Preston A 《The Canadian veterinary journal. La revue veterinaire canadienne》1991,32(12):724-726

All animals that are to be killed, whether for food, for humane reasons, or because they are homeless, must receive a quick and painless death. In some smaller communities, veterinary or humane society expertise may not be readily available to humanely kill stray and unwanted animals. An alternative that provides for a humane death for the animal is by shooting. The following guidelines are intended to assist persons who must perform this usually distasteful task; they contain recommended techniques that will help to ensure that any animals killed by shooting will die in a humane way.  相似文献   

Influence of fibrolytic enzymes on the hydrolysis and fermentation of pure cellulose and xylan by mixed ruminal microorganisms in vitro   总被引：3，自引：0，他引：3  

Colombatto D  Mould FL  Bhatt MK  Morgavi DP  Beauchemin KA  Owen E 《Journal of animal science》2003,81(4):1040-1050

A series of in vitro studies was conducted to determine the effects of adding a commercial enzyme product on the hydrolysis and fermentation of cellulose, xylan, and a mixture (1:1 wt/wt) of both. The enzyme product (Liquicell 2500, Specialty Enzymes and Biochemicals, Fresno, CA) was derived from Trichoderma reesei and contained mainly xylanase and cellulase activities. Addition of enzyme (0.5, 2.55 and 5.1 microL/g of DM) in the absence of ruminal fluid increased (P < 0.001) the release of reducing sugars from xylan and the mixture after 20 h of incubation at 20 degrees C. Incubations with ruminal fluid showed that enzyme (0.5 and 2.55 microL/g of DM) increased (P < 0.05) the initial (up to 6 h) xylanase, endoglucanase, and beta-D-glucosidase activities in the liquid fraction by an average of 85%. Xylanase and endoglucanase activities in the solid fraction also were increased (P < 0.05) by enzyme addition, indicating an increase in fibrolytic activity due to ruminal microbes. Gas production over 96 h of incubation was determined using a gas pressure measurement technique. Incremental levels of enzyme increased (P < 0.05) the rate of gas production of all substrates, suggesting that fermentation of cellulose and xylan was enzyme-limited. However, adding the enzyme at levels higher than 2.55 microL/g of DM failed to further increase the rate of gas production, indicating that the maximal level of stimulation was already achieved at lower enzyme concentrations. It was concluded that enzymes enhanced the fermentation of cellulose and xylan by a combination of pre- and postincubation effects (i.e., an increase in the release of reducing sugars during the pretreatment phase and an increase in the hydrolytic activity of the liquid and solid fractions of the ruminal fluid), which was reflected in a higher rate of fermentation.  相似文献   

The veterinarian and animal welfare          下载免费PDF全文

Ken L. Mould 《The Canadian veterinary journal. La revue veterinaire canadienne》1994,35(7):411-412

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Serum protein changes in grass sickness     

P Johnson  A M Dawson  D L Mould 《Research in veterinary science》1983,35(2):165-170

Polyacrylamide gel electrophoresis was used to compare serum taken from ponies before and during clinical illness confirmed as grass sickness. A consistent rise in the level of haptoglobin was seen in serum from animals which had shown symptoms for more than two days. Serum albumin was also shown to have altered mobility at the onset of clinical disease. Estimation of the haemoglobin-binding capacity confirmed the haptoglobin increase. This haptoglobin has been purified and some of its properties determined. In contrast to the situation in acute inflammatory conditions no other acute-phase proteins increased to a significant extent in grass sickness. It is concluded that the neurotoxin known to be present in sera of animals experiencing acute grass sickness cannot itself be detected by polyacrylamide gel electrophoresis but may be bound to serum albumin.  相似文献   

玉米F_2群体中偏分离RFLP标记在染色体上的分布及原因分析     

曹永国  王国英  王守才  魏艳玲  卢江  谢友菊  戴景瑞 《中国农业大学学报》1998,(Z3)

研究了利用玉米85个 DNA 限制性片段长度多态性(RFLP)标记在玉米自交系7922与5003杂交 F2群体中的分离。结果表明,8.33%的标记显著或极显著地偏离了预期的孟德尔比例而表现偏分离,发现了2个偏分离的染色体热点,即第1染色体上的 umc128-umc107和第3染色体上的 asg24-asg48,其偏分离形成的主要原因是存在配子体选择。  相似文献   

The effect of cultivar on the rate and extent of combining pea straw degradability examined in vitro using the Reading Pressure Technique     

F. L. Mould  G. Hervás  E. Owen  T. R. Wheeler  N. O. Smith  & R. J. Summerfield 《Grass and Forage Science》2001,56(4):374-382

Although about 225 000 t dry matter (DM) pea straw is produced annually in the UK, no recent data exist with respect to its nutritive value when offered to ruminants. This study, to examine field pea crop waste (straw) from 12 commercial cultivars of combining peas, was therefore undertaken when material grown as part of a European field pea network trial at Reading in 1998 was made available. The straw was subjected to chemical analysis and examined in vitro using the Reading Pressure Technique to generate gas production profiles and to determine both rate and extent of DM and organic matter degradation. Ash and crude protein (CP) concentrations (69 and 65 g kg^–1 DM) were generally lower than published values for legume straws but higher, especially CP, than cereal straws. Neutral‐detergent fibre concentrations were slightly lower, and metabolizable energy (ME) values, derived from degradable organic matter assessed in vitro (iOMD), higher than cereal straws, ranging from 6·0 to 8·1 MJ ME kg^–1 DM. Both cumulative gas production and rate of gas release varied significantly between cultivars, as did degradation with 96‐h iOMD values ranging from 468 to 602 g kg^–1. By combining these with the quantity of material harvested, ME yields for the different cultivars were found to range from 15 to 27 GJ ME ha^–1. Considerable, and general highly significant, variation was found between the cultivars examined, which may be used to rank straws with respect to their nutritive value. The cultivars Baccara and Solara followed by Athos and Vital were readily ranked higher than the other cultivars in terms of in vitro fermentation parameters and ME yields. These data suggest that pea straw, although often incorporated into the soil in post‐harvest operations, shows excellent potential as a ruminant forage feedstuff.  相似文献   

Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs     

Robson RV Alves  Tatiana Soares  Elinaldo FL Bento  Ricardo S Roldan‐Filho  Brbara SS Souza  Marcele KN Lima  Jssica S Nascimento  Luana CBB Coelho  Roberto A S  Thmarah A Lima  Gabriel GA Gonalves  Fbio A Brayner  Luiz C Alves  Daniela MAF Navarro  Thiago H Napoleo  Patrícia MG Paiva 《Pest management science》2020,76(2):730-736

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Optimal management of on-farm resources in small-scale dairy systems of Central Mexico: model development and evaluation     

Octavio Alonso Castelán-Ortega  Carlos Galdino Martínez-García  Fergus L. Mould  Peter Dorward  Tahir Rehman  Adolfo Armando Rayas-Amor 《Tropical animal health and production》2016,48(5):951-958

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Protein electrophoretic pattern of Pasteurella haemolytica.     

D A Thompson  D L Mould 《Research in veterinary science》1975,18(3):342-343

Electrophoresis in polyacrylamide gels of the constituent proteins of the 12 serotypes and an untypable strain of Pasteurella haemolytica showed a pattern of bands that divided the group into two. This division conformed to the A and T biotype groupings of Smith (1959) although the serotype A9 showed only minor band difference from the three T serotypes 3, 4 and 10. It was not possible by this method to separate all the type strains from each other by the specific recognition of the patterns of protein mobilities produced.  相似文献   

An investigation into the bacterial contamination of goniolenses in use in clinical practice          下载免费PDF全文

Rachael Grundon  Emma Scurrell  John Mould  Emma Hayton‐Lee  Christine Heinrich 《Veterinary ophthalmology》2018,21(5):459-463

Objectives To report the incidence and evaluate the clinical significance of goniolens bacterial contamination in clinical use in dogs with three different usage protocols and one with an added cleaning protocol. Animals Studied and Methods Three groups of twenty dogs undergoing gonioscopy at a private practice in the UK had the goniolenses swabbed for bacteriology culture and identification prior to placement on the cornea. Three protocols of lens use, with 2 different types of goniolens, were studied. One protocol was then repeated with 21 dogs with a lens cleaning protocol prior to storage. Results Low levels of bacterial contamination were found in all 3 initial groups (10‐15%). No correlation was found between usage protocol used and rate of contamination and no correlation was found between length of storage between use and contamination. All bacteria cultured were considered naturally occurring commensals for the canine eye and environment. The group with a cleaning protocol had a 4.7% contamination rate. This was not statistically different from the non‐cleaning groups. Conclusions The rate of bacterial contamination of goniolenses in clinical practice is low and the bacterial contaminants consist of commensal bacteria, unlikely to be of detriment to the eye. Minimal contamination of the goniolenses was found and this did not appear to be of clinical significance. The introduction of a simple cleaning protocol did not produce a statistically significant reduction in bacterial contamination.  相似文献