Liver biopsy techniques for adult horses and neonatal foals to assess copper status   总被引：1，自引：0，他引：1  

SG PEARCE  EC FIRTH  ND GRACE  PF FENNESSY 《Australian veterinary journal》1997,75(3):194-198

Objective To evaluate standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals, as techniques for obtaining tissue for assessment of copper status. The techniques were evaluated with respect to ease of use and effect on the animal.
Procedure Twenty of 24 Thoroughbred mares and 21 of their foals were biopsied. The animals were part of a larger study of the effect of copper supplementation on copper status and the prevalence of developmental orthopaedic disease. Livers were also collected from unrelated horses and sampled to investigate the variability in the distribution of copper in liver tissue.
Result The biopsy technique caused no lasting effect on the mares, but there was an increased risk of viscus penetration associated with taking multiple biopsy cores. The use of ultrasonography to scan the target area for the liver identified four cases that were not appropriate candidates for liver biopsy, because of large intestine being located in the biopsy area. In the foals there were no serious postoperative adverse effects, nor was there any evidence of problems caused by the procedure when the abdomen was examined post-mortem at 5 months of age. In livers collected to investigate the variability of copper concentration, copper appeared to be relatively evenly distributed through the liver.
Conclusion Standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals are convenient procedures for obtaining liver tissue for assessing copper status in horses. The use of ultrasound to identify liver tissue is recommended, especially in older mares.  相似文献   

Induced maturation and spawning of domestic and wild striped bass, Morone saxatilis (Walbaum), broodstock with implanted GnRH analogue and injected hCG     

R. G. HODSON  C. V. SULLIVAN 《Aquaculture Research》1993,24(3):389-398

Abstract. A domestic striped bass. Morone saxatilis (Walbaum), broodstock was established by rearing fish to sexual maturity in ponds. A method was developed to reproduce the domestic females, and also wild females too immature to be successfully induced to spawn with injected human chorionic gonadotropin (hCG). The fish were implanted with pellets containing 100–150μg of a synthetic analogue of mammalian gonadotropin reieasing-hormone, [D-Ala⁶- Pro⁹-NEt]-LHRH (mGnRHa), in a matrix of cholesterol (CH) and cellulose. They were implanted with one fast hormone-release (80% CH) pellet and one slow hormone-release (95% CH) pellet and allowed to mature for 1–3 days, until they entered the process of final oocyte maturation and were induced to ovulate or spawn with an hCG injection. The secondary hCG injection was found to be necessary to speed the maturation of the wild fish; they otherwise would succumb to the stresses of capture, handling and confinement before they could be spawned. The total mGnRHa dosages used ranged from 33 to 111μg mGnRHa/kg body weight, and the hCG doses were either 165 or 330 IU hCG/kg body weight. Using the combined mGnRHa implant-hCG injection technique, fry production rates were comparable to those obtained using fully mature wild females taken directly from their spawning grounds.  相似文献   

Diaphragmatic rupture and biliary tract damage in a dog     

C. L. DONALD  R. LEE  M. SULLIVAN 《The Journal of small animal practice》1985,26(2):61-66

A 9-month-old bitch was presented with respiratory distress 7 days after a road traffic accident. Radiographs showed a severe hydrothorax and moderate ascites. The removal of bile-stained fluid by thoracentesis reduced the dyspnoea. A diaphragmatic and a hepatic duct rupture were found and repaired at laparotomy. A biliary peritonitis and pleuritis was present. A blood bilirubin of 67 μmol/1 and elevated serum enzymes were present prior to surgery. These changes were largely reversed once the leakage was stopped.  相似文献   

First case of psittacine proventricular dilatation syndrome (macaw wasting disease) in Australia     

ND SULLIVAN  JT MACKIE  RI MILLER  A. GILES 《Australian veterinary journal》1997,75(9):674-674

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Endoscopy (fibreoptic) of the oesophagus and stomach in the dog with persistent regurgitation or vomiting   总被引：1，自引：0，他引：1  

M. SULLIVAN  A. MILLER 《The Journal of small animal practice》1985,26(7):369-379

A flexible gastrointestinal panendoscope was used to examine 90 dogs; 29 for oesophageal and 61 for gastric investigation. Twenty-two of the oesophageal cases had changes associated with foreign body obstruction. The commonest feature was simple mucosal damage. Small numbers of strictures (5), diverticula (1) and perforations (4) were identified. The remainder of the cases were vascular rings (5) and leiomyomata (1). The 61 gastroscopies could be subdivided into the following groups: 1, no abnormalities (14); 2, chronic gastritis (16); 3, neoplasia (26); 4, others (5). Further, gastric carcinoma accounted for 36 per cent of all gastroscopic examinations. Oesophageal abnormalities were found in 13 per cent of those cases investigated for, and found to have, gastric lesions, where reflux oesophagitis (5) was the commonest lesion.  相似文献   

Multi-orientation thin sectioning for the determination of 3-D morphology     

L. A. SULLIVAN  A. J. KOPPI 《European Journal of Soil Science》1987,38(2):383-385

A new soil thin sectioning technique which gives thin cross-sections (called sliver sections) of thin sections, is described. Optical analysis of soil thin sections and soil sliver sections, using plain, crossed polarized and circularly polarized light provides three-dimensional information on the micromorphology of clay coatings. This new technique is applied to a common type of clay plug and shows that these features consist of a stack of bowl-shaped clay coatings.  相似文献   

Teratogenic effect of 5-hydroxytryptamine in mice     

POULSON E  ROBSON JM  SULLIVAN FM 《Science (New York, N.Y.)》1963,141(3582):717-718

The subcutaneous injection of a single dose of 5-hydroxytryptamine into pregnant mice produced a large number of fetal abnormalities, mostly of the eyes, limbs, and tail; the skull and central nervous system were also sometimes affected. These effects could result from the action of the drug on placental function and blood supply.  相似文献   

MAGNETIC RESONANCE IMAGING FEATURES OF DISCOSPONDYLITIS IN DOGS     

INÉS CARRERA  MARTIN SULLIVAN  FRASER MCCONNELL  RITA GONÇALVES 《Veterinary radiology & ultrasound》2011,52(2):125-131

The diagnosis of discospondylitis is based mainly on diagnostic imaging and laboratory results. Herein, we describe the magnetic resonance imaging (MRI) findings in 13 dogs with confirmed discospondylitis. In total there were 17 sites of discospondylitis. Eleven (81.1%) of the dogs had spinal pain for >3 weeks and a variable degree of neurologic signs. Two dogs had spinal pain and ataxia for 4 days. Radiographs were available in nine of the dogs. In MR images there was always involvement of two adjacent vertebral endplates and the associated disk. The involved endplates and adjacent marrow were T1‐hypointense with hyperintensity in short tau inversion recovery (STIR) images in all dogs, and all dogs also had contrast enhancement of endplates and paravertebral tissues. The intervertebral disks were hyperintense in T2W and STIR images and characterized by contrast enhancement in 15 sites (88.2%). Endplate erosion was present in 15 sites (88.2%) and was associated with T2‐hypointense bone marrow adjacent to it. In two sites (11.8%) endplate erosion was not MR images or radiographically. The vertebral bone marrow in these sites was T2‐hyperintense. Epidural extension was conspicuous in postcontrast images at 15 sites (88.2%). Spinal cord compression was present at 15 sites (88.2%), and all affected dogs had neurologic signs. Subluxation was present in two sites (11.8%). MRI shows characteristic features of discospondylitis, and it allows the recognition of the exact location and extension (to the epidural space and paravertebral soft tissues) of the infection. Furthermore, MRI increases lesion conspicuity in early discospondylitis that may not be visualized by radiography.  相似文献   

The radiological features of sixty cases of intra-nasal neoplasia in the dog     

M. SULLIVAN  R. LEE  C.A. SKAE 《The Journal of small animal practice》1987,28(7):575-586

The radiographs of 60 cases of canine nasal neoplasia were reviewed. The dorso-ventral intra-oral view provided most information and the lateral view supplied supplementary detail. The most common changes were turbinate destruction rostrally and caudally, with a homogenous increase in radiopacity. Bilateral involvement was present in 38 cases. Absence (26) and thinning (7) of the vomer bone were found, as was deviation of the cartilaginous septum (11). Destruction of the maxilla or palatine bones produced radiolucencies which were superimposed on the increased radio-opacity in 17 cases.  相似文献   

Thyroid tumours in the dog     

M. SULLIVAN  F. COX  M.J. PEAD  P. McNELL 《The Journal of small animal practice》1987,28(6):505-512

The clinical, radiological and histopathological features of 30 thyroid tumours in the dog are described. Ten tumours were considered suitable for surgical removal and in a follow-up period of between 14 and 36 months five dogs were still alive.  相似文献