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Methods for visualizing hyaline fungi for the detection of antifungal compounds on thin-layer chromatograms 总被引：1，自引：0，他引：1
Two methods facilitating the use of hyaline fungi for the detection of antifungal compounds on thin-layer chromatograms are described. Fungal growth was visualized under ultraviolet light either by the metabolic release of fluorescein from the esterase substrate fluorescein diacetate, or by the binding of a fluorescent brightener (Calcofluor White M2R New, or Tinopal-CBS) to the mycelium on the TLC plate.
These methods were used to demonstrate compounds inhibitory to the wood-decay fungus Heterobasidion annosum in bark extracts of Sitka spruce ( Picea sitchensis ). 相似文献
These methods were used to demonstrate compounds inhibitory to the wood-decay fungus Heterobasidion annosum in bark extracts of Sitka spruce ( Picea sitchensis ). 相似文献
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Objective To evaluate standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals, as techniques for obtaining tissue for assessment of copper status. The techniques were evaluated with respect to ease of use and effect on the animal.
Procedure Twenty of 24 Thoroughbred mares and 21 of their foals were biopsied. The animals were part of a larger study of the effect of copper supplementation on copper status and the prevalence of developmental orthopaedic disease. Livers were also collected from unrelated horses and sampled to investigate the variability in the distribution of copper in liver tissue.
Result The biopsy technique caused no lasting effect on the mares, but there was an increased risk of viscus penetration associated with taking multiple biopsy cores. The use of ultrasonography to scan the target area for the liver identified four cases that were not appropriate candidates for liver biopsy, because of large intestine being located in the biopsy area. In the foals there were no serious postoperative adverse effects, nor was there any evidence of problems caused by the procedure when the abdomen was examined post-mortem at 5 months of age. In livers collected to investigate the variability of copper concentration, copper appeared to be relatively evenly distributed through the liver.
Conclusion Standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals are convenient procedures for obtaining liver tissue for assessing copper status in horses. The use of ultrasound to identify liver tissue is recommended, especially in older mares. 相似文献
Procedure Twenty of 24 Thoroughbred mares and 21 of their foals were biopsied. The animals were part of a larger study of the effect of copper supplementation on copper status and the prevalence of developmental orthopaedic disease. Livers were also collected from unrelated horses and sampled to investigate the variability in the distribution of copper in liver tissue.
Result The biopsy technique caused no lasting effect on the mares, but there was an increased risk of viscus penetration associated with taking multiple biopsy cores. The use of ultrasonography to scan the target area for the liver identified four cases that were not appropriate candidates for liver biopsy, because of large intestine being located in the biopsy area. In the foals there were no serious postoperative adverse effects, nor was there any evidence of problems caused by the procedure when the abdomen was examined post-mortem at 5 months of age. In livers collected to investigate the variability of copper concentration, copper appeared to be relatively evenly distributed through the liver.
Conclusion Standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals are convenient procedures for obtaining liver tissue for assessing copper status in horses. The use of ultrasound to identify liver tissue is recommended, especially in older mares. 相似文献
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G. A. PEARCE 《Weed Research》1963,3(3):215-229
Summary. The variation in the percentage of dormant corms of Homeria breyniana between sites and years was shown for a 3-year period. The highest dormancy count of 61 % was obtained at Wagin in 1959 while the lowest count of 1% was also obtained in 1959 at Muchea. It was demonstrated that soil surface cover plays an important part in corm dormancy. In 1961 the removal of the surface cover by burning almost eliminated dormancy. Removing the cover at three dates during the summer did not affect the germination of corms during the late autumn. Moreover, corms left in the soil throughout the summer exhibited no physiological dormancy in April. In laboratory tests the percentage germination steadily decreased as the test temperatures were reduced from 25 to 5° C. Soil temperatures follow a similar decline in Western Australia during April and May. Light rains of 12 and 14 points (100 points = 1 in.) gave a significant increase in soil moisture where the surface was bare. Sufficient moisture penetrated to cause a general germination of corms and this resulted in H. breyniana emerging on bare soil some 3 weeks earlier than where surface cover was present. The diurnal temperature range at this time was 54–68° F. The break of the season (the beginning of the growing season) did not occur until 17 days later, and the diurnal soil temperature range had dropped to 49–54° F. Corms subjected to germination tests at these two temperature ranges gave a 90% and 70% sprouting respectively.
La dormance des bulbes de Homeria breyniana (L.) Lewis 相似文献
La dormance des bulbes de Homeria breyniana (L.) Lewis 相似文献
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Accelerator mass spectrometer dating of an assemblage of fibrous and leather footwear from Arnold Research Cave in central Missouri documents a long sequence of shoe construction by prehistoric Midwestern peoples, beginning perhaps as early as 8300 calendar years before the present (cal years B.P.). An earlier fibrous sandal form dates from 8325 to 7675 cal years B.P., and later fibrous or leather slip-ons span the period from 5575 to 1070 cal years B.P. The assemblage adds to a growing picture of the highly varied nature of prehistoric footwear production in the United States throughout the Holocene. 相似文献
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Transgenic technology holds considerable promise to advance understanding in biomedical and agricultural systems with some believing that one day transgenic animals may directly contribute to farming and breeding practice. Nevertheless, applications in livestock have been restricted in part by the inefficiency of the technology. The recent development of lentivirus vectors for transgene delivery may overcome some of this limitation. This presentation describes these vectors, their advantages and limitations. 相似文献