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ATSUSHI OOSHIRO SHINJI KAWANO TETSUYA TAKUSHI NORIKO OOMI MIKA TOKESHI NORIO OOSHIRO 《Weed Biology and Management》2006,6(4):241-244
In a previous study, we found that a 70% aqueous ethanol extract of the fresh aerial tissue of Geranium carolinianum L. showed antimicrobial activity against the pathogen causing soil rot of sweet potato. As the appropriate time for cultivation of sweet potato and the growing period of G. carolinianum do not overlap in Okinawa Prefecture, Japan, the fresh aerial tissue is available in order to control soil rot of sweet potato. Thus, we examined the control effect of fresh aerial tissue against soil rot of sweet potato. The various trials (a single repetition of 20 m2 ) were performed in fields that had undergone 8 years of continuous cropping of sweet potato at Okinawa Prefectural Agricultural Experiment Station, Horticultural branch. After harvest, when the disease severity was evaluated by determining the necrotic area of the storage root, the incorporation of fresh aerial tissue (5000 kg 1000 m-2 ) into the soil was considered to be highly effective, with a protective value of 75.4. This result shows that G. carolinianum could be used as a biological agent for the control of soil rot of sweet potato. 相似文献
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Kazumi KISHIDA Mitsuhiro SAKASE Kenta MINAMI Miyuki M. ARAI Reiko SYOJI Namiko KOHAMA Takayuki AKIYAMA Akio OKA Hiroshi HARAYAMA Moriyuki FUKUSHIMA 《The Journal of reproduction and development》2015,61(6):519-524
The purposes of this study were to examine the relationship between male artificial insemination (AI)
fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify
possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with
poor acrosomal conditions in Japanese Black bulls. We investigated individual differences among bulls in the
results concerning (1) acrosomal conditions of frozen-thawed spermatozoa as assessed by not merely peanut
agglutinin-lectin staining (a conventional staining technique) but also immunostaining of acrosomal
tyrosine-phosphorylated proteins (a new staining technique), (2) routine AI using frozen-thawed spermatozoa as
assessed by pregnancy diagnosis, (3) in vivo fertilization of frozen-thawed spermatozoa and
early development of fertilized eggs as assessed by superovulation/AI-embryo collection tests and (4)
in vitro fertilization of frozen-thawed spermatozoa with oocytes. The percentages of
frozen-thawed spermatozoa with normal acrosomal conditions assessed by the abovementioned staining techniques
were significantly correlated with the conception rates of routine AI, rates of transferable embryos in
superovulation/AI-embryo collection tests and in vitro fertilization rates. These results are
consistent with new suggestions that the distribution of acrosomal tyrosine-phosphorylated proteins as well as
the acrosomal morphology of frozen-thawed spermatozoa are AI fertility-associated markers that are valid for
the prediction of AI results and that low conception rates in AI using frozen-thawed spermatozoa with poor
acrosomal conditions result from reproductive dysfunctions in the processes between sperm insemination into
females and early embryo development, probably failed fertilization of frozen-thawed spermatozoa with
oocytes. 相似文献
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Komei HOTTA Takayuki WATANABE Chiho KISHIDA Yukio NAKAMURA Nobuyuki OHKUBO Takahiro MATSUBARA Shinji ADACHI Kohei YAMAUCHI 《Fisheries Science》2003,69(3):555-562
ABSTRACT: Because blood vitellogenin (Vg) has been considered a biomarker for environmental estrogens, the basal levels of Vg and 17β-estradiol (E2 ) were determined in male Japanese whiting reared under natural conditions. Serum levels of Vg and E2 were measured and gonadal development was assessed by gonadosomatic index (GSI) and histological observation in 8–10 male fish at monthly intervals throughout the annual reproductive cycle. Serum E2 was <60 pg/mL throughout the study period. In contrast, serum Vg exhibited seasonal changes: serum levels of Vg gradually increased from April to May (mean 63 ± 13 ng/mL and 124 ± 48 ng/mL in April and May, respectively), and then reached a peak value (mean 352 ± 68 ng/mL) in June. Thereafter, serum Vg gradually decreased, reaching undetectable levels (<50 ng/mL) in October. Serum levels of Vg tended to increase in the male fish in which the GSI was >1%. Histological observation revealed that testes in such male fish were in active spermatogenesis and then all of the testes of male fish in which serum Vg decreased to ND levels were regressed. These results suggest that Vg productive potency (sensitivity to estrogens) may increase in the spermatogenic stage, resulting in production of Vg in response to very low levels of natural or xenobiotic estrogens. 相似文献
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YOSHIAKI HIKASA dvm PhD NORIKO KOJIMA dvm KATSUAKI TAKASE dvm PhD SHIGEO OGASAWARA dvm PhD 《Veterinary surgery : VS》1993,22(4):318-325
The effects of thiopental, ketamine, diazepam, xylazine and nitrous oxide, and combinations of thiopental-nitrous oxide and ketamine-nitrous oxide on electroencephalographic (EEG) spike activity and convulsive behaviors in atropinized cats at surgical depth of enflurane anesthesia were assessed quantitatively for 60 minutes during spontaneous ventilation. Mean inspired enflurane concentrations (MIEC) were reduced 16% to 29% by pretreatment with thiopental, ketamine, diazepam, and xylazine, and were reduced 19% by 66% nitrous oxide. The MIEC of cats anesthetized with thiopental-nitrous oxide-enflurane and ketamine-nitrous oxide-enflurane were 35% to 38% lower than that with nitrous oxide-enflurane. Pretreatment with thiopental, ketamine, diazepam, and xylazine did not reduce the EEG spike frequency during anesthesia but did markedly reduce the spike amplitude. The addition of 66% nitrous oxide did not alter the spike frequency during anesthesia but tended to reduce the spike amplitude. Combinations of thiopental-nitrous oxide and ketamine-nitrous oxide almost abolished the spike activity. The addition of 66% nitrous oxide prevented convulsive responses elicited by photic and auditory stimulation during enflurane anesthesia. Treatment with thiopental, ketamine, diazepam and xylazine, and combinations of thiopental-nitrous oxide and ketamine-nitrous oxide, completely prevented convulsive responses during enflurane anesthesia. 相似文献
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新城疫病毒某水禽分离株经鸡体传代后由非致病型转变为速发型的研究 总被引：8，自引：0，他引：8
于圣青 丁铲 NORIKO KISHIDA HIROSHI ITO HIROSHI KIDA KOICHI OTSUKI TOSHIHIRO ITO 《中国预防兽医学报》2003,25(1):59-64
最近，一株无毒力的新城疫病毒（NDV）在鸡体内繁殖时，变成了强毒株，但致今尚未能证实，经鸡体传代的野生水禽新城疫病毒是否也具有变成速发型毒株的能力，为了通过实验证明从水禽中分离的非致病型NDV可以转变为速发型病毒，我们通过在鸡体内传播鹅源性无毒力株，经气囊接种连续传代9次，随后再在鸡脑内传代5次，结果显示，该病毒的毒力变得很强，致死率可达100%，通过致病性试验证实，其具有典型的速发型病毒特征；融合蛋白裂解位点的序列分析表明，原始的分离株含有无毒力型毒株共有的裂解序列；E-R-Q-E-R/L，而通过鸡体反复传代后，该序列变为致癞 性毒株共有的序列：K-R-Q-K-R/F，这些结果表明，野生水禽中自然存在的无毒力毒株，具有无毒力株相应的序列，但当其在鸡群中传播时，则具有变成高致病性病毒的能力，同时研究表明，鸡体提供了该病毒从非致病型向致病型转变的选择机制。 相似文献
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Tetsuya SHIMODA Mika TANABE Yuuki SHOJI Takuya KANDA Kouhei KISHIDA Ai KISHIDA Atsushi HASHIMOTO Mako OTSUKA Akina MIYAMOTO Tomoko KAWAKITA Wataru OSHITA Reijiro HIRAO Akihisa SUWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):251
A cat was presented with depression and anorexia. The complete blood cell count (CBC) revealed non-regenerative anemia (PCV, 8.5%), marked thrombocytopenia (2,400/µl), and leukocytosis (32,090/µl). In the peripheral blood, proliferation of blast cells (85%; 27,276/µl) and basophils (7.7%; 2,460/µl) was observed. Bone marrow aspirate showed hyperplasia with 8.8% blasts and 90.2% basophils of all nucleated cells. The blast cells were negative for myeloperoxidase staining and positive for alpha-naphthol butyrate esterase staining, indicating the agranular blasts are monoblasts. Thus, acute monoblastic leukemia (M5a) with chronic basophilic leukemia was diagnosed. Basophils accounted for more than 40% of the bone marrow, and we diagnosed secondary basophilic leukemia. Secondary basophilic leukemia should be included in the differential list when abnormal basophil increases are observed in feline bone marrow. 相似文献
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