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1.
Chhaya P. Sonekar Satyajit Kale Smita Bhoyar Neha Paliwal S. V. Shinde S. P. Awandkar Wiqar Khan S. P. Chaudhari N. V. Kurkure 《Tropical animal health and production》2018,50(1):91-96
Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss. 相似文献
2.
Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers 
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下载免费PDF全文 Kapil K. Tiwari Anshuman Singh Sasmita Pattnaik Maninder Sandhu Sukhdeep Kaur Sourabh Jain Sushma Tiwari Shweta Mehrotra Mahender Anumalla Rashmita Samal Jyoti Bhardwaj Neha Dubey Vikrant Sahu Gayle A. Kharshing Patu K. Zeliang Kadiri Sreenivasan Pankaj Kumar Swarup K. Parida Sevanthi V. A. Mithra Vandana Rai Wricha Tyagi Pawan K. Agrawal Atmakuri R. Rao Arunava Pattanayak Girish Chandel Ashok K. Singh Ishwari S. Bisht Kangila V. Bhat Gundimeda J. N. Rao Jitendra P. Khurana Nagendra K. Singh Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171
Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining. 相似文献
3.
Vinay Kumar Midha V. K. Kothari R. Chattopadhyay A. Mukhopadhyay 《Fibers and Polymers》2010,11(4):661-668
In this paper artificial neural network (ANN) model has been designed to predict the strength loss in threads during high
speed industrial sewing. Four different types of threads (Mercerized cotton, polyester staple spun, polyester-cotton core
spun and polyester-polyester core spun) were taken for the study. The other input parameters include thread linear density,
fabric area density, number of fabric layers, stitch density and needle size. In order to reduce the dependency of the results
on a specific partition of the data into training and testing sets, a four-way cross validation tests were performed, i.e.
total data was divided into training and testing set in four different ways. The predicted tenacity loss was correlated to
the experimental tenacity loss and correlation coefficient between the actual and predicted tenacity loss obtained. It was
observed that the neural network system is able to predict the tenacity loss of threads after sewing with good correlation
and less average error. The relative contribution of each parameter to the overall prediction of the tenacity loss was studied
by carrying out the sensitivity analysis of the test data set. The results of sensitivity analysis show that thread type is
the most important input parameter followed by thread linear density, number of fabric layers, fabric area density, needle
size and the stitch density. 相似文献
4.
Soil salinity diminishes soil health and reduces crop yield, which is becoming a major global concern. Salinity stress is one of the primary stresses, leading to several other secondary stresses that restrict plant growth and soil fertility. The major secondary stresses induced in plants under saline-alkaline conditions include osmotic stress, nutrient limitation, and ionic stress, all of which negatively impact overall plant growth. Under stressed conditions, certain beneficial soil microflora ... 相似文献
5.
When large carnivores cause socioeconomic losses in a community, conflict increases, retaliatory killing of the carnivore can occur, and conservation efforts are undermined. We focused on Participatory Rural Appraisal (PRA) and economic compensation schemes as approaches for managing conflict. PRA is a tool for collecting data on the large carnivore–human conflict and economic compensation schemes for those affected negatively by carnivore presence. We reviewed published papers and reports on large carnivore–human conflicts, PRA, and compensation schemes. This article details insights into common pitfalls, key lessons learned, possible solutions including new approaches for compensation and protocols to be followed while managing large carnivore–human conflict. We hope to contribute to a meaningful dialogue between locals, managers, and researchers and help in effective implementation of conservation programs to mitigate large carnivore–human conflict around the protected areas. 相似文献
6.
Jitendra Kumar Gautam Saripalli Vijay Gahlaut Neha Goel Prabina Kumar Meher Kaushlesh Kumar Mishra Prafulla Chandra Mishra Deepmala Sehgal Prashant Vikram Carolina Sansaloni Sukhwinder Singh Pradeep Kumar Sharma Pushpendra Kumar Gupta 《Euphytica》2018,214(11):219
The present study was conducted to study the genetic architecture of grain micronutrients (Zn, Fe and β-carotene contents), grain protein content and four yield traits in a spring wheat reference set comprising 246 genotypes. Phenotypic data on these traits recorded at two locations and the genotyping data for 17,937 SNP markers (obtained through outsourcing) were used for genome wide association study, which gave following results after Bonferroni correction using four methods: (1) single locus single trait analysis gave 136 marker-trait associations; (2) multi-locus mixed model gave 587 MTAs; (3) multi-trait mixed model gave 28 MTAs and (4) matrix-variate linear mixed model gave 33 MTAs. As many as 73 epistatic interactions were also detected. Keeping all the results in mind, nine most important MTAs were selected for biofortification. These markers were associated with three traits (GPC, GFeC and GYPP). These MTAs can be used in wheat improvement programs either using marker-assisted recurrent selection or pseudo-backcrossing method. 相似文献
7.
Acorus calamus is a highly valued medicinal plant with global distribution used in several drugs of health care systems. We evaluated the genetic diversity and population structure of 50 populations of A. calamus from different geographical regions in India through RAPD and chloroplast microsatellite markers. From the total screened 82 RAPD primers and 18 cpSSR primers, 10 RAPD and nine cpSSRs were found polymorphic. The selected 10 RAPD primers produced a total of 96 reproducible bands, out of which 65 wer... 相似文献
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9.
Marker‐assisted backcross breeding for improvement of drought tolerance in bread wheat (Triticum aestivum L. em Thell) 下载免费PDF全文
下载免费PDF全文 Neha Rai Amasiddha Bellundagi Prashant K. C. Kumar Ramya Kalasapura Thimmappa Sushma Rani Nivedita Sinha Hari krishna Neelu Jain Gyanendra P. Singh Pradeep K. Singh Suresh Chand Kumble Vinod Prabhu 《Plant Breeding》2018,137(4):514-526
Drought stress is presently a major productivity limiting factor in wheat. This study developed five wheat lines with inbuilt tolerance to drought stress using marker‐assisted backcross breeding (MABB) approach employing three linked quantitative trait loci (QTLs) in an initial population of 516 BC1F1 plants. The high‐yielding wheat cultivar ‘HD2733’ grown over last few years extensively in the eastern plains of India is largely sensitive to drought and is used as the recurrent parent. ‘HI1500’ released for water‐limiting conditions and carrying drought‐tolerant QTLs was used as donor parent. MABB lines were advanced using foreground and background selection, coupled with stringent phenotyping. We identified 29 lines that were homozygous for targeted QTLs in different combinations with background recovery range of 89.2%–95.4%. Further evaluation of selected lines for physiological traits and distinctness, uniformity and stability (DUS) characters under rainfed condition identified five potential varieties for national varietal evaluation programme in the zone. The report is first of its kind in implementing known QTLs for the development of drought‐tolerant wheat lines through MABB approach. 相似文献
10.
Chien-Yuan Lin Bryon S. Donohoe Neha Ahuja Deborah M. Garrity Rongda Qu Melvin P. Tucker Michael E. Himmel Hui Wei 《Plant methods》2017,13(1):113