Identification of Cytokeratins in Bovine Sperm Outer Dense Fibre Fractions     

E Hinsch  JG Boehm  S Groeger  F Mueller-Schloesser  K-D Hinsch 《Reproduction in domestic animals》2003,38(2):155-160

Outer dense fibres (ODF) are important substructures of mammalian sperm tails that are involved in the regulation of sperm motility. In this study, we investigated the identity of several sodium dodecyl sulphate (SDS)‐insoluble ODF proteins. Bovine ODF were purified by separating sperm heads and tails using ultrasound and Percoll^® density gradient centrifugation. Sperm flagella were treated with the detergent cetyltrimethylammonium bromide (CTAB). CTAB‐insoluble material, which reportedly represents the ODF fraction, was collected, and electron microscopy confirmed a highly purified ODF fraction. We found after solubilization of this fraction with SDS that high amounts of insoluble material were retained after centrifugation. SDS‐insoluble material was collected and quantitatively dissolved in 8 M urea. SDS‐gel electrophoresis in the presence of urea revealed polypeptides with apparent molecular masses of approximately 25, 43, and 50 kDa. Subsequent immunoblotting with anti‐cytokeratin antibodies detected two urea‐soluble, SDS‐insoluble proteins with apparent molecular masses of 45 and 66 kDa. The 45‐kDa protein was identified as cytokeratin 19. An antibody reacting with a palette of cytokeratins (CK 1–18 and CK 20), KL1, was the only antibody that reacted with the 66‐kDa polypeptide. We conclude that sperm ODF fractions contain at least one each of type I and type II intermediate filaments. As keratins and intermediate filaments are described as rope‐like structures, we suggest that these intermediate filaments play an important structural or tension‐bearing role in sperm flagella.  相似文献   

Bull Evaluation Workshop     

JG McLean 《Australian veterinary journal》1993,70(11):430-430

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Effects of Confluent, Roscovitine Treatment and Serum Starvation on the Cell-cycle Synchronization of Bovine Foetal Fibroblasts     

SR Cho  SA Ock  JG Yoo  B Mohana kumar  SY Choe  GJ Rho 《Reproduction in domestic animals》2005,40(2):171-176

The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors.  相似文献   

Evaluation of the Effects of Different Stunning Methods on the Stress Responses and Meat Quality of the Amazon hybrid surubim,Pseudoplatystoma fasciatum female × Leiarius marmoratus male     

Elayna Cristina da Silva Maciel  Edivaldo Sampaio de Almeida Filho  William Bertoloni  Janessa Sampaio de Abreu 《Journal of the World Aquaculture Society》2014,45(1):35-44

Different stunning methods (carbon dioxide [CO₂] and hypothermia) used in industrial fish processing were compared with asphyxia in air, which is used in traditional fishing, to evaluate the effects of these methods on the stress responses and the meat quality of the Amazon hybrid surubim, Pseudoplatystoma fasciatum female × Leiarius marmoratus male. After the application, blood and behavioral indicators were evaluated. Following death by gill cutting, the fish were gutted, and analyses of muscle pH, rigor mortis, the exudation of the meat, and coloration were performed. Significantly higher levels of cortisol and glucose were exhibited by the fish subjected to asphyxia than by the fish stunned by either CO₂ or hypothermia. The fish subjected to asphyxia displayed decreasing muscle pH during the first 2 h after death and showed higher rates of rigor mortis after 3 h than the fish that were stunned by the other two methods. The asphyxia is a practice that exposes fish to suffering by causing an increase in stress responses, which affects meat quality. The immersion of fish in water and ice (hypothermia) for 5 min was more effective stunning method than the use of CO₂, resulting in higher loss of sensibility and greater welfare.  相似文献   

Plasma biomarkers profile of female dogs with mammary carcinoma and its association with clinical and pathological features          下载免费PDF全文

R. P. Soares  L. G. R. Ribeiro  K. A. Damasceno  A. T. Costa  A. Teixeira‐Carvalho  O. A. Martins‐Filho  G. D. Cassali 《Veterinary and comparative oncology》2016,14(1):88-100

The immunological biomarkers profiles were evaluated using Luminex as putative measures to monitor canine mammary carcinomas (MCs). Forty female dogs were categorized into benign mixed tumour (MC‐BMT = 28) and mammary carcinoma (MC=12). The ascendant biomarker signatures were used to compare the groups. For example, a higher frequency of MC‐BMT animals producing IL‐6, CXCL‐8 and CXCL‐10 was observed, whereas for the MC group IL‐2 and CXCL‐8 were detected. MC‐BMT animals without metastasis had an increase in the levels of IL‐2, CXCL‐8, CXCL‐10, IL‐6, TNF‐α, IL‐15 and a decrease in IL‐10 and CXCL‐8. MC‐BMT animals with metastasis showed only an increase in CXCL‐10 and a decrease in IL‐18. After comparing the ascendant signatures following the presence of metastasis in both groups, a higher frequency of dogs exhibiting IL‐10 production was observed. Pearson correlation (P = 0.0273) and receiver operating characteristic (ROC) curve analysis revealed that this pattern was associated with worse outcome and lower survival rates in MC animals.  相似文献   

Energy and protein requirements of young Holstein calves in tropical condition     

João Paulo Pacheco Rodrigues  Jessika Carolina Moutinho Lima  Marcelo Messias Duarte Castro  Sebastião de Campos Valadares Filho  Mariana Magalhães Campos  Mário Luiz Chizzotti  Marcos Inácio Marcondes 《Tropical animal health and production》2016,48(7):1387-1394

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Theileria orientalis: a review     

JG Watts  MC Playford  KL Hickey 《New Zealand veterinary journal》2016,64(1):3-9

Theileria orientalis (also known historically as T. sergenti and T. buffeli) is responsible for benign or non-transforming theileriosis, and exerts its major effect through erythrocyte destruction. The life cycle of T. orientalis is essentially similar to that of other Theileria species, except that the schizonts do not induce transformation and fatal lymphoproliferation. The pathogenesis of anaemia as a result of infection is not clearly established and may be multifaceted. Clinical signs of weakness, reluctance to walk and abortion are early but non-specific indications of disease, particularly if accompanied by a history of cattle being moved. Physical examination may reveal pallor (pale eyes, vaginal mucosa), pyrexia, and elevated heart and respiratory rates. T. orientalis is an economically important parasite of cattle in New Zealand, Australia and Japan, especially where naïve animals are introduced into an endemic area or in animals under stress. Increased awareness of the risks posed by the parasite is required to enable management practices to be implemented to minimise its impact.  相似文献   

First report of Seville root-knot nematode (Meloidogyne hispanica) infecting tobacco in the Brazil     

de Ramos  Eduarda Kalena Kirsch  Pazdiora  Paulo Cesar  Dallagnol  Leandro José  de Souza  Mayara Rodrigues  de Araujo Filho  Jeronimo Vieira 《植物病害和植物保护杂志》2023,130(1):211-214

Journal of Plant Diseases and Protection - Tobacco (Nicotiana tabacum L.) is one of the major nonfood crops in the Southern of Brazil. During 2019/2020 and 2020/2021 crop seasons, Root Knot...  相似文献   

Temporal and spatial progress of the diseases caused by the crinivirus tomato chlorosis virus and the begomovirus tomato severe rugose virus in tomatoes in Brazil     

M. A. Macedo  A. K. Inoue-Nagata  T. N. Z. Silva  D. M. S. Freitas  J. A. M. Rezende  J. C. Barbosa  M. Michereff-Filho  A. R. Nascimento  A. L. Lourenção  A. Bergamin Filho 《Plant pathology》2019,68(1):72-84

Efficient management of whitefly-borne diseases remains a challenge due to the lack of a comprehensive understanding of their epidemiology, particularly of the diseases tomato golden mosaic and tomato yellowing. Here, by monitoring 16 plots in four commercial fields, the temporal and spatial distribution of these two diseases were studied in tomato fields in Brazil. In the experimental plots these diseases were caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV), respectively. The incidence of each virus was similar in the plots within a field but varied greatly among fields. Plants with symptoms for both diseases were randomly distributed in three of four spatial analyses. The curves representing the progress of both diseases were similar and contained small fluctuations, indicating that the spread of both viruses was similar under field conditions. In transmission experiments of ToSRV and ToCV by Bemisia tabaci MEAM1 (former biotype B), these viruses had a similar transmission rate in single or mixed infections. It was then shown that primary and secondary spread of ToCV were not efficiently controlled by insecticide applications. Finally, in a typical monomolecular model of disease progress, simulation of the primary dissemination of ToSRV and ToCV showed that infected plants were predominantly randomly distributed. It is concluded that, although the manner of vector transmission differs between ToSRV (persistent) and ToCV (semipersistent), the main dispersal mechanisms are most probably similar for these two diseases: primary spread is the predominant mechanism, and epidemics of these diseases have been caused by several influxes of viruliferous whiteflies.  相似文献   

Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs     

Robson RV Alves  Tatiana Soares  Elinaldo FL Bento  Ricardo S Roldan‐Filho  Brbara SS Souza  Marcele KN Lima  Jssica S Nascimento  Luana CBB Coelho  Roberto A S  Thmarah A Lima  Gabriel GA Gonalves  Fbio A Brayner  Luiz C Alves  Daniela MAF Navarro  Thiago H Napoleo  Patrícia MG Paiva 《Pest management science》2020,76(2):730-736

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