菜用加工兼用栗永荆3号选育     

阮逸  李德静  叶茂宗 《浙江林业科技》2001,21(5):27-29

一般认为我国南方栗仅宜于菜用，加工极易开瓣和糊化，经过几年的筛选和加工试剂，永荆3号品种可生产B－E级标准的糖水栗子罐头。  相似文献   

小鼠H-Y单克隆抗体ELISA检测方法的建立   总被引：2，自引：0，他引：2  

杭苏琴  刘为敏  牛树理  冯紫云 《中国兽医学报》2004,24(6):547-549

以纯系 BAL B/ c雄性小鼠脾细胞腹腔注射免疫同系雌性小鼠 9次 ,获得的抗血清经雌、雄鼠脾细胞吸收后用于精子细胞毒性试验 ,测得 H- Y抗血清效价为 1/ 16 0。选取免疫应答最好的雌鼠脾细胞与 SP2 / 0骨髓瘤细胞融合 ,用精子细胞毒性方法筛选效价较高的细胞株制备腹水 ,建立优化的 EL ISA反应条件。优化后的 EL ISA反应条件为 :抗原4℃过夜 ,加 H- Y抗血清 37℃反应 12 0 m in,加 HRP- Ig G 37℃反应 30 min,加 TMB 2 5℃ 30 min。优化后的 EL ISA与常规 EL ISA比较 ,可以明显降低阴性吸光值 ,检测灵敏度从 1/ 32 0上升为 1/ 12 80  相似文献   

如何提高母猪的繁殖能力     

阮景军 《畜牧兽医杂志》2005,24(5):40-42

分析了造成母猪繁殖能力低的因素主要表现为乏情期长和产仔数低，其次是不孕，流产，产弱仔，产木乃伊胎及死胎等。而繁殖障碍性疾病和饲养管理不当是突出的问题。提出了通过疾病控制和科学饲养来提高母猪繁殖能力的措施。  相似文献   

猴头菌新菌株宁猴6号的选育初报   总被引：2，自引：0，他引：2  

阮瑞国  丁李春  陈华 《江西农业大学学报》2002,24(2):293-295

采用多孢分离技术产生多个新组合 ,经驯化筛选获得猴头新菌株宁猴 6号 ,宁猴 6号为适合用针叶林木屑物料栽培的新菌株。生物学特性表明 :猴头菌宁猴 6号菌丝生长适温 2 5～ 2 8℃ ,子实体生长适温 15～2 0℃ ,菌丝生长期短 ,36～ 4 0d长满瓶底 ,栽培袋在常温下培养 4 3～ 5 0d菌丝满袋。室内栽培生物学效率达5 6 .0 0 % ,室外栽培生物学效率达 6 1.4 0 % ,子实体中型匀称近球型 ,柄短或无柄 ,刺长 0 .9～ 2 .2cm。宁猴 6号不仅适于阔叶木屑栽培 ,最突出的是适应针叶林木屑栽培  相似文献   

沙棘叶子中黄酮的分离与鉴定   总被引：13，自引：6，他引：7  

阮栋梁  王晖  李和 《沙棘》2002,15(4):32-34

利用硅胶柱和葡聚糖聚胶柱层析，从沙棘叶子中分离并鉴定出黄酮类成分，并对其结构进行了结构鉴定。  相似文献   

沙棘叶中4，4′-二羟基-2′-甲氧基查耳酮的分离与鉴定     

阮栋梁  陶海荣李和 《沙棘》2004,17(1):24-27

利用硅胶柱和葡聚糖凝胶柱层析，从沙棘叶子中分离并鉴定出黄酮类成分，进一步分离得到4，4′-二羟基-2′-甲氧基查耳酮，并对其结构进行了结构鉴定。  相似文献   

Effects of L-arginine on the production of extracellular matrix of human mesangial cells     

MA Kun-ling  LIU Bi-cheng  YE Yin-ying  LIU Nai-feng  RUAN Xiong-zhong 《园艺学报》2002,18(5):469-472

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Effects of carvedilol,cilazapril and their combination on left ventricular remodeling after acute myocardial infarction in rats     

TANG Yi-da  YANG Yue-jin  RUAN Ying-mao  LI Yong-li  ZHOU Yan-wen  TIAN Yi  GAO Run-lin  CHEN Ji-lin  CHEN Zai-jia 《园艺学报》2002,18(9):1115-1118

AIM:To compare the effects of carvedilol, cilazapril and their combination on left ventricular remodeling(LVRM) after acute myocardial infarction(AMI) in rats. METHODS: Twenty-four hours after AMI operation, 100 surviving rats were randomly assigned to: ①AMI control(n= 25), ②AMI+carvedilol(1 mg·kg^-1 ·d^-1, n= 25)(C1), ③AMI+cilazapril(1 mg·kg^-1 ·d^-1, n= 25)(Z1), and ④ AMI+combination(n= 25) groups. Sham-operated group(n= 17) were selected randomly. After 4 weeks of therapy with the drugs gastric gavage, hemodynamic and pathological studies were performed. RESULTS: There were no significant differences in MI size among the four AMI groups(all P> 0.05) Left ventricular(LV) end diastolic pressure(LVEDP), volume(LVV), weight(LVW) and septal thickness(STh) were all higher and left ventricular pressure maximal rate of rise and fall(±d p /d t) were lower(all P< 0.01) in AMI group than sham-operated group. The LVEDP, LVV, LVW and STh were all lower and ±dp /dt were higher in Z1, C1, and combination groups than those in AMI group(P< 0.05, P< 0.01), with LVEDP and STh were more lower in the combination group than in the two monotherapy group(P< 0.05, P< 0.01), but there were no significant differences in other variables among the three therapy groups. CONCLUSION: Carvedilol, cilazapril and their combination all can prevent from LVRM after AMI in rats, improve hemodynamics and LV function, with the combination superior.  相似文献   

Relation between TNF-α,TNFR I expression and apoptosis in oral lichen planus     

SHEN Li-Jia  RUAN Ping  YIN Cao  XIE Si-ming  ZHAO Tong 《园艺学报》2004,20(9):1664-1667

AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus.  相似文献   

Changes of potassium currents in rabbit ventricle with healed myocardial infarction     

LIU Nian  NIU Hui-yan  LI Yang  ZHANG Cun-tai  ZHOU Qiang  RUAN Yan-fei  PU Jun  LU Zai-ying 《园艺学报》2004,20(12):2227-2231

AIM: To elucidate the mechanism of arrhythmia in healed myocardial infarction (HMI), and to investigate the changes of action potential duration (APD),transient outward potassium current (I_to), delayed rectifier potassium current (I_K) and inward rectifier potassium current (I_K1) of left ventricular myocytes in noninfarcted zone of HMI. METHODS: 12 rabbits were randomly assigned in two groups: HMI group (thoracotomy and ligation of the circumflex coronary); sham-operated group (thoracotomy but no conorary ligation). 3 months after operation, whole cell patch clamp technique was used to record APD, I_to, I_K and I_K1 of ventricular myocytes in non-infarcted zone. RESULTS: Membrane capacitance was larger in HMI group than that in sham-operated group. Action potential duration was lengthened significantly in HMI group and early after depolarization (EAD) appeared in HMI group. The densities of I_to, I_K,tail and I_K1 were reduced significantly in HMI group (P<0.01), from (6.72±0.42) pA/pF, (1.54±0.13) pA/pF and (25.6±2.6) pA/pF in Sham-operated group to (4.03±0.33) pA/pF, (1.14±0.11) pA/pF and (17.6±2.3) pA/pF, respectively. CONCLUSION: The reduced densities of I_to, I_K,tail and I_K1 in ventricular myocytes of non-infarcted zone in HMI are responsible for the prolongation of APD and the presentation of EAD, which play important roles in the malignant arrhythmia of HMI.  相似文献