Production of Lambs After the Transfer of Fresh and Cryopreserved in vitro Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex-sorted Frozen-thawed Spermatozoa     

KM Morton  SL Catt  FK Hollinshead  WMC Maxwell  G Evans 《Reproduction in domestic animals》2004,39(6):454-461

Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm.  相似文献   

Crop water requirements for rainfed and irrigated grain corn in China   总被引：1，自引：0，他引：1  

W.H. Terjung  H-Y. Ji  J.T. Hayes  P.A. O&#x;Rourke  P.E. Todhunter 《Agricultural Water Management》1983,6(1):43-64

A basic parametric crop water use model (WATER) that employes climatic and environmental data to calculate temporal and spatial water consumption for a variety of major corps was applied specifically for grain corn to the region of China and Korea to investigate the evapotranspiration (ET) demand on grain corn and the associated irrigation water applications necessary for optimal crop production. A network of 241 stations provided the seasonal climatic input. The climatic input consisted of data averaged over approximately a 20 year period. Among the results, highest ET under full irrigation (first harvest) occurred in the northwestern inland sections of China, whereas least ET was found for the southeast. Under rainfed conditions, the relationship became nearly inverse. In order to achieve optimum crop yields, about 1000 mm of irrigation water was needed in the northwest, contrasted with none required in the south and east of China. A sensitivity analysis was applied to determine the degree of error introduced by faulty or uncertain environmental input data.  相似文献   

Device Applications of Side-Chain Ferroelectric Liquid Crystalline Polymer Films     

KM Blackwood 《Science (New York, N.Y.)》1996,273(5277):909-912

Side-chain ferroelectric liquid crystalline polymers are currently used in a number of applications, including displays and electrical sensors. Comparisons with existing technologies and materials indicate that relative to ceramics, such polymers have lower figures of merit but offer greater durability in sensor applications.  相似文献   

Outbreak of Diarrhetic Shellfish Poisoning Associated with Mussels,British Columbia,Canada     

Marsha Taylor  Lorraine McIntyre  Mark Ritson  Jason Stone  Roni Bronson  Olga Bitzikos  Wade Rourke  Eleni Galanis  Outbreak Investigation Team 《Marine drugs》2013,11(5):1669-1676

In 2011, a Diarrhetic Shellfish Poisoning (DSP) outbreak occurred in British Columbia (BC), Canada that was associated with cooked mussel consumption. This is the first reported DSP outbreak in BC. Investigation of ill individuals, traceback of product and laboratory testing for toxins were used in this investigation. Sixty-two illnesses were reported. Public health and food safety investigation identified a common food source and harvest area. Public health and regulatory agencies took actions to recall product and notify the public. Shellfish monitoring program changes were implemented after the outbreak. Improved response and understanding of toxin production will improve management of future DSP outbreaks.  相似文献   

Alkaloids from the Sponge Stylissa carteri Present Prospective Scaffolds for the Inhibition of Human Immunodeficiency Virus 1 (HIV-1)     

Aubrie O’Rourke  Stephan Kremb  Theresa Maria Bader  Markus Helfer  Philippe Schmitt-Kopplin  William H. Gerwick  Ruth Brack-Werner  Christian R. Voolstra 《Marine drugs》2016,14(2)

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Impact of Distillery Effluent on Soil Carbon and Nitrogen Dynamics in a Vertisol of Central India     

AK Biswas  Pankaj Sharma  KM Hati  S Ramana  AB Singh 《Communications in Soil Science and Plant Analysis》2018,49(17):2189-2196

Distilleries produce a huge quantity of effluents, popularly known as spent wash (SW), which when bio-methanated produce post-methanation effluents (PME). A field experiment on soybean–wheat system was conducted for five consecutive years in a Vertisol of central India to evaluate the effect of distillery effluent (DE) on soil carbon and nitrogen dynamics. Ten treatment combinations consisting of control, 100% NPK + Farmyard Manure (FYM), and graded level of SW and PME were applied. Total carbon content of soil increased significantly with applications of FYM and DE. SW was found superior in enhancing carbon content of soil in comparison to PME. Farmyard Manure contributed more carbon toward the recalcitrant pool, whereas DE contributed more carbon toward the active and slow pool. Nitrogen (N) availability was significantly improved with the application of DE. Balanced application of DE may act as amendment for increasing C and N stocks in Vertisol.  相似文献   

Experimental oral transmission of chronic wasting disease to red deer (Cervus elaphus elaphus): Early detection and late stage distribution of protease-resistant prion protein     

Aru Balachandran  Noel P. Harrington  James Algire  Andrei Soutyrine  Terry R. Spraker  Martin Jeffrey  Lorenzo González  Katherine I. O’Rourke 《The Canadian veterinary journal. La revue veterinaire canadienne》2010,51(2):169-178

Chronic wasting disease (CWD), an important emerging prion disease of cervids, is readily transmitted by intracerebral or oral inoculation from deer-to-deer and elk-to-elk, suggesting the latter is a natural route of exposure. Studies of host range susceptibility to oral infection, particularly of those species found in habitats where CWD currently exists are imperative. This report describes the experimental transmission of CWD to red deer following oral inoculation with infectious CWD material of elk origin. At 18 to 20 months post-inoculation, mild to moderate neurological signs and weight loss were observed and animals were euthanized and tested using 3 conventional immunological assays. The data indicate that red deer are susceptible to oral challenge and that tissues currently used for CWD diagnosis show strong abnormal prion (PrP^CWD) accumulation. Widespread peripheral PrP^CWD deposition involves lymphoreticular tissues, endocrine tissues, and cardiac muscle and suggests a potential source of prion infectivity, a means of horizontal transmission and carrier state.  相似文献   

Soil solution levels of nitrate nitrogen in a potato-buckwheat rotation   总被引：1，自引：0，他引：1  

Robert V. Rourke 《American Journal of Potato Research》1985,62(1):1-8

Using excessive rates of N fertilizer on potatoes in Maine has been a common practice. Excessive N did not significantly increase potato yields above the recommended N application. The impact of N fertilization upon soil solution levels of NO₃ ^-N in the B and C soil horizons was compared at three application levels of N. High N resulted in greater levels of NO₃ ^-N in the C horizon and the strong possibility of groundwater contamination. There was no apparent change in the NO₃ ^-N content of the C horizon during the winter following buckwheat, but there was an increase of NO₃ ^-N in this horizon when fall levels beneath potatoes were compared to spring values under buckwheat. After one year of no N application soil solution NO₃ ^-N levels in the B horizon returned to amounts that were the same as those receiving no N fertilization for a decade.  相似文献   

Classical swine fever in Victorian domestic pigs: evidence of disease freedom     

JC Hunnam  KM Moore  P Daniel  MA Stevenson  SE Salmon 《Australian veterinary journal》2019,97(11):447-451

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Dachshund bitch with severe uterine adhesions and intramural uterine foreign material as an incidental ovariohysterectomy finding          下载免费PDF全文

KM Watson  NU Horadagoda  SA Piripi 《Australian veterinary journal》2016,94(1-2):24-26

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