The use of muscle relaxant in dairy practice     

Vermunt J  Visser R 《New Zealand veterinary journal》1987,35(10):177-178

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‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

Comparison of footbathing and vaccination to control ovine footrot in an experimentally infected flock          下载免费PDF全文

MB Allworth  JR Egerton 《Australian veterinary journal》2018,96(10):395-399

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Diagnostic Value of Right Pulmonary Artery Distensibility Index in Dogs with Pulmonary Hypertension: Comparison with Doppler Echocardiographic Estimates of Pulmonary Arterial Pressure          下载免费PDF全文

L.C. Visser  M.K. Im  L.R. Johnson  J.A. Stern 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(2):543-552

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Diagnosis of regional evaporation by remote sensing to support irrigation performance assessment     

W. G. M. Bastiaanssen  T. Van der Wal  T. N. M. Visser 《Irrigation and Drainage Systems》1996,10(1):1-23

The success of water management in large irrigation schemes with composites of soil, crop, wetness and micro-meteorological conditions is difficult to quantify. Performance assessment indicators, being among others a function of evaporation, are useful tools to evaluate the actual functioning of an irrigation system. The inevitable spatial variability of evaporation in large irrigation schemes makes its determination with conventional point measurements almost impossible. A new remote sensing evaporation parameterization algorithm has been tested with high resolution Landsat Thematic Mapper data for the Eastern Nile Delta, Egypt. Although the implementation of such an algorithm requires assumptions to be made, the current case study shows that these assumptions do not hamper the estimation of actual and potential evaporation at regional scale. The actual evaporation has been used to express the uniformity of crop water use which is related to the equity of irrigation water distribution. The coefficient of variance in actual evaporation between 53 differenent irrigation districts is 10% on average. The relative evaporation was considered to determine whether the crop was adequately irrigated. The relative evaporation was more than 75% for 48 out of 53 irrigation districts. It is concluded, that improved information on actual crop growth conditions through remote sensing provides an essential insight into the planning of real-time and seasonal irrigation water deliveries.  相似文献   

Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations     

Antoine LF Gady  Freddy WK Hermans  Marion HBJ Van de Wal  Eibertus N van Loo  Richard GF Visser  Christian WB Bachem 《Plant methods》2009,5(1):13-14

Background  

The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as a model for berry-fruit plants. With the progress of the tomato sequencing project, reverse genetics becomes an obvious and achievable goal.  相似文献   

Identification and validation of cold responsive microRNAs of <Emphasis Type="Italic">Hevea brasiliensis</Emphasis> using high throughput sequencing     

Linu Kuruvilla  MB Mohamed Sathik  Molly Thomas  Lisha.P Luke  Sumesh KV 《Journal of Crop Science and Biotechnology》2017,20(5):369-377

Cold stress is one of the major abiotic factors that influence the productivity and geographical distribution of many agriculturally important crops like Hevea brasiliensis. Cultivation of H. brasiliensis in India is being extended to northeastern regions, where low temperature during winter adversely affects its survival, growth, and productivity. Developing cold-tolerant genotypes is a primary requisite to maximize the productivity under such challenging environmental conditions. However, lack of methods for early evaluation of cold tolerance in the newly developed clones and the extensive time required for assessing their tolerance in the field are major constraints for clonal selection. The present study was initiated with an objective to identify and characterize cold stress responsive miRNAs from H. brasiliensis that show stronger association with cold tolerance. Next generation sequencing using Illumina HiSeq method revealed the expression of 21 and 29 conserved miRNA (from clone RRIM 600) families in cold-stressed and control samples, respectively. Forty-two novel miRNAs were identified from this study. Upon differential expression analysis, eight conserved miRNAs were found commonly expressed in both the samples. When expression analyses were performed subsequently with six selected miRNAs in two Hevea clones (viz. RRII 105 and RRIM 600), miR169 showed a strong association with cold tolerance. miRNAs such as miR482 and miR159 also exhibited association with cold tolerance. This study suggests the possibility of employing these miRNAs as markers for cold tolerance after validation in more number of genotypes with varying levels of cold tolerance.  相似文献   

Persistent anthelmintic activity of topically administered ivermectin in red deer (Cervus elaphus L.) against lungworms (Dictyocaulus viviparus)     

S. Rehbein  M. Visser 《New Zealand veterinary journal》2013,61(3):85-87

A controlled trial was conducted to evaluate the persistent anthelmintic effect of ivermectin as a topical treatment at 500 μg/kg against induced infection with lungworm (Dicryocaulus viviparus) in red deer (Cervus elaphus). The results showed a highly significant (p < 0.01) anthelmintic activity for at least 28 days against a newly acquired infection with Dictyocauus viviparus (> 99% efficacy).  相似文献   

Vaccination against foot and mouth disease: current state and perspectives     

Visser N  Thiel HJ 《DTW. Deutsche tier?rztliche Wochenschrift》2001,108(12):522-525

Foot-and-mouth disease (FMD) is endemic in many parts of the world and poses a permanent threat for cloven-hoofed animals in all countries. The available vaccines against FMD are safe and efficacious. Combat of FMD by vaccination is controversial in currently FMD-free countries including the ones of the European Union. The article summarizes our knowledge concerning production and use of vaccines, virus persistence, differentiation between vaccinated and infected animals, vaccination programs and perspectives of vaccine development.  相似文献   

Responses of horses in behavioural tests correlate with temperament assessed by riders     

Visser EK  Van Reenen CG  Rundgren M  Zetterqvist M  Morgan K  Blokhuis HJ 《Equine veterinary journal》2003,35(2):176-183

REASONS FOR PERFORMING STUDY: Behavioural tests as well as observers' ratings have been used to study horses' temperament. However, the relationship between the ratings and the responses in behavioural tests has not yet been studied in detail. OBJECTIVES: The aim of the present study was to examine this relationship between ratings and responses. METHODS: Eighteen mature Swedish Warmblood horses were subjected to 2 behavioural tests, one relating to novelty (novel object test) and one to handling (handling test). Subsequently, 16 of these horses were ridden by 16 equally experienced students, having no former experience with the horses. Immediately after each ride, the students scored the horse for 10 temperamental traits using a line rating method. RESULTS: It was shown that for each temperamental trait all 16 riders agreed on the ranking of the horses (0.212相似文献