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1.
In the hills of north–west India, maize (Zea mays L.)-wheat (Triticum aestivum L.) is the dominant cropping system. However, rainfed wheat suffers from lack of optimum moisture at sowing. Field experiments were conducted for 3 years on a silty clay loam (Typic Hapludalf) to evaluate the effectiveness of mulches and conservation tillage for rainfed wheat in mitigating this problem. The treatments were ten factorial combinations of five mulch-tillage practices and two nitrogen levels (N60 and N120 kg ha−1). Mulch treatments consisted of application of 10 Mg ha−1 (dry weight basis), to previous standing maize, of either wild sage (Lantana camara L.) or eupatorium (Eupatorium adenophorum Sprengel) in combination with either conventional or conservation (minium) tillage prior to wheat sowing. These alternative practices were compared to the conventional farmer practice of soil tillage after harvest of maize with no mulch. The application of these weed mulches to standing maize maintained friable soil structure owing to a five fold higher mean population of earthworms underneath mulch. Mulches resulted in 0.06–0.10 m3 m−3 higher moisture in the seed-zone when wheat was sown compared with the conventional farmer practice of soil tillage after maize harvest. Mulch-conservation tillage treatments favourably moderated the hydro-thermal regime for growing a wheat crop. The mean root mass density under these treatments at wheat flowering was higher by 1.27–1.40 times over the conventional farmer practice during the 3 year study. Conservation tillage holds promise because it does not require elaborate tillage and may ultimately reduce animal draught in the hilly regions. Recycling available organic materials having no fodder value coupled with conservation tillage may help enrich the soil environment in the long-term. The practice also offers gainful use of these obnoxious weeds that cause great menace in grass and forest lands in the region.  相似文献   
2.
Phytoremediation is a promising approach for reclamation of salt-affected soil. Phytoextraction is the most commonly used process, which exploits plants to absorb, immobilize, and accumulate salt in their shoots. In this study, halotolerant plant growth-promoting rhizobacteria (PGPR) were isolated from the rhizosphere of wild grasses growing naturally in salt-affected areas of Lucknow, Uttar Pradesh (India) and were tested for their efficacies of salt-tolerance and plant growth-promoting (PGP) abilities. Based on 16S rRNA sequences, the most efficient halotolerant isolates possessing PGP traits were identified as Pseudomonas plecoglossicida (KM233646), Acinetobacter calcoaceticus (KM233647), Bacillus flexus (KM233648), and Bacillus safensis (KM233652). Application of these isolates as bio-inoculants significantly (P < 0.05) increased the growth and bacoside A yield of a medicinal plant, Bacopa monnieri (L.) Nash, grown on natural salt-affected soil. The phytoremediation of salt-affected soil was evident by the substantial increase in shoot Na+:K+ ratio of bio-inoculant-treated plants. When compared to un-inoculated control plants, the soil physico-chemical properties of bio-inoculant-treated plants were improved. The shoot and root biomass (fresh and dry weights), soil enzymes, and soil nutrient parameters showed significant positive correlations with the shoot Na+:K+ ratio. Consequently, the halotolerant PGPR screened in this study could be useful for the reclamation of saline soils concomitant with improved plant growth and bacoside A yield.  相似文献   
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OBJECTIVE: To determine the in vitro sensitivity of 4 vaccine-associated feline sarcoma (VAFS) cell lines to the chemotherapeutic agents vincristine and paclitaxel. SAMPLE POPULATION: Cell lines derived from 4 VAFS specimens. PROCEDURES: Cell lines were cultured in vitro and individually exposed to various concentrations of vincristine and paclitaxel. Survival was estimated after 24 and 72 hours of exposure to each drug, and the drug concentrations that resulted in 50 and 90% reduction in number of viable cells (IC50 and IC90, respectively) were calculated. RESULTS: Both vincristine and paclitaxel had significant dose-dependent effects on the viability of the VAFS cell lines. After 72 hours of drug exposure, the IC50 and IC90 of vincristine for the 4 cell lines were between 0.005 to 0.039 microg/ml and 0.045 to 1.027 microg/ml, respectively. The IC50 and IC90 values for paclitaxel were between 0.037 to 0.092 microg/ml and 2.450 to 15.413 microg/ml, respectively. CONCLUSIONS: Results of pharmacokinetic studies on vincristine and paclitaxel in other species suggest that concentrations greater than the IC50 values may be possible for both drugs in feline patients as well. The drug concentrations at which viable cell numbers were reduced by 90% may also be attained in vivo for some cases, but detailed information is needed regarding the distribution, concentration, duration of availability, and toxicity of various drugs in cats. Carefully chosen combinations of antineoplastic agents need to be screened to identify treatment protocols that may be further evaluated clinically for the treatment of VAFS.  相似文献   
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Tricresyl phosphate (TCP) in contaminated edible oils was extracted using acetonitrile and detected by thin layer chromatography as well as gas chromatography (GC). The chromatoplate was developed with isooctane-ethyl acetate (90 + 10) and visualized by spraying with 2,6-dichloroquinone chloroimide. TCP gives a characteristic blue-violet spot when heated at 100 degrees C for 15 min. The method is direct and sensitive and can be used to detect as low as 2.5 micrograms TCP or TOCP (tri-o-cresyl phosphate). GC was carried out using 10% OV-101 as the stationary phase and flame ionization detection for confirmation and quantitation of TOCP in oils.  相似文献   
7.
Essential oils from inflorescences and roots of Eupatorium adenophorum Spreng (Asteraceae) have been investigated for their antimicrobial, phytotoxic and antioxidant activities. Based on GC–MS, the oil from inflorescences is dominated by sesquiterpenes (55.9 %) with γ-cadinene (18.4 %), γ-muurolene (11.7 %), 3-acetoxyamorpha-4,7(11)-diene-8-one (7.4 %) and bornyl acetate (6.3 %) as the major constituents. The oil obtained from the roots contained both sesquiterpenes (34.3 %) and monoterpenes (32.5 %) in almost equal proportions with E,E-cosmene (19.9 %), γ-muurolene (10.1 %), isothymol (7.5 %), β-cadinene (7.0 %) and α-phellandren-8-ol (5.9 %) as the major constituents. Both oils exhibited significant antifungal activity against five phytopathogenic fungi. The inflorescence oil showed higher antibacterial activity against Klebsiella pneumoniae, while the root oil was more effective against Staphylococcus aureus. The oils strongly inhibited or delayed germination and seedling growth of the weed Phalaris minor in a dose-dependent manner. As evidenced by a DPPH assay, the essential oils also exhibited significant free radical scavenging activity.  相似文献   
8.
We investigated the magnitude and dynamics of the eddy covariance system (ECS) residual energy (energy balance closure error) for a subsurface drip-irrigated maize (Zea mays L.) field in 2005 and 2006 growing and non-growing (dormant) seasons. The corrections for coordinate rotation, oxygen, frequency, and Webb–Pearman–Leuning corrections improved the slope of the total convective energy (latent heat + sensible heat) with respect to the net available energy (from 0.68 to 0.84), but the data filtering (for horizontal and frictional wind speeds higher than 2 m s?1 and lower than 0.2 m s?1) had little effect on the slope. Also, the number of data points available for the analyses was reduced by 53 % after filtering. Overall, the daytime residual energy varied between ?100 and 200 W m?2 during the dormant seasons and between ?500 and 600 W m?2 during the growing seasons. Most of the nighttime residual energy ranged within ±40 W m?2 during the calendar year in 2005 and within ?60 and 20 W m?2 in 2006. During nighttime, the total convective energy is vertically distributed with respect to (R n ? G), indicating that the total convective energy is independent of the variations in (R n ? G). Secondly, it was observed that nighttime residual energy did not show any seasonal variation patterns throughout the two consecutive years and confined mostly within a narrow range of ±40 W m?2, showing no dependency on seasonal changes in surface conditions. The maximum variation in residual energy was usually around frictional wind speed of 0.3–0.5 m s?1 (varying between ?150 and 300 W m?2) and then decreasing to a range of ±100 W m?2 at higher frictional wind speeds. On average, the residual energy decreased by about 33 W m?2 (after the intercept) for every 1.0 m s?1 increase in frictional wind speed, whereas the residual energy decreased by about 4 W m?2 (after the intercept) for every 1.0 m s?1 increase in horizontal wind speed. Similar diurnal residual energy distribution patterns, with different magnitudes, were observed during growing and dormant seasons. Even though a slight decrease in residual energy was observed with increase in leaf area index (LAI) in both growing seasons, LAI did not have considerable influence on the seasonal variation in the residual energy. The residual energy was also evaluated by separating the data into morning and afternoon hours. We observed that the root-mean-squared difference value is slightly greater for the morning data than the afternoon, indicating greater residual energy in the morning hours due to weaker turbulent mixing than the afternoon. Overall, significant reduction in the available evapotranspiration data after applying a series of corrections possess challenges in terms of utilization of ECS for in-season irrigation management and crop water requirement determinations that needs to be further researched and addressed.  相似文献   
9.
Asian soybean rust (ASR) caused by Phakopsora pachyrhizi severely reduces seed yield in soybean. Molecular tagging of ASR resistance can help in the process of resistance breeding. In this study, an F2 population of cross (susceptible cultivar ‘NRC 7’ × resistant exotic genotype EC 241780) was used for bulked segregant analysis (BSA) with 25 SSR (simple sequence repeat) primers linked with six Rpp genes. Among them, five polymorphic SSR markers, viz., Sct 187, SSR 1859, Satt 191 (Rpp1b like loci) and Satt 215, Sat_361 (Rpp2 loci) distinguished the ASR resistant and susceptible bulks and individuals. In combined marker analysis, the markers Satt 191 (Rpp1b like loci) and Satt 215 (Rpp2 loci) were linked with ASR severity score and were also confirmed in individual 110 F2 segregants. Hence, these markers could be utilized in the marker assisted rust resistance breeding of Rpp1b like and Rpp2 genes. In silico candidate gene analysis for hypersensitive response revealed that Satt 191 linked region was rich in genes encoding apoptotic ATPase having leucine‐rich repeat (LRR) domain.  相似文献   
10.
This study compares the effects of different irrigation regimes on seed yield and oil yield quality and water productivity of sprinkler and drip irrigated sunflower (Helianthus annus L.) on silty-clay-loam soils in 2006 and 2007 in the Mediterranean region of Turkey. In sprinkler irrigation a line-source system was used in order to create gradually varying irrigation levels. Irrigation regimes consisted of full irrigation (I1) and three deficit irrigation treatments (I2, I3 and I4), and rain-fed treatment (I5). In the drip system, irrigation regimes included full irrigation (FI-100), three deficit irrigation treatments (DI-25, DI-50, DI-75), partial root zone drying (PRD-50) and rain-fed treatment (RF). Irrigations were scheduled at weekly intervals both in sprinkler and drip irrigation, based on soil water depletion within a 0.90 m root zone in FI-100 and I1 plots. Irrigation treatments influenced significantly (P < 0.01) sunflower seed and oil yields, and oil quality both with sprinkler and drip systems. Seed yields decreased with increasing water stress levels under drip and sprinkler irrigation in both experimental years. Seed yield response to irrigation varied considerably due to differences in soil water contents and spring rainfall distribution in the experimental years. Although PRD-50 received about 36% less irrigation water as compared to FI-100, sunflower yield was reduced by an average of 15%. PRD-50 produced greater seed and oil yields than DI-50 in the drip irrigation system. Yield reduction was mainly due to less number of seeds per head and lower seed mass. Soil water deficits significantly reduced crop evapotranspiration (ET), which mainly depends on irrigation amounts. Significant linear relationships (R2 = 0.96) between ET and oil yield (Y) were obtained in each season. The seed yield response factors (kyseed) were 1.24 and 0.86 for the sprinkler and 1.19 and 1.06 for the drip system in 2006 and 2007, respectively. The oil yield response factor (kyoil) for sunflower was found to be 1.08 and 1.49 for both growing seasons for the sprinkler and 1.36 and 1.25 for the drip systems, respectively. Oil content decreased with decreasing irrigation amount. Consistently greater values of oil content were obtained from the full irrigation treatment plots. The saturated (palmitic and stearic acid) and unsaturated (oleic and linoleic acid) fatty acid contents were significantly affected by water stress. Water stress caused an increase in oleic acid with a decrease in linoleic acid contents. The palmitic and stearic acid concentrations decreased under drought conditions. Water productivity (WP) values were significantly affected by irrigation amounts and ranged from 0.40 to 0.71 kg m−3 in 2006, and from 0.69 to 0.91 kg m−3 in 2007. The PRD-50 treatment resulted in the greatest WP (1.0 kg m−3) and irrigation water productivity (IWP) (1.4 kg m−3) in both growing seasons. The results revealed that under water scarcity situation, PRD-50 in drip and I2 in sprinkler system provide acceptable irrigation strategies to increase sunflower yield and quality.  相似文献   
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