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1.
A dog with visceral leishmaniasis developed rear limb edema, and distension of the caudal epigastric veins. Glomerular disease with nephrotic syndrome and hypercoagulable state was diagnosed. Sonographically there was massive thrombosis of the caudal vena cava.  相似文献   
2.
Surveillance of hepatitis E virus (HEV) in risk groups is an important strategy to monitor its circulation pattern and to timely detect changes thereof. The aims of this cross-sectional study were to estimate the prevalence of HEV infections in pigs and humans from different regions of the country, to identify risk factors for increasing anti-HEV IgG prevalence and to characterize HEV strains. The presence of anti-HEV antibodies was assessed by commercial ELISA in serum samples from the general population, farm and slaughterhouse employees, as well as pigs sampled in the three regions of Cuba from February to September 2016. Overall, individuals with occupational exposure to swine or swine products (70/248, 28.2%) were 4 times more likely to be seropositive compared to the general population (25/285, 8.7%; OR: 4.18; p < .001). Within the risk group, risk factors included age, number of years working in a professional activity with direct exposure to swine, geographic region and distance between residence and closest professional swine setting, while wearing gloves had a protective effect. Prevalence of total anti-HEV antibodies in swine was 88.2% (165/187) and HEV RNA was detected by real-time RT-PCR in 9.2% (16/173) swine stools. All HEV strains sequenced clustered within genotype 3. Some strains clearly belonged to subtype 3a, while another group of strains was related with subtypes 3b and 3 k but partial HEV sequences did not allow unequivocal subtype assignment. These findings suggest that the high HEV exposure in Cuban individuals with swine-related occupations could be due to enzootic HEV in certain regions, direct contact with infectious animals or their products as well as environmental contamination.  相似文献   
3.
A review of protothecosis in dogs revealed that this malady usually begins in the gastrointestinal tract and progresses to systemic involvement. Clinical signs generally include bloody diarrhea or blood-stained feces as well as blindness, ataxia, and polyuria. Histologically, myriads of protothecal organisms in different stages of development are found in the granulomatous lesions. Two main species have been culturally identified: Prototheca zopfii and P wickerhamii. In the absence of cultural studies, species identification can be accomplished readily by immunofluorescence. The present case involved P zopfii infection in a 5-year-old female Cocker Spaniel that had bloody diarrhea, with a history of bloody diarrhea 6 months earlier.  相似文献   
4.
  • 1. Recreational shore fishing along the coast of the marine reserve of Cap de Creus (NW Mediterranean) was studied in 2007 and 2009 based on roving creel surveys (on‐site angler surveys during which anglers' harvests are examined by the survey clerk). The study aimed to assess the biological impacts of this leisure activity on coastal fish stocks and the potential risks arising from the use of exotic baits.
  • 2. Recreational shore fishers employ seven different fishing techniques, of which the bottom fishing rod (a fishing rod whose hooks, together with the bait, lie on the bottom or near it by means of a heavy weight) is by far the most widely used method (nearly 90% of observations). In total, 25 fish species were identified in the catch from the bottom fishing rod.
  • 3. The estimated annual shore fishing catches (c.3 tons) are much lower than those obtained by recreational boat and spear fishing (c.20 tons each), and those from commercial (artisanal) fishing (c.50 tons). The weighted mean vulnerability index and trophic level values in the catch from the bottom fishing rod are 52.2 and 4.03, respectively.
  • 4. A minimum of 43% of the baits used by the shore anglers were live, non‐native species (mostly polychaetes).
  • 5. Overall, results highlight the impact of shore angling on coastal fish communities of a protected area and the increasing environmental risks arising from the use of exotic marine baits, which constitute a potential and unregulated vector of introduction of non‐native species in the Mediterranean. Copyright © 2011 John Wiley & Sons, Ltd.
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5.
Renal dysplasia was diagnosed in a five-month-old female Brie sheepdog. Clinical signs included vomiting, diarrhoea, coughing, polyuria/ polydipsia, bone pain, carpal and tarsal deviation and small, irregular kidneys. Non-regenerative anaemia, azotaemia, isosthenuric urine and hyperphosphataemia were the most prominent laboratory abnormalities. Juvenile renal disease was diagnosed. Histopathological evaluation of the kidneys showed lesions of renal dysplasia.  相似文献   
6.
Acute intrinsic renal failure was diagnosed in a two-year-old, male, German shepherd dog following a Vipera aspis bite. Clinical signs included depression, hypersalivation, vomiting, tachypnoea, abdominal pain, splenomegaly, oliguria with haematuria and haemolysed serum. Leucocytosis with a shift to the left, thrombocytopenia, prolonged coagulation times (activated partial thromboplastin time, prothrombin time and thrombin time), hypofibrinogenaemia, azotaemia and hyposthenuria were the most prominent laboratory abnormalities. Histopathological evaluation of the kidneys showed a discrete glomerular hypercellularity, mesangial lysis and renal tubules filled with many hyaline casts and some necrotic cells.  相似文献   
7.
R. Font    B. Wittkop    A. G. Badani    M. Del  Río-Celestino  W. Friedt    W. Lühs  A. De  Haro-Bailón 《Plant Breeding》2005,124(4):410-412
Visible and near‐infrared spectroscopy (NIRS) calibrations for acid detergent fibre (ADF) in intact rapeseed (Brassica napus L.) were performed for two different sample volumes (10 ml, 500 seeds approximately; and 1 ml, 50 seeds approximately). The inclusion of brown and yellow‐seeded cultivars in this work has allowed the whole range of ADF currently described in the literature for this character to be covered. Chemometric techniques have been used for developing calibration equations for both procedures when measuring the two different seed sample volumes. On the basis of the coefficient of determination in the cross‐validation (R2cv) obtained for the 10 and 1 ml assays (0.80 and 0.73), and SECV/SEL ratios (2.30 and 2.57), respectively, both equations showed an accuracy sufficient for screening purposes in an ADF range from 6.80 to 13.46% dry wt, which is presented in this work.  相似文献   
8.
Purpose

The application of sludge from wastewater in agriculture has increased in recent years, and it is therefore important to assess the effect that such treatment has on both the soil and the plant. The aim of the study described here was to ascertain whether there is a variation in the properties of the soil and to determine if this addition has an impact on the plant.

Materials and methods

The area of investigation was close to the municipality of Villarrubia de los Ojos (Ciudad Real). In this work, six samples were taken from the surface horizon in the studied plot at a depth of 35 cm. A further three samples were taken: (i) a surface horizon of a soil close to the area under investigation but without treatment (control sample), (ii) a sample of sludge from the wastewater treatment plant and (iii) a sample of the mixture used by farmers as fertilizer. Laboratory tests were conducted in accordance with the SCS-USDA (1972) guidelines. Trace element samples were analysed by X-ray fluorescence spectrophotometry (Philips PW 2404).

Results and discussion

The parcel of land studied is dominated by a sandy texture (88.3 % sand), and a decrease in pH was observed in areas in which the mixture (manure + sludge) was added (pH?=?8.0) compared to areas in which fertilizer was not applied (pH?=?8.5). It was observed that the addition of the compound led to an increase in the electrical conductivity of the soil. The trace elements can be organized into two groups based on the results obtained in this study. One group contains the trace elements that were only present in the rows that were treated with the fertilizer. The other group of trace elements was mobilized throughout the whole plot.

Conclusions

The application of sewage sludge on agricultural soils can be very useful as an organic amendment because it produces an increase in soil organic matter. However, sewage sludge must be applied with caution due to the changes in soil chemical properties (for example, pH and E.C.). The use of this type of waste for prolonged periods of time can cause problems of contamination in the soil.

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9.
The pharmacokinetics (PK) of azithromycin after i.v. and i.m. injection at a single dosage of 20 mg/kg bodyweight was studied in sheep. Blood samples were collected from the jugular vein until 120 h after dosing for both routes. Plasma concentrations of azithromycin were determined by bioassay. The plasma concentration-time data of azithromycin best fitted a three-compartment model after i.v. administration and a two-compartment model with first-order absorption after i.m. administration. The elimination half-life (t(1/2lambdaz)) was 47.70 +/- 7.49 h after i.v. administration and 61.29 +/- 13.86 h after i.m. administration. Clearance value after i.v. dosing was 0.52 +/- 0.08 L/kg.h. After i.m. administration a peak azithromycin concentration (C(max)) of 1.26 +/- 0.19 mg/L was achieved at 1.24 +/- 0.31 h (t(max)). Area under the curve (AUC) were 38.85 +/- 5.83 mg.h/L and 36.03 +/- 1.52 mg.h/L after i.v. and i.m. administration respectively. Bioavailability obtained after i.m. administration was 94.08 +/- 11.56%. The high tolerability of this i.m. preparation and the favourable PK behaviour such as the long half-life and high bioavailability make azithromycin likely to be effective in sheep.  相似文献   
10.

Context

The role of agricultural landscapes in biodiversity conservation is an emerging topic in a world experiencing a worrying decrease of species richness. Farm systems may either decrease or increase biological diversity, depending on land-use intensities and management.

Objectives

We present an intermediate disturbance-complexity model (IDC) of cultural landscapes aimed at assessing how different levels of anthropogenic disturbance on ecosystems affect the capacity to host biodiversity depending on the land matrix heterogeneity. It is applied to the Mallorca Island, amidst the Mediterranean biodiversity hotspot.

Methods

The model uses the disturbance exerted when farmers alter the Net Primary Production through land-use change as well as when they remove a share of it (HANPP), together with Shannon–Wiener index (H′) of land-cover diversity. The model is tested with a twofold-scalar experimental design (1:50,000 and 1:5000) of a set of landscape units along three time points (1956, 1989, 2011). Species richness of breeding and wintering birds, taken as a biodiversity proxy, is used in an exploratory factor analysis.

Results

The results clearly show that when intermediate levels of HANPP are performed within intermediate levels of complexity (H′) in landscape patterns, like agro-forest mosaics, great bird species richness and high socio-ecological resilience can be maintained. Yet, these complex-heterogeneous landscapes are currently vanishing due to industrial farm intensification, rural abandonment and urban sprawl.

Conclusions

The results make apparent the usefulness of transferring the concept of intermediate disturbance-complexity interplay to cultural landscapes. Our spatial-explicit IDC model can be used as a tool for strategic environmental assessment of land-use planning.
  相似文献   
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