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1.
The mutation T3811 → G3811 (TG3811) discovered in the myostatin gene of the Blonde d’Aquitaine breed is suspected of contributing to the outstanding muscularity of this breed. An experiment was designed to estimate the effect of this mutation in an F2 and back-cross Blonde d’Aquitaine × Holstein population. By genotyping all known mutations in the myostatin gene, it was ensured that the TG3811 mutation was indeed the only known mutation segregating in this population. Fifty-six calves (43 F2, 13 back-cross) were intensively fattened and slaughtered at 24.0 ± 1.4 wk of age. The effects of the mutation were estimated by comparing the calves with the [T/T] (n = 18), [T/G] (n = 30), and [G/G] (n = 8) genotypes. Highly significant substitution effects (P < 0.001), above + 1.2 phenotypic SD, were shown on carcass yield and muscularity scores. Birth weight (P < 0.001) was positively affected by the mutation (+0.8 SD) but not growth rate (P = 0.97), while carcass length (P = 0.03), and fatness (P ≤ 0.03) were negatively affected (–0.5 to –0.7 SD). The characteristics of the Triceps brachii muscle were affected by the mutation (P < 0.001), with lower ICDH activity (oxidative) and a higher proportion of myosin type 2X muscle fibers (fast twitch). The effects of the TG3811 mutation were similar to those of other known myostatin mutations, although the Blonde d’Aquitaine animals, which are predominantly [G/G] homozygous, do not exhibit extreme double muscling.  相似文献   
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Surface irrigation rehabilitation in the South-East of France has shown success ever since the beginning of its development in the early 1980s. The gradual introduction of surface distributors, mechanized or automated has resulted in water savings and above all in decreased labor input. The distributors used are: gated pipes, layflat tubing, buried low pressure pipe and cablegation. They are used over an ever increasing area which attained about 1000 ha in 1987.Improvement is made in furrow head distribution as well as in field levelling, furrow flow evaluation, flow control at the head of the furrow and in the collective system.The effect of a modernized system and improved knowledge has been to make automated surface irrigation (particularly furrow irrigation) a modern application method in competition with techniques such as sprinklers and localized irrigation.Abbreviations ASAE American Society of Agricultural Engineers - CEMAGREF Centre d'Etude du Machinisme Agricole du Génie Rural des Eaux et des Forêts, Division Irrigation, BP 5095, 34033 Montpellier et BP 31 Le Tholonet 13612 AIX - ENGREF Ecole Nationale du Génie Rural des Eaux et des Forêts, Département Maîtrise de l'Eau et Développement, BP 5093, 34033 Montpellier - IRAT Institute de Recherche en Agronomie Tropicale, Zolad Mini Parc Bt7, 34100 Montpellier - P.V.C. Polyvinyl chloride - RNEDHA Réseau National Expérimentation Démonstration secteur Hydraulique Agricole, BP 5095, 34033 Montpellier - SCP Société du Canal de Provence et d'Aménagement de la Région Provençale, BP 100, 13603 AIX en Provence  相似文献   
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Two major proteins, Mcf-A67 and Mcf-B66, were identified by mini two-dimensional polyacrylamide gel electrophoresis in order to distinguish the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from eight other species. These quarantine proteinic markers have been microsequenced after enzymatic digestion. The internal amino acid sequences exhibit similarities to members of a family of low molecular weight intracellular lipid-binding proteins. Moreover, to explore a simple, rapid, and inexpensive way to identify the two quarantine nematodes, dot blot hybridizations were performed using an antiserum (A67) produced from the longest amino-acid sequence of the protein Mcf-A67. Although several proteins stained on the M. chitwoodi and M. fallax western blot membranes, the two nematodes were easily distinguished from other root-knot nematodes, on dot blot assays with soluble proteins extracted from a single female. Because of its specificity and sensitivity, the use of the A67 antiserum to improve the diagnosis of the two European quarantine root-knot nematodes is discussed.  相似文献   
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The multidisciplinary project “MOREST” aims to improve our understanding of causes of summer mortality in Crassostrea gigas juveniles in France and to reduce its impact on oyster production. As part of the MOREST project, 43 full-sib families nested within 17 half-sib families were produced, planted out and tested in 3 sites during summer 2001 to assess to what extent genetic variability exists for this trait. A strong genetic basis was found for survival as narrow-sense heritability estimates ranged from 0.47 to 1.08, with higher values in sites where summer mortality was higher. Genetic correlations across sites were positive and very high for survival, indicating no genotype by environment interaction. In contrast, lower genetic variation was observed for growth in all sites. Finally, genetic correlations between growth and survival were low, in all sites. Selective breeding in a single site should therefore be an efficient means of improving survival in oysters less than one year old along the French Atlantic and Channel coastlines with only very limited effects on growth. As yield mostly depends on survival and growth, this approach should significantly improve harvestable yield. Possible reasons why a high genetic variance for survival appears to be maintained in wild populations are discussed.  相似文献   
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Editorial     
Whether or not fruit buds in orchards can be protected from freezing by the use of fans, when no temperature inversion exists, depends upon the degree of bud radiant cooling at such times. Nighttime bud cooling in apple and peach orchards was estimated by comparing the temperature of buds and the adjacent air. The extent of radiant cooling was usually less than 0.6°C below ambient. Even this modest degree of radiant bud cooling appeared to require conditions with less than 60% cloud cover and wind speed less than 1 to 1.5 m s?1 at a height of 2 m. However, such conditions were also accompanied by inversions. The protective potential of fans in the absence of an inversion (postulated on the basis of bud radiant cooling) is therefore disclaimed on two accounts: (1) that the bud/air temperature difference is quite small and would only rarely span the bud critical temperature, and (2) that bud cooling would probably not occur during conditions which prevent the presence of an inversion.  相似文献   
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Rapid climate change has been implicated as a cause of evolution in poorly adapted populations. However, phenotypic plasticity provides the potential for organisms to respond rapidly and effectively to environmental change. Using a 47-year population study of the great tit (Parus major) in the United Kingdom, we show that individual adjustment of behavior in response to the environment has enabled the population to track a rapidly changing environment very closely. Individuals were markedly invariant in their response to environmental variation, suggesting that the current response may be fixed in this population. Phenotypic plasticity can thus play a central role in tracking environmental change; understanding the limits of plasticity is an important goal for future research.  相似文献   
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